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- EMDB-19038: Structure of Integrator-PP2A bound to a paused RNA polymerase II-... -

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Basic information

Entry
Database: EMDB / ID: EMD-19038
TitleStructure of Integrator-PP2A bound to a paused RNA polymerase II-DSIF-NELF-nucleosome complex
Map dataOverall map filtered by local resolution
Sample
  • Complex: Pre-termination complex
    • Complex: Integrator-PP2A complex and NELF
      • Protein or peptide: x 19 types
    • Complex: Nucleosome
      • Protein or peptide: x 5 types
    • Complex: RNA and DNA
      • DNA: x 2 types
      • RNA: x 1 types
    • Complex: DNA-directed RNA Polymerase IIPolymerase
      • Protein or peptide: x 13 types
    • Complex: DSIF
      • Protein or peptide: x 1 types
  • Ligand: x 3 types
KeywordsIntegrator complex / Pre-termination complex / TRANSCRIPTION
Function / homology
Function and homology information


regulation of fertilization / U2 snRNA 3'-end processing / NELF complex / positive regulation of protein modification process / snRNA processing / integrator complex / snRNA 3'-end processing / meiotic spindle elongation / Integration of energy metabolism / PP2A-mediated dephosphorylation of key metabolic factors ...regulation of fertilization / U2 snRNA 3'-end processing / NELF complex / positive regulation of protein modification process / snRNA processing / integrator complex / snRNA 3'-end processing / meiotic spindle elongation / Integration of energy metabolism / PP2A-mediated dephosphorylation of key metabolic factors / NTRK3 as a dependence receptor / regulation of microtubule binding / negative regulation of DNA-templated transcription, elongation / MASTL Facilitates Mitotic Progression / mitotic sister chromatid separation / regulation of meiotic cell cycle process involved in oocyte maturation / protein phosphatase type 2A complex / flagellated sperm motility / meiotic sister chromatid cohesion, centromeric / peptidyl-serine dephosphorylation / DSIF complex / protein localization to nuclear envelope / regulation of transcription elongation by RNA polymerase II / peptidyl-threonine dephosphorylation / : / positive regulation of microtubule binding / negative regulation of tyrosine phosphorylation of STAT protein / Regulation of glycolysis by fructose 2,6-bisphosphate metabolism / Inhibition of replication initiation of damaged DNA by RB1/E2F1 / female meiotic nuclear division / protein antigen binding / protein phosphatase regulator activity / ceramide metabolic process / GABA receptor binding / negative regulation of epithelial to mesenchymal transition / APC truncation mutants have impaired AXIN binding / AXIN missense mutants destabilize the destruction complex / Truncations of AMER1 destabilize the destruction complex / B-WICH complex positively regulates rRNA expression / RNA Polymerase I Transcription Initiation / RNA Polymerase I Promoter Escape / RNA Polymerase I Transcription Termination / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / Initiation of Nuclear Envelope (NE) Reformation / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Major Pathway / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / ERKs are inactivated / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / negative regulation of stem cell differentiation / nuclear lumen / positive regulation of extrinsic apoptotic signaling pathway in absence of ligand / Beta-catenin phosphorylation cascade / Signaling by GSK3beta mutants / CTNNB1 S33 mutants aren't phosphorylated / CTNNB1 S37 mutants aren't phosphorylated / CTNNB1 S45 mutants aren't phosphorylated / CTNNB1 T41 mutants aren't phosphorylated / Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters / Abortive elongation of HIV-1 transcript in the absence of Tat / regulation of Wnt signaling pathway / positive regulation of DNA-templated transcription, elongation / Disassembly of the destruction complex and recruitment of AXIN to the membrane / regulation of growth / nucleosomal DNA binding / centrosome localization / transcription elongation-coupled chromatin remodeling / : / inner cell mass cell proliferation / negative regulation of glycolytic process through fructose-6-phosphate / positive regulation of NLRP3 inflammasome complex assembly / myosin phosphatase activity / protein serine/threonine phosphatase activity / CTLA4 inhibitory signaling / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / Platelet sensitization by LDL / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / mRNA Capping / negative regulation of MAPK cascade
Similarity search - Function
Integrator complex subunit 10 / Integrator complex subunit 15 / Integrator complex subunit 15 / Integrator subunit 10 / Cell cycle regulator Mat89Bb / Integrator complex subunit 14 / Integrator complex subunit 14, beta-barrel domain / Integrator complex subunit 14, C-terminal / Cell cycle and development regulator Mat89Bb / Integrator complex subunit 14, beta-barrel domain ...Integrator complex subunit 10 / Integrator complex subunit 15 / Integrator complex subunit 15 / Integrator subunit 10 / Cell cycle regulator Mat89Bb / Integrator complex subunit 14 / Integrator complex subunit 14, beta-barrel domain / Integrator complex subunit 14, C-terminal / Cell cycle and development regulator Mat89Bb / Integrator complex subunit 14, beta-barrel domain / Integrator complex subunit 14, alpha-helical domain / Domain of unknown function DUF3677 / Integrator complex subunit 2, metazoa / Integrator complex subunit 2 / Integrator complex subunit 5, C-terminal / Integrator complex subunit 5, N-terminal / Integrator complex subunit 7 / Integrator complex subunit 8 / Integrator complex subunit 1 / Integrator complex subunit 5 / Protein of unknown function (DUF3677) / Integrator complex subunit 2 / Integrator complex subunit 5 N-terminus / Integrator complex subunit 5 C-terminus / INTS6/SAGE1/DDX26B/CT45, C-terminal / INTS6/SAGE1/DDX26B/CT45 C-terminus / Cofactor of BRCA1 / Cofactor of BRCA1 (COBRA1) / : / : / Integrator IntS9, C-terminal domain / Integrator IntS11, C-terminal domain / TH1 protein / Integrator complex subunit 9 / Integrator complex subunit 11, MBL-fold / TH1 protein / Sister chromatid cohesion protein PDS5 protein / Hepatitis delta antigen (HDAg) domain / Hepatitis delta antigen (HDAg) domain profile. / Metallo-beta-lactamase superfamily domain / : / Beta-Casp domain / Beta-Casp domain / Beta-Casp domain / Spt5, KOW domain repeat 6 / Zn-dependent metallo-hydrolase, RNA specificity domain / Zn-dependent metallo-hydrolase RNA specificity domain / HEAT repeat / Spt5 C-terminal domain / HEAT repeat / Spt5 C-terminal nonapeptide repeat binding Spt4 / Transcription elongation factor Spt5, eukaryote / Spt5 transcription elongation factor, N-terminal / Spt5, KOW domain repeat 2 / Spt5, KOW domain repeat 3 / Spt5, KOW domain repeat 5 / Spt5 transcription elongation factor, acidic N-terminal / NGN domain, eukaryotic / Spt5, KOW domain repeat 1 / Spt5, KOW domain repeat 4 / NGN domain / Transcription elongation factor SPT5 / Early transcription elongation factor of RNA pol II, NGN section / Serine/threonine specific protein phosphatases signature. / Protein phosphatase 2A homologues, catalytic domain. / Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase / HEAT repeat profile. / HEAT, type 2 / von Willebrand factor type A domain / HEAT repeats / NusG, N-terminal / In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold. / NusG, N-terminal domain superfamily / Calcineurin-like phosphoesterase domain, ApaH type / Calcineurin-like phosphoesterase / DNA-directed RNA polymerase II subunit Rpb4-like / RNA polymerase Rpb4/RPC9, core / DNA-directed RNA-polymerase II subunit / RNA polymerase II, heptapeptide repeat, eukaryotic / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1 C-terminal repeat / Eukaryotic RNA polymerase II heptapeptide repeat. / Metallo-dependent phosphatase-like / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / Rpb4/RPC9 superfamily / Pol II subunit B9, C-terminal zinc ribbon / RNA polymerase RBP11 / RNA polymerase subunit Rpb4/RPC9 / RNA polymerase Rpb4 / Zinc finger TFIIS-type signature. / RNA polymerase subunit Rpb7-like / HRDC-like superfamily / RNA polymerase Rpb7-like , N-terminal / RNA polymerase Rpb7-like, N-terminal domain superfamily / SHS2 domain found in N terminus of Rpb7p/Rpc25p/MJ0397 / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 4
Similarity search - Domain/homology
RNA polymerase II subunit D / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase subunit / RNA polymerase II subunit K / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase II subunit RPB11-a / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase subunit beta ...RNA polymerase II subunit D / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase subunit / RNA polymerase II subunit K / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase II subunit RPB11-a / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase subunit beta / DNA-directed RNA polymerase II subunit E / Transcription elongation factor SPT5 / Histone H2A type 1 / Histone H2B type 1-J / Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform / DNA-directed RNA polymerase II subunit RPB9 / Histone H4 / Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform / Integrator complex subunit 11 / Histone H3.3C / Integrator complex subunit 5 / Integrator complex subunit 8 / Negative elongation factor C/D / Integrator complex subunit 1 / Negative elongation factor B / Integrator complex subunit 4 / Integrator complex subunit 15 / Integrator complex subunit 14 / Integrator complex subunit 2 / Negative elongation factor A / Integrator complex subunit 9 / Integrator complex subunit 7 / Integrator complex subunit 13 / Integrator complex subunit 10 / Integrator complex subunit 6
Similarity search - Component
Biological speciesHomo sapiens (human) / Sus scrofa (pig) / Trichoplusia ni (cabbage looper) / synthetic construct (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.1 Å
AuthorsFianu I / Ochmann M / Walshe JL / Cramer P
Funding support Germany, European Union, 3 items
OrganizationGrant numberCountry
German Research Foundation (DFG)SFB860 Germany
German Research Foundation (DFG)SFB1565 Germany
European Research Council (ERC)EXC 2067/1-390729940European Union
CitationJournal: Nature / Year: 2024
Title: Structural basis of Integrator-dependent RNA polymerase II termination.
Authors: Isaac Fianu / Moritz Ochmann / James L Walshe / Olexandr Dybkov / Joseph Neos Cruz / Henning Urlaub / Patrick Cramer /
Abstract: The Integrator complex can terminate RNA polymerase II (Pol II) in the promoter-proximal region of genes. Previous work has shed light on how Integrator binds to the paused elongation complex ...The Integrator complex can terminate RNA polymerase II (Pol II) in the promoter-proximal region of genes. Previous work has shed light on how Integrator binds to the paused elongation complex consisting of Pol II, the DRB sensitivity-inducing factor (DSIF) and the negative elongation factor (NELF) and how it cleaves the nascent RNA transcript, but has not explained how Integrator removes Pol II from the DNA template. Here we present three cryo-electron microscopy structures of the complete Integrator-PP2A complex in different functional states. The structure of the pre-termination complex reveals a previously unresolved, scorpion-tail-shaped INTS10-INTS13-INTS14-INTS15 module that may use its 'sting' to open the DSIF DNA clamp and facilitate termination. The structure of the post-termination complex shows that the previously unresolved subunit INTS3 and associated sensor of single-stranded DNA complex (SOSS) factors prevent Pol II rebinding to Integrator after termination. The structure of the free Integrator-PP2A complex in an inactive closed conformation reveals that INTS6 blocks the PP2A phosphatase active site. These results lead to a model for how Integrator terminates Pol II transcription in three steps that involve major rearrangements.
History
DepositionDec 5, 2023-
Header (metadata) releaseFeb 7, 2024-
Map releaseFeb 7, 2024-
UpdateApr 10, 2024-
Current statusApr 10, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_19038.png

