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Yorodumi- EMDB-50107: Artificial membrane protein TMHC4_R (ROCKET) mutant R9A/K10A/R13A -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-50107 | |||||||||
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Title | Artificial membrane protein TMHC4_R (ROCKET) mutant R9A/K10A/R13A | |||||||||
Map data | ||||||||||
Sample |
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Keywords | syntectic construct / membrane protein / artificial protein | |||||||||
Biological species | synthetic construct (others) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.9 Å | |||||||||
Authors | Abramsson M / Anden O / Howard RJ / Lindahl E / Landreh M | |||||||||
Funding support | Sweden, 2 items
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Citation | Journal: To Be Published Title: Engineering cardiolipin binding to an artificial membrane protein Authors: Abramsson M / Corey RA / Skerle J / Persson L / Anden O / Oluwole AO / Howard RJ / Lindahl E / Robinson CV / Strisovsky K / Marklund EG / Drew D / Stansfeld PJ / Landreh M | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_50107.map.gz | 32.1 MB | EMDB map data format | |
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Header (meta data) | emd-50107-v30.xml emd-50107.xml | 14.5 KB 14.5 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_50107_fsc.xml | 18.3 KB | Display | FSC data file |
Images | emd_50107.png | 94.5 KB | ||
Masks | emd_50107_msk_1.map | 512 MB | Mask map | |
Filedesc metadata | emd-50107.cif.gz | 4.9 KB | ||
Others | emd_50107_half_map_1.map.gz emd_50107_half_map_2.map.gz | 404.2 MB 404.2 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-50107 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-50107 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_50107.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.5076 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_50107_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_50107_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_50107_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Artificial membrane protein TMHC4_R (ROCKET) mutant R9A/K10A/R13A
Entire | Name: Artificial membrane protein TMHC4_R (ROCKET) mutant R9A/K10A/R13A |
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Components |
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-Supramolecule #1: Artificial membrane protein TMHC4_R (ROCKET) mutant R9A/K10A/R13A
Supramolecule | Name: Artificial membrane protein TMHC4_R (ROCKET) mutant R9A/K10A/R13A type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: synthetic construct (others) |
Molecular weight | Theoretical: 99.56 kDa/nm |
-Macromolecule #1: TMHC4_R (ROCKET) mutant R9A/K10A/R13A
Macromolecule | Name: TMHC4_R (ROCKET) mutant R9A/K10A/R13A / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: synthetic construct (others) |
Recombinant expression | Organism: Escherichia coli BL21(DE3) (bacteria) |
Sequence | String: MSKDTEDSAA IWATIMLLLV FAILLSAIIW YQITTNPDTS QIATLLSMQL LLIALMLVVI ALLLSRQTEQ VAESIRRDVS ALAYVMLGLL LSLLNRLSLA AEAYKKAIEL DPNDALAWLL LGSVLEKLKR LDEAAEAYKK AIELKPNDAS AWKELGKVLE KLGRLDEAAE ...String: MSKDTEDSAA IWATIMLLLV FAILLSAIIW YQITTNPDTS QIATLLSMQL LLIALMLVVI ALLLSRQTEQ VAESIRRDVS ALAYVMLGLL LSLLNRLSLA AEAYKKAIEL DPNDALAWLL LGSVLEKLKR LDEAAEAYKK AIELKPNDAS AWKELGKVLE KLGRLDEAAE AYKKAIELDP EDAEAWKELG KVLEKLGRLD EAAEAYKKAI ELDPNDLEHH HHHH |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | 3D array |
-Sample preparation
Buffer | pH: 8 |
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Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.6 µm |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 60.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |