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- EMDB-16966: Small subunit of yeast mitochondrial ribosome in complex with MET... -

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Basic information

Entry
Database: EMDB / ID: EMD-16966
TitleSmall subunit of yeast mitochondrial ribosome in complex with METTL17/Rsm22.
Map dataOversampled composite map of local-mask refined maps with sharpening and local resolution filtering
Sample
  • Complex: Small subunit of mitochondrial ribosome in complex with METTL17/Rsm22
    • Protein or peptide: x 34 types
    • RNA: x 1 types
  • Ligand: x 6 types
Keywordsmitochondria / assembly / biogenesis / iron-sulfur cluster / 4Fe-4S / RIBOSOME
Function / homology
Function and homology information


Branched-chain amino acid catabolism / 3-hydroxyisobutyryl-CoA hydrolase / mitochondrial translational initiation / 3-hydroxyisobutyryl-CoA hydrolase activity / valine catabolic process / mitochondrial small ribosomal subunit / mitochondrial ribosome / mitochondrial translation / sporulation resulting in formation of a cellular spore / superoxide dismutase activity ...Branched-chain amino acid catabolism / 3-hydroxyisobutyryl-CoA hydrolase / mitochondrial translational initiation / 3-hydroxyisobutyryl-CoA hydrolase activity / valine catabolic process / mitochondrial small ribosomal subunit / mitochondrial ribosome / mitochondrial translation / sporulation resulting in formation of a cellular spore / superoxide dismutase activity / Transferases; Transferring one-carbon groups; Methyltransferases / methyltransferase activity / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / mRNA processing / ribosomal small subunit assembly / cytosolic small ribosomal subunit / small ribosomal subunit / methylation / mitochondrial inner membrane / rRNA binding / ribosome / structural constituent of ribosome / translation / mRNA binding / GTP binding / mitochondrion / RNA binding / ATP binding / metal ion binding
Similarity search - Function
Ribosomal protein S22, mitochondrial, budding yeast / Ribosomal protein VAR1 / : / Mitochondrial ribosomal protein (VAR1) / Mitochondrial ribosomal protein MRP51, fungi / Mitochondrial ribosomal protein S25 / Ribosomal protein S24, mitochondrial / Ribosomal protein S23, mitochondrial, fungi / Mitochondrial ribosomal protein subunit / Mitochondrial ribosomal protein S25 ...Ribosomal protein S22, mitochondrial, budding yeast / Ribosomal protein VAR1 / : / Mitochondrial ribosomal protein (VAR1) / Mitochondrial ribosomal protein MRP51, fungi / Mitochondrial ribosomal protein S25 / Ribosomal protein S24, mitochondrial / Ribosomal protein S23, mitochondrial, fungi / Mitochondrial ribosomal protein subunit / Mitochondrial ribosomal protein S25 / Ribosomal protein MRP10, mitochondrial / Ribosomal protein S35, mitochondrial / Ribosomal protein Rsm22-like / Mitochondrial small ribosomal subunit Rsm22 / Eukaryotic mitochondrial regulator protein / Enoyl-CoA hydratase/isomerase domain / IGR protein motif / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Enoyl-CoA hydratase/isomerase / Protein Fyv4 / IGR protein motif / IGR / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / Enoyl-CoA hydratase/isomerase, conserved site / Enoyl-CoA hydratase/isomerase signature. / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / Manganese/iron superoxide dismutase, N-terminal domain superfamily / CHCH / CHCH domain / Coiled coil-helix-coiled coil-helix (CHCH) domain profile. / ClpP/crotonase-like domain superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S15, bacterial-type / Ribosomal protein S2 signature 2. / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S14, conserved site / Ribosomal protein S15/S19, conserved site / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S10 / Ribosomal protein S14 signature. / Ribosomal protein S7, conserved site / Ribosomal protein S19 / Ribosomal protein S2 signature 1. / Ribosomal protein S13, conserved site / Ribosomal protein S2, conserved site / Ribosomal protein S13 / 30s ribosomal protein S13, C-terminal / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S14 / Ribosomal protein S2 / Ribosomal protein S4, conserved site / Ribosomal protein S13/S18 / Ribosomal protein S4/S9 / Ribosomal protein S19 signature. / Ribosomal protein S14p/S29e / Ribosomal protein S8 / Ribosomal protein S8 superfamily / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S7 signature. / Ribosomal protein S5 / Ribosomal protein S5, N-terminal / Ribosomal protein S10p/S20e / Ribosomal protein S13-like, H2TH / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, C-terminal / Ribosomal protein S9, conserved site / Ribosomal protein S5, N-terminal domain / Ribosomal protein S8 / Ribosomal protein S10 domain / Ribosomal protein S10 domain superfamily / Ribosomal protein S5, C-terminal domain / S4 RNA-binding domain
Similarity search - Domain/homology
Small ribosomal subunit protein mS37 / Small ribosomal subunit protein uS3m / Small ribosomal subunit protein mS43 / Small ribosomal subunit protein uS14m / Small ribosomal subunit protein mS27 / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein uS4m / Small ribosomal subunit protein bS6m / Small ribosomal subunit protein mS47 ...Small ribosomal subunit protein mS37 / Small ribosomal subunit protein uS3m / Small ribosomal subunit protein mS43 / Small ribosomal subunit protein uS14m / Small ribosomal subunit protein mS27 / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein uS4m / Small ribosomal subunit protein bS6m / Small ribosomal subunit protein mS47 / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein uS2m / Small ribosomal subunit protein uS5m / Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial / Small ribosomal subunit protein uS9m / Small ribosomal subunit protein bS21m / Small ribosomal subunit protein mS41 / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein mS23 / Small ribosomal subunit protein uS11m / Small ribosomal subunit protein mS42 / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein mS45 / Small ribosomal subunit protein mS33 / Small ribosomal subunit protein uS12m / Small ribosomal subunit protein uS19m / Small ribosomal subunit protein uS13m / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein bS1m / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein uS17m / Small ribosomal subunit protein uS8m / Small ribosomal subunit protein mS35
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.57 Å
AuthorsItoh Y / Chicherin I / Kamenski P / Amunts A
Funding supportEuropean Union, Sweden, 2 items
OrganizationGrant numberCountry
European Research Council (ERC)ERC-2018-StG-805230European Union
The Swedish Foundation for Strategic ResearchFFL15:0325 Sweden
CitationJournal: Mol Cell / Year: 2024
Title: METTL17 is an Fe-S cluster checkpoint for mitochondrial translation.
Authors: Tslil Ast / Yuzuru Itoh / Shayan Sadre / Jason G McCoy / Gil Namkoong / Jordan C Wengrod / Ivan Chicherin / Pallavi R Joshi / Piotr Kamenski / Daniel L M Suess / Alexey Amunts / Vamsi K Mootha /
Abstract: Friedreich's ataxia (FA) is a debilitating, multisystemic disease caused by the depletion of frataxin (FXN), a mitochondrial iron-sulfur (Fe-S) cluster biogenesis factor. To understand the cellular ...Friedreich's ataxia (FA) is a debilitating, multisystemic disease caused by the depletion of frataxin (FXN), a mitochondrial iron-sulfur (Fe-S) cluster biogenesis factor. To understand the cellular pathogenesis of FA, we performed quantitative proteomics in FXN-deficient human cells. Nearly every annotated Fe-S cluster-containing protein was depleted, indicating that as a rule, cluster binding confers stability to Fe-S proteins. We also observed depletion of a small mitoribosomal assembly factor METTL17 and evidence of impaired mitochondrial translation. Using comparative sequence analysis, mutagenesis, biochemistry, and cryoelectron microscopy, we show that METTL17 binds to the mitoribosomal small subunit during late assembly and harbors a previously unrecognized [FeS] cluster required for its stability. METTL17 overexpression rescued the mitochondrial translation and bioenergetic defects, but not the cellular growth, of FXN-depleted cells. These findings suggest that METTL17 acts as an Fe-S cluster checkpoint, promoting translation of Fe-S cluster-rich oxidative phosphorylation (OXPHOS) proteins only when Fe-S cofactors are replete.
History
DepositionMar 31, 2023-
Header (metadata) releaseJan 10, 2024-
Map releaseJan 10, 2024-
UpdateJan 31, 2024-
Current statusJan 31, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_16966.png

