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- EMDB-19040: Structure of Integrator-PP2A-SOSS-CTD post-termination complex -

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Basic information

Entry
Database: EMDB / ID: EMD-19040
TitleStructure of Integrator-PP2A-SOSS-CTD post-termination complex
Map dataOverall map filtered by local resolution
Sample
  • Complex: Post-termination complex
    • Complex: Integrator-PP2A complex and NABP2 and INIP
      • Protein or peptide: x 2 types
    • Complex: RNA Polymerase II C-terminal domain peptides
      • Protein or peptide: x 18 types
    • Complex: DSS1
      • Protein or peptide: x 1 types
  • Ligand: x 2 types
KeywordsIntegrator complex / Post-termination complex / RNA Polymerase II / Pol II / RNAPII / TRANSCRIPTION
Function / homology
Function and homology information


regulation of fertilization / SOSS complex / U2 snRNA 3'-end processing / snRNA processing / integrator complex / snRNA 3'-end processing / meiotic spindle elongation / Integration of energy metabolism / PP2A-mediated dephosphorylation of key metabolic factors / regulation of microtubule binding ...regulation of fertilization / SOSS complex / U2 snRNA 3'-end processing / snRNA processing / integrator complex / snRNA 3'-end processing / meiotic spindle elongation / Integration of energy metabolism / PP2A-mediated dephosphorylation of key metabolic factors / regulation of microtubule binding / MASTL Facilitates Mitotic Progression / mitotic sister chromatid separation / regulation of meiotic cell cycle process involved in oocyte maturation / protein phosphatase type 2A complex / flagellated sperm motility / establishment of protein localization to telomere / meiotic sister chromatid cohesion, centromeric / peptidyl-serine dephosphorylation / protein localization to nuclear envelope / regulation of transcription elongation by RNA polymerase II / peptidyl-threonine dephosphorylation / : / positive regulation of microtubule binding / negative regulation of tyrosine phosphorylation of STAT protein / Regulation of glycolysis by fructose 2,6-bisphosphate metabolism / Inhibition of replication initiation of damaged DNA by RB1/E2F1 / female meiotic nuclear division / protein antigen binding / protein phosphatase regulator activity / ceramide metabolic process / GABA receptor binding / negative regulation of epithelial to mesenchymal transition / G-rich strand telomeric DNA binding / APC truncation mutants have impaired AXIN binding / AXIN missense mutants destabilize the destruction complex / Truncations of AMER1 destabilize the destruction complex / Initiation of Nuclear Envelope (NE) Reformation / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Major Pathway / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / ERKs are inactivated / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / positive regulation of extrinsic apoptotic signaling pathway in absence of ligand / Beta-catenin phosphorylation cascade / Signaling by GSK3beta mutants / CTNNB1 S33 mutants aren't phosphorylated / CTNNB1 S37 mutants aren't phosphorylated / CTNNB1 S45 mutants aren't phosphorylated / CTNNB1 T41 mutants aren't phosphorylated / Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters / mitotic G2/M transition checkpoint / regulation of Wnt signaling pathway / Disassembly of the destruction complex and recruitment of AXIN to the membrane / regulation of growth / centrosome localization / inner cell mass cell proliferation / negative regulation of glycolytic process through fructose-6-phosphate / positive regulation of NLRP3 inflammasome complex assembly / myosin phosphatase activity / response to ionizing radiation / protein serine/threonine phosphatase activity / CTLA4 inhibitory signaling / Platelet sensitization by LDL / negative regulation of MAPK cascade / protein-serine/threonine phosphatase / regulation of cell differentiation / T cell homeostasis / ERK/MAPK targets / regulation of G1/S transition of mitotic cell cycle / regulation of DNA replication / positive regulation of telomere capping / phosphoprotein phosphatase activity / mesoderm development / RNA polymerase II transcribes snRNA genes / chromosome, centromeric region / DARPP-32 events / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / lateral plasma membrane / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / RNA polymerase II, core complex / DNA polymerase binding / regulation of cell adhesion
Similarity search - Function
SOSS complex subunit C / SOSS complex subunit C / Integrator complex subunit 10 / Integrator complex subunit 15 / Integrator complex subunit 15 / Integrator subunit 10 / Cell cycle regulator Mat89Bb / Integrator complex subunit 14 / Integrator complex subunit 14, beta-barrel domain / Integrator complex subunit 14, C-terminal ...SOSS complex subunit C / SOSS complex subunit C / Integrator complex subunit 10 / Integrator complex subunit 15 / Integrator complex subunit 15 / Integrator subunit 10 / Cell cycle regulator Mat89Bb / Integrator complex subunit 14 / Integrator complex subunit 14, beta-barrel domain / Integrator complex subunit 14, C-terminal / Cell cycle and development regulator Mat89Bb / Integrator complex subunit 14, beta-barrel domain / Integrator complex subunit 14, alpha-helical domain / Integrator complex subunit 3, N-terminal / Integrator complex subunit 3 / Integrator complex subunit 3 N-terminal / : / Single-stranded DNA binding protein Ssb-like, OB fold / Domain of unknown function DUF3677 / Integrator complex subunit 2, metazoa / Integrator complex subunit 2 / Integrator complex subunit 5, C-terminal / Integrator complex subunit 5, N-terminal / Integrator complex subunit 7 / Integrator complex subunit 8 / Integrator complex subunit 1 / Integrator complex subunit 5 / Protein of unknown function (DUF3677) / Integrator complex subunit 2 / Integrator complex subunit 5 N-terminus / Integrator complex subunit 5 C-terminus / INTS6/SAGE1/DDX26B/CT45, C-terminal / INTS6/SAGE1/DDX26B/CT45 C-terminus / : / : / Integrator IntS9, C-terminal domain / Integrator IntS11, C-terminal domain / Integrator complex subunit 9 / Integrator complex subunit 11, MBL-fold / Sister chromatid cohesion protein PDS5 protein / Metallo-beta-lactamase superfamily domain / : / Beta-Casp domain / Beta-Casp domain / Beta-Casp domain / Zn-dependent metallo-hydrolase, RNA specificity domain / Zn-dependent metallo-hydrolase RNA specificity domain / HEAT repeat / HEAT repeat / Serine/threonine specific protein phosphatases signature. / Protein phosphatase 2A homologues, catalytic domain. / Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase / HEAT repeat profile. / HEAT, type 2 / von Willebrand factor type A domain / HEAT repeats / Calcineurin-like phosphoesterase domain, ApaH type / Calcineurin-like phosphoesterase / RNA polymerase II, heptapeptide repeat, eukaryotic / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1 C-terminal repeat / Eukaryotic RNA polymerase II heptapeptide repeat. / Metallo-dependent phosphatase-like / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / VWFA domain profile. / Metallo-beta-lactamase superfamily / Metallo-beta-lactamase / von Willebrand factor, type A / von Willebrand factor A-like domain superfamily / Ribonuclease Z/Hydroxyacylglutathione hydrolase-like / RNA polymerase Rpb1, domain 3 superfamily / RNA polymerase Rpb1, clamp domain superfamily / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 4 / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase I subunit A N-terminus / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 5 / Armadillo-like helical / Tetratricopeptide-like helical domain superfamily / Armadillo-type fold / Nucleic acid-binding, OB-fold
Similarity search - Domain/homology
DNA-directed RNA polymerase subunit / Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform / Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform / Integrator complex subunit 11 / Integrator complex subunit 3 / Integrator complex subunit 5 / Integrator complex subunit 8 / Integrator complex subunit 1 / Integrator complex subunit 4 / Integrator complex subunit 15 ...DNA-directed RNA polymerase subunit / Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform / Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform / Integrator complex subunit 11 / Integrator complex subunit 3 / Integrator complex subunit 5 / Integrator complex subunit 8 / Integrator complex subunit 1 / Integrator complex subunit 4 / Integrator complex subunit 15 / Integrator complex subunit 14 / SOSS complex subunit B1 / Integrator complex subunit 2 / SOSS complex subunit C / Integrator complex subunit 9 / Integrator complex subunit 7 / Integrator complex subunit 13 / Integrator complex subunit 10 / Integrator complex subunit 6
Similarity search - Component
Biological speciesHomo sapiens (human) / Sus scrofa (pig) / Trichoplusia ni (cabbage looper)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.7 Å
AuthorsFianu I / Ochmann M / Walshe JL / Cramer P
Funding support Germany, European Union, 3 items
OrganizationGrant numberCountry
German Research Foundation (DFG)SFB860 Germany
German Research Foundation (DFG)SFB1565 Germany
European Research Council (ERC)EXC 2067/1-390729940European Union
CitationJournal: Nature / Year: 2024
Title: Structural basis of Integrator-dependent RNA polymerase II termination.
Authors: Isaac Fianu / Moritz Ochmann / James L Walshe / Olexandr Dybkov / Joseph Neos Cruz / Henning Urlaub / Patrick Cramer /
Abstract: The Integrator complex can terminate RNA polymerase II (Pol II) in the promoter-proximal region of genes. Previous work has shed light on how Integrator binds to the paused elongation complex ...The Integrator complex can terminate RNA polymerase II (Pol II) in the promoter-proximal region of genes. Previous work has shed light on how Integrator binds to the paused elongation complex consisting of Pol II, the DRB sensitivity-inducing factor (DSIF) and the negative elongation factor (NELF) and how it cleaves the nascent RNA transcript, but has not explained how Integrator removes Pol II from the DNA template. Here we present three cryo-electron microscopy structures of the complete Integrator-PP2A complex in different functional states. The structure of the pre-termination complex reveals a previously unresolved, scorpion-tail-shaped INTS10-INTS13-INTS14-INTS15 module that may use its 'sting' to open the DSIF DNA clamp and facilitate termination. The structure of the post-termination complex shows that the previously unresolved subunit INTS3 and associated sensor of single-stranded DNA complex (SOSS) factors prevent Pol II rebinding to Integrator after termination. The structure of the free Integrator-PP2A complex in an inactive closed conformation reveals that INTS6 blocks the PP2A phosphatase active site. These results lead to a model for how Integrator terminates Pol II transcription in three steps that involve major rearrangements.
History
DepositionDec 5, 2023-
Header (metadata) releaseFeb 7, 2024-
Map releaseFeb 7, 2024-
UpdateApr 10, 2024-
Current statusApr 10, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_19040.png

