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- PDB-8ee8: Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ10... -

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Basic information

Entry
Database: PDB / ID: 8ee8
TitleCrystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ100-C in complex with ZIKV E glycoprotein
Components
  • Envelope protein E
  • rhMZ100-C antibody heavy chain
  • rhMZ100-C antibody light chain
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / ZIKV / ZIKV-specific / cross-protomer epitopes / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex
Function / homology
Function and homology information


symbiont-mediated suppression of host JAK-STAT cascade via inhibition of host TYK2 activity / flavivirin / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT2 activity / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / negative regulation of innate immune response / viral capsid / nucleoside-triphosphate phosphatase / double-stranded RNA binding / 4 iron, 4 sulfur cluster binding / mRNA (guanine-N7)-methyltransferase ...symbiont-mediated suppression of host JAK-STAT cascade via inhibition of host TYK2 activity / flavivirin / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT2 activity / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / negative regulation of innate immune response / viral capsid / nucleoside-triphosphate phosphatase / double-stranded RNA binding / 4 iron, 4 sulfur cluster binding / mRNA (guanine-N7)-methyltransferase / methyltransferase cap1 / clathrin-dependent endocytosis of virus by host cell / mRNA (nucleoside-2'-O-)-methyltransferase activity / mRNA 5'-cap (guanine-N7-)-methyltransferase activity / RNA helicase activity / molecular adaptor activity / host cell endoplasmic reticulum membrane / host cell perinuclear region of cytoplasm / protein dimerization activity / RNA helicase / induction by virus of host autophagy / RNA-directed RNA polymerase / viral RNA genome replication / RNA-dependent RNA polymerase activity / serine-type endopeptidase activity / centrosome / fusion of virus membrane with host endosome membrane / viral envelope / lipid binding / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / host cell nucleus / virion attachment to host cell / GTP binding / virion membrane / structural molecule activity / ATP hydrolysis activity / proteolysis / extracellular region / ATP binding / membrane / metal ion binding
Similarity search - Function
: / Flavivirus envelope glycoprotein E, stem/anchor domain / RNA-directed RNA polymerase, thumb domain, Flavivirus / Flavivirus RNA-directed RNA polymerase, thumb domain / Flavivirus capsid protein C superfamily / : / Flavivirus non-structural protein NS2B / Flavivirus NS3 helicase, C-terminal helical domain / Genome polyprotein, Flavivirus / Flavivirus non-structural protein NS4A ...: / Flavivirus envelope glycoprotein E, stem/anchor domain / RNA-directed RNA polymerase, thumb domain, Flavivirus / Flavivirus RNA-directed RNA polymerase, thumb domain / Flavivirus capsid protein C superfamily / : / Flavivirus non-structural protein NS2B / Flavivirus NS3 helicase, C-terminal helical domain / Genome polyprotein, Flavivirus / Flavivirus non-structural protein NS4A / Flavivirus non-structural protein NS2B / Flavivirus capsid protein C / Flavivirus non-structural protein NS4B / mRNA cap 0/1 methyltransferase / Flavivirus capsid protein C / Flavivirus non-structural protein NS4B / Flavivirus non-structural protein NS4A / Flavivirus NS2B domain profile. / mRNA cap 0 and cap 1 methyltransferase (EC 2.1.1.56 and EC 2.1.1.57) domain profile. / Flavivirus non-structural protein NS2A / Flavivirus non-structural protein NS2A / Flavivirus NS3, petidase S7 / Peptidase S7, Flavivirus NS3 serine protease / Flavivirus NS3 protease (NS3pro) domain