+Open data
-Basic information
Entry | Database: PDB / ID: 8w9v | ||||||
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Title | structure of TaHKT2;1 in KCl at 2.9 Angstroms resolution | ||||||
Components | HKT2 | ||||||
Keywords | TRANSPORT PROTEIN / HKT / ion selectivity / salt tolerance | ||||||
Function / homology | Cation transporter / Cation transport protein / metal ion transport / inorganic cation transmembrane transport / monoatomic cation transmembrane transporter activity / plasma membrane / : / Uncharacterized protein Function and homology information | ||||||
Biological species | Triticum aestivum (bread wheat) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.9 Å | ||||||
Authors | Wang, J.Q. | ||||||
Funding support | China, 1items
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Citation | Journal: To Be Published Title: structure of TaHKT2;1 in KCl at 2.9 Angstroms resolution Authors: Wang, J.Q. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8w9v.cif.gz | 173.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8w9v.ent.gz | 134.4 KB | Display | PDB format |
PDBx/mmJSON format | 8w9v.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w9/8w9v ftp://data.pdbj.org/pub/pdb/validation_reports/w9/8w9v | HTTPS FTP |
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-Related structure data
Related structure data | 37382MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 60200.590 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Triticum aestivum (bread wheat) / Production host: Homo sapiens (human) / References: UniProt: A0A3B6RK40 #2: Chemical | ChemComp-K / Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: structure of TaHKT2;1 in KCl at 2.9 Angstroms resolution Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: Arabidopsis thaliana (thale cress) |
Source (recombinant) | Organism: Homo sapiens (human) / Cell: HEK 293 |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 1600 nm / Nominal defocus min: 800 nm / Cs: 2.7 mm |
Image recording | Average exposure time: 8 sec. / Electron dose: 52 e/Å2 / Film or detector model: GATAN K2 QUANTUM (4k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 73064 / Symmetry type: POINT | ||||||||||||||||||||||||
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