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- EMDB-22679: Structure of outer-arm dynein bound to microtubule doublet in mic... -

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Basic information

Entry
Database: EMDB / ID: EMD-22679
TitleStructure of outer-arm dynein bound to microtubule doublet in microtubule binding state 2 (MTBS-2)
Map data
Sample
  • Complex: Outer-arm dynein bound to microtubule doublet in microtubule binding state 2 (MTBS-2)
    • Protein or peptide: x 18 types
  • Ligand: x 3 types
Function / homology
Function and homology information


dynein complex / minus-end-directed microtubule motor activity / dynein light intermediate chain binding / motile cilium / dynein intermediate chain binding / microtubule-based movement / microtubule-based process / microtubule / calcium ion binding / ATP hydrolysis activity ...dynein complex / minus-end-directed microtubule motor activity / dynein light intermediate chain binding / motile cilium / dynein intermediate chain binding / microtubule-based movement / microtubule-based process / microtubule / calcium ion binding / ATP hydrolysis activity / ATP binding / cytoplasm
Similarity search - Function
Dynein light chain Tctex-1 like / Tctex-1-like superfamily / Tctex-1 family / Dynein light chain type 1 / Dynein light chain, type 1/2 / Dynein light chain superfamily / Dynein light chain type 1 / Dynein heavy chain, AAA 5 extension domain / Dynein heavy chain AAA lid domain / Roadblock/LAMTOR2 domain ...Dynein light chain Tctex-1 like / Tctex-1-like superfamily / Tctex-1 family / Dynein light chain type 1 / Dynein light chain, type 1/2 / Dynein light chain superfamily / Dynein light chain type 1 / Dynein heavy chain, AAA 5 extension domain / Dynein heavy chain AAA lid domain / Roadblock/LAMTOR2 domain / Roadblock/LC7 domain / Roadblock/LC7 domain / Dynein heavy chain 3, AAA+ lid domain / AAA+ lid domain / Dynein heavy chain, C-terminal domain / Dynein heavy chain, C-terminal domain, barrel region / Dynein heavy chain C-terminal domain / Leucine rich repeat 4 / Leucine Rich repeats (2 copies) / P-loop containing dynein motor region / Dynein heavy chain, tail / Dynein heavy chain, N-terminal region 1 / Dynein heavy chain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, linker / Dynein heavy chain, AAA module D4 / Dynein heavy chain, coiled coil stalk / Dynein heavy chain, hydrolytic ATP-binding dynein motor region / Dynein heavy chain, ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Dynein heavy chain AAA lid domain superfamily / Dynein heavy chain, domain 2, N-terminal / Dynein heavy chain, linker, subdomain 3 / Dynein heavy chain, AAA1 domain, small subdomain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, N-terminal region 2 / Hydrolytic ATP binding site of dynein motor region / Microtubule-binding stalk of dynein motor / P-loop containing dynein motor region D4 / ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / EF hand / Leucine-rich repeat, SDS22-like subfamily / Thioredoxin / Leucine-rich repeat profile. / Thioredoxin domain / Leucine-rich repeat / EF-hand domain pair / Leucine-rich repeat domain superfamily / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Thioredoxin-like superfamily / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Thioredoxin / Dynein light chain tctex-type 1 protein / Dynein intermediate chain 2 / Outer arm dynein beta heavy chain / Dynein light chain roadblock-type 2 protein / Dynein light chain / Dynein light chain / Dynein light chain / Dynein light chain / Dynein light chain 4A ...Thioredoxin / Dynein light chain tctex-type 1 protein / Dynein intermediate chain 2 / Outer arm dynein beta heavy chain / Dynein light chain roadblock-type 2 protein / Dynein light chain / Dynein light chain / Dynein light chain / Dynein light chain / Dynein light chain 4A / Dynein light chain 2A / Dynein light chain 1 / Dynein heavy chain, outer arm protein / Dynein light chain roadblock-type 2 protein / Dynein light chain / Flagellar outer dynein arm intermediate protein, putative / Dynein light chain
Similarity search - Component
Biological speciesTetrahymena thermophila (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.5 Å
AuthorsRao Q / Zhang K
CitationJournal: Nat Struct Mol Biol / Year: 2021
Title: Structures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism.
Authors: Qinhui Rao / Long Han / Yue Wang / Pengxin Chai / Yin-Wei Kuo / Renbin Yang / Fangheng Hu / Yuchen Yang / Jonathon Howard / Kai Zhang /
Abstract: Thousands of outer-arm dyneins (OADs) are arrayed in the axoneme to drive a rhythmic ciliary beat. Coordination among multiple OADs is essential for generating mechanical forces to bend microtubule ...Thousands of outer-arm dyneins (OADs) are arrayed in the axoneme to drive a rhythmic ciliary beat. Coordination among multiple OADs is essential for generating mechanical forces to bend microtubule doublets (MTDs). Using electron microscopy, we determined high-resolution structures of Tetrahymena thermophila OAD arrays bound to MTDs in two different states. OAD preferentially binds to MTD protofilaments with a pattern resembling the native tracks for its distinct microtubule-binding domains. Upon MTD binding, free OADs are induced to adopt a stable parallel conformation, primed for array formation. Extensive tail-to-head (TTH) interactions between OADs are observed, which need to be broken for ATP turnover by the dynein motor. We propose that OADs in an array sequentially hydrolyze ATP to slide the MTDs. ATP hydrolysis in turn relaxes the TTH interfaces to effect free nucleotide cycles of downstream OADs. These findings lead to a model explaining how conformational changes in the axoneme produce coordinated action of dyneins.
History
DepositionSep 16, 2020-
Header (metadata) releaseSep 29, 2021-
Map releaseSep 29, 2021-
UpdateOct 20, 2021-
Current statusOct 20, 2021Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.7
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 0.7
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7k5b
  • Surface level: 0.7
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-7k5b
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_22679.png

Downloads & links

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Map

FileDownload / File: emd_22679.map.gz / Format: CCP4 / Size: 193.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.333 Å
Density
Contour LevelBy AUTHOR: 0.7 / Movie #1: 0.7
Minimum - Maximum0.0 - 8.594368
Average (Standard dev.)0.028575433 (±0.18947779)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions370396347
Spacing396370347
CellA: 527.86786 Å / B: 493.20984 Å / C: 462.55084 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.3331.3331.333
M x/y/z396370347
origin x/y/z0.0000.0000.000
length x/y/z527.868493.210462.551
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ256256256
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS396370347
D min/max/mean0.0008.5940.029

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Supplemental data

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Sample components

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Entire : Outer-arm dynein bound to microtubule doublet in microtubule bind...

