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TitleSubdomains of the Cag T4SS outer membrane core complex exhibit structural independence.
Journal, issue, pagesLife Sci Alliance, Vol. 7, Issue 6, Year 2024
Publish dateApr 17, 2024
AuthorsJacquelyn R Roberts / Sirena C Tran / Arwen E Frick-Cheng / Kaeli N Bryant / Chiamaka D Okoye / W Hayes McDonald / Timothy L Cover / Melanie D Ohi /
PubMed AbstractThe Cag type IV secretion system (Cag T4SS) has an important role in the pathogenesis of gastric cancer. The Cag T4SS outer membrane core complex (OMCC) is organized into three regions: a 14-fold ...The Cag type IV secretion system (Cag T4SS) has an important role in the pathogenesis of gastric cancer. The Cag T4SS outer membrane core complex (OMCC) is organized into three regions: a 14-fold symmetric outer membrane cap (OMC) composed of CagY, CagX, CagT, CagM, and Cag3; a 17-fold symmetric periplasmic ring (PR) composed of CagY and CagX; and a stalk with unknown composition. We investigated how CagT, CagM, and a conserved antenna projection (AP) region of CagY contribute to the structural organization of the OMCC. Single-particle cryo-EM analyses showed that complexes purified from Δ or Δ mutants no longer had organized OMCs, but the PRs remained structured. OMCCs purified from a CagY antenna projection mutant (CagYAP) were structurally similar to WT OMCCs, except for the absence of the α-helical antenna projection. These results indicate that CagY and CagX are sufficient for maintaining a stable PR, but the organization of the OMC requires CagY, CagX, CagM, and CagT. Our results highlight an unexpected structural independence of two major subdomains of the Cag T4SS OMCC.
External linksLife Sci Alliance / PubMed:38631913 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 8.5 Å
Structure data

EMDB-42290: Cryo-EM Structure of the Helicobacter pylori CagYdAP OMC
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-42393: Cryo-EM Structure of the Helicobacter pylori dcagM PR
Method: EM (single particle) / Resolution: 8.5 Å

EMDB-44587: Cryo-EM Structure of the Helicobacter pylori dcagT PR
Method: EM (single particle) / Resolution: 8.0 Å

Source
  • Helicobacter pylori 26695 (bacteria)

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