Downloads & links

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Map

FileDownload / File: emd_16966.map.gz / Format: CCP4 / Size: 1.1 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationOversampled composite map of local-mask refined maps with sharpening and local resolution filtering
Voxel sizeX=Y=Z: 0.59286 Å
Density
Contour LevelBy AUTHOR: 0.05
Minimum - Maximum-0.10600333 - 0.8711276
Average (Standard dev.)0.0014001911 (±0.014863718)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderYXZ
Origin000
Dimensions672672672
Spacing672672672
CellA=B=C: 398.40192 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Composite map of local-mask refined maps without sharpening

Fileemd_16966_additional_1.map
AnnotationComposite map of local-mask refined maps without sharpening
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Small subunit of mitochondrial ribosome in complex with METTL17/Rsm22

EntireName: Small subunit of mitochondrial ribosome in complex with METTL17/Rsm22
Components
  • Complex: Small subunit of mitochondrial ribosome in complex with METTL17/Rsm22
    • Protein or peptide: 37S ribosomal protein MRP51, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein MRP4, mitochondrialRibosome
    • Protein or peptide: Ribosomal protein VAR1, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein NAM9, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S5, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein MRP17, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S7, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S8, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S9, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S10, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S18, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S12, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein SWS2, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein MRP2, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S28, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S16, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S17, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein RSM18, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S19, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein MRP21, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S25, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein PET123, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S23, mitochondrialRibosome
    • Protein or peptide: Mitochondrial 37S ribosomal protein S27
    • Protein or peptide: 37S ribosomal protein S24, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein MRP10, mitochondrialRibosome
    • Protein or peptide: Mitochondrial mRNA-processing protein COX24
    • Protein or peptide: Protein FYV4, mitochondrial
    • Protein or peptide: 37S ribosomal protein S26, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein MRP1, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein MRP13, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S35, mitochondrialRibosome
    • Protein or peptide: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
    • RNA: 15S mitochondrial rRNA
    • Protein or peptide: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial
  • Ligand: MAGNESIUM ION
  • Ligand: POTASSIUM IONPotassium
  • Ligand: DODECYL-BETA-D-MALTOSIDE
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
  • Ligand: water

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Supramolecule #1: Small subunit of mitochondrial ribosome in complex with METTL17/Rsm22

SupramoleculeName: Small subunit of mitochondrial ribosome in complex with METTL17/Rsm22
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#35
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Macromolecule #1: 37S ribosomal protein MRP51, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP51, mitochondrial / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 39.507457 KDa
SequenceString: MTLAELLGRS RIAQVANNHK PLTYTGKKFH PTHQIIETKP STLYRQEWGL KSAIPSKIKS RYLVYNDLDT LERITTFEPR GGTQWNRLR FQEMGVPIVS NIGRQNPFFK YISRPEDESH AKLSLFKEMK GDTDISPAAM KKRLKKITAL IRSFQDEFKE W LVENHPDE ...String:
MTLAELLGRS RIAQVANNHK PLTYTGKKFH PTHQIIETKP STLYRQEWGL KSAIPSKIKS RYLVYNDLDT LERITTFEPR GGTQWNRLR FQEMGVPIVS NIGRQNPFFK YISRPEDESH AKLSLFKEMK GDTDISPAAM KKRLKKITAL IRSFQDEFKE W LVENHPDE LKLNSNKLED YVVKFLNKKL ETKTNKKFNT EIIGTGGLSY SLPGKLKNSP NGVIQRTVVP GRILNVVKEN ND NKWLAAI GGFVADVVFF QSPPSSFNSM GDFIRMKTFL FEILEASMEK NGSVSMHARL LEPQNDKTRE FFNKRPIYKP LTS RRARRP SVGNIQEANN LLNIIKGN

UniProtKB: Small ribosomal subunit protein bS1m

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Macromolecule #2: 37S ribosomal protein MRP4, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP4, mitochondrial / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 44.205727 KDa
SequenceString: MQRHVFARNF RRLSLLRNPS LTKRFQSSAS GAANTPNNND EVMLLQQKLL YDEIRSELKS LSQVPEDEIL PELKKSLEQD KLSDKEQQL EAELSDFFRN YALLNKLFDS KTLDGQSSTT TAAATPTKPY PNLIPSANDK PYSSQELFLR QLNHSMRTAK L GATISKVY ...String:
MQRHVFARNF RRLSLLRNPS LTKRFQSSAS GAANTPNNND EVMLLQQKLL YDEIRSELKS LSQVPEDEIL PELKKSLEQD KLSDKEQQL EAELSDFFRN YALLNKLFDS KTLDGQSSTT TAAATPTKPY PNLIPSANDK PYSSQELFLR QLNHSMRTAK L GATISKVY YPHKDIFYPP LPENITVESL MSAGVHLGQS TSLWRSSTQS YIYGEYKGIH IIDLNQTLSY LKRAAKVVEG VS ESGGIIL FLGTRQGQKR GLEEAAKKTH GYYVSTRWIP GTLTNSTEIS GIWEKQEIDS NDNPTERALS PNETSKQVKP DLL VVLNPT ENRNALLEAI KSRVPTIAII DTDSEPSLVT YPIPGNDDSL RSVNFLLGVL ARAGQRGLQN RLARNNEK