profile. / Envelope glycoprotein M, flavivirus / Flavivirus envelope glycoprotein M / RNA-directed RNA polymerase, flavivirus / Flavivirus RNA-directed RNA polymerase, fingers and palm domains / Flavivirus non-structural Protein NS1 / Flavivirus non-structural protein NS1 / Envelope glycoprotein M superfamily, flavivirus / Flavivirus polyprotein propeptide / Flavivirus polyprotein propeptide superfamily / Flavivirus polyprotein propeptide / Flaviviral glycoprotein E, central domain, subdomain 1 / Flaviviral glycoprotein E, central domain, subdomain 2 / Flavivirus envelope glycoprotein E, Stem/Anchor domain / Flavivirus glycoprotein E, immunoglobulin-like domain / Flavivirus envelope glycoprotein E, Stem/Anchor domain superfamily / Flavivirus glycoprotein, immunoglobulin-like domain / Flavivirus glycoprotein central and dimerisation domain / Flavivirus glycoprotein, central and dimerisation domains / Ribosomal RNA methyltransferase, FtsJ domain / FtsJ-like methyltransferase / Flavivirus/Alphavirus glycoprotein, immunoglobulin-like domain superfamily / Flavivirus glycoprotein, central and dimerisation domain superfamily / Flaviviral glycoprotein E, dimerisation domain / DEAD box, Flavivirus / Flavivirus DEAD domain / helicase superfamily c-terminal domain / Immunoglobulin E-set / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / RNA-directed RNA polymerase, catalytic domain / RdRp of positive ssRNA viruses catalytic domain profile. / S-adenosyl-L-methionine-dependent methyltransferase superfamily / Peptidase S1, PA clan / DNA/RNA polymerase superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Biological speciesZika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013
Macaca mulatta (Rhesus monkey)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.803 Å
AuthorsSankhala, R.S. / Joyce, M.G.
Funding support United States, 1items
OrganizationGrant numberCountry
Department of Defense (DOD, United States)W81XWH-07-2-0067 United States
CitationJournal: Cell Rep / Year: 2023
Title: Zika-specific neutralizing antibodies targeting inter-dimer envelope epitopes.
Authors: Sankhala, R.S. / Dussupt, V. / Donofrio, G. / Gromowski, G.D. / De La Barrera, R.A. / Larocca, R.A. / Mendez-Rivera, L. / Lee, A. / Choe, M. / Zaky, W. / Mantus, G. / Jensen, J.L. / Chen, W. ...Authors: Sankhala, R.S. / Dussupt, V. / Donofrio, G. / Gromowski, G.D. / De La Barrera, R.A. / Larocca, R.A. / Mendez-Rivera, L. / Lee, A. / Choe, M. / Zaky, W. / Mantus, G. / Jensen, J.L. / Chen, W.H. / Gohain, N. / Bai, H. / McCracken, M.K. / Mason, R.D. / Leggat, D. / Slike, B.M. / Tran, U. / Jian, N. / Abbink, P. / Peterson, R. / Mendes, E.A. / Freitas de Oliveira Franca, R. / Calvet, G.A. / Bispo de Filippis, A.M. / McDermott, A. / Roederer, M. / Hernandez, M. / Albertus, A. / Davidson, E. / Doranz, B.J. / Rolland, M. / Robb, M.L. / Lynch, R.M. / Barouch, D.H. / Jarman, R.G. / Thomas, S.J. / Modjarrad, K. / Michael, N.L. / Krebs, S.J. / Joyce, M.G.
History
DepositionSep 6, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 30, 2023Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
Z: Envelope protein E
H: rhMZ100-C antibody heavy chain
L: rhMZ100-C antibody light chain
E: Envelope protein E
B: Envelope protein E
C: rhMZ100-C antibody light chain
A: Envelope protein E
D: rhMZ100-C antibody heavy chain


Theoretical massNumber of molelcules
Total (without water)270,3208
Polymers270,3208
Non-polymers00
Water2,774154
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration, Protein complex migrated as a dimer of tetramer.
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)101.840, 126.680, 140.480
Angle α, β, γ (deg.)90.000, 89.970, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain D and (resid 2 through 12 or resid 14...
21(chain H and (resid 2 through 12 or resid 14...

