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Yorodumi- PDB-1ap8: TRANSLATION INITIATION FACTOR EIF4E IN COMPLEX WITH M7GDP, NMR, 2... -
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-Basic information
Entry | Database: PDB / ID: 1ap8 | |||||||||
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Title | TRANSLATION INITIATION FACTOR EIF4E IN COMPLEX WITH M7GDP, NMR, 20 STRUCTURES | |||||||||
Components | TRANSLATION INITIATION FACTOR EIF4E | |||||||||
Keywords | RNA CAP / TRANSLATION INITIATION FACTOR | |||||||||
Function / homology | Function and homology information Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / positive regulation of formation of translation preinitiation complex / eukaryotic translation initiation factor 4F complex / mRNA cap binding / RNA 7-methylguanosine cap binding / phosphatidylinositol-3-phosphate binding / regulation of translational initiation / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / Translation initiation complex formation / Ribosomal scanning and start codon recognition ...Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / positive regulation of formation of translation preinitiation complex / eukaryotic translation initiation factor 4F complex / mRNA cap binding / RNA 7-methylguanosine cap binding / phosphatidylinositol-3-phosphate binding / regulation of translational initiation / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / Translation initiation complex formation / Ribosomal scanning and start codon recognition / L13a-mediated translational silencing of Ceruloplasmin expression / translational initiation / translation initiation factor activity / cytoplasmic stress granule / ribosome / regulation of cell cycle / nucleus / cytoplasm Similarity search - Function | |||||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | |||||||||
Method | SOLUTION NMR / molecular dynamics | |||||||||
Authors | Matsuo, H. / Li, H. / Mcguire, A.M. / Fletcher, M. / Gingras, A.C. / Sonenberg, N. / Wagner, G. | |||||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1997 Title: Structure of translation factor eIF4E bound to m7GDP and interaction with 4E-binding protein. Authors: Matsuo, H. / Li, H. / McGuire, A.M. / Fletcher, C.M. / Gingras, A.C. / Sonenberg, N. / Wagner, G. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ap8.cif.gz | 1.3 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1ap8.ent.gz | 1.1 MB | Display | PDB format |
PDBx/mmJSON format | 1ap8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ap/1ap8 ftp://data.pdbj.org/pub/pdb/validation_reports/ap/1ap8 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 24287.203 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Cell line: BL21 (DE3) / Plasmid: BL21 / Cell line (production host): BL21 (DE3) / Production host: Escherichia coli (E. coli) / References: UniProt: P07260 |
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#2: Chemical | ChemComp-M7G / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | pH: 6.5 / Temperature: 303 K |
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-NMR measurement
NMR spectrometer |
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-Processing
Software |
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NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 1 | ||||||||||||
NMR ensemble | Conformer selection criteria: NO VIOLATION ABOVE 0.5 ANGSTROMS AND 5 DEGREE DIHEDRAL ANGLE Conformers calculated total number: 60 / Conformers submitted total number: 20 |