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Yorodumi- PDB-5csc: STRUCTURE OF AN OPEN FORM OF CHICKEN HEART CITRATE SYNTHASE AT 2.... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5csc | ||||||
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Title | STRUCTURE OF AN OPEN FORM OF CHICKEN HEART CITRATE SYNTHASE AT 2.8 ANGSTROMS RESOLUTION | ||||||
Components | (CITRATE SYNTHASE) x 2 | ||||||
Keywords | OXO-ACID-LYASE | ||||||
Function / homology | Function and homology information citrate (Si)-synthase / The tricarboxylic acid cycle / citrate synthase activity / citrate (Si)-synthase activity / citrate metabolic process / tricarboxylic acid cycle / carbohydrate metabolic process / mitochondrial matrix / mitochondrion Similarity search - Function | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.8 Å | ||||||
Authors | Liao, D.-I. / Karpusas, M. / Remington, S.J. | ||||||
Citation | Journal: Biochemistry / Year: 1991 Title: Crystal structure of an open conformation of citrate synthase from chicken heart at 2.8-A resolution Authors: Liao, D.-I. / Karpusas, M. / Remington, S.J. #1: Journal: J.Mol.Biol. / Year: 1984 Title: Crystal Structure Analysis and Molecular Model of a Complex of Citrate Synthase with Oxaloacetate and S-Acetonyl-Coenzymea Authors: Wiegand, G. / Remington, S. / Deisenhofer, J. / Huber, R. #2: Journal: J.Mol.Biol. / Year: 1982 Title: Crystallographic Refinement and Atomic Models of Two Different Forms of Citrate Synthase at 2.7 And 1.7 Angstroms Resolution Authors: Remington, S. / Wiegand, G. / Huber, R. #3: Journal: Proc.Natl.Acad.Sci.USA / Year: 1981 Title: Primary Structure of Porcine Heart Citrate Synthase Authors: Bloxham, D.P. / Parmelee, D.C. / Kumar, S. / Wade, R.D. / Ericsson, L.H. / Neurath, H. / Walsh, K.A. / Titani, K. #4: Journal: Eur.J.Biochem. / Year: 1979 Title: Crystal Structure Analysis of the Tetragonal Crystal Form and Preliminary Molecular Model of Pig-Heart Citrate Synthase Authors: Wiegand, G. / Kukla, D. / Scholze, H. / Jones, T.A. / Huber, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5csc.cif.gz | 157.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5csc.ent.gz | 125.3 KB | Display | PDB format |
PDBx/mmJSON format | 5csc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cs/5csc ftp://data.pdbj.org/pub/pdb/validation_reports/cs/5csc | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: AN OCCUPANCY OF 0.0 INDICATES THAT THE ATOM COULD NOT BE FOUND IN THE ELECTRON DENSITY MAP AND IS INCLUDED AS A DUMMY ATOM. | ||||||||
Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.00998, -0.99994, -0.00433), Vector: Details | THE MOLECULE IS A DIMER, WITH ONE DIMER OF MOLECULAR WEIGHT 100000 IN THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT. | |
-Components
#1: Protein | Mass: 47322.172 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Gallus gallus (chicken) / References: UniProt: P23007, EC: 4.1.3.7 |
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#2: Protein | Mass: 46981.750 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Gallus gallus (chicken) / References: UniProt: P23007 |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.38 Å3/Da / Density % sol: 48.29 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 6 / Method: vapor diffusion, hanging drop / Details: or 4 degrees centigrade | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2.7 Å / Num. obs: 15130 / % possible obs: 62.4 % / Num. measured all: 53801 / Rmerge(I) obs: 0.139 |
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Reflection shell | *PLUS Highest resolution: 2.7 Å / Lowest resolution: 2.8 Å / % possible obs: 22 % |
-Processing
Software | Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||
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Refinement | Resolution: 2.8→6 Å / Rfactor obs: 0.197 Details: THE MODEL WAS FIT USING LIMITED RESOLUTION DATA (2.8 ANGSTROMS) AND THERE IS UNCERTAINTY IN THE SEQUENCE USED IN THIS ANALYSIS (SEE REMARK 4). NOTE, AS A CONSEQUENCE, THAT THERE ARE ...Details: THE MODEL WAS FIT USING LIMITED RESOLUTION DATA (2.8 ANGSTROMS) AND THERE IS UNCERTAINTY IN THE SEQUENCE USED IN THIS ANALYSIS (SEE REMARK 4). NOTE, AS A CONSEQUENCE, THAT THERE ARE DISTANCES, TORSION ANGLES, AND BOND ANGLES IN THIS ENTRY WHICH HAVE VALUES OUTSIDE EXPECTED RANGES. | ||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→6 Å
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Refine LS restraints |
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