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111(chain D and (resid 2 through 12 or resid 14...D2 - 12
121(chain D and (resid 2 through 12 or resid 14...D14 - 18
131(chain D and (resid 2 through 12 or resid 14...D20 - 75
141(chain D and (resid 2 through 12 or resid 14...D77
151(chain D and (resid 2 through 12 or resid 14...D124 - 136
161(chain D and (resid 2 through 12 or resid 14...D140
171(chain D and (resid 2 through 12 or resid 14...D142 - 218
211(chain H and (resid 2 through 12 or resid 14...H2 - 12
221(chain H and (resid 2 through 12 or resid 14...H14 - 18
231(chain H and (resid 2 through 12 or resid 14...H20 - 75
241(chain H and (resid 2 through 12 or resid 14...H77 - 88
251(chain H and (resid 2 through 12 or resid 14...H0
261(chain H and (resid 2 through 12 or resid 14...H124 - 136
271(chain H and (resid 2 through 12 or resid 14...H138
281(chain H and (resid 2 through 12 or resid 14...H140
291(chain H and (resid 2 through 12 or resid 14...H142 - 218

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Components

#1: Protein
Envelope protein E /


Mass: 44214.059 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Zika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013
Strain: isolate ZIKV/Human/French Polynesia/10087PF/2013
Production host: Insect cell expression vector pTIE1 (others)
References: UniProt: A0A024B7W1
#2: Antibody rhMZ100-C antibody heavy chain


Mass: 23274.891 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Macaca mulatta (Rhesus monkey) / Production host: Homo sapiens (human)
#3: Antibody rhMZ100-C antibody light chain


Mass: 23456.941 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Macaca mulatta (Rhesus monkey) / Production host: Homo sapiens (human)
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 154 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.35 Å3/Da / Density % sol: 63.31 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop / Details: 12% PEG 8000, 0.2 M (NH4)2SO4, 0.1M Tris (pH 8.5)

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Nov 1, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97 Å / Relative weight: 1
Reflection twinOperator: h,-k,-l / Fraction: 0.49
ReflectionResolution: 2.8→14.984 Å / Num. obs: 62002 / % possible obs: 70.8 % / Redundancy: 2.6 % / CC1/2: 0.88 / Net I/σ(I): 11.7
Reflection shellResolution: 2.8→2.9 Å / Num. unique obs: 6273 / CC1/2: 0.499

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Processing

Software
NameVersionClassification
PHENIX1.11.1_2575refinement
PDB_EXTRACT3.27data extraction
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6NIP