Downloads & links

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Map

FileDownload / File: emd_19040.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationOverall map filtered by local resolution
Voxel sizeX=Y=Z: 1.05 Å
Density
Contour LevelBy AUTHOR: 0.00308
Minimum - Maximum-0.08337648 - 0.19503137
Average (Standard dev.)0.00030226208 (±0.0026171028)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 503.99997 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Focused refined map around Integrator cleavage module (INTS4,...

Fileemd_19040_additional_1.map
AnnotationFocused refined map around Integrator cleavage module (INTS4, INTS9 and INTS11)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Focused refined map around INTS1, INTS2 INTS7 and DSS1

Fileemd_19040_additional_2.map
AnnotationFocused refined map around INTS1, INTS2 INTS7 and DSS1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Additional map: Focused refined map around INTS5, INTS8 and INTS15

Fileemd_19040_additional_3.map
AnnotationFocused refined map around INTS5, INTS8 and INTS15
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Focused refined map around INTS6 and PP2A

Fileemd_19040_additional_4.map
AnnotationFocused refined map around INTS6 and PP2A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Focused refined map around INTS3 and SOSS factors

Fileemd_19040_additional_5.map
AnnotationFocused refined map around INTS3 and SOSS factors
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Additional map: Focused refined map around INTS1 c-terminus and center of Integrator

Fileemd_19040_additional_6.map
AnnotationFocused refined map around INTS1 c-terminus and center of Integrator
Projections & Slices
AxesZYX

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Slices (1/2)
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Histogram

Histogram (log scale)

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Half map: #1

Fileemd_19040_half_map_1.map
Projections & Slices
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Histogram

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Half map: #2

Fileemd_19040_half_map_2.map
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Sample components

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Entire : Post-termination complex

EntireName: Post-termination complex
Components
  • Complex: Post-termination complex
    • Complex: Integrator-PP2A complex and NABP2 and INIP
      • Protein or peptide: UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK
      • Protein or peptide: DNA-directed RNA polymerase subunitPolymerase
    • Complex: RNA Polymerase II C-terminal domain peptides
      • Protein or peptide: SOSS complex subunit B1Southern Okanagan Secondary School
      • Protein or peptide: SOSS complex subunit CSouthern Okanagan Secondary School
      • Protein or peptide: Integrator complex subunit 1
      • Protein or peptide: Integrator complex subunit 2
      • Protein or peptide: Integrator complex subunit 3
      • Protein or peptide: Integrator complex subunit 4
      • Protein or peptide: Integrator complex subunit 5
      • Protein or peptide: Integrator complex subunit 6
      • Protein or peptide: Integrator complex subunit 7
      • Protein or peptide: Integrator complex subunit 8
      • Protein or peptide: Integrator complex subunit 9
      • Protein or peptide: Integrator complex subunit 10
      • Protein or peptide: Integrator complex subunit 11
      • Protein or peptide: Integrator complex subunit 14
      • Protein or peptide: Integrator complex subunit 15
      • Protein or peptide: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
      • Protein or peptide: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
      • Protein or peptide: Integrator complex subunit 13
    • Complex: DSS1
      • Protein or peptide: DSS1
  • Ligand: ZINC ION
  • Ligand: MANGANESE (II) ION

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Supramolecule #1: Post-termination complex

SupramoleculeName: Post-termination complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#21
Details: Integrator-PP2A, RNA Polymerase II C-terminal domain peptides, NABP2, INIP
Molecular weightTheoretical: 1.2 MDa

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Supramolecule #2: Integrator-PP2A complex and NABP2 and INIP

SupramoleculeName: Integrator-PP2A complex and NABP2 and INIP / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1, #4
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #3: RNA Polymerase II C-terminal domain peptides

SupramoleculeName: RNA Polymerase II C-terminal domain peptides / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2-#3, #5-#19, #21
Source (natural)Organism: Sus scrofa (pig)

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Supramolecule #4: DSS1

SupramoleculeName: DSS1 / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #20
Source (natural)Organism: Trichoplusia ni (cabbage looper)

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Macromolecule #1: UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK

MacromoleculeName: UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 783.958 Da
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #2: SOSS complex subunit B1

