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Structure paper

TitleRhodobacter capsulatus forms a compact crescent-shaped LH1-RC photocomplex.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 846, Year 2023
Publish dateFeb 15, 2023
AuthorsKazutoshi Tani / Ryo Kanno / Xuan-Cheng Ji / Itsusei Satoh / Yuki Kobayashi / Malgorzata Hall / Long-Jiang Yu / Yukihiro Kimura / Akira Mizoguchi / Bruno M Humbel / Michael T Madigan / Zheng-Yu Wang-Otomo /
PubMed AbstractRhodobacter (Rba.) capsulatus has been a favored model for studies of all aspects of bacterial photosynthesis. This purple phototroph contains PufX, a polypeptide crucial for dimerization of the ...Rhodobacter (Rba.) capsulatus has been a favored model for studies of all aspects of bacterial photosynthesis. This purple phototroph contains PufX, a polypeptide crucial for dimerization of the light-harvesting 1-reaction center (LH1-RC) complex, but lacks protein-U, a U-shaped polypeptide in the LH1-RC of its close relative Rba. sphaeroides. Here we present a cryo-EM structure of the Rba. capsulatus LH1-RC purified by DEAE chromatography. The crescent-shaped LH1-RC exhibits a compact structure containing only 10 LH1 αβ-subunits. Four αβ-subunits corresponding to those adjacent to protein-U in Rba. sphaeroides were absent. PufX in Rba. capsulatus exhibits a unique conformation in its N-terminus that self-associates with amino acids in its own transmembrane domain and interacts with nearby polypeptides, preventing it from interacting with proteins in other complexes and forming dimeric structures. These features are discussed in relation to the minimal requirements for the formation of LH1-RC monomers and dimers, the spectroscopic behavior of both the LH1 and RC, and the bioenergetics of energy transfer from LH1 to the RC.
External linksNat Commun / PubMed:36792596 / PubMed Central
MethodsEM (single particle)
Resolution2.6 Å
Structure data

33931

7yml

EMDB-33931, PDB-7yml:
Structure of photosynthetic LH1-RC super-complex of Rhodobacter capsulatus
Method: EM (single particle) / Resolution: 2.6 Å

Chemicals

ChemComp-BCL:
BACTERIOCHLOROPHYLL A / Bacteriochlorophyll

ChemComp-BPH:
BACTERIOPHEOPHYTIN A / Pheophytin

ChemComp-U10:
UBIQUINONE-10 / Coenzyme Q10

ChemComp-PGV:
(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE / phospholipid*YM / Phosphatidylglycerol

ChemComp-PEE:
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipid*YM / Discrete optimized protein energy

ChemComp-LDA:
LAURYL DIMETHYLAMINE-N-OXIDE / LDAO, detergent*YM / Lauryldimethylamine oxide

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-FE:
Unknown entry / Iron

ChemComp-SPO:
SPHEROIDENE

ChemComp-MYR:
MYRISTIC ACID / Myristic acid

ChemComp-HOH:
WATER / Water

Source
  • rhodobacter capsulatus (bacteria)
KeywordsPHOTOSYNTHESIS / LH1-RC COMPLEX / PURPLE BACTERIA

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