National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01 GM120322
United States
American Heart Association
19POST34380439/Jingxian Li/2019-2020
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01 GM134035-01
United States
Citation
Journal: Nat Commun / Year: 2020 Title: Alternative splicing controls teneurin-latrophilin interaction and synapse specificity by a shape-shifting mechanism. Authors: Jingxian Li / Yuan Xie / Shaleeka Cornelius / Xian Jiang / Richard Sando / Szymon P Kordon / Man Pan / Katherine Leon / Thomas C Südhof / Minglei Zhao / Demet Araç / Abstract: The trans-synaptic interaction of the cell-adhesion molecules teneurins (TENs) with latrophilins (LPHNs/ADGRLs) promotes excitatory synapse formation when LPHNs simultaneously interact with FLRTs. ...The trans-synaptic interaction of the cell-adhesion molecules teneurins (TENs) with latrophilins (LPHNs/ADGRLs) promotes excitatory synapse formation when LPHNs simultaneously interact with FLRTs. Insertion of a short alternatively-spliced region within TENs abolishes the TEN-LPHN interaction and switches TEN function to specify inhibitory synapses. How alternative-splicing regulates TEN-LPHN interaction remains unclear. Here, we report the 2.9 Å resolution cryo-EM structure of the TEN2-LPHN3 complex, and describe the trimeric TEN2-LPHN3-FLRT3 complex. The structure reveals that the N-terminal lectin domain of LPHN3 binds to the TEN2 barrel at a site far away from the alternatively spliced region. Alternative-splicing regulates the TEN2-LPHN3 interaction by hindering access to the LPHN-binding surface rather than altering it. Strikingly, mutagenesis of the LPHN-binding surface of TEN2 abolishes the LPHN3 interaction and impairs excitatory but not inhibitory synapse formation. These results suggest that a multi-level coincident binding mechanism mediated by a cryptic adhesion complex between TENs and LPHNs regulates synapse specificity.
History
Deposition
Jan 9, 2020
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Header (metadata) release
Jun 3, 2020
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Map release
Jun 3, 2020
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Update
Dec 2, 2020
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Current status
Dec 2, 2020
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Entire : Binary complex of human Teneurin-2 with human Latrophilin-3 extra...
Entire
Name: Binary complex of human Teneurin-2 with human Latrophilin-3 extracellular domain
Components
Complex: Binary complex of human Teneurin-2 with human Latrophilin-3 extracellular domain
Protein or peptide: Teneurin-2Teneurin
Protein or peptide: Adhesion G protein-coupled receptor L3
Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
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Supramolecule #1: Binary complex of human Teneurin-2 with human Latrophilin-3 extra...
Supramolecule
Name: Binary complex of human Teneurin-2 with human Latrophilin-3 extracellular domain type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 Details: The binary complex includes human Teneurin2 (TENM2) (Teneurin without alternative splice sequence NKEFKHS) and human Latrophilin3 (LPHN3/ADGRL3) with an alternative splice sequence KVEQK ...Details: The binary complex includes human Teneurin2 (TENM2) (Teneurin without alternative splice sequence NKEFKHS) and human Latrophilin3 (LPHN3/ADGRL3) with an alternative splice sequence KVEQK between lectin domain and olfactomedin domain.
Source (natural)
Organism: Homo sapiens (human)
Recombinant expression
Organism: Trichoplusia ni (cabbage looper) / Recombinant cell: Hi5
Molecular weight
Experimental: 314 KDa
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Macromolecule #1: Teneurin-2
Macromolecule
Name: Teneurin-2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
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