MacromoleculeName: SOSS complex subunit B1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.566053 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMTTETFVK DIKPGLKNLN LIFIVLETGR VTKTKDGHEV RTCKVADKTG SINISVWDDV GNLIQPGDII RLTKGYASVF KGCLTLYTG RGGDLQKIGE FCMVYSEVPN FSEPNPEYST QQAPNKAVQN DSNPSASQPT TGPSAASPAS ENQNGNGLSA P PGPGGGPH ...String:
SNMTTETFVK DIKPGLKNLN LIFIVLETGR VTKTKDGHEV RTCKVADKTG SINISVWDDV GNLIQPGDII RLTKGYASVF KGCLTLYTG RGGDLQKIGE FCMVYSEVPN FSEPNPEYST QQAPNKAVQN DSNPSASQPT TGPSAASPAS ENQNGNGLSA P PGPGGGPH PPHTPSHPPS TRITRSQPNH TPAGPPGPSS NPVSNGKETR RSSKR

UniProtKB: SOSS complex subunit B1

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Macromolecule #3: SOSS complex subunit C

MacromoleculeName: SOSS complex subunit C / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 11.642977 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString:
SNMAANSSGQ GFQNKNRVAI LAELDKEKRK LLMQNQSSTN HPGASIALSR PSLNKDFRDH AEQQHIAAQQ KAALQHAHAH SSGYFITQD SAFGNLILPV LPRLDPE

UniProtKB: SOSS complex subunit C

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Macromolecule #4: DNA-directed RNA polymerase subunit

MacromoleculeName: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 1.356393 KDa
SequenceString:
SPSYSPTSPS YSP

UniProtKB: DNA-directed RNA polymerase subunit

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Macromolecule #5: Integrator complex subunit 1

MacromoleculeName: Integrator complex subunit 1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 244.776078 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMNRAKPTT VRRPSAAAKP SGHPPPGDFI ALGSKGQANE SKTASTLLKP APSGLPSERK RDAAAALSSA SALTGLTKRP KLSSTPPLS ALGRLAEAAV AEKRAISPSI KEPSVVPIEV LPTVLLDEIE AAELEGNDDR IEGVLCGAVK QLKVTRAKPD S TLYLSLMY ...String:
SNMNRAKPTT VRRPSAAAKP SGHPPPGDFI ALGSKGQANE SKTASTLLKP APSGLPSERK RDAAAALSSA SALTGLTKRP KLSSTPPLS ALGRLAEAAV AEKRAISPSI KEPSVVPIEV LPTVLLDEIE AAELEGNDDR IEGVLCGAVK QLKVTRAKPD S TLYLSLMY LAKIKPNIFA TEGVIEALCS LLRRDASINF KAKGNSLVSV LACNLLMAAY EEDENWPEIF VKVYIEDSLG ER IWVDSPH CKTFVDNIQT AFNTRMPPRS VLLQGEAGRV AGDLGAGSSP HPSLTEEEDS QTELLIAEEK LSPEQEGQLM PRY EELAES VEEYVLDMLR DQLNRRQPID NVSRNLLRLL TSTCGYKEVR LLAVQKLEMW LQNPKLTRPA QDLLMSVCMN CNTH GSEDM DVISHLIKIR LKPKVLLNHF MLCIRELLSA HKDNLGTTIK LVIFNELSSA RNPNNMQVLY TALQHSSELA PKFLA MVFQ DLLTNKDDYL RASRALLREI IKQTKHEINF QAFCLGLMQE RKEPQYLEME FKERFVVHIT DVLAVSMMLG ITAQVK EAG IAWDKGEKRN LEVLRSFQNQ IAAIQRDAVW WLHTVVPSIS KLAPKDYVHC LHKVLFTEQP ETYYKWDNWP PESDRNF FL RLCSEVPILE DTLMRILVIG LSRELPLGPA DAMELADHLV KRAAAVQADD VEVLKVGRTQ LIDAVLNLCT YHHPENIQ L PPGYQPPNLA ISTLYWKAWP LLLVVAAFNP ENIGLAAWEE YPTLKMLMEM VMTNNYSYPP CTLTDEETRT EMLNRELQT AQREKQEILA FEGHLAAAST KQTITESSSL LLSQLTSLDP QGPPRRPPPH ILDQVKSLNQ SLRLGHLLCR SRNPDFLLHI IQRQASSQS MPWLADLVQS SEGSLDVLPV QCLCEFLLHD AVDDAASGEE DDEGESKEQK AKKRQRQQKQ RQLLGRLQDL L LGPKADEQ TTCEVLDYFL RRLGSSQVAS RVLAMKGLSL VLSEGSLRDG EEKEPPMEED VGDTDVLQGY QWLLRDLPRL PL FDSVRST TALALQQAIH METDPQTISA YLIYLSQHTP VEEQAQHSDL ALDVARLVVE RSTIMSHLFS KLSPSAASDA VLS ALLSIF SRYVRRMRQS KEGEEVYSWS ESQDQVFLRW SSGETATMHI LVVHAMVILL TLGPPRADDS EFQALLDIWF PEEK PLPTA FLVDTSEEAL LLPDWLKLRM IRSEVLRLVD AALQDLEPQQ LLLFVQSFGI PVSSMSKLLQ FLDQAVAHDP QTLEQ NIMD KNYMAHLVEV QHERGASGGQ TFHSLLTASL PPRRDSTEAP KPKSSPEQPI GQGRIRVGTQ LRVLGPEDDL AGMFLQ IFP LSPDPRWQSS SPRPVALALQ QALGQELARV VQGSPEVPGI TVRVLQALAT LLSSPHGGAL VMSMHRSHFL ACPLLRQ LC QYQRCVPQDT GFSSLFLKVL LQMLQWLDSP GVEGGPLRAQ LRMLASQASA GRRLSDVRGG LLRLAEALAF RQDLEVVS S TVRAVIATLR SGEQCSVEPD LISKVLQGLI EVRSPHLEEL LTAFFSATAD AASPFPACKP VVVVSSLLLQ EEEPLAGGK PGADGGSLEA VRLGPSSGLL VDWLEMLDPE VVSSCPDLQL RLLFSRRKGK GQAQVPSFRP YLLTLFTHQS SWPTLHQCIR VLLGKSREQ RFDPSASLDF LWACIHVPRI WQGRDQRTPQ KRREELVLRV QGPELISLVE LILAEAETRS QDGDTAACSL I QARLPLLL SCCCGDDESV RKVTEHLSGC IQQWGDSVLG RRCRDLLLQL YLQRPELRVP VPEVLLHSEG AASSSVCKLD GL IHRFITL LADTSDSRAL ENRGADASMA CRKLAVAHPL LLLRHLPMIA ALLHGRTHLN FQEFRQQNHL SCFLHVLGLL ELL QPHVFR SEHQGALWDC LLSFIRLLLN YRKSSRHLAA FINKFVQFIH KYITYNAPAA ISFLQKHADP LHDLSFDNSD LVML KSLLA GLSLPSRDDR TDRGLDEEGE EESSAGSLPL VSVSLFTPLT AAEMAPYMKR LSRGQTVEDL LEVLSDIDEM SRRRP EILS FFSTNLQRLM SSAEECCRNL AFSLALRSMQ NSPSIAAAFL PTFMYCLGSQ DFEVVQTALR NLPEYALLCQ EHAAVL LHR AFLVGMYGQM DPSAQISEAL RILHMEAVM

UniProtKB: Integrator complex subunit 1

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Macromolecule #6: Integrator complex subunit 2