EntireName: Outer-arm dynein bound to microtubule doublet in microtubule binding state 2 (MTBS-2)
Components
  • Complex: Outer-arm dynein bound to microtubule doublet in microtubule binding state 2 (MTBS-2)
    • Protein or peptide: Dynein heavy chain, outer arm protein
    • Protein or peptide: Outer arm dynein beta heavy chain
    • Protein or peptide: gamma heavy chain
    • Protein or peptide: Dynein intermediate chain 2
    • Protein or peptide: Flagellar outer dynein arm intermediate protein, putative
    • Protein or peptide: Dynein light chain roadblock-type 2 protein
    • Protein or peptide: Dynein light chain roadblock-type 2 protein
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain tctex-type 1 protein
    • Protein or peptide: Dynein light chain 2A
    • Protein or peptide: Thioredoxin
    • Protein or peptide: Dynein light chain 1
    • Protein or peptide: Dynein light chain 4A
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION

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Supramolecule #1: Outer-arm dynein bound to microtubule doublet in microtubule bind...

SupramoleculeName: Outer-arm dynein bound to microtubule doublet in microtubule binding state 2 (MTBS-2)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#18
Source (natural)Organism: Tetrahymena thermophila (eukaryote)

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Macromolecule #1: Dynein heavy chain, outer arm protein

