EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Dynamics of the Herpes simplex virus DNA polymerase holoenzyme during DNA synthesis and proof-reading revealed by Cryo-EM.
ジャーナル・号・ページ	Nucleic Acids Res, Year 2024
掲載日	2024年5月29日
著者	Emil Gustavsson / Kay Grünewald / Per Elias / B Martin Hällberg /
PubMed 要旨	Herpes simplex virus 1 (HSV-1), a double-stranded DNA virus, replicates using seven essential proteins encoded by its genome. Among these, the UL30 DNA polymerase, complexed with the UL42 ...Herpes simplex virus 1 (HSV-1), a double-stranded DNA virus, replicates using seven essential proteins encoded by its genome. Among these, the UL30 DNA polymerase, complexed with the UL42 processivity factor, orchestrates leading and lagging strand replication of the 152 kb viral genome. UL30 polymerase is a prime target for antiviral therapy, and resistance to current drugs can arise in immunocompromised individuals. Using electron cryo-microscopy (cryo-EM), we unveil the dynamic changes of the UL30/UL42 complex with DNA in three distinct states. First, a pre-translocation state with an open fingers domain ready for nucleotide incorporation. Second, a halted elongation state where the fingers close, trapping dATP in the dNTP pocket. Third, a DNA-editing state involving significant conformational changes to allow DNA realignment for exonuclease activity. Additionally, the flexible UL30 C-terminal domain interacts with UL42, forming an extended positively charged surface binding to DNA, thereby enhancing processive synthesis. These findings highlight substantial structural shifts in the polymerase and its DNA interactions during replication, offering insights for future antiviral drug development.
リンク	Nucleic Acids Res / PubMed:38806233
手法	EM (単粒子)
解像度	1.87 - 3.11 Å
構造データ	16906 8oj6 EMDB-16906, PDB-8oj6: HSV-1 DNA polymerase-processivity factor complex in pre-translocation state 手法: EM (単粒子) / 解像度: 2.41 Å 16907 8oj7 EMDB-16907, PDB-8oj7: HSV-1 DNA polymerase-processivity factor complex in halted elongation state 手法: EM (単粒子) / 解像度: 2.46 Å 16909 8oja EMDB-16909, PDB-8oja: HSV-1 DNA polymerase-processivity factor complex in exonuclease state 手法: EM (単粒子) / 解像度: 1.87 Å 16910 8ojb EMDB-16910, PDB-8ojb: HSV-1 DNA polymerase-processivity factor complex in exonuclease state active site 手法: EM (単粒子) / 解像度: 1.9 Å 16911 8ojc EMDB-16911, PDB-8ojc: HSV-1 DNA polymerase active site in alternative exonuclease state 手法: EM (単粒子) / 解像度: 2.08 Å 16912 8ojd EMDB-16912, PDB-8ojd: HSV-1 DNA polymerase beta-hairpin loop 手法: EM (単粒子) / 解像度: 2.46 Å EMDB-16918: Focused refinement map of HSV-1 DNA polymerase in pre-translocation state 手法: EM (単粒子) / 解像度: 2.4 Å EMDB-16919: Focused refinement map of HSV-1 DNA polymerase processivity factor in pre-translocation state 手法: EM (単粒子) / 解像度: 3.11 Å EMDB-16924: Focused refinement of HSV-1 DNA polymerase in halted elongation state 手法: EM (単粒子) / 解像度: 2.42 Å EMDB-16925: Focused refinement of HSV-1 DNA polymerase processivity factor in halted elongation state 手法: EM (単粒子) / 解像度: 2.97 Å EMDB-16927: Focused refinement map of HSV-1 DNA polymerase in exonuclease state 手法: EM (単粒子) / 解像度: 1.89 Å EMDB-16928: Focused refinement map of HSV-1 DNA polymerase processivity factor in exonuclease state 手法: EM (単粒子) / 解像度: 2.35 Å EMDB-17013: HSV-1 DNA polymerase-processivity factor complex in halted elongation state consensus map 手法: EM (単粒子) / 解像度: 2.4 Å EMDB-17014: Consensus map of HSV-1 DNA polymerase-processivity factor complex in pre-translocation state 手法: EM (単粒子) / 解像度: 2.41 Å EMDB-17018: Consensus map of HSV-1 DNA polymerase-processivity factor complex in exonuclease state 手法: EM (単粒子) / 解像度: 2.03 Å 19837 9enp EMDB-19837, PDB-9enp: HSV-1 DNA polymerase-processivity factor complex in exonuclease state with 1-bp DNA mismatch 手法: EM (単粒子) / 解像度: 2.12 Å 19838 9enq EMDB-19838, PDB-9enq: HSV-1 DNA polymerase-processivity factor complex in exonuclease state active site with 1-bp DNA mismatch 手法: EM (単粒子) / 解像度: 2.12 Å EMDB-19839: HSV-1 DNA polymerase-processivity factor complex in exonuclease state with 1-bp DNA mismatch consensus map 手法: EM (単粒子) / 解像度: 2.23 Å EMDB-19840: HSV-1 DNA polymerase-processivity factor complex in exonuclease state with 1-bp DNA mismatch catalytic core focused map 手法: EM (単粒子) / 解像度: 2.1 Å EMDB-19841: HSV-1 DNA polymerase-processivity factor complex in exonuclease state with 1-bp DNA mismatch processivity factor focused refinement 手法: EM (単粒子) / 解像度: 2.87 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER / 水 ChemComp-DTP: 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE / dATP / デオキシアデノシン三リン酸 ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	human alphaherpesvirus 1 strain kos (ヘルペスウイルス) synthetic construct (人工物)
キーワード	TRANSFERASE (転移酵素) / DNA (デオキシリボ核酸) / Polymerase (ポリメラーゼ) / Complex