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Structure paper

TitleThe catalytic triad of rice NARROW LEAF1 involves H234.
Journal, issue, pagesNat Plants, Year 2024
Publish dateApr 10, 2024
AuthorsLing-Yun Huang / Na-Nv Liu / Wei-Fei Chen / Xia Ai / Hai-Hong Li / Ze-Lin Zhang / Xi-Miao Hou / Philippe Fossé / Olivier Mauffret / Dong-Sheng Lei / Stephane Rety / Xu-Guang Xi /
PubMed AbstractNARROW LEAF1 (NAL1) exerts a multifaceted influence on leaf morphology and crop yield. Recent crystal study proposed that histidine 233 (H233) is part of the catalytic triad. Here we report that ...NARROW LEAF1 (NAL1) exerts a multifaceted influence on leaf morphology and crop yield. Recent crystal study proposed that histidine 233 (H233) is part of the catalytic triad. Here we report that unlike suggested previously, H234 instead of H233 is a component of the catalytic triad alongside residues D291 and S385 in NAL1. Remarkably, residue 233 unexpectedly plays a pivotal role in regulating NAL1's proteolytic activity. These findings establish a strong foundation for utilizing NAL1 in breeding programs aimed at improving crop yield.
External linksNat Plants / PubMed:38600265
MethodsEM (single particle) / X-ray diffraction
Resolution1.75 - 3.29 Å
Structure data

17766

8pn1

EMDB-17766, PDB-8pn1:
CryoEM structure of Nal1 protein, allele SPIKE, from Oryza sativa japonica group
Method: EM (single particle) / Resolution: 2.4 Å

17768

8pn2

EMDB-17768, PDB-8pn2:
CryoEM structure of Nal1 protein, allele IR64, from Oryza sativa indica cultivar
Method: EM (single particle) / Resolution: 2.63 Å

PDB-8pme:
Structure of Nal1 indica cultivar IR64, construct 88-458
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-8pmg:
Structure of Nal1 indica cultivar IR64, construct 36-458
Method: X-RAY DIFFRACTION / Resolution: 3.29 Å

PDB-8pmi:
Structure of Nal1 indica cultivar IR64, construct 36-458 in presence of peptide from FZP protein
Method: X-RAY DIFFRACTION / Resolution: 2.83 Å

PDB-8pml:
Structure of Nal1 protein , SPIKE allele from japonica rice, construct 46-458
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

PDB-8pmm:
Structure of Nal1 protein, allele SPIKE from japonica rice, construct 31-458
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-SIN:
SUCCINIC ACID / Succinic acid

ChemComp-HOH:
WATER / Water

ChemComp-PO4:
PHOSPHATE ION / Phosphate

ChemComp-NA:
Unknown entry

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-NH4:
AMMONIUM ION / Ammonium

Source
  • oryza sativa japonica group (Japanese rice)
  • oryza sativa indica group (long-grained rice)
KeywordsPLANT PROTEIN / Serine protease

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