MacromoleculeName: Dynein heavy chain, outer arm protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 533.780188 KDa
SequenceString: KYSKRIAWMK TTICDSLQLK DMIVEESFQY EKNKNLLEQF LSGEGLNKIF AYYQVQEQAQ NDDIKDTGAQ DPVLFFTTGD LEKIQDKAV WFLRITNPAD DKKKASQQDG NDNDIIFGEI TPNTVPMLNA LMESVYSRQI DHIITEKIQF WGVAEEEQVL E FQQHSNKF ...String:
KYSKRIAWMK TTICDSLQLK DMIVEESFQY EKNKNLLEQF LSGEGLNKIF AYYQVQEQAQ NDDIKDTGAQ DPVLFFTTGD LEKIQDKAV WFLRITNPAD DKKKASQQDG NDNDIIFGEI TPNTVPMLNA LMESVYSRQI DHIITEKIQF WGVAEEEQVL E FQQHSNKF SSEVREAINL MSPGTEHFKL DYEAISGLSE SEKMQHYEMK FNEWINLISS QLNDDSEVRK DEKDAGPATE LI YWRSRMQ KITNWSEQLK SKDFQIVKAS LQRHKNHDNQ RPRGDESLSK LMMEYNRLDL LLTDKLNEAK DNVKYLTTLE KFI EPLYNG TPQQIIDTLP ALMNAIKMIH TIARFYNTTD KMTGLFIKIT NQMIKNCKDR ILNKKDNGDN PSLYKMIWEQ DPAE LIEVL GSCIKLYCEY KKCYNDTKEK VADMPKGKTF DFSDAQIFGK FDTFVRRLQK LIEIFSNIQQ FNALAKHNLE GMDVL TNKF KKIIDDFKKK GHNLLDTANN KFDRDWVEFN VEISHLDGEL QNFIDNNFNR FRNIEYSLKL LHKFQSTIKR DSLKHN LTS RYNAILHNYA TELDTIQRVF QDQKSNPPLV RNMPPEAGKI IWARHLFQKI TGPINIFPEN VINSTEIRRY YGSYNTL GK QLTIYEMWFY QDWVNKIEQS KAALQATLIV RHDENKKLYV NFDLEIMQLI REAKCLDRQG IEIPESARII LLQEDKFK T YYNELLYALK EYERINSKIK PICKNLLLPH IEDLDLKLRP GMVTLTWTSM NIESYLYYVH QGLKKLEQLI INVNDIIEN RIENNLKTVS KVVLVHLPQD TKPLSLDSFV QLQEEYINSK TDFLTSKNVE VERAVDDLLQ TIMLYPLDPH VDPVLPEETK RIKRYYFWY FYQALLNSTQ NSLNAMKYRV CGKKIPGANT LQNLKPFFQV EVQLNGDKVT LNPSLQEIQK SINRAATAVL R CSKHLYNW DQQNKDSTDK ATFYDMIACD KEIVKVILLL TGSIQGTKNK VNEFLSGFTK FEWLCKESIQ ESIKKFSKNG PT LQNYEDQ LKKFSQIEEE IEKIVPTYKI GAMELMTHNI CTSLSTWAKE WKLQYSQDLH KRARQLLDSL TEQTKMLSTK LSK PVKDID SLGYVMETLE QIRKEQAEID MKFNPVQEMY SLLDNYLPGG ITDKDEMDAR SLLRRNWDIL IQQAEIKGKE YQHK QAIYL KELKQSIKDF TNQVSIFRRD YEKNGPMVEG ISPAEAMERL RRFEDEYDVK YQMYKINARG ENLFGLQNQK YPELE KTDA EIKNLNKLYN LYDSVIKNIQ QFKEKSWQDV SKDDLAKMEE DAGKYGEQCS RLPKDLKEWQ AYRDLKNYID SLREQL PLI ISLKKPSIMP RHWEKIKEIT NTKLNYENPD QFYIEEIMGA KLLDFREDIE DITESADKQL KIRTGLDEIN LYWNDMQ FQ FGIWGKRDVP CMLNGLIVGT ILERLEEDQL QLSTFNSQRH VTPFKAEVEN LIRTFSDVND TLDMWVKVQK LWTSLEPV F TGGDIARQMP LQAKQFQGID KNWMKIMEKA VETKKVIPCC QNDMLKDFLP DLNRKLEDCQ KMLEAYLEGK RKKFPRFYF VSNPTLLKIL SQGSEPTSIQ EDFEKLFDAI TKVTFESAKD KKNPALKQIT QIQQVIGRNE ENISLTGYYV KCEGNIEDWL KKLEQNMQQ TLKDIASAAA QQVFQVGLKE FVSSQASQIA LLGLQILWTS KVNEGLERLS RNERNAMDIK RNEIKEHMNI L SSMCLEDL NGAVERTKVE TLVTIQVHQK DISMDLKCKD VNDFEWQKQT RIAWKTDIDE CIISITDWDS PYSYEFLGAK ER LCITPLT DRCYITLAQA MSMYYGGAPA GPAGTGKTET VKDLGRTLGV FVVVTNCSDQ HRYRDMAKIF KGLVQSGLWG CFD EFNRID LEVLSVVAMQ VESITTARKQ HMKKFMFPEE EIEIELIPTV SYFITMNPGY AGRQELPENL KVLFRGVSMM VPDR EIIIK VKLASVGYLQ IDLLAKKFNV LYRLCEEQLS KQRHYDFGLR NILSVLRTAG NTKRQEIKSD EEMLLMRSLR DMNLS KLVA DDIPLFNGLL ADIFPKLKEV PKKLYPDVEK KIPEEINAES YLINTPSFQL KIIQLYETCL VRHGFMLVGP TGSGKS TIM KILTEVLTKL GSPHKIVIMN PKAITAEQMY GVKSEISDDW IPGVFSTIWA KSNNRALKHT TWITCDGPVD AIWIENL NT VLDDNKILTL ANGERIAMTE NCKVVFEVEN LNNASPATVS RCGQVYVSPT DLGYEAVIEG WIRNRKASGR AEESDKLG N ILRKYLINMR FIELQSKECK EPMMDTSPVI SVINILNLLT GCLQYFVQTQ RTLSEQEYEK FIVYSMAWAI GGIYEAQDR VRFHELLLAK NAPIPQKGKE NETVFDYYVS QDYLDWKICS PEEWVPPQSL QFSQLLLPTL DSFRAEMLLN FILTQPKSHT CSNSALLIG GSGTAKTSSV LLYCNKFDPQ KMLFKRTNFS SATSPFMFQS TIEAECDFKV GKEFAPPGNK MMTIFIDDMS M PFVNKWGD QITLELVRQL IETGGFYMLD KTQRGNQRKM KNLQYIGAMN HPGGGRNDIP NRLKRQFFIF NMILPLSIEG IY GPIIKHM FKQKYFSDST YKVIESLTSA TIALWNKVKS TMLPTPAKFH YVFNMRELSR IFKGILTCKK DTINDAPKSM KIK PELFLV GLWRHEAERV LADKLVNNKD KDTVMGYIQE VSLESFSQIE NEILEKYSSE KTFLFCDFLR PDVINEDGII EEEA PKIYE AIDSLTELRK RCNFLLSFYN DRNPSKKMPL VLFDDALKHL LRISRIIRQP RSSGLLVGVG GSGKQSLTRL AGFIG KNLI QQIIVTKTYS DKDLKEDIKK GFDDAGHLGK QVTFLMTDSE VKKEEFLEYI NMVLSTGEIP NLLAKDEREV WLGDIS QAY CKEKNLGNID PPQSELWTYF VDRVRDNFHI MLCFSPVGQK FRERARKFPA LFNECTIDWF LPWPEEALVS VAETFIK NF DKLDTKEETK QELMKHMGNV HLMVNEICDE YYQKMRRQVY VTPKSFLSYL NSYKTLYIEK YDELDQQEES FKIGLNKI Q EATITINQME ISLKEEEIQL NEATEKTNQL LANLDKESKK ANQKGEEVAA TNKQCEIQAE QISKEKEEAE RELEAALPA LRRAQEAVDS IESKDIVELK ANKKPLDIIK YIMNAVLVFF KARLIPIQIE ERVFNKKEGK AVLFLKESYD ESGIQTLGDM NFMKKLKEF EKDSINEETI ELLEPYLNQS EDWFNDTFAT KASKAAAGIL KWAFAIYEYH QKSKIVKPKR IQVAIAEGRQ A IALKELEK AREDLAQIQA YIKNLKDVYT KQMEEKNELE MKAAKTKKKI NTARTLITSL SGEKDRWGKG AQDISDQKRK LV GNVSLST AFISYCGPFN AEYRNKLAQQ RFVVDMKKRG VPVTPGLELT SFLIDDATIG EWNLQGLPKD DLSIQNGIMV TNS ARYPLF IDPQGQGQNW IRNKLSASII PERCITTLSH PKFKDMFLKY CMESGLTLIV ENIENEVDPM MDPVLERQII VKGK TQFVN VAGTEMELSK EFKLFMTCRL ANPSFSPELS AKTTIIDFTV TQSGLEQQLL GKVISKEQKA LEDSLNQLLA DVNQN QKDL QRLDKNLLER LINSQGNLLD DTELMDVLNN TKTQAKEVAA KLIDAEIKTK EINEKREQYR PVAIRGSAIY FTMIEV SLV NWMYNSSLEQ FLKLFIESID LSEKAQLPSN RVKNIISFLT FHVYRYVNRG LFEKDKITFI LMMAFKILTT AGTISSG DV SLFLKSGDAL DIKSERQKQI SYLEDNQWLN ILALSKHTFS GQTLPFFKEL PDLISRSENQ WRNWIDKNDP ENFPIPDF A ESINQEKEIG SFISLCLVRS LRNDRTLIAT QNFISNVLGK EFTDPISYPI EGIWQESSNM DPVLFLLSAG ADPTSSIDE LAKKKKKFPC EKVSMGEGQE RVARQVIMKG FVEGGWVILQ NCHLGLKFME EIETLVSPIN QIHEDFRLWI TCEQHPKFPL GLLQKTLKV TNEPPKGLKA GLYKTFTTII TQEFIDKVDH SNWRSLIFTI CFLHSIVIER KKFGPLGWCV PYEYNYSDLE A SLLYIEKY LTNLMSTPQP NSHNLPISMN VVRYMICEVQ YGGRITDDLD RELFITYGET YLKDGIFGND YFFYDIMVDG SG QKFKYRI PQNPSAELIK YQEYIAKVPT VDNPEVFGLH SNADLTFRLK ESKEMINTVM ETRPKDSSVG GGKTREEIVQ DKA KDMLKN LPPDYNDVEV RELVSKLGGP NPKTSTERGM TVPLNIFLYQ EVTRMQRVIG LVRKTLQDTI LAIDGQIIMT PEIL EAINA IYDAKVPNSW LYDPSGAEIS WLLPNLGSWS TSLSDRNKQL NDWLRSGQRP ILFWLTGFFN PQGFLTGMKQ EVTRN HKKG DGKGGEAWSL DDVVYSTTVK EREKEKDIEQ PPAEGVYIKG LYLEGCKWSK NGLDDSDPKK IFADLPILHV SAINKK KTN EQDRMSNTYL CPVYKYPKRT DKYLIFRVGL PCEGSNNPSH WKLRGVALLC ST