Downloads & links

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Map

FileDownload / File: emd_19038.map.gz / Format: CCP4 / Size: 476.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationOverall map filtered by local resolution
Voxel sizeX=Y=Z: 1.05 Å
Density
Contour LevelBy AUTHOR: 0.00453
Minimum - Maximum-0.045693368 - 0.10492225
Average (Standard dev.)-0.000028854836 (±0.0024531495)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions500500500
Spacing500500500
CellA=B=C: 525.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_19038_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Focused refined map around Pol II, DSIF, NELF and nucleic acids

Fileemd_19038_additional_1.map
AnnotationFocused refined map around Pol II, DSIF, NELF and nucleic acids
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Additional map: Focused refined map around INTS5, INTS6, INTS8 and PP2A

Fileemd_19038_additional_2.map
AnnotationFocused refined map around INTS5, INTS6, INTS8 and PP2A
Projections & Slices
AxesZYX

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Additional map: Focused refined map around PP2A

Fileemd_19038_additional_3.map
AnnotationFocused refined map around PP2A
Projections & Slices
AxesZYX

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Additional map: Focused refined map around Integrator cleavage module (INTS4,...

Fileemd_19038_additional_4.map
AnnotationFocused refined map around Integrator cleavage module (INTS4, INTS9 and INTS11)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Additional map: Focused refined map around INTS1, INTS2 INTS7 and DSS1

Fileemd_19038_additional_5.map
AnnotationFocused refined map around INTS1, INTS2 INTS7 and DSS1
Projections & Slices
AxesZYX

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Additional map: Focused refined map around Integrator tail module (INTS10,...

Fileemd_19038_additional_6.map
AnnotationFocused refined map around Integrator tail module (INTS10, INTS13 , INTS14 and INTS15)
Projections & Slices
AxesZYX

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Histogram

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Half map: #2

Fileemd_19038_half_map_1.map
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Half map: #1

Fileemd_19038_half_map_2.map
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Sample components

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Entire : Pre-termination complex

EntireName: Pre-termination complex
Components
  • Complex: Pre-termination complex
    • Complex: Integrator-PP2A complex and NELF
      • Protein or peptide: Integrator complex subunit 1
      • Protein or peptide: Integrator complex subunit 2
      • Protein or peptide: Integrator complex subunit 4
      • Protein or peptide: Integrator complex subunit 5
      • Protein or peptide: Integrator complex subunit 6
      • Protein or peptide: Integrator complex subunit 7
      • Protein or peptide: Integrator complex subunit 8
      • Protein or peptide: Integrator complex subunit 9
      • Protein or peptide: Integrator complex subunit 10
      • Protein or peptide: Integrator complex subunit 11
      • Protein or peptide: Integrator complex subunit 13
      • Protein or peptide: Integrator complex subunit 14
      • Protein or peptide: Integrator complex subunit 15
      • Protein or peptide: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
      • Protein or peptide: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
      • Protein or peptide: Negative elongation factor A
      • Protein or peptide: NELF-B,Negative elongation factor B,Negative elongation factor B,Negative elongation factor B,Negative elongation factor B,Negative elongation factor B
      • Protein or peptide: Negative elongation factor C/D
      • Protein or peptide: UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK
    • Complex: Nucleosome
      • Protein or peptide: Histone H3.3C
      • Protein or peptide: Histone H4
      • Protein or peptide: Histone H2A type 1
      • Protein or peptide: Histone H2B type 1-J
      • Protein or peptide: Transcription elongation factor SPT5
    • Complex: RNA and DNA
      • DNA: non-template DNA
      • RNA: RNA
      • DNA: Template DNA
    • Complex: DNA-directed RNA Polymerase IIPolymerase
      • Protein or peptide: UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK
      • Protein or peptide: DNA-directed RNA polymerase subunitPolymerase
      • Protein or peptide: DNA-directed RNA polymerase subunit betaPolymerase
      • Protein or peptide: DNA-directed RNA polymerase II subunit RPB3Polymerase
      • Protein or peptide: RNA polymerase II subunit D
      • Protein or peptide: DNA-directed RNA polymerase II subunit EPolymerase
      • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC2RNA polymerase
      • Protein or peptide: DNA-directed RNA polymerase II subunit RPB7Polymerase
      • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC3RNA polymerase
      • Protein or peptide: DNA-directed RNA polymerase II subunit RPB9Polymerase
      • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC5RNA polymerase
      • Protein or peptide: DNA-directed RNA polymerase II subunit RPB11-aPolymerase
      • Protein or peptide: RNA polymerase II subunit K
    • Complex: DSIF
      • Protein or peptide: DSS1
  • Ligand: ZINC ION
  • Ligand: MAGNESIUM ION
  • Ligand: MANGANESE (II) ION

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Supramolecule #1: Pre-termination complex

SupramoleculeName: Pre-termination complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#41
Details: Integrator-PP2A, RNA Polymerase II, NELF, DSIF, nucleosome
Molecular weightTheoretical: 1.8 MDa

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Supramolecule #2: Integrator-PP2A complex and NELF

SupramoleculeName: Integrator-PP2A complex and NELF / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #22-#36, #38-#41
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #3: Nucleosome

SupramoleculeName: Nucleosome / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #14, #16, #18-#19, #21
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #5: RNA and DNA

SupramoleculeName: RNA and DNA / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #15, #17, #20
Source (natural)Organism: Sus scrofa (pig)

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Supramolecule #4: DNA-directed RNA Polymerase II

SupramoleculeName: DNA-directed RNA Polymerase II / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #1-#13
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #6: DSIF

SupramoleculeName: DSIF / type: complex / ID: 6 / Parent: 1 / Macromolecule list: #37
Source (natural)Organism: Trichoplusia ni (cabbage looper)

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Macromolecule #1: UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK

MacromoleculeName: UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 698.854 Da
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #2: DNA-directed RNA polymerase subunit

MacromoleculeName: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 217.450078 KDa
SequenceString: MHGGGPPSGD SACPLRTIKR VQFGVLSPDE LKRMSVTEGG IKYPETTEGG RPKLGGLMDP RQGVIERTGR CQTCAGNMTE CPGHFGHIE LAKPVFHVGF LVKTMKVLRC VCFFCSKLLV DSNNPKIKDI LAKSKGQPKK RLTHVYDLCK GKNICEGGEE M DNKFGVEQ ...String:
MHGGGPPSGD SACPLRTIKR VQFGVLSPDE LKRMSVTEGG IKYPETTEGG RPKLGGLMDP RQGVIERTGR CQTCAGNMTE CPGHFGHIE LAKPVFHVGF LVKTMKVLRC VCFFCSKLLV DSNNPKIKDI LAKSKGQPKK RLTHVYDLCK GKNICEGGEE M DNKFGVEQ PEGDEDLTKE KGHGGCGRYQ PRIRRSGLEL YAEWKHVNED SQEKKILLSP ERVHEIFKRI SDEECFVLGM EP RYARPEW MIVTVLPVPP LSVRPAVVMQ GSARNQDDLT HKLADIVKIN NQLRRNEQNG AAAHVIAEDV KLLQFHVATM VDN ELPGLP RAMQKSGRPL KSLKQRLKGK EGRVRGNLMG KRVDFSARTV ITPDPNLSID QVGVPRSIAA NMTFAEIVTP FNID RLQEL VRRGNSQYPG AKYIIRDNGD RIDLRFHPKP SDLHLQTGYK VERHMCDGDI VIFNRQPTLH KMSMMGHRVR ILPWS TFRL NLSVTTPYNA DFDGDEMNLH LPQSLETRAE IQELAMVPRM IVTPQSNRPV MGIVQDTLTA VRKFTKRDVF LERGEV MNL LMFLSTWDGK VPQPAILKPR PLWTGKQIFS LIIPGHINCI RTHSTHPDDE DSGPYKHISP GDTKVVVENG ELIMGIL CK KSLGTSAGSL VHISYLEMGH DITRLFYSNI QTVINNWLLI EGHTIGIGDS IADSKTYQDI QNTIKKAKQD VIEVIEKA H NNELEPTPGN TLRQTFENQV NRILNDARDK TGSSAQKSLS EYNNFKSMVV SGAKGSKINI SQVIAVVGQQ NVEGKRIPF GFKHRTLPHF IKDDYGPESR GFVENSYLAG LTPTEFFFHA MGGREGLIDT AVKTAETGYI QRRLIKSMES VMVKYDATVR NSINQVVQL RYGEDGLAGE SVEFQNLATL KPSNKAFEKK FRFDYTNERA LRRTLQEDLV KDVLSNAHIQ NELEREFERM R EDREVLRV IFPTGDSKVV LPCNLLRMIW NAQKIFHINP RLPSDLHPIK VVEGVKELSK KLVIVNGDDP LSRQAQENAT LL FNIHLRS TLCSRRMAEE FRLSGEAFDW LLGEIESKFN QAIAHPGEMV GALAAQSLGE PATQMTLNTF HYAGVSAKNV TLG VPRLKE LINISKKPKT PSLTVFLLGQ SARDAERAKD ILCRLEHTTL RKVTANTAIY YDPNPQSTVV AEDQEWVNVY YEMP DFDVA RISPWLLRVE LDRKHMTDRK LTMEQIAEKI NAGFGDDLNC IFNDDNAEKL VLRIRIMNSD ENKMQEEEEV VDKMD DDVF LRCIESNMLT DMTLQGIEQI SKVYMHLPQT DNKKKIIITE DGEFKALQEW ILETDGVSLM RVLSEKDVDP VRTTSN DIV EIFTVLGIEA VRKALERELY HVISFDGSYV NYRHLALLCD TMTCRGHLMA ITRHGVNRQD TGPLMKCSFE ETVDVLM EA AAHGESDPMK GVSENIMLGQ LAPAGTGCFD LLLDAEKCKY GMEIPTNIPG LGAAGPTGMF FGSAPSPMGG ISPAMTPW N QGATPAYGAW SPSVGSGMTP GAAGFSPSAA SDASGFSPGY SPAWSPTPGS PGSPGPSSPY IPSPGGAMSP SYSPTSPAY EPRSPGGYTP QSPSYSPTSP SYSPTSPSYS PTSPNYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPTSPNYS PTSPNYTPTS P SYSPTSPS YSPTSPNYTP TSPNYSPTSP SYSPTSPSYS PTSPSYSPSS PRYTPQSPTY TPSSPSYSPS SPSYSPTSPK YT PTSPSYS PSSPEYTPTS PKYSPTSPKY SPTSPKYSPT SPTYSPTTPK YSPTSPTYSP TSPVYTPTSP KYSPTSPTYS PTS PKYSPT SPTYSPTSPK GSTYSPTSPG YSPTSPTYSL TSPAISPDDS DEEN