6nip


Resolution: 2.803→14.984 Å / Cross valid method: THROUGHOUT / σ(F): 1.24 / Phase error: 32.3 / Stereochemistry target values: TWIN_LSQ_F
RfactorNum. reflection% reflection
Rfree0.2955 2374 4.46 %
Rwork0.2412 49137 -
obs0.2457 53190 61.19 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 186.83 Å2 / Biso mean: 65.451 Å2 / Biso min: 0.63 Å2
Refinement stepCycle: final / Resolution: 2.803→14.984 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms18752 0 0 154 18906
Biso mean---39.71 -
Num. residues----2479
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00319185
X-RAY DIFFRACTIONf_angle_d0.74326064
X-RAY DIFFRACTIONf_chiral_restr0.0462928
X-RAY DIFFRACTIONf_plane_restr0.0063354
X-RAY DIFFRACTIONf_dihedral_angle_d11.74911488
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11D1762X-RAY DIFFRACTION11.797TORSIONAL
12H1762X-RAY DIFFRACTION11.797TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.9005-2.97120.3853940.29961703179731
2.9712-3.04930.2716880.29392722281049
3.0493-3.13640.3331950.28483541373665
3.1364-3.23440.33161010.273939404072
3.2344-3.34590.36642100.26643820403069
3.3459-3.47460.36251020.26143880398270
3.4746-3.62560.30222050.25743674387967
3.6256-3.80690.35651100.25133853396370
3.8069-4.0310.29821000.24693803390369
4.031-4.31920.27242050.23113730393568
4.3192-4.71280.27431100.20613665377566
4.7128-5.30550.25921990.21263646384566
5.3055-6.38640.25791050.24383689379466
6.3864-10.06090.27562020.22823472367462
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.44380.7099-0.12481.2833-0.54560.6978-0.36010.0097-0.31850.0427-0.0578-0.13720.3133-0.0974-0.33370.43060.16470.29480.4265-0.05190.7488-51.9303-14.6226-21.296
20.120.0639-0.04520.08230.07490.24940.28470.0757-0.1354-0.23470.0689-0.0317-0.164-0.12710.17150.37860.02850.05680.46410.17810.4698-37.61342.4583-26.5842
30.19460.09850.01980.4492-0.06740.01340.0511-0.0242-0.28720.01590.0609-0.01730.02180.10530.28280.31920.15780.27420.60770.01721.0881-45.7674-15.5385-16.6202
40.259-0.0002-0.13440.99050.05730.07530.0292-0.088-0.08540.04040.07160.01370.01650.0170.09570.13130.0547-0.04040.43730.20090.5438-42.796724.1882-25.2465
52.1477-1.08480.56970.8366-0.84861.2445-0.2882-0.0651-0.24340.250.09110.31850.2486-0.25140.06220.81750.0518-0.00480.50570.08871.0319-49.88346.1645-21.5287
60.2627-0.1431-0.02190.0889-0.1110.9683-0.0429-0.0233-0.3106-0.11240.0884-0.21070.50050.07160.17340.66270.08790.36860.23520.02610.8656-66.6731-31.4599-10.0544
70.2664-0.094-0.13240.2783-0.08160.3553-0.0520.0772-0.1341-0.0045-0.0986-0.07760.11830.0534-0.32410.1209-0.1410.28760.2484-0.01480.0845-5.887648.6249-28.6865
80.47730.23360.44790.87460.55610.5903-0.1011-0.035-0.00360.01280.1532-0.1043-0.03830.283-0.02960.3065-0.09590.1190.3265-0.02490.2337-4.94950.7848-21.3455
90.1076-0.2182-0.16320.8343-0.66072.71720.1984-0.39430.10720.5480.01250.2093-0.3385-0.1416-0.10940.3987-0.24380.07010.70270.01230.30086.649984.39783.2199