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Macromolecule #2: Outer arm dynein beta heavy chain

MacromoleculeName: Outer arm dynein beta heavy chain / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 529.207188 KDa
SequenceString: MGDHSQKDSP EDFIINRLSQ ALGIQKEKIK KSLETAQDDK GEVTNKDEFQ GFIQQDNSTN ILWVSGQSEK CTFYYGQLPP IDKFKKKGI AVIKLGLHKL TNENVAKDVV VVEITNNLLE HLNSVFNEIM SPVMQNPLNQ QGWTDLVAKD LMEKFNNYVA Q VYVLLGQI ...String:
MGDHSQKDSP EDFIINRLSQ ALGIQKEKIK KSLETAQDDK GEVTNKDEFQ GFIQQDNSTN ILWVSGQSEK CTFYYGQLPP IDKFKKKGI AVIKLGLHKL TNENVAKDVV VVEITNNLLE HLNSVFNEIM SPVMQNPLNQ QGWTDLVAKD LMEKFNNYVA Q VYVLLGQI KGKTMLPLPS HKLTSSDTTP DKDKAHVFEG SIITWTKQIK NVLKLEPEQL LKYGNDPGPL AEIEFWQNKR DN LNLIDSQ LKSVEVQNIL HFLDNNKSTY TTPFTKLQAE VKKARLEANE NYRYLFTLKD LFSKLQESQP SDFPTLYELF IPI MHTILL IYNKSKTYNQ PPRLVVLIRE ICNAIISNAQ AFVDKDTIFS LIDSKETTEA CDKLQVTLDV CSKFKDAYFE YKAK AGGNW KLTSNALFVR LDSFLERCQD ILHLTNTIVQ FNKLEKIELG GTKGKTLTES IAQIFKEFEE AVQAFTSVSY DIMNI AEKK FDDDFYEFRS KIKELERRLA SVITQGFDDY DTIYGRFKLL DNFEGLLTRP IIADELEKKH IVLLEMYKQD LKQVQS IFL EGKQFVDSMH ENAPLFLNMP PIAGALTWCK SLRDRIQEPI EKLAQLGQGI TEREEYKDVQ KLYTSITKSI KDYEDQK IL SWEKEVEDSS QDKLKQTLLC KDENDLIKVN FDPSLVRLLK EVKYFLLLRL EVPTTAKDIY TKAETYRTQI VALDMIVD N YNHIKTCLLP VEEPLVKKKI QDMEEEVKPG IEEIRWKSTN IDQFISKSKS IVDQLFETVN KMKDSLQKIH KSLANFNVK IIERKNRPMS PDDYDQFLKA IFSNKLTIVK DNGNQIQKLV KEVLDAVKAD KKQNSWKNYN DYVNVIVIEG ISTAIQTALL HLNEQINPV FIKRNDISPL FDIRLELGQS GIQFDPEIGE SSNQLTVRNT IRNWINDFFN IAGTIQRLDT TMPGDFLQEI R SFFEIKQC LAMITQNLEW IENECNQFRA RFDTYSYLWT EDEQISFNRF LDENEPKDED GKGGDDDEGE NTEKQNPLLK GC RAKIPNL DLFDEKITHL KAIQQEISRI KTPEDISWLR INLQPMKTAL DARVTRWIRV YTDFLVNQFR TTQKNLLDFI EKT KDGIKK NPADHENLHD KKLLMSVMKV ISDVKDVEPR REGIITRMKE MVTKLKKHNV PITEKGTDDP LQQIDNANSN FIEI YGRVF KVKADIIPLQ AEETQNIKRD LDIFMKEVES FRKEFMQKLP FDYTESMGYE NINNAYDTIM VYYHKLTAIE GRALE YNNL EKAFELQKSN YKQLKDCMND LKNLKTMWDA IALIHFQYND WKTKPWRQIK ADILLDTNKT LGTQIKNLPK EIRNFK GYN VIVEKVKNMG TVLPLVSALH SEFMEDRHWS QLKQITGTVF DHNSLSFYFE DILALNLYKY ENTVNEIVDV AQKEAKI EK KLKNIEQWWS KQVFEFTEYK ETKTFASLDN MMEVLDQHSL DLMGMKSQGK YVEFFYDRVE DWREKLGRVD VVVNEWLK V QKNWKILYNI FLLSEDIRMQ LPEDTKVFEG VDKEFKDMMS EVSANPSVVE ACTIERRDVL VGWSQAIKKC EKALNDYLE QKKKSFPRFY FLSNQSLLTI LSNGQNAPKV CEYLGDCFDG LKTLTFEPPA NPAETSKVGI GMISKDDEKV PFSSKFICEG AVEHWLLNL EFRMRETLQE ILEGAKNTAD LWDSGDKPRE EWVEGYNAQI ALLTTTIVWT EDVGRAFEDL AGGSETAMKE C QKLIEVRL ENLIKKVRGD LHILERWKII NIITIDVHSR DVVEKFVIQK VSEAESFAWL SQLKFYWENK PDSDMHLRQT LR FPWEKDK NKNKCIIRIV DWFRFYSYEY IGNAIRLVIT PLTDRCYITL TQALNLTMGG APAGPAGTGK TETTKDLGRA IGI PVMVFN CSDQMNKDSM AQIFMGLSQS GAWGCFDEFN RISIEVLSVV STQVKCVLDA LKEKKTKFSF VEEGEIQLQD TVGF FITMN PGYAGRTELP ENLKALFRSC AMVVPDLALI CENMLMSEGF TMARVLSRKF VSLYMLSREL LSKQKHYDWG LRAVK SVLR QAGKLKRGDP