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TitleStructure of the R2 non-LTR retrotransposon initiating target-primed reverse transcription.
Journal, issue, pagesScience, Vol. 380, Issue 6642, Page 301-308, Year 2023
Publish dateApr 21, 2023
AuthorsMax E Wilkinson / Chris J Frangieh / Rhiannon K Macrae / Feng Zhang /
PubMed AbstractNon-long terminal repeat (non-LTR) retrotransposons, or long interspersed nuclear elements (LINEs), are an abundant class of eukaryotic transposons that insert into genomes by target-primed reverse ...Non-long terminal repeat (non-LTR) retrotransposons, or long interspersed nuclear elements (LINEs), are an abundant class of eukaryotic transposons that insert into genomes by target-primed reverse transcription (TPRT). During TPRT, a target DNA sequence is nicked and primes reverse transcription of the retrotransposon RNA. Here, we report the cryo-electron microscopy structure of the R2 non-LTR retrotransposon initiating TPRT at its ribosomal DNA target. The target DNA sequence is unwound at the insertion site and recognized by an upstream motif. An extension of the reverse transcriptase (RT) domain recognizes the retrotransposon RNA and guides the 3' end into the RT active site to template reverse transcription. We used Cas9 to retarget R2 in vitro to non-native sequences, suggesting future use as a reprogrammable RNA-based gene-insertion tool.
External linksScience / PubMed:37023171 / PubMed Central
MethodsEM (single particle)
Resolution3.08 Å
Structure data

40033

8gh6

EMDB-40033, PDB-8gh6:
Bombyx mori R2 retrotransposon initiating target-primed reverse transcription
Method: EM (single particle) / Resolution: 3.08 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-TTP:
THYMIDINE-5'-TRIPHOSPHATE / Thymidine triphosphate

Source
  • bombyx mori (domestic silkworm)
KeywordsRNA BINDING PROTEIN/RNA/DNA / retrotransposon / LINE / reverse transcriptase / endonuclease / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA-DNA complex

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