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-Structure paper
タイトル | Structure of the R2 non-LTR retrotransposon initiating target-primed reverse transcription. |
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ジャーナル・号・ページ | Science, Vol. 380, Issue 6642, Page 301-308, Year 2023 |
掲載日 | 2023年4月21日 |
著者 | Max E Wilkinson / Chris J Frangieh / Rhiannon K Macrae / Feng Zhang / |
PubMed 要旨 | Non-long terminal repeat (non-LTR) retrotransposons, or long interspersed nuclear elements (LINEs), are an abundant class of eukaryotic transposons that insert into genomes by target-primed reverse ...Non-long terminal repeat (non-LTR) retrotransposons, or long interspersed nuclear elements (LINEs), are an abundant class of eukaryotic transposons that insert into genomes by target-primed reverse transcription (TPRT). During TPRT, a target DNA sequence is nicked and primes reverse transcription of the retrotransposon RNA. Here, we report the cryo-electron microscopy structure of the R2 non-LTR retrotransposon initiating TPRT at its ribosomal DNA target. The target DNA sequence is unwound at the insertion site and recognized by an upstream motif. An extension of the reverse transcriptase (RT) domain recognizes the retrotransposon RNA and guides the 3' end into the RT active site to template reverse transcription. We used Cas9 to retarget R2 in vitro to non-native sequences, suggesting future use as a reprogrammable RNA-based gene-insertion tool. |
リンク | Science / PubMed:37023171 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.08 Å |
構造データ | EMDB-40033, PDB-8gh6: |
化合物 | ChemComp-ZN: ChemComp-MG: ChemComp-TTP: |
由来 |
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キーワード | RNA BINDING PROTEIN/RNA/DNA / retrotransposon (レトロトランスポゾン) / LINE (ライン) / reverse transcriptase (逆転写酵素) / endonuclease (エンドヌクレアーゼ) / RNA BINDING PROTEIN (RNA結合タンパク質) / RNA BINDING PROTEIN-RNA-DNA complex |