[English] 日本語
Yorodumi- PDB-8om3: Small subunit of yeast mitochondrial ribosome in complex with IF3... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8om3 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Small subunit of yeast mitochondrial ribosome in complex with IF3/Aim23. | |||||||||
Components |
| |||||||||
Keywords | RIBOSOME / mitochondria / initiation factor 3 / pre-initiation complex | |||||||||
Function / homology | Function and homology information Branched-chain amino acid catabolism / 3-hydroxyisobutyryl-CoA hydrolase / mitochondrial translational initiation / 3-hydroxyisobutyryl-CoA hydrolase activity / valine catabolic process / mitochondrial ribosome binding / mitochondrial small ribosomal subunit / mitochondrial ribosome / mitochondrial translation / sporulation resulting in formation of a cellular spore ...Branched-chain amino acid catabolism / 3-hydroxyisobutyryl-CoA hydrolase / mitochondrial translational initiation / 3-hydroxyisobutyryl-CoA hydrolase activity / valine catabolic process / mitochondrial ribosome binding / mitochondrial small ribosomal subunit / mitochondrial ribosome / mitochondrial translation / sporulation resulting in formation of a cellular spore / superoxide dismutase activity / translation initiation factor activity / mRNA processing / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / mitochondrial inner membrane / rRNA binding / ribosome / structural constituent of ribosome / translation / mRNA binding / GTP binding / mitochondrion / RNA binding / ATP binding / metal ion binding Similarity search - Function | |||||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.87 Å | |||||||||
Authors | Itoh, Y. / Chicherin, I. / Kamenski, P. / Amunts, A. | |||||||||
Funding support | European Union, Sweden, 2items
| |||||||||
Citation | Journal: Mol Cell / Year: 2024 Title: METTL17 is an Fe-S cluster checkpoint for mitochondrial translation. Authors: Tslil Ast / Yuzuru Itoh / Shayan Sadre / Jason G McCoy / Gil Namkoong / Jordan C Wengrod / Ivan Chicherin / Pallavi R Joshi / Piotr Kamenski / Daniel L M Suess / Alexey Amunts / Vamsi K Mootha / Abstract: Friedreich's ataxia (FA) is a debilitating, multisystemic disease caused by the depletion of frataxin (FXN), a mitochondrial iron-sulfur (Fe-S) cluster biogenesis factor. To understand the cellular ...Friedreich's ataxia (FA) is a debilitating, multisystemic disease caused by the depletion of frataxin (FXN), a mitochondrial iron-sulfur (Fe-S) cluster biogenesis factor. To understand the cellular pathogenesis of FA, we performed quantitative proteomics in FXN-deficient human cells. Nearly every annotated Fe-S cluster-containing protein was depleted, indicating that as a rule, cluster binding confers stability to Fe-S proteins. We also observed depletion of a small mitoribosomal assembly factor METTL17 and evidence of impaired mitochondrial translation. Using comparative sequence analysis, mutagenesis, biochemistry, and cryoelectron microscopy, we show that METTL17 binds to the mitoribosomal small subunit during late assembly and harbors a previously unrecognized [FeS] cluster required for its stability. METTL17 overexpression rescued the mitochondrial translation and bioenergetic defects, but not the cellular growth, of FXN-depleted cells. These findings suggest that METTL17 acts as an Fe-S cluster checkpoint, promoting translation of Fe-S cluster-rich oxidative phosphorylation (OXPHOS) proteins only when Fe-S cofactors are replete. | |||||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 8om3.cif.gz | 3.9 MB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb8om3.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 8om3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/om/8om3 ftp://data.pdbj.org/pub/pdb/validation_reports/om/8om3 | HTTPS FTP |
---|
-Related structure data
Related structure data | 16967MC 8om2C 8om4C M: map data used to model this data C: citing same article (ref.) |
---|---|
Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
|
---|---|
1 |
|
-Components
+37S ribosomal protein ... , 28 types, 28 molecules ABDEFGHIJKLMNOPQRSTUVWYZ3456
-Protein , 4 types, 4 molecules C28d
#3: Protein | Mass: 47170.996 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: cell / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / Strain: XPM171a / References: UniProt: P02381 |
---|---|
#28: Protein | Mass: 15321.684 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: cell / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / Strain: XPM171a / References: UniProt: P38783 |
#33: Protein | Mass: 56351.195 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: cell / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / Strain: XPM171a References: UniProt: P28817, 3-hydroxyisobutyryl-CoA hydrolase |
#35: Protein | Mass: 42613.383 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Strain: XPM171a / Gene: AIM23, YJL131C, J0682 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Rosetta / References: UniProt: P47015 |
-Mitochondrial ... , 2 types, 2 molecules X1
#24: Protein | Mass: 12420.666 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: cell / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / Strain: XPM171a / References: UniProt: P53305 |
---|---|
#27: Protein | Mass: 12800.426 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: cell / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / Strain: XPM171a / References: UniProt: P32344 |
-RNA chain / Sugars , 2 types, 2 molecules r
#34: RNA chain | Mass: 528125.688 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: cell / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / Strain: XPM171a |
---|---|
#38: Sugar | ChemComp-LMT / |
-Non-polymers , 4 types, 1607 molecules
#36: Chemical | ChemComp-MG / #37: Chemical | ChemComp-K / #39: Chemical | ChemComp-ATP / | #40: Water | ChemComp-HOH / | |
---|
-Details
Has ligand of interest | Y |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Small subunit of mitochondrial ribosome in complex with IF3/Aim23 Type: RIBOSOME / Entity ID: #1-#7, #9-#35 / Source: NATURAL | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Molecular weight | Experimental value: NO | ||||||||||||||||||||||||||||||
Source (natural) | Organism: Saccharomyces cerevisiae (brewer's yeast) | ||||||||||||||||||||||||||||||
Buffer solution | pH: 7.5 | ||||||||||||||||||||||||||||||
Buffer component |
| ||||||||||||||||||||||||||||||
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | ||||||||||||||||||||||||||||||
Specimen support | Grid material: COPPER / Grid type: Quantifoil R2/1 | ||||||||||||||||||||||||||||||
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy / Nominal magnification: 165000 X / Nominal defocus max: 2600 nm / Nominal defocus min: 500 nm / Cs: 2.7 mm / C2 aperture diameter: 70 µm / Alignment procedure: COMA FREE |
Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Image recording | Electron dose: 30 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
EM imaging optics | Energyfilter name: GIF Quantum LS / Energyfilter slit width: 40 eV |
-Processing
EM software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
Symmetry | Point symmetry: C1 (asymmetric) | ||||||||||||||||||||||||
3D reconstruction | Resolution: 2.87 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 53922 / Symmetry type: POINT | ||||||||||||||||||||||||
Atomic model building | Space: REAL | ||||||||||||||||||||||||
Atomic model building | PDB-ID: 5MRC Accession code: 5MRC / Source name: PDB / Type: experimental model |