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- PDB-8d7h: Cryo-EM structure of human CLCF1 in complex with CRLF1 and CNTFR alpha -

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Basic information

Entry
Database: PDB / ID: 8d7h
TitleCryo-EM structure of human CLCF1 in complex with CRLF1 and CNTFR alpha
Components
  • Cardiotrophin-like cytokine factor 1
  • Ciliary neurotrophic factor receptor subunit alpha
  • Cytokine receptor-like factor 1
KeywordsCYTOKINE / cytokine signaling / CLCF1 / CNTFR alpha / CRLF1
Function / homology
Function and homology information


CRLF-CLCF1 complex / CNTFR-CLCF1 complex / skeletal muscle organ development / positive regulation of isotype switching to IgE isotypes / ciliary neurotrophic factor receptor activity / ciliary neurotrophic factor receptor binding / ciliary neurotrophic factor receptor complex / ciliary neurotrophic factor-mediated signaling pathway / brainstem development / sex differentiation ...CRLF-CLCF1 complex / CNTFR-CLCF1 complex / skeletal muscle organ development / positive regulation of isotype switching to IgE isotypes / ciliary neurotrophic factor receptor activity / ciliary neurotrophic factor receptor binding / ciliary neurotrophic factor receptor complex / ciliary neurotrophic factor-mediated signaling pathway / brainstem development / sex differentiation / positive regulation of astrocyte differentiation / negative regulation of motor neuron apoptotic process / Interleukin-27 signaling / IL-6-type cytokine receptor ligand interactions / cytokine receptor activity / motor neuron apoptotic process / ureteric bud development / extrinsic component of membrane / suckling behavior / cytokine binding / cell surface receptor signaling pathway via JAK-STAT / positive regulation of immunoglobulin production / positive regulation of B cell proliferation / positive regulation of tyrosine phosphorylation of STAT protein / B cell differentiation / cytokine activity / growth factor activity / cytokine-mediated signaling pathway / nervous system development / negative regulation of neuron apoptotic process / cell surface receptor signaling pathway / receptor complex / apical plasma membrane / external side of plasma membrane / signaling receptor binding / positive regulation of cell population proliferation / signal transduction / extracellular space / extracellular region / plasma membrane / cytosol
Similarity search - Function
Plethodontid receptivity factor PRF/cardiotrophin-like / Plethodontid receptivity factor PRF / Growth hormone/erythropoietin receptor, ligand binding / Erythropoietin receptor, ligand binding / Long hematopoietin receptor, soluble alpha chain, conserved site / Long hematopoietin receptor, soluble alpha chains family signature. / Four-helical cytokine-like, core / Fibronectin type III domain / Fibronectin type 3 domain / Immunoglobulin subtype 2 ...Plethodontid receptivity factor PRF/cardiotrophin-like / Plethodontid receptivity factor PRF / Growth hormone/erythropoietin receptor, ligand binding / Erythropoietin receptor, ligand binding / Long hematopoietin receptor, soluble alpha chain, conserved site / Long hematopoietin receptor, soluble alpha chains family signature. / Four-helical cytokine-like, core / Fibronectin type III domain / Fibronectin type 3 domain / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Fibronectin type-III domain profile. / Fibronectin type III / Fibronectin type III superfamily / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
Cytokine receptor-like factor 1 / Ciliary neurotrophic factor receptor subunit alpha / Cardiotrophin-like cytokine factor 1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.4 Å
AuthorsZhou, Y. / Franklin, M.C.
Funding support1items
OrganizationGrant numberCountry
Other private
CitationJournal: Sci Adv / Year: 2023
Title: Structural insights into the assembly of gp130 family cytokine signaling complexes.
Authors: Yi Zhou / Panayiotis E Stevis / Jing Cao / Kei Saotome / Jiaxi Wu / Arielle Glatman Zaretsky / Sokol Haxhinasto / George D Yancopoulos / Andrew J Murphy / Mark W Sleeman / William C Olson / Matthew C Franklin /
Abstract: The interleukin-6 (IL-6) family cytokines signal through gp130 receptor homodimerization or heterodimerization with a second signaling receptor and play crucial roles in various cellular processes. ...The interleukin-6 (IL-6) family cytokines signal through gp130 receptor homodimerization or heterodimerization with a second signaling receptor and play crucial roles in various cellular processes. We determined cryo-electron microscopy structures of five signaling complexes of this family, containing full receptor ectodomains bound to their respective ligands ciliary neurotrophic factor, cardiotrophin-like cytokine factor 1 (CLCF1), leukemia inhibitory factor, IL-27, and IL-6. Our structures collectively reveal similarities and differences in the assembly of these complexes. The acute bends at both signaling receptors in all complexes bring the membrane-proximal domains to a ~30 angstrom range but with distinct distances and orientations. We also reveal how CLCF1 engages its secretion chaperone cytokine receptor-like factor 1. Our data provide valuable insights for therapeutically targeting gp130-mediated signaling.
History
DepositionJun 7, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 29, 2023Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
C: Ciliary neurotrophic factor receptor subunit alpha
D: Cardiotrophin-like cytokine factor 1
A: Cytokine receptor-like factor 1
G: Ciliary neurotrophic factor receptor subunit alpha
H: Cardiotrophin-like cytokine factor 1
E: Cytokine receptor-like factor 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)219,46624
Polymers212,6406
Non-polymers6,82618
Water0
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein Ciliary neurotrophic factor receptor subunit alpha / / CNTF receptor subunit alpha / CNTFR-alpha


Mass: 39170.848 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CNTFR / Production host: Cricetulus griseus (Chinese hamster) / References: UniProt: P26992
#2: Protein Cardiotrophin-like cytokine factor 1 / B-cell-stimulating factor 3 / BSF-3 / Novel neurotrophin-1 / NNT-1


Mass: 23122.336 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CLCF1, BSF3, CLC, NNT1 / Production host: Mus musculus (house mouse) / References: UniProt: Q9UBD9
#3: Protein Cytokine receptor-like factor 1 / Cytokine-like factor 1 / CLF-1 / ZcytoR5


Mass: 44026.652 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CRLF1, UNQ288/PRO327 / Production host: Mus musculus (house mouse) / References: UniProt: O75462
#4: Polysaccharide
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 14
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}}LINUCSPDB-CARE
#5: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE / N-Acetylglucosamine


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Human CLCF1 in complex with in complex with CRLF1 and CNTFR alpha
Type: COMPLEX / Entity ID: #1-#3 / Source: RECOMBINANT
Source (natural)Organism: Homo sapiens (human)
Source (recombinant)Organism: Cricetulus griseus (Chinese hamster)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2600 nm / Nominal defocus min: 1400 nm
Image recordingElectron dose: 40 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

SoftwareName: PHENIX / Version: 1.17.1_3660: / Classification: refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C2 (2 fold cyclic)
3D reconstructionResolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 117773 / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00212918
ELECTRON MICROSCOPYf_angle_d0.43517668
ELECTRON MICROSCOPYf_dihedral_angle_d21.5771858
ELECTRON MICROSCOPYf_chiral_restr0.0392022
ELECTRON MICROSCOPYf_plane_restr0.0042230

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