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| Title | Molecular mechanism of Oxr1p mediated disassembly of yeast V-ATPase. |
|---|---|
| Journal, issue, pages | EMBO Rep, Year 2024 |
| Publish date | Apr 2, 2024 |
 Authors | Md Murad Khan / Stephan Wilkens /  |
| PubMed Abstract | The eukaryotic vacuolar H-ATPase (V-ATPase) is regulated by reversible disassembly into autoinhibited V-ATPase and V proton channel subcomplexes. We recently reported that the TLDc protein Oxr1p ...The eukaryotic vacuolar H-ATPase (V-ATPase) is regulated by reversible disassembly into autoinhibited V-ATPase and V proton channel subcomplexes. We recently reported that the TLDc protein Oxr1p induces V-ATPase disassembly in vitro. Whether and how Oxr1p is involved in enzyme disassembly in vivo, however, is not known. Here, using yeast genetics and fluorescence microscopy, we show that Oxr1p is essential for efficient V-ATPase disassembly in the cell. Supporting biochemical and biophysical in vitro experiments show that whereas Oxr1p-driven holoenzyme disassembly can occur in the absence of nucleotides, the presence of ATP greatly accelerates the process. ATP hydrolysis is needed, however, for subsequent release of Oxr1p so that the free V can adopt the autoinhibited conformation. Overall, our study unravels the molecular mechanism of Oxr1p-induced disassembly that occurs in vivo as part of the canonical V-ATPase regulation by reversible disassembly. |
 External links | EMBO Rep / PubMed:38565737 |
| Methods | EM (single particle) |
| Resolution | 8.1 Å |
| Structure data |  EMDB-42880: State 1 Yeast V-ATPase with Oxr1p bound  EMDB-42881: State 2 Yeast V-ATPase without Oxr1p bound |
| Source |
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Saccharomyces cerevisiae (brewer's yeast)