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TitleStructural basis of DNA crossover capture by DNA gyrase.
Journal, issue, pagesScience, Vol. 384, Issue 6692, Page 227-232, Year 2024
Publish dateApr 12, 2024
AuthorsMarlène Vayssières / Nils Marechal / Long Yun / Brian Lopez Duran / Naveen Kumar Murugasamy / Jonathan M Fogg / Lynn Zechiedrich / Marc Nadal / Valérie Lamour /
PubMed AbstractDNA supercoiling must be precisely regulated by topoisomerases to prevent DNA entanglement. The interaction of type IIA DNA topoisomerases with two DNA molecules, enabling the transport of one duplex ...DNA supercoiling must be precisely regulated by topoisomerases to prevent DNA entanglement. The interaction of type IIA DNA topoisomerases with two DNA molecules, enabling the transport of one duplex through the transient double-stranded break of the other, remains elusive owing to structures derived solely from single linear duplex DNAs lacking topological constraints. Using cryo-electron microscopy, we solved the structure of DNA gyrase bound to a negatively supercoiled minicircle DNA. We show how DNA gyrase captures a DNA crossover, revealing both conserved molecular grooves that accommodate the DNA helices. Together with molecular tweezer experiments, the structure shows that the DNA crossover is of positive chirality, reconciling the binding step of gyrase-mediated DNA relaxation and supercoiling in a single structure.
External linksScience / PubMed:38603484
MethodsEM (single particle)
Resolution2.9 - 4.2 Å
Structure data

18342

8qdx

EMDB-18342, PDB-8qdx:
E. coli DNA gyrase bound to a DNA crossover
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-18565: E. coli DNA gyrase bound to a DNA crossover
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-18566: Focused map of GyrA-CTD and T-segment DNA from the DNA crossover-gyrase complex
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-18567: Focused map of GyrA-CTD from DNA crossover-gyrase complex
Method: EM (single particle) / Resolution: 4.0 Å

18603

8qqs

EMDB-18603, PDB-8qqs:
E. coli DNA gyrase bound to a linear part of a DNA minicircle
Method: EM (single particle) / Resolution: 2.9 Å

18605

8qqu

EMDB-18605, PDB-8qqu:
Asymetric subunit of E. coli DNA gyrase bound to a linear part of a DNA minicircle
Method: EM (single particle) / Resolution: 2.9 Å

Source
  • escherichia coli (E. coli)
  • synthetic construct (others)
KeywordsISOMERASE / DNA-protein complex / type IIA topoisomerase DNA gyrase / type IIA topoisomerase / DNA gyrase

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