101.60740.06521.12791.2884-0.09592.36780.04050.1571-0.23240.0395-0.01440.05160.33980.3872-0.07240.404-0.2632-0.06590.51350.09490.351212.529673.9613-6.6797
110.88270.57720.2210.97590.5971.79950.0033-0.2263-0.06130.217-0.1696-0.0889-0.06230.26520.00080.3975-0.2698-0.03770.4220.1140.22288.468773.6378-3.2763
120.2173-0.02410.3460.0698-0.0170.5606-0.0602-0.19590.10610.11-0.1060.04-0.26830.0998-0.08070.5027-0.23770.17180.8424-0.23080.436918.927878.0027-5.5232
130.5001-0.10630.03920.7767-0.26460.09570.14860.0158-0.011-0.0110.12820.17710.00040.07550.09730.27920.00180.02360.2896-0.09270.1612-20.089350.8708-9.76
145.4963-0.6260.48630.7215-0.64890.5836-0.0356-0.1838-0.07740.29430.0804-0.06070.05460.14220.030.31980.0516-0.10120.1733-0.06160.3686-20.353838.5919-5.5708
150.54620.0969-0.17910.3518-0.06151.2330.3239-0.00740.1335-0.12810.02810.0183-0.1768-0.0141-0.0160.2165-0.0106-0.00920.26780.00730.1393-19.320351.0791-8.4436
160.46420.5733-0.12780.8729-0.27450.45550.1926-0.3334-0.25370.329-0.3965-0.4235-0.25430.37090.22790.5205-0.22150.13360.365-0.05260.3368-5.239869.99985.2697
170.10030.11030.04540.13290.04210.02290.04120.11810.1646-0.14730.03890.0009-0.0482-0.0205-0.05110.6737-0.21250.00960.39450.18720.4083.623482.4943-7.9477
182.20671.169-0.45920.6526-0.09410.7562-0.0626-0.04780.05270.43250.22130.3587-0.5051-0.075-0.18830.6487-0.02580.1190.51290.1420.2756-9.848373.3671-3.8166
190.64450.0991-0.5850.61910.26650.75360.05330.08980.0471-0.0424-0.0795-0.2095-0.2490.16130.10260.6914-0.09370.18970.6137-0.00040.417-4.282671.4982-5.8942
200.137-0.2598-0.00520.54620.04570.0265-0.01090.06150.25040.08670.02130.1353-0.4146-0.0085-0.10750.8242-0.02760.18990.45450.12690.5257-5.774282.2843-5.9476
210.13120.1151-0.02870.8194-0.05920.22010.1520.15480.04770.2744-0.08250.2591-0.0277-0.20770.04540.43610.14460.41230.50560.1540.7602-66.215638.7046-24.3042
221.26150.3519-0.36841.0246-0.51310.66630.24220.00480.21090.7740.16870.3251-0.414-0.27690.30.27480.11550.04640.24030.17930.329-47.056576.3679-40.8162
230.5565-0.2990.21580.4572-0.35640.5034-0.20920.12360.22520.1282-0.0121-0.0699-0.077-0.0254-0.16180.18090.05240.1720.3767-0.11940.2056.665441.9856-46.0533
240.59810.0676-0.00820.1812-0.15380.4131-0.16920.29530.123-0.0611-0.1111-0.0936-0.00250.1577-0.80860.3098-0.10620.29840.31680.08060.45935.807354.3647-49.3696
250.5212-0.15790.09811.0273-0.45151.2414-0.03610.14930.04060.2108-0.0193-0.2906-0.2820.128-0.33560.4054-0.08650.22690.2819-0.11960.2637-15.69689.7176-60.1008
260.3797-0.2594-0.32510.50480.48640.4918-0.1708-0.07-0.29850.12910.0780.09820.15520.1840.11390.1442-0.03380.11010.4272-0.03870.361632.44991.515-66.3993
271.8022-0.51540.12811.53460.50290.75970.0581-0.17-0.43770.28670.15360.26230.2618-0.01270.20550.14990.14640.06880.29490.06010.436927.16126.914-57.5308
280.33450.163-0.38790.6898-0.04950.5025-0.1560.03020.05060.1240.08910.1955-0.05960.23090.04030.12090.0655-0.00160.41710.03470.290529.71213.2246-62.7228
