EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Filament structure of bacterial tubulin homologue TubZ.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 107, Issue 46, Page 19766-19771, Year 2010
掲載日	2010年11月16日
著者	Christopher H S Aylett / Qing Wang / Katharine A Michie / Linda A Amos / Jan Löwe /
PubMed 要旨	Low copy number plasmids often depend on accurate partitioning systems for their continued survival. Generally, such systems consist of a centromere-like region of DNA, a DNA-binding adaptor, and a ...Low copy number plasmids often depend on accurate partitioning systems for their continued survival. Generally, such systems consist of a centromere-like region of DNA, a DNA-binding adaptor, and a polymerizing cytomotive filament. Together these components drive newly replicated plasmids to opposite ends of the dividing cell. The Bacillus thuringiensis plasmid pBToxis relies on a filament of the tubulin/FtsZ-like protein TubZ for its segregation. By combining crystallography and electron microscopy, we have determined the structure of this filament. We explain how GTP hydrolysis weakens the subunit-subunit contact and also shed light on the partitioning of the plasmid-adaptor complex. The double helical superstructure of TubZ filaments is unusual for tubulin-like proteins. Filaments of ParM, the actin-like partitioning protein, are also double helical. We suggest that convergent evolution shapes these different types of cytomotive filaments toward a general mechanism for plasmid separation.
リンク	Proc Natl Acad Sci U S A / PubMed:20974911 / PubMed Central
手法	EM (らせん対称) / X線回折
解像度	3 - 35.0 Å
構造データ	EMDB-1757: The Structure of TubZ filaments 手法: EM (らせん対称) / 解像度: 35.0 Å EMDB-1758: Quadruple filaments of TubZ 手法: EM (らせん対称) / 解像度: 35.0 Å EMDB-1759: Double helical filaments of an N-terminal thioredoxin fusion of TubZ 手法: EM (らせん対称) / 解像度: 35.0 Å EMDB-1760: Double filaments of C-terminally GFP-fused TubZ 手法: EM (らせん対称) / 解像度: 35.0 Å PDB-2xka: Crystal structure of a GTPyS-form protofilament of Bacillus thuringiensis serovar israelensis TubZ 手法: X-RAY DIFFRACTION / 解像度: 3.0 Å PDB-2xkb: Crystal structure of GDP-form protofilaments of Bacillus thuringiensis serovar israelensis TubZ 手法: X-RAY DIFFRACTION / 解像度: 3.0 Å
化合物	ChemComp-GSP: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE / GTP-γ-S ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子*YM / グアノシン二リン酸
由来	bacillus thuringiensis (バチルス・チューリンゲンシス)
キーワード	STRUCTURAL PROTEIN (タンパク質) / MOTOR PROTEIN (モータータンパク質) / CYTOSKELETON (細胞骨格) / CYTOMOTIVE / DNA SEGREGATION / MICROTUBULE (微小管) / PBTOXIS / PBT156 / REPX / TUBR (サナア県)