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Yorodumi- PDB-9ewq: Influenza virus neuraminidase N1 NC13 ectodomain with a tetrabrac... -
+Open data
-Basic information
Entry | Database: PDB / ID: 9ewq | ||||||
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Title | Influenza virus neuraminidase N1 NC13 ectodomain with a tetrabrachio-domain stalk | ||||||
Components | Neuraminidase | ||||||
Keywords | VIRAL PROTEIN / Neuraminidase / Tetrabrachion | ||||||
Function / homology | Function and homology information exo-alpha-(2->3)-sialidase activity / exo-alpha-(2->6)-sialidase activity / exo-alpha-(2->8)-sialidase activity / exo-alpha-sialidase / viral budding from plasma membrane / carbohydrate metabolic process / host cell plasma membrane / virion membrane / membrane / metal ion binding Similarity search - Function | ||||||
Biological species | Influenza A virus | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3 Å | ||||||
Authors | Roelofs, M.C. / Zeev-Ben-Mordehai, T. | ||||||
Funding support | 1items
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Citation | Journal: To be published Title: Nanoparticle display of neuraminidase elicits enhanced antibody responses and protection against influenza A virus challenge in mice Authors: Pascha, M.N. / Ballegeer, M. / Roelofs, M.C. / Meuris, L. / Albulescu, I.C. / van Kuppeveld, F.J.M. / Hurdiss, D.L. / Bosch, B.J. / Zeev-Ben-Mordehai, T. / Saelens, X. / de Haan, C.A.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 9ewq.cif.gz | 255.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb9ewq.ent.gz | 201.6 KB | Display | PDB format |
PDBx/mmJSON format | 9ewq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ew/9ewq ftp://data.pdbj.org/pub/pdb/validation_reports/ew/9ewq | HTTPS FTP |
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-Related structure data
Related structure data | 50022MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 42385.258 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus / Gene: NA / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: A0A2R4G842, exo-alpha-sialidase |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Influenza N1 NC13 with part of stalk replaced by tetrabrachion tetramerization domain Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: Influenza A virus (A/North Carolina/07/2013(H1N1)) |
Source (recombinant) | Organism: Homo sapiens (human) |
Details of virus | Empty: YES / Enveloped: NO / Isolate: OTHER / Type: VIRUS-LIKE PARTICLE |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE-PROPANE |
-Electron microscopy imaging
Experimental equipment | Model: Talos Arctica / Image courtesy: FEI Company |
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Microscopy | Model: FEI TALOS ARCTICA |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 3000 nm / Nominal defocus min: 1000 nm / Cs: 2.7 mm |
Image recording | Average exposure time: 0.2 sec. / Electron dose: 48.1 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3D reconstruction | Resolution: 3 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 199724 / Symmetry type: POINT |