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Structure paper

TitleStructural basis of Acinetobacter type IV pili targeting by an RNA virus.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 2746, Year 2024
Publish dateMar 29, 2024
AuthorsRan Meng / Zhongliang Xing / Jeng-Yih Chang / Zihao Yu / Jirapat Thongchol / Wen Xiao / Yuhang Wang / Karthik Chamakura / Zhiqi Zeng / Fengbin Wang / Ry Young / Lanying Zeng / Junjie Zhang /
PubMed AbstractAcinetobacters pose a significant threat to human health, especially those with weakened immune systems. Type IV pili of acinetobacters play crucial roles in virulence and antibiotic resistance. ...Acinetobacters pose a significant threat to human health, especially those with weakened immune systems. Type IV pili of acinetobacters play crucial roles in virulence and antibiotic resistance. Single-stranded RNA bacteriophages target the bacterial retractile pili, including type IV. Our study delves into the interaction between Acinetobacter phage AP205 and type IV pili. Using cryo-electron microscopy, we solve structures of the AP205 virion with an asymmetric dimer of maturation proteins, the native Acinetobacter type IV pili bearing a distinct post-translational pilin cleavage, and the pili-bound AP205 showing its maturation proteins adapted to pilin modifications, allowing each phage to bind to one or two pili. Leveraging these results, we develop a 20-kilodalton AP205-derived protein scaffold targeting type IV pili in situ, with potential for research and diagnostics.
External linksNat Commun / PubMed:38553443 / PubMed Central
MethodsEM (helical sym.) / EM (single particle)
Resolution3.0 - 8.55 Å
Structure data

41442

8tob

EMDB-41442, PDB-8tob:
Acinetobacter GP16 Type IV pilus
Method: EM (helical sym.) / Resolution: 3.14 Å

41443

8toc

EMDB-41443, PDB-8toc:
Acinetobacter phage AP205
Method: EM (single particle) / Resolution: 3.11 Å

EMDB-41447: AP205 binding to one Acinetobacter GP16 type iv pilus
Method: EM (single particle) / Resolution: 4.2 Å

41634

8tv9

EMDB-41634, PDB-8tv9:
Inner Mat-T4P complex
Method: EM (single particle) / Resolution: 8.15 Å

41635

8tva

EMDB-41635, PDB-8tva:
Outer Mat-T4P complex
Method: EM (single particle) / Resolution: 8.55 Å

EMDB-41646: AP205 phage Acinetobacter gp16 T4P complex
Method: EM (single particle) / Resolution: 3.6 Å

41657

8tw2

EMDB-41657, PDB-8tw2:
Acinetobacter phage AP205 T=4 VLP
Method: EM (single particle) / Resolution: 3.39 Å

41666

8twc

EMDB-41666, PDB-8twc:
Acinetobacter phage AP205 T=3 VLP
Method: EM (single particle) / Resolution: 3.0 Å

Source
  • acinetobacter genomosp. 16bj (bacteria)
  • bacteria abnormis (insect)
  • acinetobacter phage ap205 (virus)
KeywordsCELL ADHESION / T4P / Competence / VIRUS/RNA / Acinetobacter / SsRNA phage virus / VIRUS / VIRUS-RNA complex / VIRUS LIKE PARTICLE / VLP

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