UniProtKB: DNA-directed RNA polymerase subunit

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Macromolecule #3: DNA-directed RNA polymerase subunit beta

MacromoleculeName: DNA-directed RNA polymerase subunit beta / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 134.041422 KDa
SequenceString: MYDADEDMQY DEDDDEITPD LWQEACWIVI SSYFDEKGLV RQQLDSFDEF IQMSVQRIVE DAPPIDLQAE AQHASGEVEE PPRYLLKFE QIYLSKPTHW ERDGAPSPMM PNEARLRNLT YSAPLYVDIT KTVIKEGEEQ LQTQHQKTFI GKIPIMLRST Y CLLNGLTD ...String:
MYDADEDMQY DEDDDEITPD LWQEACWIVI SSYFDEKGLV RQQLDSFDEF IQMSVQRIVE DAPPIDLQAE AQHASGEVEE PPRYLLKFE QIYLSKPTHW ERDGAPSPMM PNEARLRNLT YSAPLYVDIT KTVIKEGEEQ LQTQHQKTFI GKIPIMLRST Y CLLNGLTD RDLCELNECP LDPGGYFIIN GSEKVLIAQE KMATNTVYVF AKKDSKYAYT GECRSCLENS SRPTSTIWVS ML ARGGQGA KKSAIGQRIV ATLPYIKQEV PIIIVFRALG FVSDRDILEH IIYDFEDPEM MEMVKPSLDE AFVIQEQNVA LNF IGSRGA KPGVTKEKRI KYAKEVLQKE MLPHVGVSDF CETKKAYFLG YMVHRLLLAA LGRRELDDRD HYGNKRLDLA GPLL AFLFR GMFKNLLKEV RIYAQKFIDR GKDFNLELAI KTRIISDGLK YSLATGNWGD QKKAHQARAG VSQVLNRLTF ASTLS HLRR LNSPIGRDGK LAKPRQLHNT LWGMVCPAET PEGHAVGLVK NLALMAYISV GSQPSPILEF LEEWSMENLE EISPAA IAD ATKIFVNGCW VGIHKDPEQL MNTLRKLRRQ MDIIVSEVSM IRDIREREIR IYTDAGRICR PLLIVEKQKL LLKKRHI DQ LKEREYNNYS WQDLVASGVV EYIDTLEEET VMLAMTPDDL QEKEVAYCST YTHCEIHPSM ILGVCASIIP FPDHNQSP R NTYQSAMGKQ AMGVYITNFH VRMDTLAHVL YYPQKPLVTT RSMEYLRFRE LPAGINSIVA IASYTGYNQE DSVIMNRSA VDRGFFRSVF YRSYKEQESK KGFDQEEVFE KPTRETCQGM RHAIYDKLDD DGLIAPGVRV SGDDVIIGKT VTLPENEDEL EGTNRRYTK RDCSTFLRTS ETGIVDQVMV TLNQEGYKFC KIRVRSVRIP QIGDKFASRH GQKGTCGIQY RQEDMPFTCE G ITPDIIIN PHAIPSRMTI GHLIECLQGK VSANKGEIGD ATPFNDAVNV QKISNLLSDY GYHLRGNEVL YNGFTGRKIT SQ IFIGPTY YQRLKHMVDD KIHSRARGPI QILNRQPMEG RSRDGGLRFG EMERDCQIAH GAAQFLRERL FEASDPYQVH VCN LCGIMA IANTRTHTYE CRGCRNKTQI SLVRMPYACK LLFQELMSMS IAPRMMSV

UniProtKB: DNA-directed RNA polymerase subunit beta

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Macromolecule #4: DNA-directed RNA polymerase II subunit RPB3

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 31.439074 KDa
SequenceString: MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV QIDANSSVLH DEFIAHRLGL IPLTSDDIVD KLQYSRDCT CEEFCPECSV EFTLDVRCNE DQTRHVTSRD LISNSPRVIP VTSRNRDNDP SDYVEQDDIL IVKLRKGQEL R LRAYAKKG ...String:
MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV QIDANSSVLH DEFIAHRLGL IPLTSDDIVD KLQYSRDCT CEEFCPECSV EFTLDVRCNE DQTRHVTSRD LISNSPRVIP VTSRNRDNDP SDYVEQDDIL IVKLRKGQEL R LRAYAKKG FGKEHAKWNP TAGVAFEYDP DNALRHTVYP KPEEWPKSEY SELDEDESQA PYDPNGKPER FYYNVESCGS LR PETIVLS ALSGLKKKLS DLQTQLSHEI QSDVLTIN

UniProtKB: DNA-directed RNA polymerase II subunit RPB3

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Macromolecule #5: RNA polymerase II subunit D

MacromoleculeName: RNA polymerase II subunit D / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 16.331255 KDa
SequenceString:
MAAGGSDPRA GDVEEDASQL IFPKEFETAE TLLNSEVHML LEHRKQQNES AEDEQELSEV FMKTLNYTAR FSRFKNRETI ASVRSLLLQ KKLHKFELAC LANLCPETAE ESKALIPSLE GRFEDEELQQ ILDDIQTKRS FQY

UniProtKB: RNA polymerase II subunit D

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Macromolecule #6: DNA-directed RNA polymerase II subunit E

MacromoleculeName: DNA-directed RNA polymerase II subunit E / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 24.644318 KDa
SequenceString: MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQFGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKV YCQRMQEENI TRALIVVQQG MTPSAKQSLV DMAPKYILEQ FLQQELLINI TEHELVPEHV VMTKEEVTEL L ARYKLREN ...String:
MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQFGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKV YCQRMQEENI TRALIVVQQG MTPSAKQSLV DMAPKYILEQ FLQQELLINI TEHELVPEHV VMTKEEVTEL L ARYKLREN QLPRIQAGDP VARYFGIKRG QVVKIIRPSE TAGRYITYRL VQ

UniProtKB: DNA-directed RNA polymerase II subunit E

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Macromolecule #7: DNA-directed RNA polymerases I, II, and III subunit RPABC2

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC2
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 14.477001 KDa
SequenceString:
MSDNEDNFDG DDFDDVEEDE GLDDLENAEE EGQENVEILP SGERPQANQK RITTPYMTKY ERARVLGTRA LQIAMCAPVM VELEGETDP LLIAMKELKA RKIPIIIRRY LPDGSYEDWG VDELIISD

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC2

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Macromolecule #8: DNA-directed RNA polymerase II subunit RPB7

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB7 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 19.314283 KDa
SequenceString:
MFYHISLEHE ILLHPRYFGP NLLNTVKQKL FTEVEGTCTG KYGFVIAVTT IDNIGAGVIQ PGRGFVLYPV KYKAIVFRPF KGEVVDAVV TQVNKVGLFT EIGPMSCFIS RHSIPSEMEF DPNSNPPCYK TMDEDIVIQQ DDEIRLKIVG TRVDKNDIFA I GSLMDDYL GLVS

UniProtKB: DNA-directed RNA polymerase subunit

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Macromolecule #9: DNA-directed RNA polymerases I, II, and III subunit RPABC3

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC3
type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 17.162273 KDa
SequenceString:
MAGILFEDIF DVKDIDPEGK KFDRVSRLHC ESESFKMDLI LDVNIQIYPV DLGDKFRLVI ASTLYEDGTL DDGEYNPTDD RPSRADQFE YVMYGKVYRI EGDETSTEAA TRLSAYVSYG GLLMRLQGDA NNLHGFEVDS RVYLLMKKLA F

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC3

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Macromolecule #10: DNA-directed RNA polymerase II subunit RPB9

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB9 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 14.541221 KDa
SequenceString:
MEPDGTYEPG FVGIRFCQEC NNMLYPKEDK ENRILLYACR NCDYQQEADN SCIYVNKITH EVDELTQIIA DVSQDPTLPR TEDHPCQKC GHKEAVFFQS HSARAEDAMR LYYVCTAPHC GHRWTE

UniProtKB: DNA-directed RNA polymerase II subunit RPB9

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Macromolecule #11: DNA-directed RNA polymerases I, II, and III subunit RPABC5

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC5
type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 7.655123 KDa
SequenceString:
MIIPVRCFTC GKIVGNKWEA YLGLLQAEYT EGDALDALGL KRYCCRRMLL AHVDLIEKLL NYAPLEK

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC5

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Macromolecule #12: DNA-directed RNA polymerase II subunit RPB11-a

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB11-a / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 13.310284 KDa
SequenceString:
MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTN AITDLISELS LLEERFRVAI KDKQEGIE

UniProtKB: DNA-directed RNA polymerase II subunit RPB11-a

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Macromolecule #13: RNA polymerase II subunit K

MacromoleculeName: RNA polymerase II subunit K / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 7.018244 KDa
SequenceString:
MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK RLVVFDAR

UniProtKB: RNA polymerase II subunit K

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Macromolecule #14: Histone H3.3C

MacromoleculeName: Histone H3.3C / type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.350021 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MARTKQTARK STGGKAPRKQ LATKAARKST PSTCGVKPHR YRPGTVALRE IRRYQKSTEL LIRKLPFQRL VREIAQDFKT DLRFQSSAV MALQEASEAY LVGLLEDTNL CAIHAKRVTI MPKDIQLARR IRGERA

UniProtKB: Histone H3.3C

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Macromolecule #16: Histone H4

MacromoleculeName: Histone H4 / type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 11.394426 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MSGRGKGGKG LGKGGAKRHR KVLRDNIQGI TKPAIRRLAR RGGVKRISGL IYEETRGVLK VFLENVIRDA VTYTEHAKRK TVTAMDVVY ALKRQGRTLY GFGG

UniProtKB: Histone H4

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Macromolecule #18: Histone H2A type 1

MacromoleculeName: Histone H2A type 1 / type: protein_or_peptide / ID: 18
Details: MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAVRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKKTESHHKAKGK
Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.093484 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MSGRGKQGGK ARAKAKSRSS RAGLQFPVGR VHRLLRKGNY AERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAVR NDEELNKLLG KVTIAQGGVL PNIQAVLLPK KTESHHKAKG K

