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キーワード
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

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Structure paper

タイトル	Molecular mechanism of dynein-dynactin complex assembly by LIS1.
ジャーナル・号・ページ	Science, Vol. 383, Issue 6690, Page eadk8544, Year 2024
掲載日	2024年3月29日
著者	Kashish Singh / Clinton K Lau / Giulia Manigrasso / José B Gama / Reto Gassmann / Andrew P Carter /
PubMed 要旨	Cytoplasmic dynein is a microtubule motor vital for cellular organization and division. It functions as a ~4-megadalton complex containing its cofactor dynactin and a cargo-specific coiled-coil ...Cytoplasmic dynein is a microtubule motor vital for cellular organization and division. It functions as a ~4-megadalton complex containing its cofactor dynactin and a cargo-specific coiled-coil adaptor. However, how dynein and dynactin recognize diverse adaptors, how they interact with each other during complex formation, and the role of critical regulators such as lissencephaly-1 (LIS1) protein (LIS1) remain unclear. In this study, we determined the cryo-electron microscopy structure of dynein-dynactin on microtubules with LIS1 and the lysosomal adaptor JIP3. This structure reveals the molecular basis of interactions occurring during dynein activation. We show how JIP3 activates dynein despite its atypical architecture. Unexpectedly, LIS1 binds dynactin's p150 subunit, tethering it along the length of dynein. Our data suggest that LIS1 and p150 constrain dynein-dynactin to ensure efficient complex formation.
リンク	Science / PubMed:38547289 / PubMed Central
手法	EM (単粒子)
解像度	3.5 - 10.0 Å
構造データ	17825 8pqv EMDB-17825, PDB-8pqv: Cytoplasmic dynein-1 motor domain in post-powerstroke state 手法: EM (単粒子) / 解像度: 4.0 Å 17826 8pqw EMDB-17826, PDB-8pqw: Cytoplasmic dynein-1 motor domain bound to dynactin-p150glued and LIS1 手法: EM (単粒子) / 解像度: 4.2 Å 17828 8pqy EMDB-17828, PDB-8pqy: Cytoplasmic dynein-1 motor domain bound to LIS1 手法: EM (単粒子) / 解像度: 3.8 Å 17829 8pqz EMDB-17829, PDB-8pqz: Cytoplasmic dynein-1 A1/A2 motor domains bound to LIS1 手法: EM (単粒子) / 解像度: 5.5 Å 17830 8pr0 EMDB-17830, PDB-8pr0: Cytoplasmic dynein-A heavy chain bound to dynactin-p150glued and IC-LC tower 手法: EM (単粒子) / 解像度: 9.4 Å 17831 8pr1 EMDB-17831, PDB-8pr1: Cytoplasmic dynein-B heavy chain bound to IC-LC tower 手法: EM (単粒子) / 解像度: 8.2 Å 17832 8pr2 EMDB-17832, PDB-8pr2: Cytoplasmic dynein-1 heavy chain bound to JIP3-LZI 手法: EM (単粒子) / 解像度: 3.8 Å 17833 8pr3 EMDB-17833, PDB-8pr3: Cytoplasmic dynein-1 heavy chain bound to JIP3-RH1 手法: EM (単粒子) / 解像度: 3.9 Å 17834 8pr4 EMDB-17834, PDB-8pr4: Dynactin pointed end bound to JIP3 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-17835: Consensus cryo-EM structure of Dynein-Dynactin-JIP3(1-185)-LIS1 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-17836: Consensus cryo-EM structure of Dynein-dynactin-JIP3(1-560)-LIS1 手法: EM (単粒子) / 解像度: 4.9 Å 17873 8ptk EMDB-17873, PDB-8ptk: Composite structure of Dynein-Dynactin-JIP3-LIS1 手法: EM (単粒子) / 解像度: 10.0 Å PDB-8pr5: Structure of the autoinhibited dynactin p150glued projection 手法: ELECTRON MICROSCOPY / 解像度: 8.6 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-ZN: Unknown entry ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体*YM
由来	homo sapiens (ヒト) sus scrofa (ブタ)
キーワード	MOTOR PROTEIN (モータータンパク質) / Dynein (ダイニン) / AAA-Atpase / p150 / LIS1 / dynactin (ダイナクチン) / LC8 / TCTEX1 / JIP3