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- PDB-1d4x: Crystal Structure of Caenorhabditis Elegans Mg-ATP Actin Complexe... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1d4x | ||||||
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Title | Crystal Structure of Caenorhabditis Elegans Mg-ATP Actin Complexed with Human Gelsolin Segment 1 at 1.75 A resolution. | ||||||
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Function / homology | ![]() striated muscle atrophy / regulation of establishment of T cell polarity / regulation of plasma membrane raft polarization / regulation of receptor clustering / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Vorobiev, S. / Ono, S. / Almo, S.C. | ||||||
![]() | ![]() Title: The structure of nonvertebrate actin: implications for the ATP hydrolytic mechanism. Authors: Vorobiev, S. / Strokopytov, B. / Drubin, D.G. / Frieden, C. / Ono, S. / Condeelis, J. / Rubenstein, P.A. / Almo, S.C. #1: ![]() Title: Structure of gelsolin segment 1-actin complex and mechanosm of filament severing Authors: McLaughlin, P.J. / Gooch, J.T. / Mannherz, H.-G. / Weeds, A.G. #2: ![]() Title: Atomic structure of the actin:DNase I complex Authors: Kabsch, W. / Mannherz, H.-G. / Suck, D. / Pai, E.F. / Holmes, K.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 123.7 KB | Display | ![]() |
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PDB format | ![]() | 93.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein , 2 types, 2 molecules AG
#1: Protein | Mass: 41710.492 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Protein | ![]() Mass: 14201.010 Da / Num. of mol.: 1 / Fragment: SEGMENT 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
-Non-polymers , 6 types, 507 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/ATP.gif)
![](data/chem/img/SO2.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/ATP.gif)
![](data/chem/img/SO2.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-MG / |
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#4: Chemical | ChemComp-SO4 / ![]() |
#5: Chemical | ChemComp-ATP / ![]() |
#6: Chemical | ChemComp-SO2 / ![]() |
#7: Chemical | ChemComp-CA / |
#8: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.01 Å3/Da / Density % sol: 59.14 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 100 MM TRIS-HCL, PH 8.0, 2 MM MG-ATP, 1.60 M LI2SO4,, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jan 1, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.75→25 Å / Num. all: 64713 / Num. obs: 64713 / % possible obs: 96.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.91 % / Biso Wilson estimate: 17.041 Å2 / Rmerge(I) obs: 0.041 / Net I/σ(I): 24.2 |
Reflection shell | Resolution: 1.75→1.81 Å / Redundancy: 2.57 % / Rmerge(I) obs: 0.102 / % possible all: 88.3 |
Reflection shell | *PLUS % possible obs: 88.3 % |
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Processing
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Refinement | Resolution: 1.75→23.2 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: R. A. ENGH AND R. HUBER
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Refinement step | Cycle: LAST / Resolution: 1.75→23.2 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 25 Å / Rfactor Rwork![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 24.14 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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