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Structure paper

TitleFilament structure of bacterial tubulin homologue TubZ.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 107, Issue 46, Page 19766-19771, Year 2010
Publish dateNov 16, 2010
AuthorsChristopher H S Aylett / Qing Wang / Katharine A Michie / Linda A Amos / Jan Löwe /
PubMed AbstractLow copy number plasmids often depend on accurate partitioning systems for their continued survival. Generally, such systems consist of a centromere-like region of DNA, a DNA-binding adaptor, and a ...Low copy number plasmids often depend on accurate partitioning systems for their continued survival. Generally, such systems consist of a centromere-like region of DNA, a DNA-binding adaptor, and a polymerizing cytomotive filament. Together these components drive newly replicated plasmids to opposite ends of the dividing cell. The Bacillus thuringiensis plasmid pBToxis relies on a filament of the tubulin/FtsZ-like protein TubZ for its segregation. By combining crystallography and electron microscopy, we have determined the structure of this filament. We explain how GTP hydrolysis weakens the subunit-subunit contact and also shed light on the partitioning of the plasmid-adaptor complex. The double helical superstructure of TubZ filaments is unusual for tubulin-like proteins. Filaments of ParM, the actin-like partitioning protein, are also double helical. We suggest that convergent evolution shapes these different types of cytomotive filaments toward a general mechanism for plasmid separation.
External linksProc Natl Acad Sci U S A / PubMed:20974911 / PubMed Central
MethodsEM (helical sym.) / X-ray diffraction
Resolution3 - 35.0 Å
Structure data

EMDB-1757:
The Structure of TubZ filaments
Method: EM (helical sym.) / Resolution: 35.0 Å

EMDB-1758:
Quadruple filaments of TubZ
Method: EM (helical sym.) / Resolution: 35.0 Å

EMDB-1759:
Double helical filaments of an N-terminal thioredoxin fusion of TubZ
Method: EM (helical sym.) / Resolution: 35.0 Å

EMDB-1760:
Double filaments of C-terminally GFP-fused TubZ
Method: EM (helical sym.) / Resolution: 35.0 Å

PDB-2xka:
Crystal structure of a GTPyS-form protofilament of Bacillus thuringiensis serovar israelensis TubZ
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-2xkb:
Crystal structure of GDP-form protofilaments of Bacillus thuringiensis serovar israelensis TubZ
Method: X-RAY DIFFRACTION / Resolution: 3 Å

Chemicals

ChemComp-GSP:
5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE

ChemComp-MG:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

Source
  • bacillus thuringiensis (bacteria)
KeywordsSTRUCTURAL PROTEIN / MOTOR PROTEIN / CYTOSKELETON / CYTOMOTIVE / DNA SEGREGATION / MICROTUBULE / PBTOXIS / PBT156 / REPX / TUBR

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