EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of the American mink ACE2 binding by Y453F trimeric spike glycoproteins of SARS-CoV-2.
ジャーナル・号・ページ	J Med Virol, Vol. 95, Issue 10, Page e29163, Year 2023
掲載日	2023-10-??
著者	Hyunjun Ahn / Brenda M Calderon / Xiaoyu Fan / Yunrong Gao / Natalie L Horgan / Nannan Jiang / Dylan S Blohm / Jaber Hossain / Nicole Wedad K Rayyan / Sarah H Osman / Xudong Lin / Michael Currier / John Steel / David E Wentworth / Bin Zhou / Bo Liang /
PubMed 要旨	Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) enters the host cell by binding to angiotensin-converting enzyme 2 (ACE2). While evolutionarily conserved, ACE2 receptors differ across ...Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) enters the host cell by binding to angiotensin-converting enzyme 2 (ACE2). While evolutionarily conserved, ACE2 receptors differ across various species and differential interactions with Spike (S) glycoproteins of SARS-CoV-2 viruses impact species specificity. Reverse zoonoses led to SARS-CoV-2 outbreaks on multiple American mink (Mustela vison) farms during the pandemic and gave rise to mink-associated S substitutions known for transmissibility between mink and zoonotic transmission to humans. In this study, we used bio-layer interferometry (BLI) to discern the differences in binding affinity between multiple human and mink-derived S glycoproteins of SARS-CoV-2 and their respective ACE2 receptors. Further, we conducted a structural analysis of a mink variant S glycoprotein and American mink ACE2 (mvACE2) using cryo-electron microscopy (cryo-EM), revealing four distinct conformations. We discovered a novel intermediary conformation where the mvACE2 receptor is bound to the receptor-binding domain (RBD) of the S glycoprotein in a "down" position, approximately 34° lower than previously reported "up" RBD. Finally, we compared residue interactions in the S-ACE2 complex interface of S glycoprotein conformations with varying RBD orientations. These findings provide valuable insights into the molecular mechanisms of SARS-CoV-2 entry.
リンク	J Med Virol / PubMed:37842796
手法	EM (単粒子)
解像度	3.36 - 3.87 Å
構造データ	40976 8t20 EMDB-40976, PDB-8t20: Cryo-EM structure of mink variant Y453F trimeric spike protein bound to two mink ACE2 receptors 手法: EM (単粒子) / 解像度: 3.36 Å 40977 8t21 EMDB-40977, PDB-8t21: Cryo-EM structure of mink variant Y453F trimeric spike protein 手法: EM (単粒子) / 解像度: 3.6 Å 40978 8t22 EMDB-40978, PDB-8t22: Cryo-EM structure of mink variant Y453F trimeric spike protein bound to one mink ACE2 receptors at downRBD conformation 手法: EM (単粒子) / 解像度: 3.83 Å 40979 8t23 EMDB-40979, PDB-8t23: Cryo-EM structure of the RBD-ACE2 interface of the SARS-CoV-2 trimeric spike protein bound to ACE2 receptor after local refinement at upRBD conformation 手法: EM (単粒子) / 解像度: 3.87 Å 40980 8t25 EMDB-40980, PDB-8t25: Cryo-EM structure of the RBD-ACE2 interface of the SARS-CoV-2 trimeric spike protein bound to ACE2 receptor after local refinement at downRBD conformation. 手法: EM (単粒子) / 解像度: 3.62 Å 41143 8taz EMDB-41143, PDB-8taz: Cryo-EM structure of mink variant Y453F trimeric spike protein bound to one mink ACE2 receptors 手法: EM (単粒子) / 解像度: 3.75 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	severe acute respiratory syndrome coronavirus 2 (SARSコロナウイルス2) neovison vison (哺乳類) homo sapiens (ヒト) neogale vison (哺乳類)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / Coronavirus (オルトコロナウイルス亜科) / Spike / ACE2 (アンジオテンシン変換酵素2) / Mink / Protein binding (タンパク質) / SARS-CoV-2 (SARSコロナウイルス2)