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TitleCryo-EM structure of SARS-CoV-2 postfusion spike in membrane.
Journal, issue, pagesNature, Vol. 619, Issue 7969, Page 403-409, Year 2023
Publish dateJun 7, 2023
AuthorsWei Shi / Yongfei Cai / Haisun Zhu / Hanqin Peng / Jewel Voyer / Sophia Rits-Volloch / Hong Cao / Megan L Mayer / Kangkang Song / Chen Xu / Jianming Lu / Jun Zhang / Bing Chen /
PubMed AbstractThe entry of SARS-CoV-2 into host cells depends on the refolding of the virus-encoded spike protein from a prefusion conformation, which is metastable after cleavage, to a lower-energy stable ...The entry of SARS-CoV-2 into host cells depends on the refolding of the virus-encoded spike protein from a prefusion conformation, which is metastable after cleavage, to a lower-energy stable postfusion conformation. This transition overcomes kinetic barriers for fusion of viral and target cell membranes. Here we report a cryogenic electron microscopy (cryo-EM) structure of the intact postfusion spike in a lipid bilayer that represents the single-membrane product of the fusion reaction. The structure provides structural definition of the functionally critical membrane-interacting segments, including the fusion peptide and transmembrane anchor. The internal fusion peptide forms a hairpin-like wedge that spans almost the entire lipid bilayer and the transmembrane segment wraps around the fusion peptide at the last stage of membrane fusion. These results advance our understanding of the spike protein in a membrane environment and may guide development of intervention strategies.
External linksNature / PubMed:37285872
MethodsEM (single particle)
Resolution2.9 - 3.4 Å
Structure data

29016

8fdw

EMDB-29016, PDB-8fdw:
Cryo-EM structure of SARS-CoV-2 postfusion spike in membrane
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-29017: Cryo-EM structure of SARS-CoV-2 postfusion spike in membrane
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-29018: Cryo-EM structure of SARS-CoV-2 postfusion spike in membrane
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-MAN:
alpha-D-mannopyranose / Mannose

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

Source
  • Severe acute respiratory syndrome coronavirus 2
  • severe acute respiratory syndrome coronavirus
KeywordsVIRAL PROTEIN

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