DMPEDPLLMR ALRDFNMPKI VTDDKVIFRR LIGDLFPKLD PPTKQNPELK KIVQDTTKKD MGLVAE ELF VTKVVQLAEI LEVRHCCFVI GPPGSGKTCV WKTLIKSYIN SGEDAEYDTL NPKAVTSDEL FGAYTKTKEW KNGVIAV IM KNQVKNEEKY KATHMHKWSV LDGDIDPEWI ESLNTVMDDN KVLTLVSNDR IFLTPQMRLI FEISNLRNAT PATVSRAG V LFINETDIGW MPYMNSWLER SQINILKQQK EMANMPEYPV IDDVAKSVFY RCFQSYFEQN IDVHDKNRVR HICPMVDIA MIQTICTILD ALLIQHLPKL KQMKEEDEKQ ALEAFFIFAG LWAIGGPVGG GQDDSKDMKE FNTVWKGAAK VKFPEQGLCY DYYYDINEN KWNTWKVEDY LPNDQPLFSK IYVATIHTTR LRYMIDIHLQ RRKPILFIGS AGTGKTAVVR DYLNSTRPEQ V SHKTINFS SFTDSLALQK NIESMVEKKN GRNYGSATNK VLICFIDDFN MPYVDKYGTQ SPIQLLRLIL DYGSIFNREQ LE ERKFLQD LLFFGCLNQK SGSFTVDLRL QRNFSVFSMY TPSSDVIKTI FGSILNAHLS TIDDKAQKMA FKLVEATYFT FDK ILKNTT AFAPSAKRFH YQFNFRELAR VCEGICRTTP GQYSGGDQGK LVRLWAHEMK RTFEDRFIAN EHVEFFRRYL TEAI SKCIG EFPETENPIA EPLIFTGFVA AHQGLDQQYT QCTIPVLKRV LDDKLEEYNE VKAQMNLVLF QQAMEHVSRI CRILD MPGN NALLVGVGGS GKQSLCRLST FINGFEIDQL VVTASFTIND LRNNLQEIYK KIAKPNSIAR VFMITDSQIK EEQFLI PIN DMLNSGWIFD LFPKEDMDSL VSGVRNEAKG EGVDVNNLTA LTSYFLDKIR KNLKVVLCFS PVGDTMRIRS RKFPGII NN TSIDWFHPWP HEALIDVAFR FLEEIEFPTE EIRQSISLNM AKVHSSIDTA NEKFLKLERR YNYTTPKSFL ELIDFYKK L LTEKRETIQR QIQRYEMGLN ILAETQNKVQ GLQEELKVKM VEVNKQREET DILIEKVGKE SALAEEEQTI ANAEEEKTN VAAAEAEKIS KEATEALAEA LPALRSAEAA VDCLKKPHVT EMKNLGSPPA GVIVTARVVL ILFNQGITLN DPDEKVWKKA VTFMNNPQA FIDKVKSFDG ENIEPNIIEQ SNKIIQDPSK KFNEKDMAGQ SYAASKLCAW AVNIVTFNKI FKQVKPLQDA Q KQANEILE EKKKELAIVK QRVAELNARV NSLKRQLEEA EARKMIVEQD AARCQSRLSA AENLVNGLAG ENKRWTQNVK FL KENIKSM IGDSLLASAF VSYIGAFSAK LRLELWKNTW LPDIIEKGIP ITEGIEPLKI LTTEAIKSKW KNEGLPADPM SLE NAAIIT ACARWPLIID PQLQGSTWIR GKQGENLTTI SLSQPKWLGA LTSSISSGRA VLIEGIQQEI DATLDPLLQR AVKK NGNQL QLEIGGDPID YDPNFKLFLM TKLINPHFRP EIAAQCTIIN FIVTESGLEE QFIAMVVNIE KNELEMAKQD LVKKQ NEYA VTLDKLESDL LQSLSEADPA TILDNTELIQ NLDKTKKTTI EITEQQQKAK VTEAEINIQR EHYRVVAAEG SMLYFL VIS LSVMDHMYQY SLESFITFFF KAINRTTVRD ENRIPTLILN IRQTIYQWIS RGLFEKHKLI FLTLIVFRLM QKKIIDV AY EVAEMDFLIK CPARPGVENT LDWLPNISWD QIQGLINLEE FRNFAHQLEK EAPNRFKDWY NELQPEDQKL PLDWKRLD S MPFKKLLVLR CLRPDRMTIS LNNFIRAVLP QGDAFVEMDQ KLAFSEILES VINEDAESTI PIFFILSPGS DPVKEVEKI AKKKRIEPGK NFFNIALGQG QDEIARRRIE EGNKEGHWVM LQNIHLMPTW LIELEKILDS YSGEAGGGNS EFRLFLSAEP STGIPIGIL DRSIKLTNEP PAGLKANMKR AWTYFSKEEI EDKDPKIKSI LFALCFFHST LIERRRFGPK GWNMSYPFNM G DLRDSYLV MNRYMEQNQG GKVPFNDLIY IFGEIMYGGH IVDDWDRRLC NSYLFNTMHE QLFDELELFP YIEGKGLSFK VP GQNPYEK YIEHIETSLK QETPLAYGLH PNAEIGFRTD QCKTLFNTLL ELMPKEQSRD EKSSDIKSSN EMASDLIKQL LED SELKNK IFNMEEIKNK IDAENKGPYQ NVFLQEIEYM NALLSEIVKD LEEIGQGLSG LLTVSENMEM IIESIALSRV PASW QKLAY PSKRGLQSWL ANLFQRIEQL NIFRDDPYSI PRVVMISRFF NPQSFLTAIM QVISRAKAYE LNKLYIQTEI TKRSI EEIE GAAKEGAYVY GFILEGARWD YQLGQLEESK PKEMFSVLPV TYCKAIPLPP EGKEDKSLYQ CPVYKTEDRG NTYVFT AQL KTRFPPRKWI LAGVAIIMDV EGVSD

