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TitleStructural insights into the Venus flytrap mechanosensitive ion channel Flycatcher1.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 850, Year 2022
Publish dateFeb 14, 2022
AuthorsSebastian Jojoa-Cruz / Kei Saotome / Che Chun Alex Tsui / Wen-Hsin Lee / Mark S P Sansom / Swetha E Murthy / Ardem Patapoutian / Andrew B Ward /
PubMed AbstractFlycatcher1 (FLYC1), a MscS homolog, has recently been identified as a candidate mechanosensitive (MS) ion channel involved in Venus flytrap prey recognition. FLYC1 is a larger protein and its ...Flycatcher1 (FLYC1), a MscS homolog, has recently been identified as a candidate mechanosensitive (MS) ion channel involved in Venus flytrap prey recognition. FLYC1 is a larger protein and its sequence diverges from previously studied MscS homologs, suggesting it has unique structural features that contribute to its function. Here, we characterize FLYC1 by cryo-electron microscopy, molecular dynamics simulations, and electrophysiology. Akin to bacterial MscS and plant MSL1 channels, we find that FLYC1 central core includes side portals in the cytoplasmic cage that regulate ion preference and conduction, by identifying critical residues that modulate channel conductance. Topologically unique cytoplasmic flanking regions can adopt 'up' or 'down' conformations, making the channel asymmetric. Disruption of an up conformation-specific interaction severely delays channel deactivation by 40-fold likely due to stabilization of the channel open state. Our results illustrate novel structural features and likely conformational transitions that regulate mechano-gating of FLYC1.
External linksNat Commun / PubMed:35165281 / PubMed Central
MethodsEM (single particle)
Resolution2.4 - 2.8 Å
Structure data

24186

7n5d

EMDB-24186, PDB-7n5d:
Composite Structure of Mechanosensitive Ion Channel Flycatcher1 in GDN
Method: EM (single particle) / Resolution: 2.8 Å

24187

7n5e

EMDB-24187, PDB-7n5e:
Structure of Mechanosensitive Ion Channel Flycatcher1 in GDN
Method: EM (single particle) / Resolution: 2.8 Å

24188

7n5f

EMDB-24188, PDB-7n5f:
Structure of Mechanosensitive Ion Channel Flycatcher1 Protomer in 'Down' conformation in GDN
Method: EM (single particle) / Resolution: 2.7 Å

24189

7n5g

EMDB-24189, PDB-7n5g:
Structure of Mechanosensitive Ion Channel Flycatcher1 Protomer in 'Up' conformation in GDN
Method: EM (single particle) / Resolution: 2.4 Å

Chemicals

ChemComp-PLM:
PALMITIC ACID / Palmitic acid

Source
  • dionaea muscipula (Venus flytrap)
KeywordsMEMBRANE PROTEIN / mechanically activated ion channel

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