[English] 日本語
EMN Papers
- Database of articles cited by 3DEM data entries -

+
Search query

Keywords
Author
Journal
IF

-
Structure paper

TitleStructural basis of Acinetobacter type IV pili targeting by an RNA virus.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 2746, Year 2024
Publish dateMar 29, 2024
AuthorsRan Meng / Zhongliang Xing / Jeng-Yih Chang / Zihao Yu / Jirapat Thongchol / Wen Xiao / Yuhang Wang / Karthik Chamakura / Zhiqi Zeng / Fengbin Wang / Ry Young / Lanying Zeng / Junjie Zhang /
PubMed AbstractAcinetobacters pose a significant threat to human health, especially those with weakened immune systems. Type IV pili of acinetobacters play crucial roles in virulence and antibiotic resistance. ...Acinetobacters pose a significant threat to human health, especially those with weakened immune systems. Type IV pili of acinetobacters play crucial roles in virulence and antibiotic resistance. Single-stranded RNA bacteriophages target the bacterial retractile pili, including type IV. Our study delves into the interaction between Acinetobacter phage AP205 and type IV pili. Using cryo-electron microscopy, we solve structures of the AP205 virion with an asymmetric dimer of maturation proteins, the native Acinetobacter type IV pili bearing a distinct post-translational pilin cleavage, and the pili-bound AP205 showing its maturation proteins adapted to pilin modifications, allowing each phage to bind to one or two pili. Leveraging these results, we develop a 20-kilodalton AP205-derived protein scaffold targeting type IV pili in situ, with potential for research and diagnostics.
External linksNat Commun / PubMed:38553443 / PubMed Central
MethodsEM (helical sym.) / EM (single particle)
Resolution3.0 - 8.55 Å
Structure data

41442

8tob

EMDB-41442, PDB-8tob:
Acinetobacter GP16 Type IV pilus
Method: EM (helical sym.) / Resolution: 3.14 Å

41443

8toc

EMDB-41443, PDB-8toc:
Acinetobacter phage AP205
Method: EM (single particle) / Resolution: 3.11 Å

EMDB-41447: AP205 binding to one Acinetobacter GP16 type iv pilus
Method: EM (single particle) / Resolution: 4.2 Å

41634

8tv9

EMDB-41634, PDB-8tv9:
Inner Mat-T4P complex
Method: EM (single particle) / Resolution: 8.15 Å

41635

8tva

EMDB-41635, PDB-8tva:
Outer Mat-T4P complex
Method: EM (single particle) / Resolution: 8.55 Å

EMDB-41646: AP205 phage Acinetobacter gp16 T4P complex
Method: EM (single particle) / Resolution: 3.6 Å

41657

8tw2

EMDB-41657, PDB-8tw2:
Acinetobacter phage AP205 T=4 VLP
Method: EM (single particle) / Resolution: 3.39 Å

41666

8twc

EMDB-41666, PDB-8twc:
Acinetobacter phage AP205 T=3 VLP
Method: EM (single particle) / Resolution: 3.0 Å

Source
  • acinetobacter genomosp. 16bj (bacteria)
  • bacteria abnormis (insect)
  • acinetobacter phage ap205 (virus)
KeywordsCELL ADHESION / T4P / Competence / VIRUS/RNA / Acinetobacter / SsRNA phage virus / VIRUS / VIRUS-RNA complex / VIRUS LIKE PARTICLE / VLP

+
About EMN Papers

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Oct 5, 2021. Nobel Prize for mechanically activated and temperature-gated ion channels

Nobel Prize for mechanically activated and temperature-gated ion channels

  • The Nobel Prize in Physiology or Medicine 2021 was awarded jointly to David Julius and Ardem Patapoutian "for their discoveries of receptors for temperature and touch."
  • EM Navigator can help to find cryo-EM structure data by both pioneers.

External links:The Nobel Prize in Physiology or Medicine 2021 - NobelPrize.org / Structure data by Ardem Patapoutian / Structure data by David Julius

+
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jul 5, 2019. Downlodablable text data

Downlodablable text data

Some data of EM Navigator services can be downloaded as text file. Software such as Excel can load the data files.

PageDataFormat
EMN Searchsearch resultCSV, TSV, or JSON
EMN statisticsdata tableCSV or TSV

Related info.:EMN Search / EMN Statistics

-
EMN Papers

Database of articles cited by 3DEM data entries

  • Database of articles cited by 3DEM data entries in EMDB and PDB
  • Using PubMed data

Related info.:EMDB / PDB / Q: What are the data sources of EM Navigator? / EM Navigator / Yorodumi Papers / Changes in new EM Navigator and Yorodumi

Read more