UniProtKB: Histone H2A type 1

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Macromolecule #19: Histone H2B type 1-J

MacromoleculeName: Histone H2B type 1-J / type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.981267 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MPEPSKSAPA PKKGSKKAIT KAQKKDGKKR KRSRKESYSI YVYKVLKQVH PDTGISSKAM GIMNSFVNDI FERIAGEASR LAHYNKRST ITSREIQTAV RLLLPGELAK HAVSEGTKAV TKYTSSK

UniProtKB: Histone H2B type 1-J

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Macromolecule #21: Transcription elongation factor SPT5

MacromoleculeName: Transcription elongation factor SPT5 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 121.145477 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MSDSEDSNFS EEEDSERSSD GEEAEVDEER RSAAGSEKEE EPEDEEEEEE EEEYDEEEEE EDDDRPPKKP RHGGFILDEA DVDDEYEDE DQWEDGAEDI LEKEEIEASN IDNVVLDEDR SGARRLQNLW RDQREEELGE YYMKKYAKSS VGETVYGGSD E LSDDITQQ ...String:
MSDSEDSNFS EEEDSERSSD GEEAEVDEER RSAAGSEKEE EPEDEEEEEE EEEYDEEEEE EDDDRPPKKP RHGGFILDEA DVDDEYEDE DQWEDGAEDI LEKEEIEASN IDNVVLDEDR SGARRLQNLW RDQREEELGE YYMKKYAKSS VGETVYGGSD E LSDDITQQ QLLPGVKDPN LWTVKCKIGE ERATAISLMR KFIAYQFTDT PLQIKSVVAP EHVKGYIYVE AYKQTHVKQA IE GVGNLRL GYWNQQMVPI KEMTDVLKVV KEVANLKPKS WVRLKRGIYK DDIAQVDYVE PSQNTISLKM IPRIDYDRIK ARM SLKDWF AKRKKFKRPP QRLFDAEKIR SLGGDVASDG DFLIFEGNRY SRKGFLFKSF AMSAVITEGV KPTLSELEKF EDQP EGIDL EVVTESTGKE REHNFQPGDN VEVCEGELIN LQGKILSVDG NKITIMPKHE DLKDMLEFPA QELRKYFKMG DHVKV IAGR FEGDTGLIVR VEENFVILFS DLTMHELKVL PRDLQLCSET ASGVDVGGQH EWGELVQLDP QTVGVIVRLE RETFQV LNM YGKVVTVRHQ AVTRKKDNRF AVALDSEQNN IHVKDIVKVI DGPHSGREGE IRHLFRSFAF LHCKKLVENG GMFVCKT RH LVLAGGSKPR DVTNFTVGGF APMSPRISSP MHPSAGGQRG GFGSPGGGSG GMSRGRGRRD NELIGQTVRI SQGPYKGY I GVVKDATEST ARVELHSTCQ TISVDRQRLT TVGSRRPGGM TSTYGRTPMY GSQTPMYGSG SRTPMYGSQT PLQDGSRTP HYGSQTPLHD GSRTPAQSGA WDPNNPNTPS RAEEEYEYAF DDEPTPSPQA YGGTPNPQTP GYPDPSSPQV NPQYNPQTPG TPAMYNTDQ FSPYAAPSPQ GSYQPSPSPQ SYHQVAPSPA GYQNTHSPAS YHPTPSPMAY QASPSPSPVG YSPMTPGAPS P GGYNPHTP GSGIEQNSSD WVTTDIQVKV RDTYLDTQVV GQTGVIRSVT GGMCSVYLKD SEKVVSISSE HLEPITPTKN NK VKVILGE DREATGVLLS IDGEDGIVRM DLDEQLKILN LRFLGKLLEA

UniProtKB: Transcription elongation factor SPT5

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Macromolecule #22: Integrator complex subunit 1

MacromoleculeName: Integrator complex subunit 1 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 244.776078 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMNRAKPTT VRRPSAAAKP SGHPPPGDFI ALGSKGQANE SKTASTLLKP APSGLPSERK RDAAAALSSA SALTGLTKRP KLSSTPPLS ALGRLAEAAV AEKRAISPSI KEPSVVPIEV LPTVLLDEIE AAELEGNDDR IEGVLCGAVK QLKVTRAKPD S TLYLSLMY ...String:
SNMNRAKPTT VRRPSAAAKP SGHPPPGDFI ALGSKGQANE SKTASTLLKP APSGLPSERK RDAAAALSSA SALTGLTKRP KLSSTPPLS ALGRLAEAAV AEKRAISPSI KEPSVVPIEV LPTVLLDEIE AAELEGNDDR IEGVLCGAVK QLKVTRAKPD S TLYLSLMY LAKIKPNIFA TEGVIEALCS LLRRDASINF KAKGNSLVSV LACNLLMAAY EEDENWPEIF VKVYIEDSLG ER IWVDSPH CKTFVDNIQT AFNTRMPPRS VLLQGEAGRV AGDLGAGSSP HPSLTEEEDS QTELLIAEEK LSPEQEGQLM PRY EELAES VEEYVLDMLR DQLNRRQPID NVSRNLLRLL TSTCGYKEVR LLAVQKLEMW LQNPKLTRPA QDLLMSVCMN CNTH GSEDM DVISHLIKIR LKPKVLLNHF MLCIRELLSA HKDNLGTTIK LVIFNELSSA RNPNNMQVLY TALQHSSELA PKFLA MVFQ DLLTNKDDYL RASRALLREI IKQTKHEINF QAFCLGLMQE RKEPQYLEME FKERFVVHIT DVLAVSMMLG ITAQVK EAG IAWDKGEKRN LEVLRSFQNQ IAAIQRDAVW WLHTVVPSIS KLAPKDYVHC LHKVLFTEQP ETYYKWDNWP PESDRNF FL RLCSEVPILE DTLMRILVIG LSRELPLGPA DAMELADHLV KRAAAVQADD VEVLKVGRTQ LIDAVLNLCT YHHPENIQ L PPGYQPPNLA ISTLYWKAWP LLLVVAAFNP ENIGLAAWEE YPTLKMLMEM VMTNNYSYPP CTLTDEETRT EMLNRELQT AQREKQEILA FEGHLAAAST KQTITESSSL LLSQLTSLDP QGPPRRPPPH ILDQVKSLNQ SLRLGHLLCR SRNPDFLLHI IQRQASSQS MPWLADLVQS SEGSLDVLPV QCLCEFLLHD AVDDAASGEE DDEGESKEQK AKKRQRQQKQ RQLLGRLQDL L LGPKADEQ TTCEVLDYFL RRLGSSQVAS RVLAMKGLSL VLSEGSLRDG EEKEPPMEED VGDTDVLQGY QWLLRDLPRL PL FDSVRST TALALQQAIH METDPQTISA YLIYLSQHTP VEEQAQHSDL ALDVARLVVE RSTIMSHLFS KLSPSAASDA VLS ALLSIF SRYVRRMRQS KEGEEVYSWS ESQDQVFLRW SSGETATMHI LVVHAMVILL TLGPPRADDS EFQALLDIWF PEEK PLPTA FLVDTSEEAL LLPDWLKLRM IRSEVLRLVD AALQDLEPQQ LLLFVQSFGI PVSSMSKLLQ FLDQAVAHDP QTLEQ NIMD KNYMAHLVEV QHERGASGGQ TFHSLLTASL PPRRDSTEAP KPKSSPEQPI GQGRIRVGTQ LRVLGPEDDL AGMFLQ IFP LSPDPRWQSS SPRPVALALQ QALGQELARV VQGSPEVPGI TVRVLQALAT LLSSPHGGAL VMSMHRSHFL ACPLLRQ LC QYQRCVPQDT GFSSLFLKVL LQMLQWLDSP GVEGGPLRAQ LRMLASQASA GRRLSDVRGG LLRLAEALAF RQDLEVVS S TVRAVIATLR SGEQCSVEPD LISKVLQGLI EVRSPHLEEL LTAFFSATAD AASPFPACKP VVVVSSLLLQ EEEPLAGGK PGADGGSLEA VRLGPSSGLL VDWLEMLDPE VVSSCPDLQL RLLFSRRKGK GQAQVPSFRP YLLTLFTHQS SWPTLHQCIR VLLGKSREQ RFDPSASLDF LWACIHVPRI WQGRDQRTPQ KRREELVLRV QGPELISLVE LILAEAETRS QDGDTAACSL I QARLPLLL SCCCGDDESV RKVTEHLSGC IQQWGDSVLG RRCRDLLLQL YLQRPELRVP VPEVLLHSEG AASSSVCKLD GL IHRFITL LADTSDSRAL ENRGADASMA CRKLAVAHPL LLLRHLPMIA ALLHGRTHLN FQEFRQQNHL SCFLHVLGLL ELL QPHVFR SEHQGALWDC LLSFIRLLLN YRKSSRHLAA FINKFVQFIH KYITYNAPAA ISFLQKHADP LHDLSFDNSD LVML KSLLA GLSLPSRDDR TDRGLDEEGE EESSAGSLPL VSVSLFTPLT AAEMAPYMKR LSRGQTVEDL LEVLSDIDEM SRRRP EILS FFSTNLQRLM SSAEECCRNL AFSLALRSMQ NSPSIAAAFL PTFMYCLGSQ DFEVVQTALR NLPEYALLCQ EHAAVL LHR AFLVGMYGQM DPSAQISEAL RILHMEAVM

UniProtKB: Integrator complex subunit 1

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Macromolecule #23: Integrator complex subunit 2