UniProtKB: Small ribosomal subunit protein uS2m

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Macromolecule #3: Ribosomal protein VAR1, mitochondrial

MacromoleculeName: Ribosomal protein VAR1, mitochondrial / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 47.170996 KDa
SequenceString: MKLKLLNMIL SMMNKTNNNN NIIINNTLDS LMNKKLLLKN MLLDMNNKKM NNMKRMLNNN NMNPAGANPV VHRIGPAGNI NNKLQHLNN MNNWNTQIYN YNKNMEIMNT MNDKLINKLL YKMMTLKLNN MNINKIIMSK TINQHSLNKL NIKFYYYNND I NNNNNNNN ...String:
MKLKLLNMIL SMMNKTNNNN NIIINNTLDS LMNKKLLLKN MLLDMNNKKM NNMKRMLNNN NMNPAGANPV VHRIGPAGNI NNKLQHLNN MNNWNTQIYN YNKNMEIMNT MNDKLINKLL YKMMTLKLNN MNINKIIMSK TINQHSLNKL NIKFYYYNND I NNNNNNNN NNYYMNMMNK LMNIMNNNMN NNLCNILSYY YKKKVTIEPI KLSYIYLNSD IFSKYISLND MDKYNNGILT NY QRMLNNI MPKLNDHNIS MNYINNINNI NNNKYNNMIN LLNNNNNINN NNNYNNNNNN YIGNINNIYN NMTIDNIPMD ILM YKYLVG WSIKFKGRLS NNNGRTSTTN LLNGTFNNKK YLWSNINNNY KLNYIPSNHN LYNNSNINKN GKYNIKVKLN FI

UniProtKB: Small ribosomal subunit protein uS3m

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Macromolecule #4: 37S ribosomal protein NAM9, mitochondrial

MacromoleculeName: 37S ribosomal protein NAM9, mitochondrial / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 56.450949 KDa
SequenceString: MPRKANLLKS LARGRVRTSF NKYNLFNLYK KGGVDLKSKS LYQQKWTAKQ ETRAYHGEHL TEKRWQTVFK PKLDSVAQLD ASLRGGEIK ETPFLLQTFA VLEKRLDFAL FRAMFASSVR QARQFILHGN VRVNGVKIKH PSYTLKPGDM FSVKPDKVLE A LGAKKPSF ...String:
MPRKANLLKS LARGRVRTSF NKYNLFNLYK KGGVDLKSKS LYQQKWTAKQ ETRAYHGEHL TEKRWQTVFK PKLDSVAQLD ASLRGGEIK ETPFLLQTFA VLEKRLDFAL FRAMFASSVR QARQFILHGN VRVNGVKIKH PSYTLKPGDM FSVKPDKVLE A LGAKKPSF QEALKIDKTQ IVLWNKYVKE AKTEPKEVWE KKLENFEKMS DSNPKKLQFQ EFLRQYNKNL ESQQYNALKG CT QEGILRK LLNVEKEIGK SNNEPLSIDE LKQGLPEIQD SQLLESLNNA YQEFFKSGEI RREIISKCQP DELISLATEM MNP NETTKK ELSDGAKSAL RSGKRIIAES VKLWTKNITD HFKTRMSDIS DGSLTFDPKW AKNLKYHDPI KLSELEGDEP KARK LINLP WQKNYVYGRQ DPKKPFFTPW KPRPFLSPFA ILPHHLEISF KTCHAVYLRD PVARPGQSEV ISPFDVPVHE RAYMY YLRN GK

UniProtKB: Small ribosomal subunit protein uS4m

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Macromolecule #5: 37S ribosomal protein S5, mitochondrial

MacromoleculeName: 37S ribosomal protein S5, mitochondrial / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 34.941102 KDa
SequenceString: MFKRQLSTSV RYLQHYDESL LSRYYPESLL KSIKLAQQTI PEDTKFRVSR NVEFAPPYLD DFTKIHPFWD YKPGMPHLHA QEENNNFSI FRWDQVQQPL PGEGNILPPG VSLPNDGGRK SKSADVAAGL HKQTGVDPDY ITRKLTMKPL VMKRVSNQTG K GKIASFYA ...String:
MFKRQLSTSV RYLQHYDESL LSRYYPESLL KSIKLAQQTI PEDTKFRVSR NVEFAPPYLD DFTKIHPFWD YKPGMPHLHA QEENNNFSI FRWDQVQQPL PGEGNILPPG VSLPNDGGRK SKSADVAAGL HKQTGVDPDY ITRKLTMKPL VMKRVSNQTG K GKIASFYA LVVVGDKNGM VGLGEGKSRE EMSKAIFKAH WDAVRNLKEI PRYENRTIYG DIDFRYHGVK LHLRSAKPGF GL RVNHVIF EICECAGIKD LSGKVYKSRN DMNIAKGTIE AFTKAQKTLD EVALGRGKKL VDVRKVYYSS

UniProtKB: Small ribosomal subunit protein uS5m

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Macromolecule #6: 37S ribosomal protein MRP17, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP17, mitochondrial / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 15.046702 KDa
SequenceString:
MLYELIGLVR ITNSNAPKLE AKELSSTIGK LIIQNRGVVR DIVPMGIRYL PKIMKKDQEK HFRAYHFLML FDSSAAVQSE ILRTLKKDP RVIRSSIVKV DLDKQLDRAS SLHRSLGKKS ILELVNEDYQ SI

UniProtKB: Small ribosomal subunit protein bS6m

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Macromolecule #7: 37S ribosomal protein S7, mitochondrial

MacromoleculeName: 37S ribosomal protein S7, mitochondrial / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 27.857141 KDa
SequenceString: MLHCARRYML VRPRLLWQSG TCVARFQSSV RTPASEPSAE KGVDEWLEAI NELREEFSAK EYLPETSLAP PGQSKVDLLQ GSQAGSKIK PTAEQLAQWE ALKSVPIPPR KNATLDHITN MIMRHGKKEK AQTILSRALY LVYCQTRQDP IQALEKSLDE L APLMMTKT ...String:
MLHCARRYML VRPRLLWQSG TCVARFQSSV RTPASEPSAE KGVDEWLEAI NELREEFSAK EYLPETSLAP PGQSKVDLLQ GSQAGSKIK PTAEQLAQWE ALKSVPIPPR KNATLDHITN MIMRHGKKEK AQTILSRALY LVYCQTRQDP IQALEKSLDE L APLMMTKT FNTGVAKASV IPVPLNKRQR NRIAWNWIVQ SANQRVSSDF AVRLGEELTA IAKGTSSAFE KRDQIHKTAI AH RAYIQLK

UniProtKB: Small ribosomal subunit protein uS7m

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Macromolecule #8: 37S ribosomal protein S8, mitochondrial

