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Yorodumi- PDB-2m3t: Solution-state NMR structure of wild-type human gamma(S)-crystallin -
+Open data
-Basic information
Entry | Database: PDB / ID: 2m3t | ||||||
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Title | Solution-state NMR structure of wild-type human gamma(S)-crystallin | ||||||
Components | Beta-crystallin S | ||||||
Keywords | STRUCTURAL PROTEIN / gamma-S / eye lens / aggregation / crystallin / cataract / CRYGS | ||||||
Function / homology | Function and homology information structural constituent of eye lens / lens development in camera-type eye / visual perception / morphogenesis of an epithelium Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | minimized average structure, model 1 | ||||||
Model type details | minimized average | ||||||
Authors | Brubaker, W.D. / Martin, R.W. | ||||||
Citation | Journal: Structure / Year: 2013 Title: Preferential and Specific Binding of Human alpha B-Crystallin to a Cataract-Related Variant of gamma S-Crystallin. Authors: Kingsley, C.N. / Brubaker, W.D. / Markovic, S. / Diehl, A. / Brindley, A.J. / Oschkinat, H. / Martin, R.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2m3t.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2m3t.ent.gz | 978.4 KB | Display | PDB format |
PDBx/mmJSON format | 2m3t.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m3/2m3t ftp://data.pdbj.org/pub/pdb/validation_reports/m3/2m3t | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 20959.633 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CRYGS, GRYG8 / Variant: Wild-Type / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta (DE3) / References: UniProt: P22914 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions |
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-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 7444 / NOE intraresidue total count: 1547 / NOE long range total count: 3052 / NOE medium range total count: 1286 / NOE sequential total count: 1559 | ||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 21 |