MacromoleculeName: Integrator complex subunit 2 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 134.451625 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MKDQQTVIMT ECTSLQFVSP FAFEAMQKVD VVCLASLSDP ELRLLLPCLV RMALCAPADQ SQSWAQDKKL ILRLLSGVEA VNSIVALLS VDFHALEQDA SKEQQLRHKL GGGSGESILV SQLQHGLTLE FEHSDSPRRL RLVLSELLAI MNKVSESNGE F FFKSSELF ...String:
MKDQQTVIMT ECTSLQFVSP FAFEAMQKVD VVCLASLSDP ELRLLLPCLV RMALCAPADQ SQSWAQDKKL ILRLLSGVEA VNSIVALLS VDFHALEQDA SKEQQLRHKL GGGSGESILV SQLQHGLTLE FEHSDSPRRL RLVLSELLAI MNKVSESNGE F FFKSSELF ESPVYLEEAA DVLCILQAEL PSLLPIVDVA EALLHVRNGA WFLCLLVANV PDSFNEVCRG LIKNGERQDE ES LGGRRRT DALRFLCKMN PSQALKVRGM VVEECHLPGL GVALTLDHTK NEACEDGVSD LVCFVSGLLL GTNAKVRTWF GTF IRNGQQ RKRETSSSVL WQMRRQLLLE LMGILPTVRS TRIVEEADVD MEPNVSVYSG LKEEHVVKAS ALLRLYCALM GIAG LKPTE EEAEQLLQLM TSRPPATPAG VRFVSLSFCM LLAFSTLVST PEQEQLMVVW LSWMIKEEAY FESTSGVSAS FGEML LLVA MYFHSNQLSA IIDLVCSTLG MKIVIKPSSL SRMKTIFTQE IFTEQVVTAH AVRVPVTSNL SANITGFLPI HCIYQL LRS RSFTKHKVSI KDWIYRQLCE TSTPLHPQLL PLIDVYINSI LTPASKSNPE ATNQPVTEQE ILNIFQGVIG GDNIRLN QR FSITAQLLVL YYILSYEEAL LANTKTLAAM QRKPKSYSSS LMDQIPIKFL IRQAQGLQQE LGGLHSALLR LLATNYPH L CIVDDWICEE EITGTDALLR RMLLTNNAKN HSPKQLQEAF SAVPVNNTQV MQIIEHLTLL SASELIPYAE VLTSNMSQL LNSGVPRRIL QTVNKLWMVL NTVMPRRLWV MTVNALQPSI KFVRQQKYTQ NDLMIDPLIV LRCDQRVHRC PPLMDITLHM LNGYLLASK AYLSAHLKET EQDRPSQNNT IGLVGQTDAP EVTREELKNA LLAAQDSAAV QILLEICLPT EEEKANGVNP D SLLRNVQS VITTSAPNKG MEEGEDNLLC NLREVQCLIC CLLHQMYIAD PNIAKLVHFQ GYPCELLPLT VAGIPSMHIC LD FIPELIA QPELEKQIFA IQLLSHLCIQ YALPKSLSVA RLAVNVMGTL LTVLTQAKRY AFFMPTLPSL VSFCRAFPPL YED IMSLLI QIGQVCASDV ATQTRDIDPI ITRLQQIKEK PSGWSQICKD SSYKNGSRDT GSMDPDVQLC HCIERTVIEI INMS VSGI

UniProtKB: Integrator complex subunit 2

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Macromolecule #24: Integrator complex subunit 4

MacromoleculeName: Integrator complex subunit 4 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 108.306758 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAAHLKKRVY EEFTKVVQPQ EEIATKKLRL TKPSKSAALH IDLCKATSPA DALQYLLQFA RKPVEAESVE GVVRILLEHY YKENDPSVR LKIASLLGLL SKTAGFSPDC IMDDAINILQ NEKSHQVLAQ LLDTLLAIGT KLPENQAIQM RLVDVACKHL T DTSHGVRN ...String:
MAAHLKKRVY EEFTKVVQPQ EEIATKKLRL TKPSKSAALH IDLCKATSPA DALQYLLQFA RKPVEAESVE GVVRILLEHY YKENDPSVR LKIASLLGLL SKTAGFSPDC IMDDAINILQ NEKSHQVLAQ LLDTLLAIGT KLPENQAIQM RLVDVACKHL T DTSHGVRN KCLQLLGNLG SLEKSVTKDA EGLAARDVQK IIGDYFSDQD PRVRTAAIKA MLQLHERGLK LHQTIYNQAC KL LSDDYEQ VRSAAVQLIW VVSQLYPESI VPIPSSNEEI RLVDDAFGKI CHMVSDGSWV VRVQAAKLLG SMEQVSSHFL EQT LDKKLM SDLRRKRTAH ERAKELYSSG EFSSGRKWGD DAPKEEVDTG AVNLIESGAC GAFVHGLEDE MYEVRIAAVE ALCM LAQSS PSFAEKCLDF LVDMFNDEIE EVRLQSIHTM RKISNNITLR EDQLDTVLAV LEDSSRDIRE ALHELLCCTN VSTKE GIHL ALVELLKNLT KYPTDRDSIW KCLKFLGSRH PTLVLPLVPE LLSTHPFFDT AEPDMDDPAY IAVLVLIFNA AKTCPT MPA LFSDHTFRHY AYLRDSLSHL VPALRLPGRK LVSSAVSPSI IPQEDPSQQF LQQSLERVYS LQHLDPQGAQ ELLEFTI RD LQRLGELQSE LAGVADFSAT YLRCQLLLIK ALQEKLWNVA APLYLKQSDL ASAAAKQIME ETYKMEFMYS GVENKQVV I IHHMRLQAKA LQLIVTARTT RGLDPLFGMC EKFLQEVDFF QRYFIADLPH LQDSFVDKLL DLMPRLMTSK PAEVVKILQ TMLRQSAFLH LPLPEQIHKA SATIIEPAGE SDNPLRFTSG LVVALDVDAT LEHVQDPQNT VKVQVLYPDG QAQMIHPKPA DFRNPGPGR HRLITQVYLS HTAWTEACQV EVRLLLAYNS SARIPKCPWM EGGEMSPQVE TSIEGTIPFS KPVKVYIMPK P ARR

UniProtKB: Integrator complex subunit 4

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Macromolecule #25: Integrator complex subunit 5

MacromoleculeName: Integrator complex subunit 5 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 108.316422 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMSALCDPP GAPGPPGPAP ATHGPAPLSA QELSQEIKAF LTGVDPILGH QLSAREHARC GLLLLRSLPP ARAAVLDHLR GVFDESVRA HLAALDETPV AGPPHLRPPP PSHVPAGGPG LEDVVQEVQQ VLSEFIRANP KAWAPVISAW SIDLMGQLSS T YSGQHQRV ...String:
SNMSALCDPP GAPGPPGPAP ATHGPAPLSA QELSQEIKAF LTGVDPILGH QLSAREHARC GLLLLRSLPP ARAAVLDHLR GVFDESVRA HLAALDETPV AGPPHLRPPP PSHVPAGGPG LEDVVQEVQQ VLSEFIRANP KAWAPVISAW SIDLMGQLSS T YSGQHQRV PHATGALNEL LQLWMGCRAT RTLMDIYVQC LSALIGSCPD ACVDALLDTS VQHSPHFDWV VAHIGSSFPG TI ISRVLSC GLKDFCVHGG AGGGAGSSGG SSSQTPSTDP FPGSPAIPAE KRVPKIASVV GILGHLASRH GDSIRRELLR MFH DSLAGG SGGRSGDPSL QATVPFLLQL AVMSPALLGT VSGELVDCLK PPAVLSQLQQ HLQGFPREEL DNMLNLAVHL VSQA SGAGA YRLLQFLVDT AMPASVITTQ GLAVPDTVRE ACDRLIQLLL LHLQKLVHHR GGSPGEGVLG PPPPPRLVPF LDALK NHVG ELCGETLRLE RKRFLWQHQL LGLLSVYTRP SCGPEALGHL LSRARSPEEL SLATQLYAGL VVSLSGLLPL AFRSCL ARV HAGTLQPPFT ARFLRNLALL VGWEQQGGEG PAALGAHFGE SASAHLSDLA PLLLHPEEEV AEAAASLLAI CPFPSEA LS PSQLLGLVRA GVHRFFASLR LHGPPGVASA CQLLTRLSQT SPAGLKAVLQ LLVEGALHRG NTELFGGQVD GDNETLSV V SASLASASLL DTNRRHTAAV PGPGGIWSVF HAGVIGRGLK PPKFVQSRNQ QEVIYNTQSL LSLLVHCCSA PGGTECGEC WGAPILSPEA AKAVAVTLVE SVCPDAAGAE LAWPPEEHAR ATVERDLRIG RRFREQPLLF ELLKLVAAAP PALCYCSVLL RGLLAALLG HWEASRHPDT THSPWHLEAS CTLVAVMAEG SLLPPALGNM HEVFSQLAPF EVRLLLLSVW GFLREHGPLP Q KFIFQSER GRFIRDFSRE GGGEGGPHLA VLHSVLHRNI DRLGLFSGRF QAPSPSTLLR QGT

UniProtKB: Integrator complex subunit 5

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Macromolecule #26: Integrator complex subunit 6

MacromoleculeName: Integrator complex subunit 6 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 100.72825 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMPILLFLI DTSASMNQRS HLGTTYLDTA KGAVETFMKL RARDPASRGD RYMLVTFEEP PYAIKAGWKE NHATFMNELK NLQAEGLTT LGQSLRTAFD LLNLNRLVTG IDNYGQGRNP FFLEPAIIIT ITDGSKLTTT SGVQDELHLP LNSPLPGSEL T KEPFRWDQ ...String:
SNMPILLFLI DTSASMNQRS HLGTTYLDTA KGAVETFMKL RARDPASRGD RYMLVTFEEP PYAIKAGWKE NHATFMNELK NLQAEGLTT LGQSLRTAFD LLNLNRLVTG IDNYGQGRNP FFLEPAIIIT ITDGSKLTTT SGVQDELHLP LNSPLPGSEL T KEPFRWDQ RLFALVLRLP GTMSVESEQL TGVPLDDSAI TPMCEVTGGR SYSVCSPRML NQCLESLVQK VQSGVVINFE KA GPDPSPV EDGQPDISRP FGSQPWHSCH KLIYVRPNPK TGVPIGHWPV PESFWPDQNS PTLPPRTSHP VVKFSCTDCE PMV IDKLPF DKYELEPSPL TQFILERKSP QTCWQVYVSN SAKYSELGHP FGYLKASTAL NCVNLFVMPY NYPVLLPLLD DLFK VHKAK PTLKWRQSFE SYLKTMPPYY LGPLKKAVRM MGAPNLIADS MEYGLSYSVI SYLKKLSQQA KIESDRVIGS VGKKV VQET GIKVRSRSHG LSMAYRKDFQ QLLQGISEDV PHRLLDLNMK EYTGFQVALL NKDLKPQTFR NAYDIPRRNL LDHLTR MRS NLLKSTRRFL KGQDEDQVHS VPIAQMGNYQ EYLKQVPSPL RELDPDQPRR LHTFGNPFKL DKKGMMIDEA DEFVAGP QN KHKRPGEPNM QGIPKRRRCM SPLLRGRQQN PVVNNHIGGK GPPAPTTQAQ PDLIKPLPLH KISETTNDSI IHDVVENH V ADQLSSDITP NAMDTEFSAS SPASLLERPT NHMEALGHDH LGTNDLTVGG FLENHEEPRD KEQCAEENIP ASSLNKGKK LMHCRSHEEV NTELKAQIMK EIRKPGRKYE RIFTLLKHVQ GSLQTRLIFL QNVIKEASRF KKRMLIEQLE NFLDEIHRRA NQINHINSN