MacromoleculeName: 37S ribosomal protein S8, mitochondrial / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 17.407439 KDa
SequenceString:
(SAC)LVKLANTCA HLQNCSKVRV ALTSIPYTKL QLQFAYNLYQ QGFLSSLQKG STMGPDKDFV EVTPDNISTR RLWVGL KYR DNKPVLSSCK LISKPNSRIH LPMEDMKKLC SGVTIRNIKP LQPGELILVR AHNNIMDINE AISKKLDGEV LCRVK

UniProtKB: Small ribosomal subunit protein uS8m

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Macromolecule #9: 37S ribosomal protein S9, mitochondrial

MacromoleculeName: 37S ribosomal protein S9, mitochondrial / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 31.980258 KDa
SequenceString: MFSRLSLFRR AALAPAPMRM SFRTIYQKTE DELPRRIVPK LATFYSANPN HEDRINRLER LLRKYIKLPS QNNNEAQQTK APWISFDEY ALIGGGTKLK PTQYTQLLYM LNKLHNIDPQ LTNDEITSEL SQYYKKSSML SNNIKIKTLD EFGRSIAVGK R KSSTAKVF ...String:
MFSRLSLFRR AALAPAPMRM SFRTIYQKTE DELPRRIVPK LATFYSANPN HEDRINRLER LLRKYIKLPS QNNNEAQQTK APWISFDEY ALIGGGTKLK PTQYTQLLYM LNKLHNIDPQ LTNDEITSEL SQYYKKSSML SNNIKIKTLD EFGRSIAVGK R KSSTAKVF VVRGTGEILV NGRQLNDYFL KMKDRESIMY PLQVIESVGK YNIFATTSGG GPTGQAESIM HAIAKALVVF NP LLKSRLH KAGVLTRDYR HVERKKPGKK KARKMPTWVK R

UniProtKB: Small ribosomal subunit protein uS9m

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Macromolecule #10: 37S ribosomal protein S10, mitochondrial

MacromoleculeName: 37S ribosomal protein S10, mitochondrial / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 23.45892 KDa
SequenceString: MLRNTIALRS FIRTQSTRPY PVNVEAVYYA PLKLPIKYGD LVADIQLRSY DNENLDFYSD FILRTGYYLG IPLTGPKPLP TRRERWTVI KSPFVHAKSK ENFERHTHKR LIRAWDTNPE VLQMLIAYIT KHSMAGVGMK CNFFQRSEIS LDLGSDANGL E KSLSNIDE ...String:
MLRNTIALRS FIRTQSTRPY PVNVEAVYYA PLKLPIKYGD LVADIQLRSY DNENLDFYSD FILRTGYYLG IPLTGPKPLP TRRERWTVI KSPFVHAKSK ENFERHTHKR LIRAWDTNPE VLQMLIAYIT KHSMAGVGMK CNFFQRSEIS LDLGSDANGL E KSLSNIDE LYSLRNDDKA QTSAVGQKVL ELLDSPDFKK HLEKK

UniProtKB: Small ribosomal subunit protein uS10m

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Macromolecule #11: 37S ribosomal protein S18, mitochondrial

MacromoleculeName: 37S ribosomal protein S18, mitochondrial / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 24.60342 KDa
SequenceString: MLLQPVWKGC RWTQFVRPIR RWNSTGTNRG VPFSFKDISN QEDITNISYP SSSDSVLTKS NGSSEVYKPK EEVVKYILHG KFTKNNTHL TFSSVVEDKN FHKNKGLTYN DTMLYYLNLP QKVKISLSTG CLGFRKAARG EYEAAFQTSG RMFELIKEKN M LNKDIEVV ...String:
MLLQPVWKGC RWTQFVRPIR RWNSTGTNRG VPFSFKDISN QEDITNISYP SSSDSVLTKS NGSSEVYKPK EEVVKYILHG KFTKNNTHL TFSSVVEDKN FHKNKGLTYN DTMLYYLNLP QKVKISLSTG CLGFRKAARG EYEAAFQTSG RMFELIKEKN M LNKDIEVV MDDFGKGRAA FISALVGKEG ASVVKKVVKI SDATKLKFGG VRSPKMRRL

UniProtKB: Small ribosomal subunit protein uS11m

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Macromolecule #12: 37S ribosomal protein S12, mitochondrial

MacromoleculeName: 37S ribosomal protein S12, mitochondrial / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 16.94874 KDa
SequenceString:
MLSRFMSNTW CTPLRQAQRL FSSTTTMQAT LNQIKRGSGP PRRKKISTAP QLDQCPQRKG VVLRVMVLKP KKPNSAQRKA CRVRLTNGN VVSAYIPGEG HDAQEHSIVY VRGGRCQDLP GVKYHVIRGA GDLSGVVNRI SSRSKYGAKK PSKS

UniProtKB: Small ribosomal subunit protein uS12m

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Macromolecule #13: 37S ribosomal protein SWS2, mitochondrial

MacromoleculeName: 37S ribosomal protein SWS2, mitochondrial / type: protein_or_peptide / ID: 13 / Details: F41L variant / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 16.087031 KDa
SequenceString:
MVVHILGKGF KGKEVIKIAL ASKFYGIGKT TAEKICSKLG LYPWMRMHQL SEPQIMSIAS ELSTMTIEGD ARAIVKDNIA LKRKIGSYS GMRHTLHLPV RGQHTRNNAK TARKLNKIDR RGIHTFSQAK VQHNPSLWSC IFGK

UniProtKB: Small ribosomal subunit protein uS13m

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Macromolecule #14: 37S ribosomal protein MRP2, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP2, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 13.563061 KDa
SequenceString:
MGNFRFPIKT KLPPGFINAR ILRDNFKRQQ FKENEILVKS LKFIARNMNL PTKLRLEAQL KLNALPNYMR STQIKNRCVD SGHARFVLS DFRLCRYQFR ENALKGNLPG VKKGIW

UniProtKB: Small ribosomal subunit protein uS14m

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Macromolecule #15: 37S ribosomal protein S28, mitochondrial

MacromoleculeName: 37S ribosomal protein S28, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 33.116383 KDa
SequenceString: MSIVGRNAIL NLRISLCPLF MGKRSFVSSP VSNSAKAVKF LKAQRRKQKN EAKQATLKAS TDKVDPVLGR ADTPFITRIM AELKEPLVL SKGYNIEEVD KFLAAIESAK RERAELSGLN TEVVGIEDIE KLEDRREAIL RILSMRNSEN KNAIKMAVEL A RKEFERFP ...String:
MSIVGRNAIL NLRISLCPLF MGKRSFVSSP VSNSAKAVKF LKAQRRKQKN EAKQATLKAS TDKVDPVLGR ADTPFITRIM AELKEPLVL SKGYNIEEVD KFLAAIESAK RERAELSGLN TEVVGIEDIE KLEDRREAIL RILSMRNSEN KNAIKMAVEL A RKEFERFP GDTGSSEVQA ACMTVRIQNM ANHIKEHRKD FANTRNLRIL VQQRQAILRY LKRDNPEKYY WTIQKLGLND AA ITDEFNM DRRYMQDYEF FGDKILIRDS KKVANQKRKE IRKQKRATF

