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-Structure paper
| タイトル | Structure and replication cycle of a virus infecting climate-modulating alga . |
|---|---|
| ジャーナル・号・ページ | Sci Adv, Vol. 10, Issue 15, Page eadk1954, Year 2024 |
| 掲載日 | 2024年4月12日 |
 著者 | Miroslav Homola / Carina R Büttner / Tibor Füzik / Pavel Křepelka / Radka Holbová / Jiří Nováček / Marten L Chaillet / Jakub Žák / Danyil Grybchuk / Friedrich Förster / William H Wilson / Declan C Schroeder / Pavel Plevka /     |
| PubMed 要旨 | The globally distributed marine alga has cooling effect on the Earth's climate. The population density of is restricted by viruses, including virus 201 (EhV-201). Despite the impact of viruses ...The globally distributed marine alga has cooling effect on the Earth's climate. The population density of is restricted by viruses, including virus 201 (EhV-201). Despite the impact of viruses on the climate, there is limited information about their structure and replication. Here, we show that the dsDNA genome inside the EhV-201 virion is protected by an inner membrane, capsid, and outer membrane. EhV-201 virions infect by using fivefold vertices to bind to and fuse the virus' inner membrane with the cell plasma membrane. Progeny virions assemble in the cytoplasm at the surface of endoplasmic reticulum-derived membrane segments. Genome packaging initiates synchronously with the capsid assembly and completes through an aperture in the forming capsid. The genome-filled capsids acquire an outer membrane by budding into intracellular vesicles. EhV-201 infection induces a loss of surface protective layers from cells, which enables the continuous release of virions by exocytosis. |
 リンク | Sci Adv / PubMed:38598627 / PubMed Central |
| 手法 | EM (サブトモグラム平均) / EM (単粒子) |
| 解像度 | 12.0 - 25.0 Å |
| 構造データ |  EMDB-17649: Cryo-EM structure of the Emiliania huxleyi virus 201 (EhV-201) virion vertex with a diameter of 50 nm.  EMDB-17650: Cryo-EM structure of the Emiliania huxleyi virus 201 (EhV-201) virion vertex with a diameter of 50 nm.  EMDB-17651: Cryo-EM structure of the Emiliania huxleyi virus 201 (EhV-201) virion vertex with a diameter of 120 nm. EMDB-19035: Composite map of the Emiliania huxleyi virus 201 (EhV-201) symmetry expanded from cryo-EM structure of virion vertex 120 nm in diameter. EMDB-19036: Cryo-EM structure of the Emiliania huxleyi virus 201 (EhV-201) virion vertex with a diameter of 50 nm and a mask applied on the capsid layer. |
| 由来 |
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 キーワード |  VIRUS (ウイルス) / cryo-EM (低温電子顕微鏡法) / subtomogram averaging / EhV-201 / enveloped virus (エンベロープ (ウイルス)) / capsid (カプシド) / major capsid protein / composite map |
emiliania huxleyi virus 201 (ウイルス)