MacromoleculeName: Integrator complex subunit 2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 134.451625 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MKDQQTVIMT ECTSLQFVSP FAFEAMQKVD VVCLASLSDP ELRLLLPCLV RMALCAPADQ SQSWAQDKKL ILRLLSGVEA VNSIVALLS VDFHALEQDA SKEQQLRHKL GGGSGESILV SQLQHGLTLE FEHSDSPRRL RLVLSELLAI MNKVSESNGE F FFKSSELF ...String:
MKDQQTVIMT ECTSLQFVSP FAFEAMQKVD VVCLASLSDP ELRLLLPCLV RMALCAPADQ SQSWAQDKKL ILRLLSGVEA VNSIVALLS VDFHALEQDA SKEQQLRHKL GGGSGESILV SQLQHGLTLE FEHSDSPRRL RLVLSELLAI MNKVSESNGE F FFKSSELF ESPVYLEEAA DVLCILQAEL PSLLPIVDVA EALLHVRNGA WFLCLLVANV PDSFNEVCRG LIKNGERQDE ES LGGRRRT DALRFLCKMN PSQALKVRGM VVEECHLPGL GVALTLDHTK NEACEDGVSD LVCFVSGLLL GTNAKVRTWF GTF IRNGQQ RKRETSSSVL WQMRRQLLLE LMGILPTVRS TRIVEEADVD MEPNVSVYSG LKEEHVVKAS ALLRLYCALM GIAG LKPTE EEAEQLLQLM TSRPPATPAG VRFVSLSFCM LLAFSTLVST PEQEQLMVVW LSWMIKEEAY FESTSGVSAS FGEML LLVA MYFHSNQLSA IIDLVCSTLG MKIVIKPSSL SRMKTIFTQE IFTEQVVTAH AVRVPVTSNL SANITGFLPI HCIYQL LRS RSFTKHKVSI KDWIYRQLCE TSTPLHPQLL PLIDVYINSI LTPASKSNPE ATNQPVTEQE ILNIFQGVIG GDNIRLN QR FSITAQLLVL YYILSYEEAL LANTKTLAAM QRKPKSYSSS LMDQIPIKFL IRQAQGLQQE LGGLHSALLR LLATNYPH L CIVDDWICEE EITGTDALLR RMLLTNNAKN HSPKQLQEAF SAVPVNNTQV MQIIEHLTLL SASELIPYAE VLTSNMSQL LNSGVPRRIL QTVNKLWMVL NTVMPRRLWV MTVNALQPSI KFVRQQKYTQ NDLMIDPLIV LRCDQRVHRC PPLMDITLHM LNGYLLASK AYLSAHLKET EQDRPSQNNT IGLVGQTDAP EVTREELKNA LLAAQDSAAV QILLEICLPT EEEKANGVNP D SLLRNVQS VITTSAPNKG MEEGEDNLLC NLREVQCLIC CLLHQMYIAD PNIAKLVHFQ GYPCELLPLT VAGIPSMHIC LD FIPELIA QPELEKQIFA IQLLSHLCIQ YALPKSLSVA RLAVNVMGTL LTVLTQAKRY AFFMPTLPSL VSFCRAFPPL YED IMSLLI QIGQVCASDV ATQTRDIDPI ITRLQQIKEK PSGWSQICKD SSYKNGSRDT GSMDPDVQLC HCIERTVIEI INMS VSGI

UniProtKB: Integrator complex subunit 2

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Macromolecule #7: Integrator complex subunit 3

MacromoleculeName: Integrator complex subunit 3 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 118.155555 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MELQKGKGAA AAAAASGAAG GGGGGAGAGA PGGGRLLLST SLDAKDELEE RLERCMSIVT SMTAGVSERE ANDALNAYVC KGLPQHEEI CLGLFTLILT EPAQAQKCYR DLALVSRDGM NIVLNKINQI LMEKYLKLQD TCRTQLVWLV RELVKSGVLG A DGVCMTFM ...String:
MELQKGKGAA AAAAASGAAG GGGGGAGAGA PGGGRLLLST SLDAKDELEE RLERCMSIVT SMTAGVSERE ANDALNAYVC KGLPQHEEI CLGLFTLILT EPAQAQKCYR DLALVSRDGM NIVLNKINQI LMEKYLKLQD TCRTQLVWLV RELVKSGVLG A DGVCMTFM KQIAGGDVTA KNIWLAESVL DILTEQREWV LKSSILIAMA VYTYLRLIVD HHGTAQLQAL RQKEVDFCIS LL RERFMEC LMIGRDLVRL LQNVARIPEF ELLWKDIIHN PQALSPQFTG ILQLLQSRTS RKFLACRLTP DMETKLLFMT SRV RFGQQK RYQDWFQRQY LSTPDSQSLR CDLIRYICGV VHPSNEVLSS DILPRWAIIG WLLTTCTSNV AASNAKLALF YDWL FFSPD KDSIMNIEPA ILVMHHSMKP HPAITATLLD FMCRIIPNFY PPLEGHVRQG VFSSLNHIVE KRVLAHLAPL FDNPK LDKE LRAMLREKFP EFCSSPSPPV EVKIEEPVSM EMDNHMSDKD ESCYDNAEAA FSDDEEDLNS KGKKREFRFH PIKETV VEE PVDITPYLDQ LDESLRDKVL QLQKGSDTEA QCEVMQEIVD QVLEEDFDSE QLSVLASCLQ ELFKAHFRGE VLPEEIT EE SLEESVGKPL YLIFRNLCQM QEDNSSFSLL LDLLSELYQK QPKIGYHLLY YLRASKAAAG KMNLYESFAQ ATQLGDLH T CLMMDMKACQ EDDVRLLCHL TPSIYTEFPD ETLRSGELLN MIVAVIDSAQ LQELVCHVMM GNLVMFRKDS VLNILIQSL DWETFEQYCA WQLFLAHNIP LETIIPILQH LKYKEHPEAL SCLLLQLRRE KPSEEMVKMV LSRPCHPDDQ FTTSILRHWC MKHDELLAE HIKSLLIKNN SLPRKRQSLR SSSSKLAQLT LEQILEHLDN LRLNLTNTKQ NFFSQTPILQ ALQHVQASCD E AHKMKFSD LFSLAEEYED SSTKPPKSRR KAALSSPRSR KNATQPPNAE EESGSSSASE EEDTKPKPTK RKRKGSSAVG SD SD

UniProtKB: Integrator complex subunit 3

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Macromolecule #8: Integrator complex subunit 4

