- EMDB-11579: Outer Dynein Arm-Shulin complex - Shulin region from Tetrahymena ... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-11579
タイトル
Outer Dynein Arm-Shulin complex - Shulin region from Tetrahymena thermophila
マップデータ
試料
複合体: Shulin region from Outer Dynein Arm-Shulin complex (Tetrahymena thermophila). Contains Shulin (excluding C3 finger), Lc8e, Lc8f, Lc8d, Lc8, Lc2a, Lc9, Dic N-termini and portion of Dyh3 tail.
複合体: Dyneinダイニン
タンパク質・ペプチド: x 9種
複合体: Shulin樹林区
タンパク質・ペプチド: x 1種
リガンド: x 1種
キーワード
Cilia (繊毛) / Microtubules (微小管) / Motor (機関 (機械)) / Complex / MOTOR PROTEIN (モータータンパク質)
機能・相同性
機能・相同性情報
outer dynein arm / outer dynein arm assembly / dynein light chain binding / cilium movement / dynein heavy chain binding / axonemal dynein complex / dynein complex / minus-end-directed microtubule motor activity / cytoplasmic dynein complex / dynein light intermediate chain binding ...outer dynein arm / outer dynein arm assembly / dynein light chain binding / cilium movement / dynein heavy chain binding / axonemal dynein complex / dynein complex / minus-end-directed microtubule motor activity / cytoplasmic dynein complex / dynein light intermediate chain binding / 繊毛 / dynein intermediate chain binding / microtubule-based movement / microtubule-based process / 微小管 / ATP hydrolysis activity / ATP binding / 細胞質 類似検索 - 分子機能
Uncharacterized protein C20orf194-like / Dynein light chain Tctex-1 like / Tctex-1-like superfamily / Tctex-1 family / Dynein light chain type 1 / Dynein light chain, type 1/2 / Dynein light chain superfamily / Dynein light chain type 1 / Dynein heavy chain, AAA 5 extension domain / Dynein heavy chain AAA lid domain ...Uncharacterized protein C20orf194-like / Dynein light chain Tctex-1 like / Tctex-1-like superfamily / Tctex-1 family / Dynein light chain type 1 / Dynein light chain, type 1/2 / Dynein light chain superfamily / Dynein light chain type 1 / Dynein heavy chain, AAA 5 extension domain / Dynein heavy chain AAA lid domain / Dynein heavy chain 3, AAA+ lid domain / AAA+ lid domain / Dynein heavy chain, C-terminal domain / Dynein heavy chain, C-terminal domain, barrel region / Dynein heavy chain C-terminal domain / P-loop containing dynein motor region / Dynein heavy chain, tail / Dynein heavy chain, N-terminal region 1 / Dynein heavy chain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, linker / Dynein heavy chain, AAA module D4 / Dynein heavy chain, coiled coil stalk / Dynein heavy chain, hydrolytic ATP-binding dynein motor region / Dynein heavy chain, ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Dynein heavy chain AAA lid domain superfamily / Dynein heavy chain, domain 2, N-terminal / Dynein heavy chain, linker, subdomain 3 / Dynein heavy chain, AAA1 domain, small subdomain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, N-terminal region 2 / Hydrolytic ATP binding site of dynein motor region / Microtubule-binding stalk of dynein motor / P-loop containing dynein motor region D4 / ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40リピート / WD40リピート / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
Dynein light chain tctex-type 1 protein / Dynein intermediate chain 2 / Dynein light chain 2A / Dynein heavy chain, outer arm protein / Dynein light chain / Uncharacterized protein / Flagellar outer dynein arm intermediate protein, putative / Dynein light chain / Dynein light chain / Dynein light chain 類似検索 - 構成要素
ジャーナル: Science / 年: 2021 タイトル: Shulin packages axonemal outer dynein arms for ciliary targeting. 著者: Girish R Mali / Ferdos Abid Ali / Clinton K Lau / Farida Begum / Jérôme Boulanger / Jonathan D Howe / Zhuo A Chen / Juri Rappsilber / Mark Skehel / Andrew P Carter / 要旨: The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to ...The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to cilia by an unknown mechanism. Here, we used the ciliate to identify two factors (Q22YU3 and Q22MS1) that bind ODAs in the cytoplasm and are required for ODA delivery to cilia. Q22YU3, which we named Shulin, locked the ODA motor domains into a closed conformation and inhibited motor activity. Cryo-electron microscopy revealed how Shulin stabilized this compact form of ODAs by binding to the dynein tails. Our findings provide a molecular explanation for how newly assembled dyneins are packaged for delivery to the cilia.