UniProtKB: Small ribosomal subunit protein uS15m

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Macromolecule #16: 37S ribosomal protein S16, mitochondrial

MacromoleculeName: 37S ribosomal protein S16, mitochondrial / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 13.663011 KDa
SequenceString:
MTCGLVRIRL ARFGRKNSPV YNIVVANSRK ARDAKPIEVL GTYVPVPSPV TKRELKRGVV PIKDVKLDFD RTKYWIGVGA QPSETVTKL LRKAGILNDA WATSKNSNVN RKVVFERMET LE

UniProtKB: Small ribosomal subunit protein bS16m

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Macromolecule #17: 37S ribosomal protein S17, mitochondrial

MacromoleculeName: 37S ribosomal protein S17, mitochondrial / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 27.683986 KDa
SequenceString: MARQNFLGLV VSQGKMQKTV KVRVETKVFN KKINKELFHR RDYLVHDEGE ISREGDLVRI EATRPLSKRK FFAIAEIIRN KGQQFALYE SEAQLSVAKE EAQKAKEFLD KRSVRENKLN EKTTLLRDIR TIQDALSSGS TPKELLEIKQ RYGIQDFSQE T VRQLLQLD ...String:
MARQNFLGLV VSQGKMQKTV KVRVETKVFN KKINKELFHR RDYLVHDEGE ISREGDLVRI EATRPLSKRK FFAIAEIIRN KGQQFALYE SEAQLSVAKE EAQKAKEFLD KRSVRENKLN EKTTLLRDIR TIQDALSSGS TPKELLEIKQ RYGIQDFSQE T VRQLLQLD ISGLEVNLEK QRSLIDRIQT RLSELLSNDL KCDQFLKDHG VEDPLTLKKN IKKNLLRKHV MMDMQQPSQ

UniProtKB: Small ribosomal subunit protein uS17m

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Macromolecule #18: 37S ribosomal protein RSM18, mitochondrial

MacromoleculeName: 37S ribosomal protein RSM18, mitochondrial / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 15.86649 KDa
SequenceString:
MQPIIKGAVS STFKRALYNF GIKEKKSVNI EMGRTQQTKK IDQSLSKKLP KGTIYDPFDF SMGRIHLDRK YQANKNSNRN DIMKSGANP LEFYARPRIL SRYVTSTGRI QHRDITGLSA KNQRRLSKAI RRCQAIGLM

UniProtKB: Small ribosomal subunit protein bS18m

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Macromolecule #19: 37S ribosomal protein S19, mitochondrial

MacromoleculeName: 37S ribosomal protein S19, mitochondrial / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 10.292044 KDa
SequenceString:
MQPAARLLSR SVWKGPNIVP LPIREAMTKG TPIRTNARAA TILPQFVGLK FQIHNGKEYV PIEISEDMVG HKLGEFAPTR KRFSYTQTK NK

UniProtKB: Small ribosomal subunit protein uS19m

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Macromolecule #20: 37S ribosomal protein MRP21, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP21, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 20.432744 KDa
SequenceString:
MLKSTLRLSR ISLRRGFTTI DCLRQQNSDI DKIILNPIKL AQGSNSDRGQ TSKSKTDNAD ILSMEIPVDM MQSAGRINKR ELLSEAEIA RSSVENAQMR FNSGKSIIVN KNNPAESFKR LNRIMFENNI PGDKRSQRFY MKPGKVAELK RSQRHRKEFM M GFKRLIEI VKDAKRKGY

UniProtKB: Small ribosomal subunit protein bS21m

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Macromolecule #21: 37S ribosomal protein S25, mitochondrial

MacromoleculeName: 37S ribosomal protein S25, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 30.554354 KDa
SequenceString: MKIQTNAVNV LQRTSAYLKS GLLKETPAWY NVVASIPPST KFTREPRFKN PSNGHIIGKL VDVTEQPHAN NKGLYKTRPN SSDKRVGVK RLYRPPKLTY VEDRLRSLFY KQHPWELSRP KILVENEIGD ENYDWSHMLQ IGRPLDGESV IQRTMYLIKT K QYGDMVEA ...String:
MKIQTNAVNV LQRTSAYLKS GLLKETPAWY NVVASIPPST KFTREPRFKN PSNGHIIGKL VDVTEQPHAN NKGLYKTRPN SSDKRVGVK RLYRPPKLTY VEDRLRSLFY KQHPWELSRP KILVENEIGD ENYDWSHMLQ IGRPLDGESV IQRTMYLIKT K QYGDMVEA YDHARYEFYA LRMQEETEQQ VALEEAEMFG SLFGVSAIEH GIQKEQEVLD VWEKKVVEET ELMAARTSNP AG SWKDDTT LNTAQEEEST TSENLHF

UniProtKB: Small ribosomal subunit protein mS23

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Macromolecule #22: 37S ribosomal protein PET123, mitochondrial

MacromoleculeName: 37S ribosomal protein PET123, mitochondrial / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 36.059281 KDa
SequenceString: MGKGAAKYGF KSGVFPTTRS ILKSPTTKQT DIINKVKSPK PKGVLGIGYA KGVKHPKGSH RLSPKVNFID VDNLIAKTVA EPQSIKSSN GSAQKVRLQK AELRRKFLIE AFRKEEARLL HKHEYLQKRT KELEKAKELE LEKLNKEKSS DLTIMTLDKM M SQPLLRNR ...String:
MGKGAAKYGF KSGVFPTTRS ILKSPTTKQT DIINKVKSPK PKGVLGIGYA KGVKHPKGSH RLSPKVNFID VDNLIAKTVA EPQSIKSSN GSAQKVRLQK AELRRKFLIE AFRKEEARLL HKHEYLQKRT KELEKAKELE LEKLNKEKSS DLTIMTLDKM M SQPLLRNR SPEESELLKL KRNYNRSLLN FQAHKKKLNE LLNLYHVANE FIVTESQLLK KIDKVFNDET EEFTDAYDVT SN FTQFGNR KLLLSGNTTL QTQINNAIMG SLSNEKFFDI SLVDSYLNKD LKNISNKIDS KLNPTSNGAG NNGNNNNTTN L

UniProtKB: Small ribosomal subunit protein mS26

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Macromolecule #23: 37S ribosomal protein S23, mitochondrial