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Macromolecule #3: gamma heavy chain

MacromoleculeName: gamma heavy chain / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 450.623219 KDa
SequenceString: PLVWTQLKQT GTTQPTARSG HTIITVGKTH IMFGGLDNDK NNYKDGKIAP NNQVFTLKLT QNNCEWRQIA CQGDVPLPRC YHASCAISA DKMLVFGGSY TSNLRFNDTY ILKTTSYQWS KPANQISGGE PKNAESKIGA PQPRYGHSAT FFEGKVYIFG G HGGINYQR ...String:
PLVWTQLKQT GTTQPTARSG HTIITVGKTH IMFGGLDNDK NNYKDGKIAP NNQVFTLKLT QNNCEWRQIA CQGDVPLPRC YHASCAISA DKMLVFGGSY TSNLRFNDTY ILKTTSYQWS KPANQISGGE PKNAESKIGA PQPRYGHSAT FFEGKVYIFG G HGGINYQR LAFNDLYVLE TENFEWTRLE PKGNPPDPRG GHSAAMMANK PQLMIFGGWS FTSQYSNIMI YDIEKDEWVD PE IAHEIPK WNLSGIMAPS IPSWKYFIFG GSVGSFEEGG NRTNSRFVDD SFVLDIDTLS WSSINLEADE TSKAVCKPRP RES ASIFYD SGESRAIVFG GWANNWLNDL WALNVSTITG PPIFSIKPAL GPLTGKTKVL IEGDGFKDTQ NISVKFSGGK LEKE VNGTF VNEKEISCET PTFDYPRSVE VTVCMNKGDY TITKSAFTYN TKADKTIAYG PGLLTENLIG VQTTIVIQAR NGSDE FVVT IRNPAKIKKE EEVKEGDKAN TKNTIKEDEE EEGEDEEENK KKKEAEKNDL TGSAVMNYIS KQLKDIQEFI ENTKEN IEI RNKNISELIN VMINLEKVRV KNDDDVLTLD TVEEMLNFLK KKKDSDIKKC KKLQEEWKNL AKMAQAVKKD IQNPVKT ES DKTKENIKKF EEITLKEYAN SLKKESFFIY KTGVSESFKR IGEVKQKVDE FEVQLNQYED FARIGSKKLM EQIRTDVS S VEKLWVRIEI SEKTMDEYKK MKWGSINSMD MEDEIKKLRK ALTDLRGIDK RSNAFIGITE ELKKWATFLP LLGELKDPS MNSEDGRHWK KLKDLVKKEF DVSQELMLEI IWDLKLFDYK DGIEEITDQA KQELKMEKAL KKIIDFWRDI EFELVQHKNT DIHTLKMSE ENFETLEDHQ LQINNMLLSK YVAYFEKEVE KWKYDLGSVY DVVQLLLEVQ KTWSFLENLF EVKRELPNES A QFVGIDKD MKEIMQKGCD IKNCLKFCTI EGMLKRLENI QAQLKVCEKA LNEFLDSKRR AFPRFYFVSV NDLLDILSNG NS PAKINRH MSKIFQAIDN LQLKEDSSGG RPTLKMISCV GTEEVDFSSP RLLQGKVESY LKDVIDTMIG TLKSVANSSF KNF QSMTRK EWLKSDPSQI TLLVNNIIWS KAVEDCFLKL QSGDINAMKL FLDESIKQLT ELIGMVQGDL SKPLRQKIMC LITI DTHSR DVVHRLINEH VRKAEEFQWQ SQLKFYWVDN DAKIKIADAR FVYNYEYLGN GPRLVITPLT DRIYVTATQA LHLKM GCAP AGPAGTGKTE TTKDLANALA KACYVFNCSS EMNYESMGNI YKGLASSGCW GCFDEFNRLL PEVLSVCSVQ FKAVTD AIK QNVERFIIEG DEISLDPTCG VFITMNPGYL GRAELPEGLK ALFRPITVVV PDLELICENM LMAEGFIEAK ILAKKFV TL YMLCRDLLSK QLHYDWGLRA IKSVLVVAGG FKRSEPEIAE QALLMRALRD FNIPKIAFQD LYVFHGLLGD LFPGINIK P KKDLDFEKII TDVCIENKLD PDPEFVLKVV QLSELLAIRH CVFVMGPPGA GKSTTWKILA KAQDKTNKKT TLIDIDPKV VSTKDFYGYN LPSKEWKDGL FSKMLRSLAE QPDTNPKWIC LDGDLDANWI ESMNSVMDDN KILTLANNER IPLKPHMRAL FEIRDLRFA TPATVSRAGI LYISDEVGYQ WRSYVKSWIK QEFSQDQEMS KNLDTLFGKY VPDTLDHIKK HCRFLVPVSP I SQVISICK SLQTLLKGDV KNLEYLFVYA LIWAIGGALA EKDSIDYRKD FSTWWKGAWK TAVKFPSKGT IFDYYVDQSG DS SKFVEWS KRLENKEFDP QVETMGNITV NTIETLATTE FIKSYLMVKH PSLLIGNSGC GKTQLAKGIL KEIVQAKPEN YAY QLINFN YYTDSTYLQG QIEQTLEKKA GRQYGPPGKV QLIYFIDDLN MPQLDAYDTQ TAIALLRQLA DYGHFYDVSK LALK DIINT QVLAAMNPSA GSFFVNPRYQ RHFWTISIPF PDNESLSLIY ITFLNGHLKR FKSTIQEYSN IIVRASLMLH QAVTQ NFRK TAINFHYEFN LRHMSNVFQG LLLSDPNKFT EPDKLIKLWI HECERTYGDR LVSTDNLKTY KENIFDIVKK SFSKFN FSR YFGNNPENLI YCNFIAGINS DRFYDQMPNN EMEKHISEAL KEYNDNNAFM GLVLFEDAMK HVCRICRIVL PSSGHAL LV GVGGSGKQSL SKLASFIMGY TTFSITISAT YSMVDLRNDL QQLYFKCGPK EEGILFLFTE GQITNERFLV YINDLLSS G EIAELYTLDE KEAMINQVRA KVKGEGKPDT RENCWNWFID QVKKNLHMAI CFSPVGDMRR RARQFPALVN CTVIDWFQP WPYEALFNVA KSFLEPVDLG DDKVREAVVK FMPFSFTLVN DLGLKLLEQE RRYAYTTPKS FLELISLFTN MLAQKRESLE RNKERYETG LVKLKETAEQ VAIIEVEVKE KQVEAEAKKK EADAFAEVVG REKDKVEKEN SKATIEADKC GLIKQNVEAQ K SSTQQDLD AAQPLVEQAK SALNSISKKD FQQAKSFASP PAGVPEVFAA TIYLLAGYFN EAIEIDKNKK PKDVSWKSSL KL MKSPEEF MEKLLNFKDV VDANQVANVN IVKNQYLNMP SFTPEQMASK SAAAKGICSW VVNIVKYYDV IQDVEPKRKA LKE ATEQLE EATVKLNEVE EVVRKLNEEL NKLKAENDKA IAERNAAISE AERCARRLNL AQRLVTALSS ENERWGKSII QLED QLKLM VGDVLVASSF VSYSGPFNKK FRNIMINQNF MKFMKEHTIP MSPDPNPIKI LTDESTIALW NKQKLPSDSV SIENG TILT NSARYPLMID PQLQGITWIR EKEKANNLKI LRLGSKNINR DLELSIENGY SAIIENMNER IDAILMPIIA RSFIKR GKN KIIKFAGKDL ILHPNFKLFL HTKLSNPHYP PEIQAEAALI NFTVTEAGLG DQLLSLVVAR ERPDLAKMKI ELITQQN DF KIKLKDLEDE LLYKLANAKG DILDDIELIE NLEYSKKLSV EIAEKVAAAK ITEAKINETS ENYRPAASRG ALFYFLLS D LSKVHSFYKY SLESFIVVIN RAIDAISENK IYGKTTMMSP RSLKKRVDEL IESLTYTAYQ TTRRGLFESH KLIVAAMLC LRVLLRSEEL NSDEVDHLII GKVDVNPTPM PDALKSFLND NIWAACKALE TIHQFQGFCQ SLETDVLQWK KWYSEEKAET ADLPKAFKE LSKFHRLLLL RALRPDRLPS ALSQFVHDKM GERYIEQPPF NIFETFQETS KTVPIFFVLF PGVDPTPDVE R VAATFDVS ANNGRFINIS MGQGQEDRAK KALFDCAQKG HWIMLQNVHL MQSWLYGLNG LEGFLESVFA SPKTHPNFRV FI SSEPPNV LLPLMQIIPE SILQGSLKIA NEAPQYLKAN LRRAYNKFDQ EFLDKCDKKP TEFKSCLFAL CFFHSLMLGR KKF GTQGWS RVYNFNDGDL TICADVLYNY LSKYDQVPWD DLRYIFGEIM YGGHITDDWD RRTNRTYLKV LIRSELLQQN FNLA PQFKS PDPSKFDYEA YRKYIEEKLP IESPQMFGMH PNAEIGYLTQ TCDQVFNTIL EVQGGSSGGG ASKKDDGVMV TLTDF KTRC PHDFNMLLIE EKVKEKTPYI VVCLQECERM NGLLKEIKTS LEDLRLGLTG ALNMTDAMES LQQSLSFNKV PDTWEK KAY FSKKPLSSWF ADLIERNIQL QEWCKELVTP TSLCISYLFN PMSYLTAIMQ FTARAQGLPL DGITIQTNVT AMKGPED VV NPAENGAYIH GLFLEGAAWE IGGQGQDGYL IEQKPKELHP KMPVINAVAV PLDKKKKNGQ YDCPTYVTSA RGQTFVFT A NLNMESDDSD PNKWILSGTC MLMSDD