MacromoleculeName: Integrator complex subunit 4 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 108.306758 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAAHLKKRVY EEFTKVVQPQ EEIATKKLRL TKPSKSAALH IDLCKATSPA DALQYLLQFA RKPVEAESVE GVVRILLEHY YKENDPSVR LKIASLLGLL SKTAGFSPDC IMDDAINILQ NEKSHQVLAQ LLDTLLAIGT KLPENQAIQM RLVDVACKHL T DTSHGVRN ...String:
MAAHLKKRVY EEFTKVVQPQ EEIATKKLRL TKPSKSAALH IDLCKATSPA DALQYLLQFA RKPVEAESVE GVVRILLEHY YKENDPSVR LKIASLLGLL SKTAGFSPDC IMDDAINILQ NEKSHQVLAQ LLDTLLAIGT KLPENQAIQM RLVDVACKHL T DTSHGVRN KCLQLLGNLG SLEKSVTKDA EGLAARDVQK IIGDYFSDQD PRVRTAAIKA MLQLHERGLK LHQTIYNQAC KL LSDDYEQ VRSAAVQLIW VVSQLYPESI VPIPSSNEEI RLVDDAFGKI CHMVSDGSWV VRVQAAKLLG SMEQVSSHFL EQT LDKKLM SDLRRKRTAH ERAKELYSSG EFSSGRKWGD DAPKEEVDTG AVNLIESGAC GAFVHGLEDE MYEVRIAAVE ALCM LAQSS PSFAEKCLDF LVDMFNDEIE EVRLQSIHTM RKISNNITLR EDQLDTVLAV LEDSSRDIRE ALHELLCCTN VSTKE GIHL ALVELLKNLT KYPTDRDSIW KCLKFLGSRH PTLVLPLVPE LLSTHPFFDT AEPDMDDPAY IAVLVLIFNA AKTCPT MPA LFSDHTFRHY AYLRDSLSHL VPALRLPGRK LVSSAVSPSI IPQEDPSQQF LQQSLERVYS LQHLDPQGAQ ELLEFTI RD LQRLGELQSE LAGVADFSAT YLRCQLLLIK ALQEKLWNVA APLYLKQSDL ASAAAKQIME ETYKMEFMYS GVENKQVV I IHHMRLQAKA LQLIVTARTT RGLDPLFGMC EKFLQEVDFF QRYFIADLPH LQDSFVDKLL DLMPRLMTSK PAEVVKILQ TMLRQSAFLH LPLPEQIHKA SATIIEPAGE SDNPLRFTSG LVVALDVDAT LEHVQDPQNT VKVQVLYPDG QAQMIHPKPA DFRNPGPGR HRLITQVYLS HTAWTEACQV EVRLLLAYNS SARIPKCPWM EGGEMSPQVE TSIEGTIPFS KPVKVYIMPK P ARR

UniProtKB: Integrator complex subunit 4

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Macromolecule #9: Integrator complex subunit 5

MacromoleculeName: Integrator complex subunit 5 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 108.316422 KDa
Recombinant expressionOrganism: Trichoplusia (butterflies/moths)
SequenceString: SNMSALCDPP GAPGPPGPAP ATHGPAPLSA QELSQEIKAF LTGVDPILGH QLSAREHARC GLLLLRSLPP ARAAVLDHLR GVFDESVRA HLAALDETPV AGPPHLRPPP PSHVPAGGPG LEDVVQEVQQ VLSEFIRANP KAWAPVISAW SIDLMGQLSS T YSGQHQRV ...String:
SNMSALCDPP GAPGPPGPAP ATHGPAPLSA QELSQEIKAF LTGVDPILGH QLSAREHARC GLLLLRSLPP ARAAVLDHLR GVFDESVRA HLAALDETPV AGPPHLRPPP PSHVPAGGPG LEDVVQEVQQ VLSEFIRANP KAWAPVISAW SIDLMGQLSS T YSGQHQRV PHATGALNEL LQLWMGCRAT RTLMDIYVQC LSALIGSCPD ACVDALLDTS VQHSPHFDWV VAHIGSSFPG TI ISRVLSC GLKDFCVHGG AGGGAGSSGG SSSQTPSTDP FPGSPAIPAE KRVPKIASVV GILGHLASRH GDSIRRELLR MFH DSLAGG SGGRSGDPSL QATVPFLLQL AVMSPALLGT VSGELVDCLK PPAVLSQLQQ HLQGFPREEL DNMLNLAVHL VSQA SGAGA YRLLQFLVDT AMPASVITTQ GLAVPDTVRE ACDRLIQLLL LHLQKLVHHR GGSPGEGVLG PPPPPRLVPF LDALK NHVG ELCGETLRLE RKRFLWQHQL LGLLSVYTRP SCGPEALGHL LSRARSPEEL SLATQLYAGL VVSLSGLLPL AFRSCL ARV HAGTLQPPFT ARFLRNLALL VGWEQQGGEG PAALGAHFGE SASAHLSDLA PLLLHPEEEV AEAAASLLAI CPFPSEA LS PSQLLGLVRA GVHRFFASLR LHGPPGVASA CQLLTRLSQT SPAGLKAVLQ LLVEGALHRG NTELFGGQVD GDNETLSV V SASLASASLL DTNRRHTAAV PGPGGIWSVF HAGVIGRGLK PPKFVQSRNQ QEVIYNTQSL LSLLVHCCSA PGGTECGEC WGAPILSPEA AKAVAVTLVE SVCPDAAGAE LAWPPEEHAR ATVERDLRIG RRFREQPLLF ELLKLVAAAP PALCYCSVLL RGLLAALLG HWEASRHPDT THSPWHLEAS CTLVAVMAEG SLLPPALGNM HEVFSQLAPF EVRLLLLSVW GFLREHGPLP Q KFIFQSER GRFIRDFSRE GGGEGGPHLA VLHSVLHRNI DRLGLFSGRF QAPSPSTLLR QGT

UniProtKB: Integrator complex subunit 5

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Macromolecule #10: Integrator complex subunit 6

MacromoleculeName: Integrator complex subunit 6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 100.72825 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMPILLFLI DTSASMNQRS HLGTTYLDTA KGAVETFMKL RARDPASRGD RYMLVTFEEP PYAIKAGWKE NHATFMNELK NLQAEGLTT LGQSLRTAFD LLNLNRLVTG IDNYGQGRNP FFLEPAIIIT ITDGSKLTTT SGVQDELHLP LNSPLPGSEL T KEPFRWDQ ...String:
SNMPILLFLI DTSASMNQRS HLGTTYLDTA KGAVETFMKL RARDPASRGD RYMLVTFEEP PYAIKAGWKE NHATFMNELK NLQAEGLTT LGQSLRTAFD LLNLNRLVTG IDNYGQGRNP FFLEPAIIIT ITDGSKLTTT SGVQDELHLP LNSPLPGSEL T KEPFRWDQ RLFALVLRLP GTMSVESEQL TGVPLDDSAI TPMCEVTGGR SYSVCSPRML NQCLESLVQK VQSGVVINFE KA GPDPSPV EDGQPDISRP FGSQPWHSCH KLIYVRPNPK TGVPIGHWPV PESFWPDQNS PTLPPRTSHP VVKFSCTDCE PMV IDKLPF DKYELEPSPL TQFILERKSP QTCWQVYVSN SAKYSELGHP FGYLKASTAL NCVNLFVMPY NYPVLLPLLD DLFK VHKAK PTLKWRQSFE SYLKTMPPYY LGPLKKAVRM MGAPNLIADS MEYGLSYSVI SYLKKLSQQA KIESDRVIGS VGKKV VQET GIKVRSRSHG LSMAYRKDFQ QLLQGISEDV PHRLLDLNMK EYTGFQVALL NKDLKPQTFR NAYDIPRRNL LDHLTR MRS NLLKSTRRFL KGQDEDQVHS VPIAQMGNYQ EYLKQVPSPL RELDPDQPRR LHTFGNPFKL DKKGMMIDEA DEFVAGP QN KHKRPGEPNM QGIPKRRRCM SPLLRGRQQN PVVNNHIGGK GPPAPTTQAQ PDLIKPLPLH KISETTNDSI IHDVVENH V ADQLSSDITP NAMDTEFSAS SPASLLERPT NHMEALGHDH LGTNDLTVGG FLENHEEPRD KEQCAEENIP ASSLNKGKK LMHCRSHEEV NTELKAQIMK EIRKPGRKYE RIFTLLKHVQ GSLQTRLIFL QNVIKEASRF KKRMLIEQLE NFLDEIHRRA NQINHINSN

