+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDGZ9 |
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Sample | Protein Disulfide Isomerase (PDI) purified from bovine liver
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Function / homology | Function and homology information procollagen-proline 4-dioxygenase activity / protein disulfide-isomerase / peptidyl-proline hydroxylation to 4-hydroxy-L-proline / protein disulfide isomerase activity / response to endoplasmic reticulum stress / melanosome / protein folding / protein heterodimerization activity / external side of plasma membrane / endoplasmic reticulum lumen / endoplasmic reticulum Similarity search - Function |
Biological species | Bos taurus (cattle) |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Data source
SASBDB page | SASDGZ9 |
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-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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-External links
Related items in Molecule of the Month |
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-Models
Model #3863 | Type: dummy / Software: (dammif (r7883)) / Radius of dummy atoms: 3.50 A / Symmetry: P1 / Chi-square value: 1.296 / P-value: 0.050249 |
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Model #3874 | Type: dummy / Radius of dummy atoms: 3.50 A / Symmetry: P1 / Chi-square value: 1.296 / P-value: 0.050249 |
Model #3872 | Type: dummy / Software: (dammif (r7883)) / Radius of dummy atoms: 3.50 A / Symmetry: P1 / Chi-square value: 1.304 / P-value: 0.050249 |
Model #3875 | Type: dummy / Radius of dummy atoms: 3.50 A / Symmetry: P1 / Chi-square value: 1.304 / P-value: 0.050249 |
Model #3876 | Type: dummy / Software: (DAMFILT 5.0 (r7883)) / Radius of dummy atoms: 2.50 A / Symmetry: P1 / Chi-square value: 1.307 / P-value: 0.050249 |
-Sample
Sample | Name: Protein Disulfide Isomerase (PDI) purified from bovine liver Specimen concentration: 2.50-10.00 |
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Buffer | Name: 50 mM Tris-HCL, 150 mM NaCl / pH: 7.5 Comment: PDI was incubated with 0.5mM TCEP to reduce the Active sites one hour before SAXS data collection |
Entity #1937 | Name: PDI / Type: protein / Description: Protein disulfide-isomerase / Formula weight: 57.265 / Num. of mol.: 1 / Source: Bos taurus / References: UniProt: P05307 Sequence: MLRRALLCLA LTALFRAGAG APDEEDHVLV LHKGNFDEAL AAHKYLLVEF YAPWCGHCKA LAPEYAKAAG KLKAEGSEIR LAKVDATEES DLAQQYGVRG YPTIKFFKNG DTASPKEYTA GREADDIVNW LKKRTGPAAS TLSDGAAAEA LVESSEVAVI GFFKDMESDS ...Sequence: MLRRALLCLA LTALFRAGAG APDEEDHVLV LHKGNFDEAL AAHKYLLVEF YAPWCGHCKA LAPEYAKAAG KLKAEGSEIR LAKVDATEES DLAQQYGVRG YPTIKFFKNG DTASPKEYTA GREADDIVNW LKKRTGPAAS TLSDGAAAEA LVESSEVAVI GFFKDMESDS AKQFFLAAEV IDDIPFGITS NSDVFSKYQL DKDGVVLFKK FDEGRNNFEG EVTKEKLLDF IKHNQLPLVI EFTEQTAPKI FGGEIKTHIL LFLPKSVSDY EGKLSNFKKA AESFKGKILF IFIDSDHTDN QRILEFFGLK KEECPAVRLI TLEEEMTKYK PESDELTAEK ITEFCHRFLE GKIKPHLMSQ ELPDDWDKQP VKVLVGKNFE EVAFDEKKNV FVEFYAPWCG HCKQLAPIWD KLGETYKDHE NIVIAKMDST ANEVEAVKVH SFPTLKFFPA SADRTVIDYN GERTLDGFKK FLESGGQDGA GDDDDLEDLE EAEEPDLEED DDQKAVKDEL |
-Experimental information
Beam | Instrument name: Advanced Photon Source (APS), Argonne National Laboratory BioCAT 18ID City: Lemont, IL / 国: USA / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1033 Å / Dist. spec. to detc.: 3.485 mm | |||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus3 X 1M / Pixsize x: 0.172 mm | |||||||||||||||||||||||||||||||||
Scan | Title: Protein Disulfide Isomerase (PDI) purified from bovine liver Measurement date: Nov 4, 2015 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 8 / Unit: 1/A /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 856 /
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Result | Type of curve: merged Comments: PDI was purified from bovine liver and was incubated with 0.5mM TCEP to reduce the active sites one hour before SAXS data collection.
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