290.176-0.275-0.42320.47330.72651.113-0.1484-0.0205-0.1790.12190.0863-0.03130.03310.18090.05430.3085-0.0533-0.00670.3797-0.07990.317936.02818.7658-63.6301
300.495-0.5603-0.05070.9350.2310.107-0.16260.0678-0.13380.053-0.0147-0.03630.20280.3766-0.05030.3970.05630.2490.3779-0.10920.476340.1923-3.2614-65.6244
311.2049-0.6770.38780.6309-0.15910.38990.0521-0.2442-0.59980.21320.1281-0.0130.31480.12760.10610.67040.1902-0.05720.6745-0.05850.535649.989-20.8248-63.6174
320.91940.02580.1432.7629-0.41051.9092-0.1735-0.2764-0.3310.42650.08790.11430.52170.08270.22650.42060.28040.14720.6732-0.1520.520847.6957-18.3652-62.0333
330.73770.26-0.34260.26790.21710.8048-0.1124-0.0626-0.53030.090.07370.12720.58320.27090.1180.69760.0549-0.00180.3584-0.06270.70242.1318-24.07-68.397
342.56270.5631-0.32171.60260.4671.3395-0.0606-0.0202-0.2746-0.01090.06260.18720.3686-0.11280.09860.37630.11810.13440.54750.15060.7291-8.5742-8.3812-27.4528
350.2718-0.6150.15061.3967-0.51950.48790.04110.0667-0.4051-0.27630.10260.1120.02510.0651-0.01010.3709-0.0514-0.08230.31760.04550.6134-18.444331.9008-54.4414
360.4311-0.07070.04980.6994-0.14050.5520.08970.0894-0.4168-0.0163-0.0682-0.01690.3445-0.1799-0.56570.3105-0.1548-0.12210.3222-0.12770.408-14.917924.3908-51.5194
370.75920.2902-0.22110.1373-0.18340.630.0003-0.0919-0.20130.24030.1950.21420.0614-0.08150.09590.644-0.0996-0.08160.7818-0.08431.0472-19.3198-3.3034-37.7436
381.2725-0.4137-0.47991.87730.12851.917-0.0569-0.116-0.20730.36560.0294-0.0120.21380.05050.02190.37830.13550.04230.4850.16580.6447-17.189125.0247-45.7502
390.9454-0.39280.17791.4996-0.56570.70260.07290.1471-0.2588-0.0580.18010.13940.3525-0.11840.0590.54920.05350.09110.3433-0.09760.2978-14.436740.5608-54.639
400.14030.0699-0.12420.08270.00720.226-0.17850.0396-0.295-0.0259-0.00360.02940.38020.10360.11421.0450.09780.08550.6850.13991.2373-0.1279-12.8916-30.6476
410.1042-0.0439-0.01850.02360.00280.0066-0.2077-0.19-0.2160.33840.0249-0.04130.17330.24240.10341.39480.07460.17140.86050.12621.27468.0501-18.8159-23.9866
420.92390.1551-0.56870.51150.49881.0937-0.0036-0.0603-0.19840.09960.1089-0.05590.2510.36060.06420.4490.217-0.16640.8095-0.17750.582749.948914.0728-43.9054
430.6837-0.09-0.30580.0252-0.08071.1601-0.0901-0.17-0.16060.19220.05-0.06770.52060.36540.06060.54430.11740.0190.6245-0.23440.327343.05168.3418-40.8967
441.5017-0.7395-0.56142.24770.63991.6581-0.027-0.0054-0.41450.14810.0645-0.02730.54660.43780.11440.35180.10230.00250.8284-0.17440.435847.94484.1491-50.2068
451.14620.0118-0.37820.83-0.21460.3109-0.1959-0.1007-0.20520.09360.02590.17640.16480.28020.15531.15270.1714-0.07581.5472-0.08461.409759.3851-16.5186-64.3541
460.409-0.0757-0.1790.3653-0.15020.1732-0.22190.0282-0.4160.13940.0653-0.02570.39760.40940.07561.32850.33440.02091.6238-0.05811.531866.7577-20.1681-65.2716
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'Z' and (resid 2 through 60 )Z2 - 60
2X-RAY DIFFRACTION2chain 'Z' and (resid 61 through 129 )Z61 - 129
3X-RAY DIFFRACTION3chain 'Z' and (resid 130 through 200 )Z130 - 200