UniProtKB: Integrator complex subunit 6

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Macromolecule #11: Integrator complex subunit 7

MacromoleculeName: Integrator complex subunit 7 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 107.153805 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMASNSTKS FLADAGYGEQ ELDANSALME LDKGLRSGKL GEQCEAVVRF PRLFQKYPFP ILINSAFLKL ADVFRVGNNF LRLCVLKVT QQSEKHLEKI LNVDEFVKRI FSVIHSNDPV ARAITLRMLG SLASIIPERK NAHHSIRQSL DSHDNVEVEA A VFAAANFS ...String:
SNMASNSTKS FLADAGYGEQ ELDANSALME LDKGLRSGKL GEQCEAVVRF PRLFQKYPFP ILINSAFLKL ADVFRVGNNF LRLCVLKVT QQSEKHLEKI LNVDEFVKRI FSVIHSNDPV ARAITLRMLG SLASIIPERK NAHHSIRQSL DSHDNVEVEA A VFAAANFS AQSKDFAVGI CNKISEMIQG LATPVDLKLK LIPILQHMHH DAILASSARQ LLQQLVTSYP STKMVIVSLH TF TLLAASS LVDTPKQIQL LLQYLKNDPR KAVKRLAIQD LKLLANKTPH TWSRENIQAL CECALQTPYD SLKLGMLSVL STL SGTIAI KHYFSIVPGN VSSSPRSSDL VKLAQECCYH NNRGIAAHGV RVLTNITVSC QEKDLLALEQ DAVFGLESLL VLCS QDDSP GAQATLKIAL NCMVKLAKGR PHLSQSVVET LLTQLHSAQD AARILMCHCL AAIAMQLPVL GDGMLGDLME LYKVI GRSA TDKQQELLVS LATVIFVASQ KALSVESKAV IKQQLESVSN GWTVYRIARQ ASRMGNHDMA KELYQSLLTQ VASEHF YFW LNSLKEFSHA EQCLTGLQEE NYSSALSCIA ESLKFYHKGI ASLTAASTPL NPLSFQCEFV KLRIDLLQAF SQLICTC NS LKTSPPPAIA TTIAMTLGND LQRCGRISNQ MKQSMEEFRS LASRYGDLYQ ASFDADSATL RNVELQQQSC LLISHAIE A LILDPESASF QEYGSTGTAH ADSEYERRMM SVYNHVLEEV ESLNRKYTPV SYMHTACLCN AIIALLKVPL SFQRYFFQK LQSTSIKLAL SPSPRNPAEP IAVQNNQQLA LKVEGVVQHG SKPGLFRKIQ SVCLNVSSTL QSKSGQDYKI PIDNMTNEME QRVEPHNDY FSTQFLLNFA ILGTHNITVE SSVKDANGIV WKTGPRTTIF VKSLEDPYSQ QIRLQQQQAQ QPLQQQQQRN A YTRF

UniProtKB: Integrator complex subunit 7

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Macromolecule #12: Integrator complex subunit 8

MacromoleculeName: Integrator complex subunit 8 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 113.219859 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSAEAADREA ATSSRPCTPP QTCWFEFLLE ESLLEKHLRK PCPDPAPVQL IVQFLEQASK PSVNEQNQVQ PPPDNKRNRI LKLLALKVA AHLKWDLDIL EKSLSVPVLN MLLNELLCIS KVPPGTKHVD MDLATLPPTT AMAVLLYNRW AIRTIVQSSF P VKQAKPGP ...String:
MSAEAADREA ATSSRPCTPP QTCWFEFLLE ESLLEKHLRK PCPDPAPVQL IVQFLEQASK PSVNEQNQVQ PPPDNKRNRI LKLLALKVA AHLKWDLDIL EKSLSVPVLN MLLNELLCIS KVPPGTKHVD MDLATLPPTT AMAVLLYNRW AIRTIVQSSF P VKQAKPGP PQLSVMNQMQ QEKELTENIL KVLKEQAADS ILVLEAALKL NKDLYVHTMR TLDLLAMEPG MVNGETESST AG LKVKTEE MQCQVCYDLG AAYFQQGSTN SAVYENAREK FFRTKELIAE IGSLSLHCTI DEKRLAGYCQ ACDVLVPSSD STS QQLTPY SQVHICLRSG NYQEVIQIFI EDNLTLSLPV QFRQSVLREL FKKAQQGNEA LDEICFKVCA CNTVRDILEG RTIS VQFNQ LFLRPNKEKI DFLLEVCSRS VNLEKASESL KGNMAAFLKN VCLGLEDLQY VFMISSHELF ITLLKDEERK LLVDQ MRKR SPRVNLCIKP VTSFYDIPAS ASVNIGQLEH QLILSVDPWR IRQILIELHG MTSERQFWTV SNKWEVPSVY SGVILG IKD NLTRDLVYIL MAKGLHCSTV KDFSHAKQLF AACLELVTEF SPKLRQVMLN EMLLLDIHTH EAGTGQAGER PPSDLIS RV RGYLEMRLPD IPLRQVIAEE CVAFMLNWRE NEYLTLQVPA FLLQSNPYVK LGQLLAATCK ELPGPKESRR TAKDLWEV V VQICSVSSQH KRGNDGRVSL IKQRESTLGI MYRSELLSFI KKLREPLVLT IILSLFVKLH NVREDIVNDI TAEHISIWP SSIPNLQSVD FEAVAITVKE LVRYTLSINP NNHSWLIIQA DIYFATNQYS AALHYYLQAG AVCSDFFNKA VPPDVYTDQV IKRMIKCCS LLNCHTQVAI LCQFLREIDY KTAFKSLQEQ NSHDAMDSYY DYIWDVTILE YLTYLHHKRG ETDKRQIAIK A IGQTELNA SNPEEVLQLA AQRRKKKFLQ AMAKLYF

UniProtKB: Integrator complex subunit 8

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Macromolecule #13: Integrator complex subunit 9

MacromoleculeName: Integrator complex subunit 9 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 73.891219 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MKLYCLSGHP TLPCNVLKFK STTIMLDCGL DMTSTLNFLP LPLVQSPRLS NLPGWSLKDG NAFLDKELKE CSGHVFVDSV PEFCLPETE LIDLSTVDVI LISNYHCMMA LPYITEHTGF TGTVYATEPT VQIGRLLMEE LVNFIERVPK AQSASLWKNK D IQRLLPSP ...String:
MKLYCLSGHP TLPCNVLKFK STTIMLDCGL DMTSTLNFLP LPLVQSPRLS NLPGWSLKDG NAFLDKELKE CSGHVFVDSV PEFCLPETE LIDLSTVDVI LISNYHCMMA LPYITEHTGF TGTVYATEPT VQIGRLLMEE LVNFIERVPK AQSASLWKNK D IQRLLPSP LKDAVEVSTW RRCYTMQEVN SALSKIQLVG YSQKIELFGA VQVTPLSSGY ALGSSNWIIQ SHYEKVSYVS GS SLLTTHP QPMDQASLKN SDVLVLTGLT QIPTANPDGM VGEFCSNLAL TVRNGGNVLV PCYPSGVIYD LLECLYQYID SAG LSSVPL YFISPVANSS LEFSQIFAEW LCHNKQSKVY LPEPPFPHAE LIQTNKLKHY PSIHGDFSND FRQPCVVFTG HPSL RFGDV VHFMELWGKS SLNTVIFTEP DFSYLEALAP YQPLAMKCIY CPIDTRLNFI QVSKLLKEVQ PLHVVCPEQY TQPPP AQSH RMDLMIDCQP PAMSYRRAEV LALPFKRRYE KIEIMPELAD SLVPMEIKPG ISLATVSAVL HTKDNKHLLQ PPPRPA QPT SGKKRKRVSD DVPDCKVLKP LLSGSIPVEQ FVQTLEKHGF SDIKVEDTAK GHIVLLQEAE TLIQIEEDST HIICDND EM LRVRLRDLVL KFLQKF