MacromoleculeName: 37S ribosomal protein S23, mitochondrial / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 50.940742 KDa
SequenceString: MLRMSTSRFI GQRLFTTARS LQAAKPAPKG KTQGFSKKSS SVSSYSSAKR VTPGSLYKNW TNTTHTAQLQ QTAVPLALPI FNFDDISKT LNKVVSYSNK QYKSLHHLGS FKKSQFNELF QKPVCLVRED ATNSFLKKLV SHPVKKFIIT GEPGVGKTVL L SQAHAYAV ...String:
MLRMSTSRFI GQRLFTTARS LQAAKPAPKG KTQGFSKKSS SVSSYSSAKR VTPGSLYKNW TNTTHTAQLQ QTAVPLALPI FNFDDISKT LNKVVSYSNK QYKSLHHLGS FKKSQFNELF QKPVCLVRED ATNSFLKKLV SHPVKKFIIT GEPGVGKTVL L SQAHAYAV DSKQIIINIS YPELFLNGRN DFSYDDDLKL FIQPMYLKKL IRKILKANDP ALLKSIELSK DYKFSNANPK NA SVKPFVT LNKTKNTVLD LLSVMTHPHN RGKLMKAIID ELSVQSKVPI MFTVDNFSKV LTTAYSAYRN TENKQIYSLD LQM GKLMMD IISGETKFAN GESSTILAIS GVDRTNKTLP VALGKIPVDP YVTRYHYEPK FVELLQKGNV TEFEVPKLNK QEVN ELIDY YKQSNVLLDK DITGKKWENL IDEKYFLSGN GNPRELLKSL VLSHR

UniProtKB: Small ribosomal subunit protein mS29

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Macromolecule #24: Mitochondrial 37S ribosomal protein S27

MacromoleculeName: Mitochondrial 37S ribosomal protein S27 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 12.420666 KDa
SequenceString:
MNVPKARLLK VAELSAKIFD QNFNPSGIRT GSKILNERLK GPSVASYYGN PDILKFRHLK TLYPDIEFVD LEEQYRLSMV EAKKRRGKG APKKMKKDAA ATAKGKGKKK K

UniProtKB: Small ribosomal subunit protein mS33

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Macromolecule #25: 37S ribosomal protein S24, mitochondrial

MacromoleculeName: 37S ribosomal protein S24, mitochondrial / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 37.458496 KDa
SequenceString: MKVPLGLWKV SRGNLWSTQK RVLTMSRCLN SDAGNEAKTV REGPAFSADL YMHPEKWKGL PPQRILELYW ERMARLGSEY KPNKDELNA LLTTSEYSNV PVNDIKKLYH RGEQGAIDIK GGNVNRDNSL RPFMFDELPS QAQELVAQHR EQRFYNRLAA Y ELPLLAQY ...String:
MKVPLGLWKV SRGNLWSTQK RVLTMSRCLN SDAGNEAKTV REGPAFSADL YMHPEKWKGL PPQRILELYW ERMARLGSEY KPNKDELNA LLTTSEYSNV PVNDIKKLYH RGEQGAIDIK GGNVNRDNSL RPFMFDELPS QAQELVAQHR EQRFYNRLAA Y ELPLLAQY RQEYKRPSPE SHPVTYRYTS YVGEEHPNSR KVVLSVKTKE LGLEEKSLHK FRILARSRYD HTTDIFKMSS DK FEHASQN ARYLHDILQR LLAESKDLTE DDFSDVPLDT RHTIAKSLRK KKRDYEFPEH WKRPEDAPKK KFDIVDQLLS TL

UniProtKB: Small ribosomal subunit protein mS35

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Macromolecule #26: 37S ribosomal protein MRP10, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP10, mitochondrial / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 10.709598 KDa
SequenceString:
MSGKPPVYRL PPLPRLKVKK PIIRQEANKC LVLMSNLLQC WSSYGHMSPK CAGLVTELKS CTSESALGKR NNVQKSNINY HAARLYDRI NGKPHD

UniProtKB: Small ribosomal subunit protein mS37

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Macromolecule #27: Mitochondrial mRNA-processing protein COX24

MacromoleculeName: Mitochondrial mRNA-processing protein COX24 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 12.800426 KDa
SequenceString:
MLGRALRPGW LGITRTVVKK PSCGSYFNRT FQTAINTTMP PMQEGMLSTM MMMTATATRI TGTVSEPLNG SNIVMQLDSV MRKRKKKMK KHKLRKRRKR EKAERRKLSQ GR

UniProtKB: Small ribosomal subunit protein mS38

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Macromolecule #28: Protein FYV4, mitochondrial

MacromoleculeName: Protein FYV4, mitochondrial / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 15.321684 KDa
SequenceString:
MIPSRISHKF PLFLRSSLAA PKAAYRFSST IPKPSDQVPD VDAFLNKIGR NCNELKDTFE NNWNNLFQWD SKILKEKGVN IQQRKYILK QVHNYRNNRP IHEIKLGKKS FFGGERKRKA FTAKWKAENK Q

UniProtKB: Small ribosomal subunit protein mS41

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Macromolecule #29: 37S ribosomal protein S26, mitochondrial

MacromoleculeName: 37S ribosomal protein S26, mitochondrial / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 30.257449 KDa
SequenceString: MLVFKRGIHV VPKLPNSKAL LQNGVPNILS SSGFKTVWFD YQRYLCDKLT LATAGQSLES YYPFHILLKT AGNPLQSNIF NLASSIHNN HLFVENILPS AVEHGTNSNA VVKTEPSRLF LSKIKDSFNG SDWEVVKEEM IYRAENEVLG QGWLFLVENN E KKLFILTS ...String:
MLVFKRGIHV VPKLPNSKAL LQNGVPNILS SSGFKTVWFD YQRYLCDKLT LATAGQSLES YYPFHILLKT AGNPLQSNIF NLASSIHNN HLFVENILPS AVEHGTNSNA VVKTEPSRLF LSKIKDSFNG SDWEVVKEEM IYRAENEVLG QGWLFLVENN E KKLFILTS NNNGTPYYFP RNQSFDLNSA ISIDEFATLK QMKELIGKST KLNGKVQDWT MPIICVNLWD HAYLHDYGVG NR SKYVKNV LDNLNWSVVN NRIFSGISK

