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Structure paper

タイトル	Structural basis of Acinetobacter type IV pili targeting by an RNA virus.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 2746, Year 2024
掲載日	2024年3月29日
著者	Ran Meng / Zhongliang Xing / Jeng-Yih Chang / Zihao Yu / Jirapat Thongchol / Wen Xiao / Yuhang Wang / Karthik Chamakura / Zhiqi Zeng / Fengbin Wang / Ry Young / Lanying Zeng / Junjie Zhang /
PubMed 要旨	Acinetobacters pose a significant threat to human health, especially those with weakened immune systems. Type IV pili of acinetobacters play crucial roles in virulence and antibiotic resistance. ...Acinetobacters pose a significant threat to human health, especially those with weakened immune systems. Type IV pili of acinetobacters play crucial roles in virulence and antibiotic resistance. Single-stranded RNA bacteriophages target the bacterial retractile pili, including type IV. Our study delves into the interaction between Acinetobacter phage AP205 and type IV pili. Using cryo-electron microscopy, we solve structures of the AP205 virion with an asymmetric dimer of maturation proteins, the native Acinetobacter type IV pili bearing a distinct post-translational pilin cleavage, and the pili-bound AP205 showing its maturation proteins adapted to pilin modifications, allowing each phage to bind to one or two pili. Leveraging these results, we develop a 20-kilodalton AP205-derived protein scaffold targeting type IV pili in situ, with potential for research and diagnostics.
リンク	Nat Commun / PubMed:38553443 / PubMed Central
手法	EM (らせん対称) / EM (単粒子)
解像度	3.0 - 8.55 Å
構造データ	41442 8tob EMDB-41442, PDB-8tob: Acinetobacter GP16 Type IV pilus 手法: EM (らせん対称) / 解像度: 3.14 Å 41443 8toc EMDB-41443, PDB-8toc: Acinetobacter phage AP205 手法: EM (単粒子) / 解像度: 3.11 Å EMDB-41447: AP205 binding to one Acinetobacter GP16 type iv pilus 手法: EM (単粒子) / 解像度: 4.2 Å 41634 8tv9 EMDB-41634, PDB-8tv9: Inner Mat-T4P complex 手法: EM (単粒子) / 解像度: 8.15 Å 41635 8tva EMDB-41635, PDB-8tva: Outer Mat-T4P complex 手法: EM (単粒子) / 解像度: 8.55 Å EMDB-41646: AP205 phage Acinetobacter gp16 T4P complex 手法: EM (単粒子) / 解像度: 3.6 Å 41657 8tw2 EMDB-41657, PDB-8tw2: Acinetobacter phage AP205 T=4 VLP 手法: EM (単粒子) / 解像度: 3.39 Å 41666 8twc EMDB-41666, PDB-8twc: Acinetobacter phage AP205 T=3 VLP 手法: EM (単粒子) / 解像度: 3.0 Å
由来	acinetobacter genomosp. 16bj (バクテリア) bacteria abnormis (昆虫) acinetobacter phage ap205 (ファージ)
キーワード	CELL ADHESION (細胞接着) / T4P / Competence / VIRUS/RNA / Acinetobacter (アシネトバクター) / SsRNA phage virus / VIRUS (ウイルス) / VIRUS-RNA complex / VIRUS LIKE PARTICLE (ウイルス様粒子) / VLP