+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-17873 | ||||||||||||
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タイトル | Composite structure of Dynein-Dynactin-JIP3-LIS1 | ||||||||||||
マップデータ | Composite map of Dynein-Dynactin-JIP3-LIS1 filtered to 10 angstrom resolution | ||||||||||||
試料 |
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キーワード | Dynein (ダイニン) / AAA-Atpase / p150 / LIS1 / MOTOR PROTEIN (モータータンパク質) / Dynactin (ダイナクチン) / JIP3 | ||||||||||||
機能・相同性 | 機能・相同性情報 Regulation of PLK1 Activity at G2/M Transition / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / intracellular transport of viral protein in host cell / corpus callosum morphogenesis / 分泌 / establishment of planar polarity of embryonic epithelium ...Regulation of PLK1 Activity at G2/M Transition / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / intracellular transport of viral protein in host cell / corpus callosum morphogenesis / 分泌 / establishment of planar polarity of embryonic epithelium / microtubule cytoskeleton organization involved in establishment of planar polarity / ameboidal-type cell migration / interneuron migration / 1-alkyl-2-acetylglycerophosphocholine esterase complex / positive regulation of neuromuscular junction development / retrograde axonal transport of mitochondrion / centriolar subdistal appendage / Gap junction degradation / Formation of annular gap junctions / Regulation of actin dynamics for phagocytic cup formation / EPHB-mediated forward signaling / VEGFA-VEGFR2 Pathway / Cell-extracellular matrix interactions / RHO GTPases Activate WASPs and WAVEs / MAP2K and MAPK activation / maintenance of centrosome location / microtubule sliding / ダイナクチン / positive regulation of non-motile cilium assembly / Clathrin-mediated endocytosis / centriole-centriole cohesion / transport along microtubule / intraciliary retrograde transport / visual behavior / platelet activating factor metabolic process / dynein light chain binding / ventral spinal cord development / microtubule anchoring at centrosome / WASH complex / acrosome assembly / radial glia-guided pyramidal neuron migration / microtubule organizing center organization / F-actin capping protein complex / mitocytosis / cerebral cortex neuron differentiation / dynein heavy chain binding / negative regulation of filopodium assembly / positive regulation of intracellular transport / central region of growth cone / positive regulation of embryonic development / reelin-mediated signaling pathway / regulation of metaphase plate congression / establishment of centrosome localization / anterograde axonal protein transport / positive regulation of cytokine-mediated signaling pathway / motile cilium assembly / cortical microtubule organization / cellular response to cytochalasin B / establishment of spindle localization / Activation of BIM and translocation to mitochondria / melanosome transport / astral microtubule / cytoskeleton-dependent cytokinesis / positive regulation of spindle assembly / ciliary tip / layer formation in cerebral cortex / regulation of transepithelial transport / retromer complex / nuclear membrane disassembly / structural constituent of postsynaptic actin cytoskeleton / auditory receptor cell development / morphogenesis of a polarized epithelium / microtubule plus-end / positive regulation of dendritic spine morphogenesis / vesicle transport along microtubule / positive regulation of microtubule nucleation / postsynaptic actin cytoskeleton / protein localization to adherens junction / Intraflagellar transport / dense body / Tat protein binding / stem cell division / negative regulation of nitric oxide biosynthetic process / Neutrophil degranulation / MAP-kinase scaffold activity / negative regulation of phosphorylation / stereocilium / myeloid leukocyte migration / P-body assembly / dynein complex / regulation of G protein-coupled receptor signaling pathway / COPI-independent Golgi-to-ER retrograde traffic / apical protein localization / microtubule plus-end binding / microtubule-dependent intracellular transport of viral material towards nucleus / minus-end-directed microtubule motor activity / barbed-end actin filament capping / cytoplasmic dynein complex / retrograde axonal transport / negative regulation of JNK cascade 類似検索 - 分子機能 | ||||||||||||
