Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural insights into the assembly of gp130 family cytokine signaling complexes.
Journal, issue, pages	Sci Adv, Vol. 9, Issue 11, Page eade4395, Year 2023
Publish date	Mar 15, 2023
Authors	Yi Zhou / Panayiotis E Stevis / Jing Cao / Kei Saotome / Jiaxi Wu / Arielle Glatman Zaretsky / Sokol Haxhinasto / George D Yancopoulos / Andrew J Murphy / Mark W Sleeman / William C Olson / Matthew C Franklin /
PubMed Abstract	The interleukin-6 (IL-6) family cytokines signal through gp130 receptor homodimerization or heterodimerization with a second signaling receptor and play crucial roles in various cellular processes. ...The interleukin-6 (IL-6) family cytokines signal through gp130 receptor homodimerization or heterodimerization with a second signaling receptor and play crucial roles in various cellular processes. We determined cryo-electron microscopy structures of five signaling complexes of this family, containing full receptor ectodomains bound to their respective ligands ciliary neurotrophic factor, cardiotrophin-like cytokine factor 1 (CLCF1), leukemia inhibitory factor, IL-27, and IL-6. Our structures collectively reveal similarities and differences in the assembly of these complexes. The acute bends at both signaling receptors in all complexes bring the membrane-proximal domains to a ~30 angstrom range but with distinct distances and orientations. We also reveal how CLCF1 engages its secretion chaperone cytokine receptor-like factor 1. Our data provide valuable insights for therapeutically targeting gp130-mediated signaling.
External links	Sci Adv / PubMed:36930708 / PubMed Central
Methods	EM (single particle)
Resolution	2.93 - 4.14 Å
Structure data	27221 8d6a EMDB-27221: Cryo-EM map of human LIF signaling complex with full extracellular domains PDB-8d6a: Cryo-EM structure of human LIF signaling complex: model containing the interaction core region Method: EM (single particle) / Resolution: 3.54 Å 27227 8d74 EMDB-27227: Cryo-EM map of human CNTF signaling complex with full extracellular domains: refined from selected full particle dataset, with better resolution in the interaction core region PDB-8d74: Cryo-EM structure of human CNTF signaling complex: model containing the interaction core region Method: EM (single particle) / Resolution: 3.03 Å 27228 8d7e EMDB-27228, PDB-8d7e: Cryo-EM structure of human CNTFR alpha in complex with the Fab fragments of two antibodies Method: EM (single particle) / Resolution: 2.93 Å EMDB-27229: Cryo-EM map of human CNTF signaling complex with full extracellular domains: refined from a particle subset, with lower global resolution but improved LIFR D6-D8 density Method: EM (single particle) / Resolution: 3.37 Å 27230 8d7h EMDB-27230, PDB-8d7h: Cryo-EM structure of human CLCF1 in complex with CRLF1 and CNTFR alpha Method: EM (single particle) / Resolution: 3.4 Å 27231 8d7r EMDB-27231: Cryo-EM map of human CLCF1 signaling complex with full extracellular domains PDB-8d7r: Cryo-EM structure of human CLCF1 signaling complex: model containing the interaction core region Method: EM (single particle) / Resolution: 3.9 Å 27244 8d82 EMDB-27244: Cryo-EM map of detergent-solubilized human IL-6 signaling complex with full extracellular domains PDB-8d82: Cryo-EM structure of human IL-6 signaling complex in detergent: model containing full extracellular domains Method: EM (single particle) / Resolution: 3.22 Å EMDB-27246: Cryo-EM map of human IL-27 signaling complex with full extracellular domains Method: EM (single particle) / Resolution: 4.14 Å 27247 8d85 EMDB-27247: Cryo-EM map of human IL-27 signaling complex: focused refinement on the interaction core region PDB-8d85: Cryo-EM structure of human IL-27 signaling complex: model containing the interaction core region Method: EM (single particle) / Resolution: 3.81 Å
Chemicals	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine
Source	homo sapiens (human)
Keywords	CYTOKINE / cytokine signaling / LIF / gp130 / LIFR / CNTF / CNTFR alpha / CYTOKINE/Immune System / antibody / Fab / CYTOKINE-Immune System complex / CLCF1 / CRLF1 / IL-6 / IL-6R alpha / IL-27 / IL-27R alpha / EBI3 / p28