+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDBU9 |
---|---|
Sample | Terminally truncated human βB2-crystallin
|
Function / homology | Function and homology information structural constituent of eye lens / lens development in camera-type eye / response to stimulus / visual perception / structural molecule activity / identical protein binding Similarity search - Function |
Biological species | Homo sapiens (human) |
Citation | Journal: Structure / Year: 2017 Title: Human βB2-Crystallin Forms a Face-en-Face Dimer in Solution: An Integrated NMR and SAXS Study. Authors: Zhaoyong Xi / Matthew J Whitley / Angela M Gronenborn / Abstract: βγ-Crystallins are long-lived eye lens proteins that are crucial for lens transparency and refractive power. Each βγ-crystallin comprises two homologous domains, which are connected by a short ...βγ-Crystallins are long-lived eye lens proteins that are crucial for lens transparency and refractive power. Each βγ-crystallin comprises two homologous domains, which are connected by a short linker. γ-Crystallins are monomeric, while β-crystallins crystallize as dimers and multimers. In the crystal, human βB2-crystallin is a domain-swapped dimer while the N-terminally truncated βB1-crystallin forms a face-en-face dimer. Combining and integrating data from multi-angle light scattering, nuclear magnetic resonance, and small-angle X-ray scattering of full-length and terminally truncated human βB2-crystallin in solution, we show that both these βB2-crystallin proteins are dimeric, possess C2 symmetry, and are more compact than domain-swapped dimers. Importantly, no inter-molecular paramagnetic relaxation enhancement effects compatible with domain swapping were detected. Our collective experimental results unambiguously demonstrate that, in solution, human βB2-crystallin is not domain swapped and exhibits a face-en-face dimer structure similar to the crystal structure of truncated βB1-crystallin. |
Contact author |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Data source
SASBDB page | SASDBU9 |
---|
-Related structure data
Related structure data | C: citing same article (ref.) |
---|---|
Similar structure data |
-External links
Related items in Molecule of the Month |
---|
-Models
Model #1128 | Type: dummy / Software: DAMMIN / Radius of dummy atoms: 2.00 A / Chi-square value: 0.216 / P-value: 0.038400 Search similar-shape structures of this assembly by Omokage search (details) |
---|
-Sample
Sample | Name: Terminally truncated human βB2-crystallin / Specimen concentration: 1.75 mg/ml |
---|---|
Buffer | Name: 25 mM NaPi, 5 mM DTT, 1 mM EDTA, / pH: 6.5 |
Entity #587 | Type: protein / Description: Beta-crystallin B2 / Formula weight: 20.911 / Num. of mol.: 2 / Source: Homo sapiens / References: UniProt: P43320 Sequence: MLNPKIIIFE QENFQGHSHE LNGPCPNLKE TGVEKAGSVL VQAGPWVGYE QANCKGEQFV FEKGEYPRWD SWTSSRRTDS LSSLRPIKVD SQEHKIILYE NPNFTGKKME IIDDDVPSFH AHGYQEKVSS VRVQSGTWVG YQYPGYRGLQ YLLEKGDYKD SSDFGAPHPQ VQSVRRIRDM QW |
-Experimental information
Beam | Instrument name: Advanced Photon Source (APS) 12ID-B SAXS/WAXS City: Argonne, IL / 国: USA / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.08856 Å / Dist. spec. to detc.: 2 mm | |||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Detector | Name: Pilatus 2M | |||||||||||||||||||||||||||||||||
Scan | Title: terminally truncated human βB2-crystallin / Measurement date: Mar 20, 2015 / Exposure time: 0.4 sec. / Number of frames: 30 / Unit: 1/A /
| |||||||||||||||||||||||||||||||||
Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 167 /
| |||||||||||||||||||||||||||||||||
Result | Type of curve: single_conc /
|