UniProtKB: Small ribosomal subunit protein mS42

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Macromolecule #30: 37S ribosomal protein MRP1, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP1, mitochondrial / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 36.775781 KDa
SequenceString: MLRFTGARAI RKYSTRYALE HLKEGAPLKG LFSIEGLQKA WFDRVKYLDA KLNDCTNEAQ QKPLETLIHE NSKSASKKHI VNYASSLYN LKFSMSSLQG CIRTPPEECP RLGPEALLQT PDFNRTISNE PLTTGNERLQ AALISSFGSL MEFRTLLINS N LAISGDGF ...String:
MLRFTGARAI RKYSTRYALE HLKEGAPLKG LFSIEGLQKA WFDRVKYLDA KLNDCTNEAQ QKPLETLIHE NSKSASKKHI VNYASSLYN LKFSMSSLQG CIRTPPEECP RLGPEALLQT PDFNRTISNE PLTTGNERLQ AALISSFGSL MEFRTLLINS N LAISGDGF TWLVARRQLD KRAMRNDMPN RDIEYDKLFI LNTYNAGTPF NFSTSGVMNE LNNQYTNMEK QRAKEAGNLE DS EMTAKQA KTKFIYETQQ KGFSGKEVSY IPLLAIDASP KTWLTDYGVF GKREYLERVW DSIEWKIVES RLPQRTKIQA FNT L

UniProtKB: Small ribosomal subunit protein mS43

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Macromolecule #31: 37S ribosomal protein MRP13, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP13, mitochondrial / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 39.045031 KDa
SequenceString: MGTITVVINE GPILLIRALH RATTNKKMFR STVWRRFAST GEIAKAKLDE FLIYHKTDAK LKPFIYRPKN AQILLTKDIR DPKTREPLQ PRPPVKPLSK QTLNDFIYSV EPNSTELLDW FKEWTGTSIR KRAIWTYISP IHVQKMLTAS FFKIGKYAHM V GLLYGIEH ...String:
MGTITVVINE GPILLIRALH RATTNKKMFR STVWRRFAST GEIAKAKLDE FLIYHKTDAK LKPFIYRPKN AQILLTKDIR DPKTREPLQ PRPPVKPLSK QTLNDFIYSV EPNSTELLDW FKEWTGTSIR KRAIWTYISP IHVQKMLTAS FFKIGKYAHM V GLLYGIEH KFLKAQNPSV FDIEHFFNTN IMCALHRNRL KDYKDAEIAQ RKLQVAWKKV LNRKNNTGLA NILVATLGRQ IG FTPELTG LQPVDISLPD IPNSSSGAEL KDLLSKYEGI YLIARTLLDI DQHNAQYLEL QEFIRQYQNA LSESSDPYDT HLK ALGLLE TPPPQESTEK EEK

UniProtKB: Small ribosomal subunit protein mS27

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Macromolecule #32: 37S ribosomal protein S35, mitochondrial

MacromoleculeName: 37S ribosomal protein S35, mitochondrial / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 39.638172 KDa
SequenceString: MSYGLTGTSS KLRGTSSIFS WTQVRHFSRR RIAYPFYPFK KLGRQHPKKH DTNLKTAMRQ FLGPKNYKGE YVMNKYFTVP TNHVPNYIK PDLERGQSLE HPVTKKPLQL RYDGTLGPPP VENKRLQNIF KDRLLQPFPS NPHCKTNYVL SPQLKQSIFE E ITVEGLSA ...String:
MSYGLTGTSS KLRGTSSIFS WTQVRHFSRR RIAYPFYPFK KLGRQHPKKH DTNLKTAMRQ FLGPKNYKGE YVMNKYFTVP TNHVPNYIK PDLERGQSLE HPVTKKPLQL RYDGTLGPPP VENKRLQNIF KDRLLQPFPS NPHCKTNYVL SPQLKQSIFE E ITVEGLSA QQVSQKYGLK IPRVEAIVKL VSVENSWNRR NRVSSDLKTM DETLYRMFPV FDSDASFKRE NLSEIPVPQK TL ASRFLTI AESEPFGPVD AAHVLELEPA VETLRNLSTV GEHSSGHQQS TNKNTKVIYG ELVEGERSQY KFTNAKVGKV GYR YGSGNR DNKKDRRIGF NKLGQMVYI

UniProtKB: Small ribosomal subunit protein mS45

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Macromolecule #33: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial

MacromoleculeName: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO / EC number: 3-hydroxyisobutyryl-CoA hydrolase
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 56.351195 KDa
SequenceString: MLRNTLKCAQ LSSKYGFKTT TRTFMTTQPQ LNVTDAPPVL FTVQDTARVI TLNRPKKLNA LNAEMSESMF KTLNEYAKSD TTNLVILKS SNRPRSFCAG GDVATVAIFN FNKEFAKSIK FFTDEYSLNF QIATYLKPIV TFMDGITMGG GVGLSIHTPF R IATENTKW ...String:
MLRNTLKCAQ LSSKYGFKTT TRTFMTTQPQ LNVTDAPPVL FTVQDTARVI TLNRPKKLNA LNAEMSESMF KTLNEYAKSD TTNLVILKS SNRPRSFCAG GDVATVAIFN FNKEFAKSIK FFTDEYSLNF QIATYLKPIV TFMDGITMGG GVGLSIHTPF R IATENTKW AMPEMDIGFF PDVGSTFALP RIVTLANSNS QMALYLCLTG EVVTGADAYM LGLASHYVSS ENLDALQKRL GE ISPPFNN DPQSAYFFGM VNESIDEFVS PLPKDYVFKY SNEKLNVIEA CFNLSKNGTI EDIMNNLRQY EGSAEGKAFA QEI KTKLLT KSPSSLQIAL RLVQENSRDH IESAIKRDLY TAANMCMNQD SLVEFSEATK HKLIDKQRVP YPWTKKEQLF VSQL TSITS PKPSLPMSLL RNTSNVTWTQ YPYHSKYQLP TEQEIAAYIE KRTNDDTGAK VTEREVLNHF ANVIPSRRGK LGIQS LCKI VCERKCEEVN DGLRWK

UniProtKB: Small ribosomal subunit protein mS47

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Macromolecule #35: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase ...

MacromoleculeName: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial
type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
EC number: Transferases; Transferring one-carbon groups; Methyltransferases
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 72.308328 KDa
SequenceString: MMKRCFSILP QNVRFSSKFT SLNLPKLDLA DFIDSNKRGI NVLPSYRDET ASTTQATNSK ELRLLSKTLQ GQSYRDQLEL NPDVSKAIN NNIMAVHIPN NLRRVATNYY KEIQEPNSLH RPCRTKMEVD AHIASIFLQN YGSIFQSLKE LQKRVGPDNF K PQRILDVG ...String:
MMKRCFSILP QNVRFSSKFT SLNLPKLDLA DFIDSNKRGI NVLPSYRDET ASTTQATNSK ELRLLSKTLQ GQSYRDQLEL NPDVSKAIN NNIMAVHIPN NLRRVATNYY KEIQEPNSLH RPCRTKMEVD AHIASIFLQN YGSIFQSLKE LQKRVGPDNF K PQRILDVG YGPATGIVAL NDILGPNYRP DLKDAVILGN AEMQERAKII LSRQLNEVVD TVEENVSTEK EQETDRRNKN FQ EDEHIGE VMTKKINIMT NLRSSIPASK EYDLIILTHQ LLHDGNQFPI QVDENIEHYL NILAPGGHIV IIERGNPMGF EII ARARQI TLRPENFPDE FGKIPRPWSR GVTVRGKKDA ELGNISSNYF LKVIAPCPHQ RKCPLQVGNP NFYTHKEGKD LKFC NFQKS IKRPKFSIEL KKGKLLATSW DGSQGNASRL KGTGRRNGRD YEILNYSYLI FERSHKDENT LKEIKKLRNE NVNGK YDIG SLGDDTQNSW PRIINDPVKR KGHVMMDLCA PSGELEKWTV SRSFSKQIYH DARKSKKGDL WASAAKTQIK GLGDLN VKK FHKLEKERIK QLKKEERQKA RKAMESYNEL EDSLQFDDHQ FSNFEVMKKL STFHGNDFLQ HVNRK