UniProtKB: Integrator complex subunit 6

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Macromolecule #27: Integrator complex subunit 7

MacromoleculeName: Integrator complex subunit 7 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 107.153805 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMASNSTKS FLADAGYGEQ ELDANSALME LDKGLRSGKL GEQCEAVVRF PRLFQKYPFP ILINSAFLKL ADVFRVGNNF LRLCVLKVT QQSEKHLEKI LNVDEFVKRI FSVIHSNDPV ARAITLRMLG SLASIIPERK NAHHSIRQSL DSHDNVEVEA A VFAAANFS ...String:
SNMASNSTKS FLADAGYGEQ ELDANSALME LDKGLRSGKL GEQCEAVVRF PRLFQKYPFP ILINSAFLKL ADVFRVGNNF LRLCVLKVT QQSEKHLEKI LNVDEFVKRI FSVIHSNDPV ARAITLRMLG SLASIIPERK NAHHSIRQSL DSHDNVEVEA A VFAAANFS AQSKDFAVGI CNKISEMIQG LATPVDLKLK LIPILQHMHH DAILASSARQ LLQQLVTSYP STKMVIVSLH TF TLLAASS LVDTPKQIQL LLQYLKNDPR KAVKRLAIQD LKLLANKTPH TWSRENIQAL CECALQTPYD SLKLGMLSVL STL SGTIAI KHYFSIVPGN VSSSPRSSDL VKLAQECCYH NNRGIAAHGV RVLTNITVSC QEKDLLALEQ DAVFGLESLL VLCS QDDSP GAQATLKIAL NCMVKLAKGR PHLSQSVVET LLTQLHSAQD AARILMCHCL AAIAMQLPVL GDGMLGDLME LYKVI GRSA TDKQQELLVS LATVIFVASQ KALSVESKAV IKQQLESVSN GWTVYRIARQ ASRMGNHDMA KELYQSLLTQ VASEHF YFW LNSLKEFSHA EQCLTGLQEE NYSSALSCIA ESLKFYHKGI ASLTAASTPL NPLSFQCEFV KLRIDLLQAF SQLICTC NS LKTSPPPAIA TTIAMTLGND LQRCGRISNQ MKQSMEEFRS LASRYGDLYQ ASFDADSATL RNVELQQQSC LLISHAIE A LILDPESASF QEYGSTGTAH ADSEYERRMM SVYNHVLEEV ESLNRKYTPV SYMHTACLCN AIIALLKVPL SFQRYFFQK LQSTSIKLAL SPSPRNPAEP IAVQNNQQLA LKVEGVVQHG SKPGLFRKIQ SVCLNVSSTL QSKSGQDYKI PIDNMTNEME QRVEPHNDY FSTQFLLNFA ILGTHNITVE SSVKDANGIV WKTGPRTTIF VKSLEDPYSQ QIRLQQQQAQ QPLQQQQQRN A YTRF

UniProtKB: Integrator complex subunit 7

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Macromolecule #28: Integrator complex subunit 8

MacromoleculeName: Integrator complex subunit 8 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 113.219859 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSAEAADREA ATSSRPCTPP QTCWFEFLLE ESLLEKHLRK PCPDPAPVQL IVQFLEQASK PSVNEQNQVQ PPPDNKRNRI LKLLALKVA AHLKWDLDIL EKSLSVPVLN MLLNELLCIS KVPPGTKHVD MDLATLPPTT AMAVLLYNRW AIRTIVQSSF P VKQAKPGP ...String:
MSAEAADREA ATSSRPCTPP QTCWFEFLLE ESLLEKHLRK PCPDPAPVQL IVQFLEQASK PSVNEQNQVQ PPPDNKRNRI LKLLALKVA AHLKWDLDIL EKSLSVPVLN MLLNELLCIS KVPPGTKHVD MDLATLPPTT AMAVLLYNRW AIRTIVQSSF P VKQAKPGP PQLSVMNQMQ QEKELTENIL KVLKEQAADS ILVLEAALKL NKDLYVHTMR TLDLLAMEPG MVNGETESST AG LKVKTEE MQCQVCYDLG AAYFQQGSTN SAVYENAREK FFRTKELIAE IGSLSLHCTI DEKRLAGYCQ ACDVLVPSSD STS QQLTPY SQVHICLRSG NYQEVIQIFI EDNLTLSLPV QFRQSVLREL FKKAQQGNEA LDEICFKVCA CNTVRDILEG RTIS VQFNQ LFLRPNKEKI DFLLEVCSRS VNLEKASESL KGNMAAFLKN VCLGLEDLQY VFMISSHELF ITLLKDEERK LLVDQ MRKR SPRVNLCIKP VTSFYDIPAS ASVNIGQLEH QLILSVDPWR IRQILIELHG MTSERQFWTV SNKWEVPSVY SGVILG IKD NLTRDLVYIL MAKGLHCSTV KDFSHAKQLF AACLELVTEF SPKLRQVMLN EMLLLDIHTH EAGTGQAGER PPSDLIS RV RGYLEMRLPD IPLRQVIAEE CVAFMLNWRE NEYLTLQVPA FLLQSNPYVK LGQLLAATCK ELPGPKESRR TAKDLWEV V VQICSVSSQH KRGNDGRVSL IKQRESTLGI MYRSELLSFI KKLREPLVLT IILSLFVKLH NVREDIVNDI TAEHISIWP SSIPNLQSVD FEAVAITVKE LVRYTLSINP NNHSWLIIQA DIYFATNQYS AALHYYLQAG AVCSDFFNKA VPPDVYTDQV IKRMIKCCS LLNCHTQVAI LCQFLREIDY KTAFKSLQEQ NSHDAMDSYY DYIWDVTILE YLTYLHHKRG ETDKRQIAIK A IGQTELNA SNPEEVLQLA AQRRKKKFLQ AMAKLYF

UniProtKB: Integrator complex subunit 8

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Macromolecule #29: Integrator complex subunit 9

MacromoleculeName: Integrator complex subunit 9 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 73.891219 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MKLYCLSGHP TLPCNVLKFK STTIMLDCGL DMTSTLNFLP LPLVQSPRLS NLPGWSLKDG NAFLDKELKE CSGHVFVDSV PEFCLPETE LIDLSTVDVI LISNYHCMMA LPYITEHTGF TGTVYATEPT VQIGRLLMEE LVNFIERVPK AQSASLWKNK D IQRLLPSP ...String:
MKLYCLSGHP TLPCNVLKFK STTIMLDCGL DMTSTLNFLP LPLVQSPRLS NLPGWSLKDG NAFLDKELKE CSGHVFVDSV PEFCLPETE LIDLSTVDVI LISNYHCMMA LPYITEHTGF TGTVYATEPT VQIGRLLMEE LVNFIERVPK AQSASLWKNK D IQRLLPSP LKDAVEVSTW RRCYTMQEVN SALSKIQLVG YSQKIELFGA VQVTPLSSGY ALGSSNWIIQ SHYEKVSYVS GS SLLTTHP QPMDQASLKN SDVLVLTGLT QIPTANPDGM VGEFCSNLAL TVRNGGNVLV PCYPSGVIYD LLECLYQYID SAG LSSVPL YFISPVANSS LEFSQIFAEW LCHNKQSKVY LPEPPFPHAE LIQTNKLKHY PSIHGDFSND FRQPCVVFTG HPSL RFGDV VHFMELWGKS SLNTVIFTEP DFSYLEALAP YQPLAMKCIY CPIDTRLNFI QVSKLLKEVQ PLHVVCPEQY TQPPP AQSH RMDLMIDCQP PAMSYRRAEV LALPFKRRYE KIEIMPELAD SLVPMEIKPG ISLATVSAVL HTKDNKHLLQ PPPRPA QPT SGKKRKRVSD DVPDCKVLKP LLSGSIPVEQ FVQTLEKHGF SDIKVEDTAK GHIVLLQEAE TLIQIEEDST HIICDND EM LRVRLRDLVL KFLQKF

UniProtKB: Integrator complex subunit 9

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Macromolecule #30: Integrator complex subunit 10

MacromoleculeName: Integrator complex subunit 10 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 82.339867 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSAQGDCEFL VQRARELVPQ DLWAAKAWLI TARSLYPADF NIQYEMYTIE RNAERTATAG RLLYDMFVNF PDQPVVWREI SIITSALRN DSQDKQTQFL RSLFETLPGR VQCEMLLKVT EQCFNTLERS EMLLLLLRRF PETVVQHGVG LGEALLEAET I EEQESPVN ...String:
MSAQGDCEFL VQRARELVPQ DLWAAKAWLI TARSLYPADF NIQYEMYTIE RNAERTATAG RLLYDMFVNF PDQPVVWREI SIITSALRN DSQDKQTQFL RSLFETLPGR VQCEMLLKVT EQCFNTLERS EMLLLLLRRF PETVVQHGVG LGEALLEAET I EEQESPVN CFRKLFVCDV LPLIINNHDV RLPANLLYKY LNKAAEFYIN YVTRSTQIEN QHQGAQDTSD LMSPSKRSSQ KY IIEGLTE KSSQIVDPWE RLFKILNVVG MRCEWQMDKG RRSYGDILHR MKDLCRYMNN FDSEAHAKYK NQVVYSTMLV FFK NAFQYV NSIQPSLFQG PNAPSQVPLV LLEDVSNVYG DVEIDRNKHI HKKRKLAEGR EKTMSSDDED CSAKGRNRHI VVNK AELAN STEVLESFKL ARESWELLYS LEFLDKEFTR ICLAWKTDTW LWLRIFLTDM IIYQGQYKKA IASLHHLAAL QGSIS QPQI TGQGTLEHQR ALIQLATCHF ALGEYRMTCE KVLDLMCYMV LPIQDGGKSQ EEPSKVKPKF RKGSDLKLLP CTSKAI MPY CLHLMLACFK LRAFTDNRDD MALGHVIVLL QQEWPRGENL FLKAVNKICQ QGNFQYENFF NYVTNIDMLE EFAYLRT QE GGKIHLELLP NQGMLIKHHT VTRGITKGVK EDFRLAMERQ VSRCGENLMV VLHRFCINEK ILLLQTLT

UniProtKB: Integrator complex subunit 10

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Macromolecule #31: Integrator complex subunit 11

