+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-44416 | ||||||||||||
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Title | Binned tomogram of stationary phase Burkholderia thailandensis | ||||||||||||
Map data | Binned tomogram of stationary phase Burkholderia thailandensis | ||||||||||||
Sample |
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Keywords | stationary phase / CELL CYCLE | ||||||||||||
Biological species | Burkholderia thailandensis E264 (bacteria) | ||||||||||||
Method | electron tomography / cryo EM | ||||||||||||
Authors | Khanna K / Welch MD | ||||||||||||
Funding support | United States, 3 items
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Citation | Journal: To Be Published Title: Cryo-electron tomography of stationary phase Burkholderia thailandensis Authors: Khanna K / Welch MD | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_44416.map.gz | 179.9 MB | EMDB map data format | |
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Header (meta data) | emd-44416-v30.xml emd-44416.xml | 32.6 KB 32.6 KB | Display Display | EMDB header |
Images | emd_44416.png | 177.2 KB | ||
Filedesc metadata | emd-44416.cif.gz | 3.9 KB | ||
Others | emd_44416_additional_1.map.gz emd_44416_additional_10.map.gz emd_44416_additional_11.map.gz emd_44416_additional_12.map.gz emd_44416_additional_2.map.gz emd_44416_additional_3.map.gz emd_44416_additional_4.map.gz emd_44416_additional_5.map.gz emd_44416_additional_6.map.gz emd_44416_additional_7.map.gz emd_44416_additional_8.map.gz emd_44416_additional_9.map.gz | 170.1 MB 364.9 MB 335.4 MB 372.9 MB 321 MB 146.6 MB 156.3 MB 158.4 MB 129.6 MB 111.1 MB 269.3 MB 311.8 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-44416 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-44416 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_44416.map.gz / Format: CCP4 / Size: 227 MB / Type: IMAGE STORED AS SIGNED BYTE | ||||||||||||||||||||
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Annotation | Binned tomogram of stationary phase Burkholderia thailandensis | ||||||||||||||||||||
Voxel size | X=Y=Z: 14.9 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
+Additional map: Binned tomogram of stationary phase Burkholderia thailandensis
+Additional map: Binned tomogram of stationary phase Burkholderia thailandensis
+Additional map: Binned tomogram of stationary phase Burkholderia thailandensis
+Additional map: Binned tomogram of stationary phase Burkholderia thailandensis
+Additional map: Binned tomogram of stationary phase Burkholderia thailandensis
+Additional map: Binned tomogram of stationary phase Burkholderia thailandensis
+Additional map: Binned tomogram of stationary phase Burkholderia thailandensis
+Additional map: Binned tomogram of stationary phase Burkholderia thailandensis
+Additional map: Binned tomogram of stationary phase Burkholderia thailandensis
+Additional map: Binned tomogram of stationary phase Burkholderia thailandensis
+Additional map: Binned tomogram of stationary phase Burkholderia thailandensis
+Additional map: Binned tomogram of stationary phase Burkholderia thailandensis
-Sample components
-Entire : Cryo-electron tomography of stationary phase Burkholderia thailan...
Entire | Name: Cryo-electron tomography of stationary phase Burkholderia thailandensis |
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Components |
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-Supramolecule #1: Cryo-electron tomography of stationary phase Burkholderia thailan...
Supramolecule | Name: Cryo-electron tomography of stationary phase Burkholderia thailandensis type: cell / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Burkholderia thailandensis E264 (bacteria) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | electron tomography |
Aggregation state | cell |
-Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
Sectioning | Focused ion beam - Instrument: OTHER / Focused ion beam - Ion: OTHER / Focused ion beam - Voltage: 30 / Focused ion beam - Current: 0.1 / Focused ion beam - Duration: 1800 / Focused ion beam - Temperature: 93 K / Focused ion beam - Initial thickness: 2000 / Focused ion beam - Final thickness: 150 Focused ion beam - Details: The value given for _em_focused_ion_beam.instrument is Aquilos 2. This is not in a list of allowed values {'OTHER', 'DB235'} so OTHER is written into the XML file. |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 6.0 µm / Nominal defocus min: 4.0 µm |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 100.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Number images used: 40 |
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