UniProtKB: Integrator complex subunit 9

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Macromolecule #14: Integrator complex subunit 10

MacromoleculeName: Integrator complex subunit 10 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 82.339867 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSAQGDCEFL VQRARELVPQ DLWAAKAWLI TARSLYPADF NIQYEMYTIE RNAERTATAG RLLYDMFVNF PDQPVVWREI SIITSALRN DSQDKQTQFL RSLFETLPGR VQCEMLLKVT EQCFNTLERS EMLLLLLRRF PETVVQHGVG LGEALLEAET I EEQESPVN ...String:
MSAQGDCEFL VQRARELVPQ DLWAAKAWLI TARSLYPADF NIQYEMYTIE RNAERTATAG RLLYDMFVNF PDQPVVWREI SIITSALRN DSQDKQTQFL RSLFETLPGR VQCEMLLKVT EQCFNTLERS EMLLLLLRRF PETVVQHGVG LGEALLEAET I EEQESPVN CFRKLFVCDV LPLIINNHDV RLPANLLYKY LNKAAEFYIN YVTRSTQIEN QHQGAQDTSD LMSPSKRSSQ KY IIEGLTE KSSQIVDPWE RLFKILNVVG MRCEWQMDKG RRSYGDILHR MKDLCRYMNN FDSEAHAKYK NQVVYSTMLV FFK NAFQYV NSIQPSLFQG PNAPSQVPLV LLEDVSNVYG DVEIDRNKHI HKKRKLAEGR EKTMSSDDED CSAKGRNRHI VVNK AELAN STEVLESFKL ARESWELLYS LEFLDKEFTR ICLAWKTDTW LWLRIFLTDM IIYQGQYKKA IASLHHLAAL QGSIS QPQI TGQGTLEHQR ALIQLATCHF ALGEYRMTCE KVLDLMCYMV LPIQDGGKSQ EEPSKVKPKF RKGSDLKLLP CTSKAI MPY CLHLMLACFK LRAFTDNRDD MALGHVIVLL QQEWPRGENL FLKAVNKICQ QGNFQYENFF NYVTNIDMLE EFAYLRT QE GGKIHLELLP NQGMLIKHHT VTRGITKGVK EDFRLAMERQ VSRCGENLMV VLHRFCINEK ILLLQTLT

UniProtKB: Integrator complex subunit 10

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Macromolecule #15: Integrator complex subunit 11

MacromoleculeName: Integrator complex subunit 11 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 67.970781 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMPEIRVTP LGAGQDVGRS CILVSIAGKN VMLDCGMHMG FNDDRRFPDF SYITQNGRLT DFLDCVIISH FHLDHCGALP YFSEMVGYD GPIYMTHPTQ AICPILLEDY RKIAVDKKGE ANFFTSQMIK DCMKKVVAVH LHQTVQVDDE LEIKAYYAGH V LGAAMFQI ...String:
SNMPEIRVTP LGAGQDVGRS CILVSIAGKN VMLDCGMHMG FNDDRRFPDF SYITQNGRLT DFLDCVIISH FHLDHCGALP YFSEMVGYD GPIYMTHPTQ AICPILLEDY RKIAVDKKGE ANFFTSQMIK DCMKKVVAVH LHQTVQVDDE LEIKAYYAGH V LGAAMFQI KVGSESVVYT GDYNMTPDRH LGAAWIDKCR PNLLITQSTY ATTIRDSKRC RERDFLKKVH ETVERGGKVL IP VFALGRA QELCILLETF WERMNLKVPI YFSTGLTEKA NHYYKLFIPW TNQKIRKTFV QRNMFEFKHI KAFDRAFADN PGP MVVFAT PGMLHAGQSL QIFRKWAGNE KNMVIMPGYC VQGTVGHKIL SGQRKLEMEG RQVLEVKMQV EYMSFSAHAD AKGI MQLVG QAEPESVLLV HGEAKKMEFL KQKIEQELRV NCYMPANGET VTLPTSPSIP VGISLGLLKR EMAQGLLPEA KKPRL LHGT LIMKESNFRL VSSEQALKEL GLAEHQLRFT CRVHLHDTRK EQETALRVYS HLKSVLKDHC VQHLPDGSVT VESVLL QAA APSEDPGTKV LLVSWTYQDE ELGSFLTSLL KKGLPQAPS

UniProtKB: Integrator complex subunit 11

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Macromolecule #16: Integrator complex subunit 14

MacromoleculeName: Integrator complex subunit 14 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 57.526547 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MPTVVVMDVS LSMTRPVSIE GSEEYQRKHL AAHGLTMLFE HMATNYKLEF TALVVFSSLW ELMVPFTRDY NTLQEALSNM DDYDKTCLE SALVGVCNIV QQEWGGAIPC QVVLVTDGCL GIGRGSLRHS LATQNQRSES NRFPLPFPFP SKLYIMCMAN L EELQSTDS ...String:
MPTVVVMDVS LSMTRPVSIE GSEEYQRKHL AAHGLTMLFE HMATNYKLEF TALVVFSSLW ELMVPFTRDY NTLQEALSNM DDYDKTCLE SALVGVCNIV QQEWGGAIPC QVVLVTDGCL GIGRGSLRHS LATQNQRSES NRFPLPFPFP SKLYIMCMAN L EELQSTDS LECLERLIDL NNGEGQIFTI DGPLCLKNVQ SMFGKLIDLA YTPFHAVLKC GHLTADVQVF PRPEPFVVDE EI DPIPKVI NTDLEIVGFI DIADISSPPV LSRHLVLPIA LNKEGDEVGT GITDDNEDEN SANQIAGKIP NFCVLLHGSL KVE GMVAIV QLGPEWHGML YSQADSKKKS NLMMSLFEPG PEPLPWLGKM AQLGPISDAK ENPYGEDDNK SPFPLQPKNK RSYA QNVTV WIKPSGLQTD VQKILRNARK LPEKTQTFYK ELNRLRKAAL AFGFLDLLKG VADMLERECT LLPETAHPDA AFQLT HAAQ QLKLASTGTS EYAAYDQNIT PLHTDFSGSS TERI

UniProtKB: Integrator complex subunit 14

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Macromolecule #17: Integrator complex subunit 15

MacromoleculeName: Integrator complex subunit 15 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 50.303582 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMSDIRHSL LRRDALSAAK EVLYHLDIYF SSQLQSAPLP IVDKGPVELL EEFVFQVPKE RSAQPKRLNS LQELQLLEIM CNYFQEQTK DSVRQIIFSS LFSPQGNKAD DSRMSLLGKL VSMAVAVCRI PVLECAASWL QRTPVVYCVR LAKALVDDYC C LVPGSIQT ...String:
SNMSDIRHSL LRRDALSAAK EVLYHLDIYF SSQLQSAPLP IVDKGPVELL EEFVFQVPKE RSAQPKRLNS LQELQLLEIM CNYFQEQTK DSVRQIIFSS LFSPQGNKAD DSRMSLLGKL VSMAVAVCRI PVLECAASWL QRTPVVYCVR LAKALVDDYC C LVPGSIQT LKQIFSASPR FCCQFITSVT ALYDLSSDDL IPPMDLLEMI VTWIFEDPRL ILITFLNTPI AANLPIGFLE LT PLVGLIR WCVKAPLAYK RKKKPPLSNG HVSNKVTKDP GVGMDRDSHL LYSKLHLSVL QVLMTLQLHL TEKNLYGRLG LIL FDHMVP LVEEINRLAD ELNPLNASQE IELSLDRLAQ ALQVAMASGA LLCTRDDLRT LCSRLPHNNL LQLVISGPVQ QSPH AALPP GFYPHIHTPP LGYGAVPAHP AAHPALPTHP GHTFISGVTF PFRPIR

UniProtKB: Integrator complex subunit 15

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Macromolecule #18: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit...