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Macromolecule #4: Dynein intermediate chain 2

MacromoleculeName: Dynein intermediate chain 2 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 69.51482 KDa
SequenceString: LTAQELNEDM PSKMLEPKNP QAPKNITVYD YYTRKFKTDE LVDQMIVHFS MDGDYIWKES NEYKTQEEIR DTKKALIKEA MRKQESEEP GANHDEEAIK QTLRNKFNYN TRECQTINPS IRERGVSTEP PPSDTICGNI TQWEIFDAYY AEIMKDHQIE N KKKKEVDQ ...String:
LTAQELNEDM PSKMLEPKNP QAPKNITVYD YYTRKFKTDE LVDQMIVHFS MDGDYIWKES NEYKTQEEIR DTKKALIKEA MRKQESEEP GANHDEEAIK QTLRNKFNYN TRECQTINPS IRERGVSTEP PPSDTICGNI TQWEIFDAYY AEIMKDHQIE N KKKKEVDQ DKKQDQSMYS TSFKRCCKIM ERMVVQNDQE DKYHDYRYYW SQGDNLEAGK NEGHLLPIWR FSNEKQRKKN VT SICWNPL YPDLFAVSLG SYDFTKQRMG LICLYSLKNT THPEYAFNCE AGVMCLDFHP KSAALLAVGL YDGTVLVYDI RNK HKKPIY QSTVRNQKHT DPVWQVKWNP DTSKNYNFYS ISSDGRVMNW ILMKNKLEPE EVILLRLVGK NEEESTLIGL ACGL CFDFN KFEPHIFLVG TEEGKIHKCS RAYSGQYQET YNGHLLAVYK VKWNNFHPRT FISASADWTV RIWDSKYTSQ IICFD LSMM VVDAVWAPYS STVFACATMD KVQVYDLNVD KLNKLAEQKI VKQPKLTNLS FNYKDPILLV GDSHGGVTLV KLSPNL CKS GPEIKQTEDK KAMEEFKNVK IEDYEREKME NL

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Macromolecule #5: Flagellar outer dynein arm intermediate protein, putative

MacromoleculeName: Flagellar outer dynein arm intermediate protein, putative
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 63.702582 KDa
SequenceString: KEFNNPINFQ DTETRYGGIQ NQVVNINQYV QRNPNFIDLD NIAELSEHSV NTERVKTGDR GMSHKEGGWP GNVDPNEAQE TGRFKKRIE KDTSFPQAVK DLKEGVEKCI YQNNQIDLLE EYFEGETSEH VVENLSSKTL MLFKDEKEIC KRSVSEISWH P EGPTKVAV ...String:
KEFNNPINFQ DTETRYGGIQ NQVVNINQYV QRNPNFIDLD NIAELSEHSV NTERVKTGDR GMSHKEGGWP GNVDPNEAQE TGRFKKRIE KDTSFPQAVK DLKEGVEKCI YQNNQIDLLE EYFEGETSEH VVENLSSKTL MLFKDEKEIC KRSVSEISWH P EGPTKVAV SYAIMRFQQM PEKMPTQAYV WDLLNPNSPE IKLMSPSAVT NISYNQKIPD QIGGGCYNGL LAVWDGRKGE NP IMISPVE NSHYEPVTHF HWLMSKTGSE CVTTSTDGKV MWWDTRKFEA GPVEKLNIIE GLGENEEIIG GTALEYNVEA GPS KFLIGT ESGSILTANK KLKKPVEITT RYGLDQGRHL GPVYSINRSN QNPKYFLSVG DWSCKIWVED LKTPIIRTKY HGSY LSDGC WSPTRSGAFF LVRRDGWMDV WDYYYRQNEI AFSHKVSDSP LTCIKINQTG GAYHNSGKLC AIGDQDGTVT ILELC DSLY TMQPKEKDII NEMFEREYRK EKNLETIKKQ QELAKRQVQK DMGSQKEKWE KKKLEMIETA EASFHENLAK NPV