4X-RAY DIFFRACTION4chain 'Z' and (resid 201 through 261 )Z201 - 261
5X-RAY DIFFRACTION5chain 'Z' and (resid 262 through 288 )Z262 - 288
6X-RAY DIFFRACTION6chain 'Z' and (resid 289 through 403 )Z289 - 403
7X-RAY DIFFRACTION7chain 'H' and (resid 2 through 91 )H2 - 91
8X-RAY DIFFRACTION8chain 'H' and (resid 92 through 127 )H92 - 127
9X-RAY DIFFRACTION9chain 'H' and (resid 128 through 142 )H128 - 142
10X-RAY DIFFRACTION10chain 'H' and (resid 143 through 165 )H143 - 165
11X-RAY DIFFRACTION11chain 'H' and (resid 166 through 196 )H166 - 196
12X-RAY DIFFRACTION12chain 'H' and (resid 197 through 219 )H197 - 219
13X-RAY DIFFRACTION13chain 'L' and (resid 1 through 53 )L1 - 53
14X-RAY DIFFRACTION14chain 'L' and (resid 54 through 66 )L54 - 66
15X-RAY DIFFRACTION15chain 'L' and (resid 67 through 113 )L67 - 113
16X-RAY DIFFRACTION16chain 'L' and (resid 114 through 126 )L114 - 126
17X-RAY DIFFRACTION17chain 'L' and (resid 127 through 147 )L127 - 147
18X-RAY DIFFRACTION18chain 'L' and (resid 148 through 158 )L148 - 158
19X-RAY DIFFRACTION19chain 'L' and (resid 159 through 179 )L159 - 179
20X-RAY DIFFRACTION20chain 'L' and (resid 180 through 217 )L180 - 217
21X-RAY DIFFRACTION21chain 'E' and (resid 1 through 277 )E1 - 277
22X-RAY DIFFRACTION22chain 'E' and (resid 278 through 403 )E278 - 403
23X-RAY DIFFRACTION23chain 'B' and (resid 2 through 129 )B2 - 129
24X-RAY DIFFRACTION24chain 'B' and (resid 130 through 288 )B130 - 288
25X-RAY DIFFRACTION25chain 'B' and (resid 289 through 403 )B289 - 403
26X-RAY DIFFRACTION26chain 'C' and (resid 1 through 16 )C1 - 16
27X-RAY DIFFRACTION27chain 'C' and (resid 17 through 39 )C17 - 39
28X-RAY DIFFRACTION28chain 'C' and (resid 40 through 82 )C40 - 82
29X-RAY DIFFRACTION29chain 'C' and (resid 83 through 99 )C83 - 99
30X-RAY DIFFRACTION30chain 'C' and (resid 100 through 126 )C100 - 126
31X-RAY DIFFRACTION31chain 'C' and (resid 127 through 158 )C127 - 158
32X-RAY DIFFRACTION32chain 'C' and (resid 159 through 195 )C159 - 195
33X-RAY DIFFRACTION33chain 'C' and (resid 196 through 217 )C196 - 217
34X-RAY DIFFRACTION34chain 'A' and (resid 1 through 40 )A1 - 40
35X-RAY DIFFRACTION35chain 'A' and (resid 41 through 83 )A41 - 83
36X-RAY DIFFRACTION36chain 'A' and (resid 84 through 163 )A84 - 163
37X-RAY DIFFRACTION37chain 'A' and (resid 164 through 201 )A164 - 201
38X-RAY DIFFRACTION38chain 'A' and (resid 202 through 229 )A202 - 229
39X-RAY DIFFRACTION39chain 'A' and (resid 230 through 268 )A230 - 268
40X-RAY DIFFRACTION40chain 'A' and (resid 269 through 352 )A269 - 352
41X-RAY DIFFRACTION41chain 'A' and (resid 353 through 403 )A353 - 403
42X-RAY DIFFRACTION42chain 'D' and (resid 2 through 33 )D2 - 33
43X-RAY DIFFRACTION43chain 'D' and (resid 34 through 91 )D34 - 91
44X-RAY DIFFRACTION44chain 'D' and (resid 92 through 132 )D92 - 132
45X-RAY DIFFRACTION45chain 'D' and (resid 133 through 185 )D133 - 185
46X-RAY DIFFRACTION46chain 'D' and (resid 186 through 218 )D186 - 218

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