UniProtKB: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial

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Macromolecule #34: 15S mitochondrial rRNA

MacromoleculeName: 15S mitochondrial rRNA / type: rna / ID: 34 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 528.125688 KDa
SequenceString: GUAAAAAAUU UAUAAGAAUA UGAUGUUGGU UCAGAUUAAG CGCUAAAUAA GGACAUGACA CAUGCGAAUC AUACGUUUAU UAUUGAUAA GAUAAUAAAU AUGUGGUGUA AACGUGAGUA AUUUUAUUAG GAAUUAAUGA ACUAUAGAAU AAGCUAAAUA C UUAAUAUA ...String:
GUAAAAAAUU UAUAAGAAUA UGAUGUUGGU UCAGAUUAAG CGCUAAAUAA GGACAUGACA CAUGCGAAUC AUACGUUUAU UAUUGAUAA GAUAAUAAAU AUGUGGUGUA AACGUGAGUA AUUUUAUUAG GAAUUAAUGA ACUAUAGAAU AAGCUAAAUA C UUAAUAUA UUAUUAUAUA AAAAUAAUUU AUAUAAUAAA AAGGAUAUAU AUAUAAUAUA UAUUUAUCUA UAGUCAAGCC AA UAAUGGU UUAGGUAGUA GGUUUAUUAA GAGUUAAACC UAGCCAACGA UCCAUAAUCG AUAAUGAAAG UUAGAACGAU CAC GUUGAC UCUGAAAUAU AGUCAAUAUC UAUAAGAUAC AGCAGUGAGG AAUAUUGGAC AAUGAUCGAA AGAUUGAUCC AGUU ACUUA UUAGGAUGAU AUAUAAAAAU AUUUUAUUUU AUUUAUAAAU AUUAAAUAUU UAUAAUAAUA AUAAUAAUAA UAUAU AUAU AUAAAUUGAU UAAAAAUAAA AUCCAUAAAU AAUUAAAAUA AUGAUAUUAA UUACCAUAUA UAUUUUUAUA UGGAUA UAU AUAUUAAUAA UAAUAUUAAU UUUAUUAUUA UUAAUAAUAU AUUUUAAUAG UCCUGACUAA UAUUUGUGCC AGCAGUC GC GGUAACACAA AGAGGGCGAG CGUUAAUCAU AAUGGUUUAA AGGAUCCGUA GAAUGAAUUA UAUAUUAUAA UUUAGAGU U AAUAAAAUUA AUUAAAGAAU UAUAAUAGUA AAGAUGAAAU AAUAAUAAUA AUUAUAAGAC UAAUAUAUGU GAAAAUAUU AAUUAAAUAU UAACUGACAU UGAGGGAUUA AAACUAGAGU AGCGAAACGG AUUCGAUACC CGUGUAGUUC UAGUAGUAAA CUAUGAAUA CAAUUAUUUA UAUAUAUAUU AUAUAUAAAU AAUAAAUGAA AAUGAAAGUA UUCCACCUGA AGAGUACGUU A GCAAUAAU GAAACUCAAA ACAAUAGACG GUUACAGACU UAAGCAGUGG AGCAUGUUAU UUAAUUCGAU AAUCCACGAC UA ACCUUAC CAUAUUUUGA AUAUUAUAAU AAUUAUUAUA AUUAUUAUAU UACAGGCGUU ACAUUGUUGU CUUUAGUUCG UGC UGCAAA GUUUUAGAUU AAGUUCAUAA ACGAACAAAA CUCCAUAUAU AUAAUUUUAA UUAUAUAUAA UUUUAUAUUA UUUA UUAAU AUAAAGAAAG GAAUUAAGAC AAAUCAUAAU GAUCCUUAUA AUAUGGGUAA UAGACGUGCU AUAAUAAAAU GAUAA UAAA AUUAUAUAAA AUAUAUUUAA UUAUAUUUAA UUAAUAAUAU AAAACAUUUU AAUUUUUAAU AUAUUUUUUU AUUAUA UAU UAAUAUGAAU UAUAAUCUGA AAUUCGAUUA UAUGAAAAAA GAAUUGCUAG UAAUACGUAA AUUAGUAUGU UACGGUG AA UAUUCUAACU GUUUCGCACU AAUCACUCAU CACGCGUUGA AACAUAUUAU UAUCUUAUUA UUUAUAUAAU AUUUUUUA A UAAAUAUUAA UAAUUAUUAA UUUAUAUUUA UUUAUAUCAG AAAUAAUAUG AAUUAAUGCG AAGUUGAAAU ACAGUUACC GUAGGGGAAC CUGCGGUGGG CUUAUAAAUA UCUUAAAUAU UCUUACA

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Macromolecule #36: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 36 / Number of copies: 90 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #37: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 37 / Number of copies: 35 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #38: DODECYL-BETA-D-MALTOSIDE

MacromoleculeName: DODECYL-BETA-D-MALTOSIDE / type: ligand / ID: 38 / Number of copies: 1 / Formula: LMT
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

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Macromolecule #39: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 39 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

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Macromolecule #40: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 40 / Number of copies: 1 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

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Macromolecule #41: water

MacromoleculeName: water / type: ligand / ID: 41 / Number of copies: 3014 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Component:
ConcentrationFormulaName
50.0 mMKClpotassium chloride
20.0 mMHEPES-KOH buffer pH7.5
5.0 mMmagnesium acetate
2.0 mMdithiothreitol
0.05 %n-dodecyl-beta-D-maltoside
GridModel: Quantifoil R2/1 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 3 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 2.8000000000000003 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 165000
Specialist opticsEnergy filter - Name: GIF Quantum LS / Energy filter - Slit width: 40 eV
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 32.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: EMDB MAP
EMDB ID:

3551

Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.57 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 283744

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Atomic model buiding 1

Initial modelPDB ID:

5mrc


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: REAL
Output model

PDB-8om2:
Small subunit of yeast mitochondrial ribosome in complex with METTL17/Rsm22.

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