MacromoleculeName: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 65.579531 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMAAADGDD SLYPIAVLID ELRNEDVQLR LNSIKKLSTI ALALGVERTR SELLPFLTDT IYDEDEVLLA LAEQLGTFTT LVGGPEYVH CLLPPLESLA TVEETVVRDK AVESLRAISH EHSPSDLEAH FVPLVKRLAG GDWFTSRTSA CGLFSVCYPR V SSAVKAEL ...String:
SNMAAADGDD SLYPIAVLID ELRNEDVQLR LNSIKKLSTI ALALGVERTR SELLPFLTDT IYDEDEVLLA LAEQLGTFTT LVGGPEYVH CLLPPLESLA TVEETVVRDK AVESLRAISH EHSPSDLEAH FVPLVKRLAG GDWFTSRTSA CGLFSVCYPR V SSAVKAEL RQYFRNLCSD DTPMVRRAAA SKLGEFAKVL ELDNVKSEII PMFSNLASDE QDSVRLLAVE ACVNIAQLLP QE DLEALVM PTLRQAAEDK SWRVRYMVAD KFTELQKAVG PEITKTDLVP AFQNLMKDCE AEVRAAASHK VKEFCENLSA DCR ENVIMS QILPCIKELV SDANQHVKSA LASVIMGLSP ILGKDNTIEH LLPLFLAQLK DECPEVRLNI ISNLDCVNEV IGIR QLSQS LLPAIVELAE DAKWRVRLAI IEYMPLLAGQ LGVEFFDEKL NSLCMAWLVD HVYAIREAAT SNLKKLVEKF GKEWA HATI IPKVLAMSGD PNYLHRMTTL FCINVLSEVC GQDITTKHML PTVLRMAGDP VANVRFNVAK SLQKIGPILD NSTLQS EVK PILEKLTQDQ DVDVKYFAQE ALTVLSLA

UniProtKB: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

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Macromolecule #19: Serine/threonine-protein phosphatase 2A catalytic subunit alpha i...

MacromoleculeName: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 35.836348 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMDEKVFTK ELDQWIEQLN ECKQLSESQV KSLCEKAKEI LTKESNVQEV RCPVTVCGDV HGQFHDLMEL FRIGGKSPDT NYLFMGDYV NRGYYSVETV TLLVALKVRY RERITILRGN HESRQITQVY GFYDECLRKY GNANVWKYFT DLFDYLPLTA L VDGQIFCL ...String:
SNMDEKVFTK ELDQWIEQLN ECKQLSESQV KSLCEKAKEI LTKESNVQEV RCPVTVCGDV HGQFHDLMEL FRIGGKSPDT NYLFMGDYV NRGYYSVETV TLLVALKVRY RERITILRGN HESRQITQVY GFYDECLRKY GNANVWKYFT DLFDYLPLTA L VDGQIFCL HGGLSPSIDT LDHIRALDRL QEVPHEGPMC DLLWSDPDDR GGWGISPRGA GYTFGQDISE TFNHANGLTL VS RAHQLVM EGYNWCHDRN VVTIFSAPNY CYRCGNQAAI MELDDTLKYS FLQFDPAPRR GEPHVTRRTP DYFL

UniProtKB: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

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Macromolecule #20: DSS1

MacromoleculeName: DSS1 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichoplusia ni (cabbage looper)
Molecular weightTheoretical: 3.383542 KDa
SequenceString:
DVSVWEENWE DDIVQDDFNQ QLRLEME

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Macromolecule #21: Integrator complex subunit 13

MacromoleculeName: Integrator complex subunit 13 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 80.34518 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MKIFSESHKT VFVVDHCPYM AESCRQHVEF DMLVKNRTQG IIPLAPISKS LWTCSVESSM EYCRIMYDIF PFKKLVNFIV SDSGAHVLN SWTQEDQNLQ ELMAALAAVG PPNPRADPEC CSILHGLVAA VETLCKITEY QHEARTLLME NAERVGNRGR I ICITNAKS ...String:
MKIFSESHKT VFVVDHCPYM AESCRQHVEF DMLVKNRTQG IIPLAPISKS LWTCSVESSM EYCRIMYDIF PFKKLVNFIV SDSGAHVLN SWTQEDQNLQ ELMAALAAVG PPNPRADPEC CSILHGLVAA VETLCKITEY QHEARTLLME NAERVGNRGR I ICITNAKS DSHVRMLEDC VQETIHEHNK LAANSDHLMQ IQKCELVLIH TYPVGEDSLV SDRSKKELSP VLTSEVHSVR AG RHLATKL NILVQQHFDL ASTTITNIPM KEEQHANTSA NYDVELLHHK DAHVDFLKSG DSHLGGGSRE GSFKETITLK WCT PRTNNI ELHYCTGAYR ISPVDVNSRP SSCLTNFLLN GRSVLLEQPR KSGSKVISHM LSSHGGEIFL HVLSSSRSIL EDPP SISEG CGGRVTDYRI TDFGEFMREN RLTPFLDPRY KIDGSLEVPL ERAKDQLEKH TRYWPMIISQ TTIFNMQAVV PLASV IVKE SLTEEDVLNC QKTIYNLVDM ERKNDPLPIS TVGTRGKGPK RDEQYRIMWN ELETLVRAHI NNSEKHQRVL ECLMAC RSK PPEEEERKKR GRKREDKEDK SEKAVKDYEQ EKSWQDSERL KGILERGKEE LAEAEIIKDS PDSPEPPNKK PLVEMDE TP QVEKSKGPVS LLSLWSNRIN TANSRKHQEF AGRLNSVNNR AELYQHLKEE NGMETTENGK ASRQ

UniProtKB: Integrator complex subunit 13

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Macromolecule #22: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 22 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #23: MANGANESE (II) ION

MacromoleculeName: MANGANESE (II) ION / type: ligand / ID: 23 / Number of copies: 2 / Formula: MN
Molecular weightTheoretical: 54.938 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.3 mg/mL
BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 39.93 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: INSILICO MODEL
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: NOT APPLICABLE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 236382
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

4oww


Chain - Source name: PDB / Chain - Initial model type: experimental model
Output model

PDB-8rbz:
Structure of Integrator-PP2A-SOSS-CTD post-termination complex

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Atomic model buiding 2

Initial modelPDB ID:

8rbx


Chain - Source name: PDB / Chain - Initial model type: experimental model
Output model

PDB-8rbz:
Structure of Integrator-PP2A-SOSS-CTD post-termination complex

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