MacromoleculeName: Integrator complex subunit 11 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 67.95675 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMPEIRVTP LGAGQDVGRS CILVSIAGKN VMLDCGMHMG FNDDRRFPDF SYITQNGRLT DFLDCVIISH FHLDHCGALP YFSEMVGYD GPIYMTHPTQ AICPILLEDY RKIAVDKKGE ANFFTSQMIK DCMKKVVAVH LHQTVQVDDE LEIKAYYAGH V LGAAMFQI ...String:
SNMPEIRVTP LGAGQDVGRS CILVSIAGKN VMLDCGMHMG FNDDRRFPDF SYITQNGRLT DFLDCVIISH FHLDHCGALP YFSEMVGYD GPIYMTHPTQ AICPILLEDY RKIAVDKKGE ANFFTSQMIK DCMKKVVAVH LHQTVQVDDE LEIKAYYAGH V LGAAMFQI KVGSESVVYT GDYNMTPDRH LGAAWIDKCR PNLLITQSTY ATTIRDSKRC RERDFLKKVH ETVERGGKVL IP VFALGRA QELCILLETF WERMNLKVPI YFSTGLTEKA NHYYKLFIPW TNQKIRKTFV QRNMFEFKHI KAFDRAFADN PGP MVVFAT PGMLHAGQSL QIFRKWAGNE KNMVIMPGYC VQGTVGHKIL SGQRKLEMEG RQVLEVKMQV EYMSFSAHAD AKGI MQLVG QAEPESVLLV HGEAKKMEFL KQKIEQELRV NCYMPANGET VTLPTSPSIP VGISLGLLKR EMAQGLLPEA KKPRL LHGT LIMKDSNFRL VSSEQALKEL GLAEHQLRFT CRVHLHDTRK EQETALRVYS HLKSVLKDHC VQHLPDGSVT VESVLL QAA APSEDPGTKV LLVSWTYQDE ELGSFLTSLL KKGLPQAPS

UniProtKB: Integrator complex subunit 11

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Macromolecule #32: Integrator complex subunit 13

MacromoleculeName: Integrator complex subunit 13 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 80.34518 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MKIFSESHKT VFVVDHCPYM AESCRQHVEF DMLVKNRTQG IIPLAPISKS LWTCSVESSM EYCRIMYDIF PFKKLVNFIV SDSGAHVLN SWTQEDQNLQ ELMAALAAVG PPNPRADPEC CSILHGLVAA VETLCKITEY QHEARTLLME NAERVGNRGR I ICITNAKS ...String:
MKIFSESHKT VFVVDHCPYM AESCRQHVEF DMLVKNRTQG IIPLAPISKS LWTCSVESSM EYCRIMYDIF PFKKLVNFIV SDSGAHVLN SWTQEDQNLQ ELMAALAAVG PPNPRADPEC CSILHGLVAA VETLCKITEY QHEARTLLME NAERVGNRGR I ICITNAKS DSHVRMLEDC VQETIHEHNK LAANSDHLMQ IQKCELVLIH TYPVGEDSLV SDRSKKELSP VLTSEVHSVR AG RHLATKL NILVQQHFDL ASTTITNIPM KEEQHANTSA NYDVELLHHK DAHVDFLKSG DSHLGGGSRE GSFKETITLK WCT PRTNNI ELHYCTGAYR ISPVDVNSRP SSCLTNFLLN GRSVLLEQPR KSGSKVISHM LSSHGGEIFL HVLSSSRSIL EDPP SISEG CGGRVTDYRI TDFGEFMREN RLTPFLDPRY KIDGSLEVPL ERAKDQLEKH TRYWPMIISQ TTIFNMQAVV PLASV IVKE SLTEEDVLNC QKTIYNLVDM ERKNDPLPIS TVGTRGKGPK RDEQYRIMWN ELETLVRAHI NNSEKHQRVL ECLMAC RSK PPEEEERKKR GRKREDKEDK SEKAVKDYEQ EKSWQDSERL KGILERGKEE LAEAEIIKDS PDSPEPPNKK PLVEMDE TP QVEKSKGPVS LLSLWSNRIN TANSRKHQEF AGRLNSVNNR AELYQHLKEE NGMETTENGK ASRQ

UniProtKB: Integrator complex subunit 13

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Macromolecule #33: Integrator complex subunit 14

MacromoleculeName: Integrator complex subunit 14 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 57.526547 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MPTVVVMDVS LSMTRPVSIE GSEEYQRKHL AAHGLTMLFE HMATNYKLEF TALVVFSSLW ELMVPFTRDY NTLQEALSNM DDYDKTCLE SALVGVCNIV QQEWGGAIPC QVVLVTDGCL GIGRGSLRHS LATQNQRSES NRFPLPFPFP SKLYIMCMAN L EELQSTDS ...String:
MPTVVVMDVS LSMTRPVSIE GSEEYQRKHL AAHGLTMLFE HMATNYKLEF TALVVFSSLW ELMVPFTRDY NTLQEALSNM DDYDKTCLE SALVGVCNIV QQEWGGAIPC QVVLVTDGCL GIGRGSLRHS LATQNQRSES NRFPLPFPFP SKLYIMCMAN L EELQSTDS LECLERLIDL NNGEGQIFTI DGPLCLKNVQ SMFGKLIDLA YTPFHAVLKC GHLTADVQVF PRPEPFVVDE EI DPIPKVI NTDLEIVGFI DIADISSPPV LSRHLVLPIA LNKEGDEVGT GITDDNEDEN SANQIAGKIP NFCVLLHGSL KVE GMVAIV QLGPEWHGML YSQADSKKKS NLMMSLFEPG PEPLPWLGKM AQLGPISDAK ENPYGEDDNK SPFPLQPKNK RSYA QNVTV WIKPSGLQTD VQKILRNARK LPEKTQTFYK ELNRLRKAAL AFGFLDLLKG VADMLERECT LLPETAHPDA AFQLT HAAQ QLKLASTGTS EYAAYDQNIT PLHTDFSGSS TERI

UniProtKB: Integrator complex subunit 14

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Macromolecule #34: Integrator complex subunit 15

MacromoleculeName: Integrator complex subunit 15 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 50.303582 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMSDIRHSL LRRDALSAAK EVLYHLDIYF SSQLQSAPLP IVDKGPVELL EEFVFQVPKE RSAQPKRLNS LQELQLLEIM CNYFQEQTK DSVRQIIFSS LFSPQGNKAD DSRMSLLGKL VSMAVAVCRI PVLECAASWL QRTPVVYCVR LAKALVDDYC C LVPGSIQT ...String:
SNMSDIRHSL LRRDALSAAK EVLYHLDIYF SSQLQSAPLP IVDKGPVELL EEFVFQVPKE RSAQPKRLNS LQELQLLEIM CNYFQEQTK DSVRQIIFSS LFSPQGNKAD DSRMSLLGKL VSMAVAVCRI PVLECAASWL QRTPVVYCVR LAKALVDDYC C LVPGSIQT LKQIFSASPR FCCQFITSVT ALYDLSSDDL IPPMDLLEMI VTWIFEDPRL ILITFLNTPI AANLPIGFLE LT PLVGLIR WCVKAPLAYK RKKKPPLSNG HVSNKVTKDP GVGMDRDSHL LYSKLHLSVL QVLMTLQLHL TEKNLYGRLG LIL FDHMVP LVEEINRLAD ELNPLNASQE IELSLDRLAQ ALQVAMASGA LLCTRDDLRT LCSRLPHNNL LQLVISGPVQ QSPH AALPP GFYPHIHTPP LGYGAVPAHP AAHPALPTHP GHTFISGVTF PFRPIR

UniProtKB: Integrator complex subunit 15

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Macromolecule #35: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit...

MacromoleculeName: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 65.579531 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMAAADGDD SLYPIAVLID ELRNEDVQLR LNSIKKLSTI ALALGVERTR SELLPFLTDT IYDEDEVLLA LAEQLGTFTT LVGGPEYVH CLLPPLESLA TVEETVVRDK AVESLRAISH EHSPSDLEAH FVPLVKRLAG GDWFTSRTSA CGLFSVCYPR V SSAVKAEL ...String:
SNMAAADGDD SLYPIAVLID ELRNEDVQLR LNSIKKLSTI ALALGVERTR SELLPFLTDT IYDEDEVLLA LAEQLGTFTT LVGGPEYVH CLLPPLESLA TVEETVVRDK AVESLRAISH EHSPSDLEAH FVPLVKRLAG GDWFTSRTSA CGLFSVCYPR V SSAVKAEL RQYFRNLCSD DTPMVRRAAA SKLGEFAKVL ELDNVKSEII PMFSNLASDE QDSVRLLAVE ACVNIAQLLP QE DLEALVM PTLRQAAEDK SWRVRYMVAD KFTELQKAVG PEITKTDLVP AFQNLMKDCE AEVRAAASHK VKEFCENLSA DCR ENVIMS QILPCIKELV SDANQHVKSA LASVIMGLSP ILGKDNTIEH LLPLFLAQLK DECPEVRLNI ISNLDCVNEV IGIR QLSQS LLPAIVELAE DAKWRVRLAI IEYMPLLAGQ LGVEFFDEKL NSLCMAWLVD HVYAIREAAT SNLKKLVEKF GKEWA HATI IPKVLAMSGD PNYLHRMTTL FCINVLSEVC GQDITTKHML PTVLRMAGDP VANVRFNVAK SLQKIGPILD NSTLQS EVK PILEKLTQDQ DVDVKYFAQE ALTVLSLA

UniProtKB: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

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Macromolecule #36: Serine/threonine-protein phosphatase 2A catalytic subunit alpha i...

MacromoleculeName: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 35.836348 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMDEKVFTK ELDQWIEQLN ECKQLSESQV KSLCEKAKEI LTKESNVQEV RCPVTVCGDV HGQFHDLMEL FRIGGKSPDT NYLFMGDYV NRGYYSVETV TLLVALKVRY RERITILRGN HESRQITQVY GFYDECLRKY GNANVWKYFT DLFDYLPLTA L VDGQIFCL ...String:
SNMDEKVFTK ELDQWIEQLN ECKQLSESQV KSLCEKAKEI LTKESNVQEV RCPVTVCGDV HGQFHDLMEL FRIGGKSPDT NYLFMGDYV NRGYYSVETV TLLVALKVRY RERITILRGN HESRQITQVY GFYDECLRKY GNANVWKYFT DLFDYLPLTA L VDGQIFCL HGGLSPSIDT LDHIRALDRL QEVPHEGPMC DLLWSDPDDR GGWGISPRGA GYTFGQDISE TFNHANGLTL VS RAHQLVM EGYNWCHDRN VVTIFSAPNY CYRCGNQAAI MELDDTLKYS FLQFDPAPRR GEPHVTRRTP DYFL

UniProtKB: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

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Macromolecule #37: DSS1

MacromoleculeName: DSS1 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trichoplusia ni (cabbage looper)
Molecular weightTheoretical: 3.558796 KDa
SequenceString:
DVSVWEENWE DDIVQDDFNQ QLRLEMFR

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Macromolecule #38: Negative elongation factor A