生物種 | Sus scrofa (ブタ) / Homo sapiens (ヒト) | ||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 10.0 Å | ||||||||||||
データ登録者 | Singh K / Lau CK / Manigrasso G / Gassmann R / Carter AP | ||||||||||||
資金援助 | 英国, European Union, 3件
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引用 | ジャーナル: Science / 年: 2024 タイトル: Molecular mechanism of dynein-dynactin complex assembly by LIS1. 著者: Kashish Singh / Clinton K Lau / Giulia Manigrasso / José B Gama / Reto Gassmann / Andrew P Carter / 要旨: Cytoplasmic dynein is a microtubule motor vital for cellular organization and division. It functions as a ~4-megadalton complex containing its cofactor dynactin and a cargo-specific coiled-coil ...Cytoplasmic dynein is a microtubule motor vital for cellular organization and division. It functions as a ~4-megadalton complex containing its cofactor dynactin and a cargo-specific coiled-coil adaptor. However, how dynein and dynactin recognize diverse adaptors, how they interact with each other during complex formation, and the role of critical regulators such as lissencephaly-1 (LIS1) protein (LIS1) remain unclear. In this study, we determined the cryo-electron microscopy structure of dynein-dynactin on microtubules with LIS1 and the lysosomal adaptor JIP3. This structure reveals the molecular basis of interactions occurring during dynein activation. We show how JIP3 activates dynein despite its atypical architecture. Unexpectedly, LIS1 binds dynactin's p150 subunit, tethering it along the length of dynein. Our data suggest that LIS1 and p150 constrain dynein-dynactin to ensure efficient complex formation. | ||||||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_17873.map.gz | 917.7 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-17873-v30.xml emd-17873.xml | 45.2 KB 45.2 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_17873.png | 45.7 KB | ||
Filedesc metadata | emd-17873.cif.gz | 14.5 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-17873 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-17873 | HTTPS FTP |
-関連構造データ
関連構造データ | 8ptkMC 8pqvC 8pqwC 8pqyC 8pqzC 8pr0C 8pr1C 8pr2C 8pr3C 8pr4C 8pr5C C: 同じ文献を引用 (文献) M: このマップから作成された原子モデル |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_17873.map.gz / 形式: CCP4 / 大きさ: 1000 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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注釈 | Composite map of Dynein-Dynactin-JIP3-LIS1 filtered to 10 angstrom resolution | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.059 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-試料の構成要素
+全体 : Composite structure of Dynein-Dynactin-JIP3-LIS1
+超分子 #1: Composite structure of Dynein-Dynactin-JIP3-LIS1
+超分子 #2: Dynactin subunits
+超分子 #3: Dynein and JIP3
+分子 #1: Platelet-activating factor acetylhydrolase IB subunit beta
+分子 #2: ARP1 actin related protein 1 homolog A
+分子 #3: Actin, cytoplasmic 1
+分子 #4: Arp11
+分子 #5: Capping protein (Actin filament) muscle Z-line, alpha 1
+分子 #6: F-actin-capping protein subunit beta
+分子 #7: Dynactin subunit 2
+分子 #8: Dynactin subunit 3
+分子 #9: Dynactin subunit 1
+分子 #10: Dynactin 6
+分子 #11: Dynactin subunit 5
+分子 #12: C-Jun-amino-terminal kinase-interacting protein 3
+分子 #13: Dynactin subunit 4
+分子 #14: Dynein light chain 1, cytoplasmic
+分子 #15: Cytoplasmic dynein 1 heavy chain 1
+分子 #16: Cytoplasmic dynein 1 intermediate chain 2
+分子 #17: Cytoplasmic dynein 1 light intermediate chain 2
+分子 #18: Dynein light chain Tctex-type 1
+分子 #19: Dynein light chain roadblock-type 1
+分子 #20: ADENOSINE-5'-DIPHOSPHATE
+分子 #21: ADENOSINE-5'-TRIPHOSPHATE
+分子 #22: ZINC ION
+分子 #23: MAGNESIUM ION
+分子 #24: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.2 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELDBright-field microscopy / 最大 デフォーカス(公称値): 4.0 µm / 最小 デフォーカス(公称値): 0.5 µm |
撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 平均電子線量: 53.0 e/Å2 |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
-画像解析
初期モデル | モデルのタイプ: INSILICO MODEL |
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初期 角度割当 | タイプ: ANGULAR RECONSTITUTION |
最終 角度割当 | タイプ: ANGULAR RECONSTITUTION |
最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 10.0 Å / 解像度の算出法: OTHER / 使用した粒子像数: 700290 |