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Macromolecule #6: Dynein light chain roadblock-type 2 protein

MacromoleculeName: Dynein light chain roadblock-type 2 protein / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 14.193171 KDa
SequenceString:
NADDQLKQLS ALEGANGYVI FNESGIPLKR HEKSISHEKA VHIAALVSDL WNVSKKVIQR DLKTPENDIE VIRLRTKHSY EYIITQSGD FTMLAIQLCG KAIEEAKKAA AAAAAAAVVQ EENKEKEKK

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Macromolecule #7: Dynein light chain roadblock-type 2 protein

MacromoleculeName: Dynein light chain roadblock-type 2 protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 17.470111 KDa
SequenceString:
IYKTSKEIQK SDQRIIQQRV ENSIINNQIY QKSLKRKTSK RKVRHQANKK NFQKEMSEVE DTLNRIKTHK TVLGYLIVNS EGGVVRGAF KDEEESKNIA NSIPLLTKKA RSVVRDLDPT NDLVFLRIQT KLNEIMVAPD DEFSLIVIQT KG

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Macromolecule #8: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 10.649161 KDa
SequenceString:
SHLDKVQPVI KNSDMSVEMQ KEVEEVAKKA IDYCNTDKEI ATFIKDDFRS RYHGTWHCIV GRNFGSFVTF ERSYYIYLYV GQLAILLFK TG

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Macromolecule #9: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 11.830534 KDa
SequenceString:
KAVTDMDINE LRKLMIGKAI INSSDMQGDL LQEAQDVIQS GIENNSAPVL NIEAACKYIK ENLDKKFGPT WQCIIGEGYA YDVTVQNNT LLFMFYNGNL AVLIFKS

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Macromolecule #10: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 11.435072 KDa
SequenceString:
QQYKTFMGAR VLWPPECADD ILEGAIRETQ DALKKFEIAR EGQKIAEHLK KYMDDHFDPY WHVFFGKNFG CQAVHNKNRF IYFYIEKTA FLMYQT

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Macromolecule #11: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 10.670071 KDa
SequenceString:
HANEQIIDMP ENSEMKSMKN DAFSQAKFAV ENYKFENKIS SHIKKFFDEK YGPNWHCVVG KHFNAYVSYD SKNFIFFYEG QLAILLYRK G

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Macromolecule #12: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 12.516457 KDa
SequenceString:
MSDSDSDEGR VVEEPLPPHI IRFNDMAQHL LKKVIRQADV LIKENPQGLE KDIALNLVKF VKSQPEFKIG DGEWQCIIGK NFGCSLTFD ANVLAFFDLL PSRKSILLFK SG

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Macromolecule #13: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 10.453167 KDa
SequenceString:
MNHEPEVKAT DMEEDMIKRV KEIAINAVKE YKQEKQIAHY IKYEFDKIDG YGWNCIVGRN FGSHIIHQTK KYIFFKINEL CLLLWKA

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Macromolecule #14: Dynein light chain tctex-type 1 protein

MacromoleculeName: Dynein light chain tctex-type 1 protein / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 12.856455 KDa
SequenceString:
TDKEYISEEV QKAIDDSVKQ VFGIKDDSSQ VTITYNKDKV NLWTQQIIDY TIRGLNKLGK HFKYCVTAIL QQTNHAGISV QITAYQDTA TDGSLIQCYE INDIYAIVSV FAMAV

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Macromolecule #15: Dynein light chain 2A

MacromoleculeName: Dynein light chain 2A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 14.117313 KDa
SequenceString:
RKREASLITL NYIKNRFYPS KIQKIIKELF EDRLKGVEYD PNNANQLSER LVLELREKIK RGKVPRYKIG VQVVFGEIKG QGLRIASKC LWDVQNDNYA SYTYTSEKVY CTGIVFGCYF E

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Macromolecule #16: Thioredoxin

MacromoleculeName: Thioredoxin / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 12.987904 KDa
SequenceString:
TTTVTSEDHF MTFYNENNKK LIVVDVYPGW SGPCTAMYPT YNQLMISIDD FEKRIDILLL DQDKLVTYKN DKFHATCQPK FLFISEGKI IDEVLGTNIP VFIEKVNKYI PLS

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Macromolecule #17: Dynein light chain 1

MacromoleculeName: Dynein light chain 1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 21.705334 KDa
SequenceString: AKGTTCQKAI VNWEAANPGK NPSEAEEIKL IFQIPPIEKM DGPVLNTLTK CKKLSLSSNS IDKMISLNML RNLEILSLSR NVIKKISGL EDIGGTLRQL WLSYNFIEKL DGLNNCSVLQ TLYIGNNRIK NWEELDKLKD LPELENVLFY GNPIYEQVKE D PKLIVLKK ...String:
AKGTTCQKAI VNWEAANPGK NPSEAEEIKL IFQIPPIEKM DGPVLNTLTK CKKLSLSSNS IDKMISLNML RNLEILSLSR NVIKKISGL EDIGGTLRQL WLSYNFIEKL DGLNNCSVLQ TLYIGNNRIK NWEELDKLKD LPELENVLFY GNPIYEQVKE D PKLIVLKK LPTLKNVDGM IIDDSVLEKV KQIA

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Macromolecule #18: Dynein light chain 4A

MacromoleculeName: Dynein light chain 4A / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 16.803703 KDa
SequenceString:
MASEVMNLSE EDIQFCQKAF SDYDEDGLGA IKVSDLKLAL ENIGCSLSEN DLFKMISEVD EKNTGLIKFS DFLNIYYKQK YANLGDDDQ DLVDAFVAMG GNADTTGSVD ANRLIQIVKE EFEMTIDIVG LIREIDKDGS GEIEYEEFKL L

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Macromolecule #19: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 19 / Number of copies: 6 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

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Macromolecule #20: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 20 / Number of copies: 3 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

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Macromolecule #21: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 21 / Number of copies: 9 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 53.3 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 76936

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