MacromoleculeName: Negative elongation factor A / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 57.343598 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MASMRESDTG LWLHNKLGAT DELWAPPSIA SLLTAAVIDN IRLCFHGLSS AVKLKLLLGT LHLPRRTVDE MKGALMEIIQ LASLDSDPW VLMVADILKS FPDTGSLNLE LEEQNPNVQD ILGELREKVG ECEASAMLPL ECQYLNKNAL TTLAGPLTPP V KHFQLKRK ...String:
MASMRESDTG LWLHNKLGAT DELWAPPSIA SLLTAAVIDN IRLCFHGLSS AVKLKLLLGT LHLPRRTVDE MKGALMEIIQ LASLDSDPW VLMVADILKS FPDTGSLNLE LEEQNPNVQD ILGELREKVG ECEASAMLPL ECQYLNKNAL TTLAGPLTPP V KHFQLKRK PKSATLRAEL LQKSTETAQQ LKRSAGVPFH AKGRGLLRKM DTTTPLKGIP KQAPFRSPTA PSVFSPTGNR TP IPPSRTL LRKERGVKLL DISELDMVGA GREAKRRRKT LDAEVVEKPA KEETVVENAT PDYAAGLVST QKLGSLNNEP ALP STSYLP STPSVVPASS YIPSSETPPA PSSREASRPP EEPSAPSPTL PAQFKQRAPM YNSGLSPATP TPAAPTSPLT PTTP PAVAP TTQTPPVAMV APQTQAPAQQ QPKKNLSLTR EQMFAAQEMF KTANKVTRPE KALILGFMAG SRENPCQEQG DVIQI KLSE HTEDLPKADG QGSTTMLVDT VFEMNYATGQ WTRFKKYKPM TNVS

UniProtKB: Negative elongation factor A

+
Macromolecule #39: NELF-B,Negative elongation factor B,Negative elongation factor B,...

MacromoleculeName: NELF-B,Negative elongation factor B,Negative elongation factor B,Negative elongation factor B,Negative elongation factor B,Negative elongation factor B
type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 68.58768 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)MFAGL QDLGVANGED ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)MFAGL QDLGVANGED LKETLTNCTE PLKAIEQFQT ENGVLLPSLQ SALPFLDLHG TPRLEFHQSV FDELRDKLLE RVSAIASEG KAEERYKKLE DLLEKSFSLV KMPSLQPVVM CVMKHLPKVP EKKLKLVMAD KELYRACAVE VKRQIWQDNQ A LFGDEVSP LLKQYILEKE SALFSTELSV LHNFFSPSPK TRRQGEVVQR LTRMVGKNVK LYDMVLQFLR TLFLRTRNVH YC TLRAELL MSLHDLDVGE ICTVDPCHKF TWCLDACIRE RFVDSKRARE LQGFLDGVKK GQEQVLGDLS MILCDPFAIN TLA LSTVRH LQELVGQETL PRDSPDLLLL LRLLALGQGA WDMIDSQVFK EPKMEVELIT RFLPMLMSFL VDDYTFNVDQ KLPA EEKAP VSYPNTLPES FTKFLQEQRM ACEVGLYYVL HITKQRNKNA LLRLLPGLVE TFGDLAFGDI FLHLLTGNLA LLADE FALE DFCSSLFDGF FLTASPRKEN VHRHALRLLI HLHPRVAPSK LEALQKALEP TGQSGEAVKE LYSQLGEKLE QLDHRK PSP AQAAETPALE LPLPSVPAPA PL

UniProtKB: Negative elongation factor B

+
Macromolecule #40: Negative elongation factor C/D

MacromoleculeName: Negative elongation factor C/D / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 65.748586 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNMDEDYYGS AAEWGDEADG GQQEDDSGEG EDDAEVQQEC LHKFSTRDYI MEPSIFNTLK RYFQAGGSPE NVIQLLSENY TAVAQTVNL LAEWLIQTGV EPVQVQETVE NHLKSLLIKH FDPRKADSIF TEEGETPAWL EQMIAHTTWR DLFYKLAEAH P DCLMLNFT ...String:
SNMDEDYYGS AAEWGDEADG GQQEDDSGEG EDDAEVQQEC LHKFSTRDYI MEPSIFNTLK RYFQAGGSPE NVIQLLSENY TAVAQTVNL LAEWLIQTGV EPVQVQETVE NHLKSLLIKH FDPRKADSIF TEEGETPAWL EQMIAHTTWR DLFYKLAEAH P DCLMLNFT VKLISDAGYQ GEITSVSTAC QQLEVFSRVL RTSLATILDG GEENLEKNLP EFAKMVCHGE HTYLFAQAMM SV LAQEEQG GSAVRRIAQE VQRFAQEKGH DASQITLALG TAASYPRACQ ALGAMLSKGA LNPADITVLF KMFTSMDPPP VEL IRVPAF LDLFMQSLFK PGARINQDHK HKYIHILAYA ASVVETWKKN KRVSINKDEL KSTSKAVETV HNLCCNENKG ASEL VAELS TLYQCIRFPV VAMGVLKWVD WTVSEPRYFQ LQTDHTPVHL ALLDEISTCH QLLHPQVLQL LVKLFETEHS QLDVM EQLE LKKTLLDRMV HLLSRGYVLP VVSYIRKCLE KLDTDISLIR YFVTEVLDVI APPYTSDFVQ LFLPILENDS IAGTIK TEG EHDPVTEFIA HCKSNFIMVN

UniProtKB: Negative elongation factor C/D

+
Macromolecule #41: UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-U...

MacromoleculeName: UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK
type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 1.890321 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

+
Macromolecule #15: non-template DNA

MacromoleculeName: non-template DNA / type: dna / ID: 15 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 45.938262 KDa
SequenceString: (DG)(DA)(DT)(DA)(DT)(DC)(DG)(DA)(DT)(DG) (DT)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DT)(DG) (DA)(DC)(DA)(DC)(DG)(DT)(DG)(DC)(DC) (DT)(DG)(DG)(DA)(DG)(DA)(DC)(DT)(DA)(DG) (DG) (DG)(DA)(DG)(DT)(DA)(DA) ...String:
(DG)(DA)(DT)(DA)(DT)(DC)(DG)(DA)(DT)(DG) (DT)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DT)(DG) (DA)(DC)(DA)(DC)(DG)(DT)(DG)(DC)(DC) (DT)(DG)(DG)(DA)(DG)(DA)(DC)(DT)(DA)(DG) (DG) (DG)(DA)(DG)(DT)(DA)(DA)(DT)(DC) (DC)(DC)(DC)(DT)(DT)(DG)(DG)(DC)(DG)(DG) (DT)(DT) (DA)(DA)(DA)(DA)(DC)(DG)(DC) (DG)(DG)(DG)(DG)(DG)(DA)(DC)(DA)(DG)(DC) (DG)(DC)(DG) (DT)(DA)(DC)(DG)(DT)(DG) (DC)(DG)(DT)(DT)(DT)(DA)(DA)(DG)(DC)(DG) (DG)(DT)(DG)(DC) (DT)(DA)(DG)(DA)(DG) (DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC)(DG)(DA) (DC)(DC)(DA)(DA)(DT) (DT)(DG)(DA)(DG) (DC)(DG)(DG)(DC)(DC)(DT)(DC)(DG)(DG)(DC) (DA)(DC)(DC)(DG)(DG)(DG) (DA)(DT)(DT) (DC)(DT)(DG)(DA)(DT)

+
Macromolecule #20: Template DNA

MacromoleculeName: Template DNA / type: dna / ID: 20 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 56.757223 KDa
SequenceString: (DA)(DT)(DC)(DA)(DG)(DA)(DA)(DT)(DC)(DC) (DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG) (DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA) (DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG) (DA) (DC)(DA)(DG)(DC)(DT)(DC) ...String:
(DA)(DT)(DC)(DA)(DG)(DA)(DA)(DT)(DC)(DC) (DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG) (DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA) (DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG) (DA) (DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA) (DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA) (DA)(DA) (DC)(DG)(DC)(DA)(DC)(DG)(DT) (DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC) (DC)(DC)(DC) (DC)(DG)(DC)(DG)(DT)(DT) (DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA) (DA)(DG)(DG)(DG) (DG)(DA)(DT)(DT)(DA) (DC)(DT)(DC)(DC)(DC)(DT)(DA)(DG)(DT)(DC) (DT)(DC)(DC)(DA)(DG) (DG)(DC)(DA)(DC) (DG)(DT)(DG)(DT)(DC)(DA)(DG)(DA)(DT)(DA) (DT)(DA)(DT)(DA)(DC)(DA) (DT)(DC)(DG) (DA)(DT)(DA)(DT)(DC)(DG)(DG)(DC)(DC)(DA) (DC)(DC)(DA)(DC)(DC)(DC)(DA) (DG)(DA) (DC)(DA)(DC)(DA)(DC)(DC)(DA)(DA)(DA)(DC) (DA)(DC)(DA)(DA)(DG)(DA)(DC)(DA) (DG) (DT)(DG)(DA)(DT)

+
Macromolecule #17: RNA

MacromoleculeName: RNA / type: rna / ID: 17 / Number of copies: 1
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 5.549278 KDa
SequenceString:
GUGUGUCUGG GUGGUGG

+
Macromolecule #42: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 42 / Number of copies: 10 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #43: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 43 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #44: MANGANESE (II) ION

MacromoleculeName: MANGANESE (II) ION / type: ligand / ID: 44 / Number of copies: 2 / Formula: MN
Molecular weightTheoretical: 54.938 Da

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

Concentration0.3 mg/mL
BufferpH: 7.4
GridModel: Qu / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 40.44 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Startup modelType of model: INSILICO MODEL
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: NOT APPLICABLE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 80717
FSC plot (resolution estimation)

-
Atomic model buiding 1

Initial modelPDB ID:

7pks


Chain - Source name: PDB / Chain - Initial model type: experimental model
Output model

PDB-8rbx:
Structure of Integrator-PP2A bound to a paused RNA polymerase II-DSIF-NELF-nucleosome complex

-
Atomic model buiding 2

Initial modelPDB ID:

7ohc


Chain - Source name: PDB / Chain - Initial model type: experimental model
Output model

PDB-8rbx:
Structure of Integrator-PP2A bound to a paused RNA polymerase II-DSIF-NELF-nucleosome complex

-
Atomic model buiding 3

Initial modelPDB ID:

6sn1


Chain - Source name: PDB / Chain - Initial model type: experimental model
Output model

PDB-8rbx:
Structure of Integrator-PP2A bound to a paused RNA polymerase II-DSIF-NELF-nucleosome complex

-
Atomic model buiding 4

Initial modelChain - Source name: AlphaFold / Chain - Initial model type: in silico model
Output model

PDB-8rbx:
Structure of Integrator-PP2A bound to a paused RNA polymerase II-DSIF-NELF-nucleosome complex

+
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