+Open data
-Basic information
Entry | Database: PDB / ID: 2aej | ||||||
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Title | Crystal Structures of the MthK RCK Domain in no Ca2+ bound form | ||||||
Components | Calcium-gated potassium channel mthK | ||||||
Keywords | metal transport / membrane protein / Rossmann fold / helix-turn-helix / flexible interface / bilobed architecture | ||||||
Function / homology | Function and homology information monoatomic cation transmembrane transporter activity / potassium ion transport / identical protein binding / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | Methanothermobacter thermautotrophicus (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å | ||||||
Authors | Dong, J. / Shi, N. / Berke, I. / Chen, L. / Jiang, Y. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2005 Title: Structures of the MthK RCK Domain and the Effect of Ca2+ on Gating Ring Stability Authors: Dong, J. / Shi, N. / Berke, I. / Chen, L. / Jiang, Y. #1: Journal: Nature / Year: 2002 Title: Crystal structure and mechanism of a calcium-gated potassium channel Authors: Jiang, Y. / Lee, A. / Chen, J. / Cadene, M. / Chait, B.T. / MacKinnon, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2aej.cif.gz | 103.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2aej.ent.gz | 79 KB | Display | PDB format |
PDBx/mmJSON format | 2aej.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ae/2aej ftp://data.pdbj.org/pub/pdb/validation_reports/ae/2aej | HTTPS FTP |
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-Related structure data
Related structure data | 2aefC 2aemC 1lnqS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 25914.584 Da / Num. of mol.: 2 / Fragment: isoform Soluble Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanothermobacter thermautotrophicus (archaea) Gene: mthK / Plasmid: pQE70 / Production host: Escherichia coli (E. coli) / Strain (production host): M15 / References: UniProt: O27564 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 40 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.5 Details: PEG 4000, Ammonium formate, sodium acetate , pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.9911 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Dec 10, 2004 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9911 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→50 Å / Num. all: 24990 / Num. obs: 24915 / % possible obs: 99.7 % / Observed criterion σ(I): 0 / Redundancy: 3.6 % / Rmerge(I) obs: 0.052 / Rsym value: 0.052 / Net I/σ(I): 24 |
Reflection shell | Resolution: 2.1→2.18 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.384 / Mean I/σ(I) obs: 3.35 / Num. unique all: 2427 / Rsym value: 0.384 / % possible all: 98.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: pdb entry 1LNQ Resolution: 2.1→50 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.1→50 Å
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