+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-38754 | |||||||||
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Title | Cryo-EM structure of the human 43S ribosome with PDCD4 | |||||||||
Map data | local resolution filtered map using Relion | |||||||||
Sample |
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Keywords | Ribosome / PDCD4 / eIF4A | |||||||||
Function / homology | Function and homology information epithelial to mesenchymal transition involved in cardiac fibroblast development / negative regulation of myofibroblast differentiation / positive regulation of mRNA binding / negative regulation of vascular associated smooth muscle cell differentiation / viral translational termination-reinitiation / positive regulation of mRNA cis splicing, via spliceosome / eukaryotic translation initiation factor 3 complex, eIF3e / cap-dependent translational initiation / eukaryotic translation initiation factor 3 complex, eIF3m / translation reinitiation ...epithelial to mesenchymal transition involved in cardiac fibroblast development / negative regulation of myofibroblast differentiation / positive regulation of mRNA binding / negative regulation of vascular associated smooth muscle cell differentiation / viral translational termination-reinitiation / positive regulation of mRNA cis splicing, via spliceosome / eukaryotic translation initiation factor 3 complex, eIF3e / cap-dependent translational initiation / eukaryotic translation initiation factor 3 complex, eIF3m / translation reinitiation / Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / : / IRES-dependent viral translational initiation / multi-eIF complex / eukaryotic translation initiation factor 3 complex / RNA cap binding / eukaryotic translation initiation factor 4F complex / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / eukaryotic 43S preinitiation complex / cytoplasmic translational initiation / translation factor activity, RNA binding / mRNA cap binding / formation of cytoplasmic translation initiation complex / Deadenylation of mRNA / negative regulation of JUN kinase activity / positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis / negative regulation of endoplasmic reticulum unfolded protein response / oxidized pyrimidine DNA binding / response to TNF agonist / positive regulation of base-excision repair / protein tyrosine kinase inhibitor activity / eukaryotic 48S preinitiation complex / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of respiratory burst involved in inflammatory response / positive regulation of gastrulation / metal-dependent deubiquitinase activity / IRE1-RACK1-PP2A complex / nucleolus organization / : / positive regulation of endodeoxyribonuclease activity / positive regulation of Golgi to plasma membrane protein transport / TNFR1-mediated ceramide production / M-decay: degradation of maternal mRNAs by maternally stored factors / negative regulation of RNA splicing / negative regulation of DNA repair / laminin receptor activity / response to alkaloid / positive regulation of endothelial cell apoptotic process / oxidized purine DNA binding / negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide / supercoiled DNA binding / neural crest cell differentiation / negative regulation of phagocytosis / NF-kappaB complex / rRNA modification in the nucleus and cytosol / ubiquitin-like protein conjugating enzyme binding / regulation of establishment of cell polarity / positive regulation of ubiquitin-protein transferase activity / regulation of translational initiation / Formation of the ternary complex, and subsequently, the 43S complex / erythrocyte homeostasis / cytoplasmic side of rough endoplasmic reticulum membrane / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / pigmentation / protein kinase A binding / Ribosomal scanning and start codon recognition / negative regulation of ubiquitin protein ligase activity / ion channel inhibitor activity / Translation initiation complex formation / positive regulation of vascular associated smooth muscle cell apoptotic process / phagocytic cup / positive regulation of mitochondrial depolarization / negative regulation of Wnt signaling pathway / positive regulation of T cell receptor signaling pathway / fibroblast growth factor binding / positive regulation of activated T cell proliferation / regulation of cell division / protein deubiquitination / Protein hydroxylation / TOR signaling / iron-sulfur cluster binding / BH3 domain binding / mTORC1-mediated signalling / SARS-CoV-1 modulates host translation machinery / Peptide chain elongation / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Selenocysteine synthesis / negative regulation of vascular associated smooth muscle cell proliferation / monocyte chemotaxis / Formation of a pool of free 40S subunits / cysteine-type endopeptidase activator activity involved in apoptotic process / ribosomal small subunit binding / positive regulation of cyclic-nucleotide phosphodiesterase activity / Eukaryotic Translation Termination / ribosomal small subunit export from nucleus / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.6 Å | |||||||||
Authors | Ye X / Huang Z / Li Y / Wang M / Cheng J | |||||||||
Funding support | 1 items
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Citation | Journal: Cell Res / Year: 2024 Title: Human tumor suppressor PDCD4 directly interacts with ribosomes to repress translation. Authors: Xianwen Ye / Zixuan Huang / Yi Li / Mengjiao Wang / Wanyu Meng / Maojian Miao / Jingdong Cheng / | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_38754.map.gz | 158.6 MB | EMDB map data format | |
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Header (meta data) | emd-38754-v30.xml emd-38754.xml | 81.9 KB 81.9 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_38754_fsc.xml | 14.9 KB | Display | FSC data file |
Images | emd_38754.png | 159.6 KB | ||
Filedesc metadata | emd-38754.cif.gz | 17.2 KB | ||
Others | emd_38754_additional_1.map.gz emd_38754_additional_2.map.gz emd_38754_additional_3.map.gz emd_38754_additional_4.map.gz emd_38754_additional_5.map.gz emd_38754_half_map_1.map.gz emd_38754_half_map_2.map.gz | 160 MB 158.1 MB 225.5 MB 161.2 MB 160.6 MB 225.4 MB 225.4 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-38754 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-38754 | HTTPS FTP |
-Related structure data
Related structure data | 8xxnMC 8xxlC 8xxmC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_38754.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | local resolution filtered map using Relion | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.146 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: this map come from a focusing refinement around...
File | emd_38754_additional_1.map | ||||||||||||
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Annotation | this map come from a focusing refinement around the PDCD4-eIF4A region, and local resolution filtered using Relion | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: multibody refinement map focusing eIF3 and local resolution...
File | emd_38754_additional_2.map | ||||||||||||
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Annotation | multibody refinement map focusing eIF3 and local resolution filtered using Relion | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: consensus map without post process
File | emd_38754_additional_3.map | ||||||||||||
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Annotation | consensus map without post process | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: multibody refinement map focusing 40S body and local...
File | emd_38754_additional_4.map | ||||||||||||
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Annotation | multibody refinement map focusing 40S body and local resolution filtered using Relion | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: multibody refinement map focusing 40S head and local...
File | emd_38754_additional_5.map | ||||||||||||
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Annotation | multibody refinement map focusing 40S head and local resolution filtered using Relion | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_38754_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_38754_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
+Entire : 43S ribosome with PDCD4
+Supramolecule #1: 43S ribosome with PDCD4
+Macromolecule #1: 60S ribosomal protein L41
+Macromolecule #3: 40S ribosomal protein SA
+Macromolecule #4: 40S ribosomal protein S3a
+Macromolecule #5: 40S ribosomal protein S3
+Macromolecule #6: 40S ribosomal protein S4, X isoform
+Macromolecule #7: 40S ribosomal protein S5
+Macromolecule #8: 40S ribosomal protein S7
+Macromolecule #9: 40S ribosomal protein S8
+Macromolecule #10: 40S ribosomal protein S10
+Macromolecule #11: 40S ribosomal protein S11
+Macromolecule #12: 40S ribosomal protein S15
+Macromolecule #13: 40S ribosomal protein S16
+Macromolecule #14: 40S ribosomal protein S17
+Macromolecule #15: 40S ribosomal protein S18
+Macromolecule #16: 40S ribosomal protein S19
+Macromolecule #17: 40S ribosomal protein S20
+Macromolecule #18: 40S ribosomal protein S21
+Macromolecule #19: 40S ribosomal protein S23
+Macromolecule #20: 40S ribosomal protein S26
+Macromolecule #21: 40S ribosomal protein S28
+Macromolecule #22: 40S ribosomal protein S29
+Macromolecule #23: Receptor of activated protein C kinase 1
+Macromolecule #24: 40S ribosomal protein S2
+Macromolecule #25: 40S ribosomal protein S6
+Macromolecule #26: 40S ribosomal protein S9
+Macromolecule #27: 40S ribosomal protein S12
+Macromolecule #28: 40S ribosomal protein S13
+Macromolecule #29: 40S ribosomal protein S14
+Macromolecule #30: 40S ribosomal protein S15a
+Macromolecule #31: 40S ribosomal protein S24
+Macromolecule #32: 40S ribosomal protein S25
+Macromolecule #33: 40S ribosomal protein S27
+Macromolecule #34: 40S ribosomal protein S30
+Macromolecule #35: Ubiquitin-40S ribosomal protein S27a
+Macromolecule #36: Eukaryotic translation initiation factor 1
+Macromolecule #37: Eukaryotic initiation factor 4A-I
+Macromolecule #38: Programmed cell death protein 4
+Macromolecule #39: Eukaryotic translation initiation factor 3 subunit A
+Macromolecule #40: Eukaryotic translation initiation factor 3 subunit B
+Macromolecule #41: Eukaryotic translation initiation factor 3 subunit C
+Macromolecule #42: Eukaryotic translation initiation factor 3 subunit E
+Macromolecule #43: Eukaryotic translation initiation factor 3 subunit F
+Macromolecule #44: Eukaryotic translation initiation factor 3 subunit G
+Macromolecule #45: Eukaryotic translation initiation factor 3 subunit H
+Macromolecule #46: Eukaryotic translation initiation factor 3 subunit I
+Macromolecule #47: Eukaryotic translation initiation factor 3 subunit K
+Macromolecule #48: Eukaryotic translation initiation factor 3 subunit L
+Macromolecule #49: Eukaryotic translation initiation factor 3 subunit M
+Macromolecule #50: Eukaryotic translation initiation factor 3 subunit D
+Macromolecule #2: 18S rRNA
+Macromolecule #51: MAGNESIUM ION
+Macromolecule #52: ZINC ION
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm |
Sample stage | Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |