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- SASDFH6: Probable exodeoxyribonuclease III protein XthA, sliding beta clam... -

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Entry
Database: SASBDB / ID: SASDFH6
SampleProbable exodeoxyribonuclease III protein XthA, sliding beta clamp, DNA ligase-A tricomplex in the presence of DNA substrate
  • Probable exodeoxyribonuclease III protein XthA (protein), MtbXthA, Mycobacterium tuberculosis
  • DNA ligase A (protein), Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
  • Beta sliding clamp (protein), Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
  • DNA ligase A nicked DNA substrate (DNA)
Biological speciesMycobacterium tuberculosis (bacteria)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)

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Models

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Sample

SampleName: Probable exodeoxyribonuclease III protein XthA, sliding beta clamp, DNA ligase-A tricomplex in the presence of DNA substrate
Specimen concentration: 2.5 mg/ml / Entity id: 1670 / 1671 / 1672 / 1673
BufferName: 50 mM Tris pH 8.0, 200 mM NaCl , 2 mM β-mercaptoethanol
pH: 8
Entity #1670Name: MtbXthA / Type: protein / Description: Probable exodeoxyribonuclease III protein XthA / Formula weight: 30.699 / Num. of mol.: 1 / Source: Mycobacterium tuberculosis
Sequence: MRLATWNVNS VKARLPRLLA WLDETKPDVV CLQETKSAAD GFPSGDVAEL GYTTAAYGEG RWNGVAILSR VGIEDVSKSF PGEPVYQGEA PAGPGVQTQL TEPVLLAPQA RAIGATCGGI RVWSLYAPNG RTPESAHYLY KLEWFAALRD ALAAELSAGT PLVATGDYNV ...Sequence:
MRLATWNVNS VKARLPRLLA WLDETKPDVV CLQETKSAAD GFPSGDVAEL GYTTAAYGEG RWNGVAILSR VGIEDVSKSF PGEPVYQGEA PAGPGVQTQL TEPVLLAPQA RAIGATCGGI RVWSLYAPNG RTPESAHYLY KLEWFAALRD ALAAELSAGT PLVATGDYNV APTDDDVWDP SVFVGSTHVT PAERQALAEL RDLGLHDIVP TPMKGPHPYT YWDYRAGSFH KDLGMRIDLV YGNGAFAERV RSAYVDREAR KGRGPSDHAP VVVDLDHHHH HH
Entity #1671Type: protein / Description: DNA ligase A / Formula weight: 76.111 / Num. of mol.: 1
Source: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Sequence: MSSPDADQTA PEVLRQWQAL AEEVREHQFR YYVRDAPIIS DAEFDELLRR LEALEEQHPE LRTPDSPTQL VGGAGFATDF EPVDHLERML SLDNAFTADE LAAWAGRIHA EVGDAAHYLC ELKIDGVALS LVYREGRLTR ASTRGDGRTG EDVTLNARTI ADVPERLTPG ...Sequence:
MSSPDADQTA PEVLRQWQAL AEEVREHQFR YYVRDAPIIS DAEFDELLRR LEALEEQHPE LRTPDSPTQL VGGAGFATDF EPVDHLERML SLDNAFTADE LAAWAGRIHA EVGDAAHYLC ELKIDGVALS LVYREGRLTR ASTRGDGRTG EDVTLNARTI ADVPERLTPG DDYPVPEVLE VRGEVFFRLD DFQALNASLV EEGKAPFANP RNSAAGSLRQ KDPAVTARRR LRMICHGLGH VEGFRPATLH QAYLALRAWG LPVSEHTTLA TDLAGVRERI DYWGEHRHEV DHEIDGVVVK VDEVALQRRL GSTSRAPRWA IAYKYPPEEA QTKLLDIRVN VGRTGRITPF AFMTPVKVAG STVGQATLHN ASEIKRKGVL IGDTVVIRKA GDVIPEVLGP VVELRDGSER EFIMPTTCPE CGSPLAPEKE GDADIRCPNA RGCPGQLRER VFHVASRNGL DIEVLGYEAG VALLQAKVIA DEGELFALTE RDLLRTDLFR TKAGELSANG KRLLVNLDKA KAAPLWRVLV ALSIRHVGPT AARALATEFG SLDAIAAAST DQLAAVEGVG PTIAAAVTEW FAVDWHREIV DKWRAAGVRM VDERDESVPR TLAGLTIVVT GSLTGFSRDD AKEAIVARGG KAAGSVSKKT NYVVAGDSPG SKYDKAVELG VPILDEDGFR RLLADGPASR THHHHHH
Entity #1672Type: protein / Description: Beta sliding clamp / Formula weight: 42.935 / Num. of mol.: 2
Source: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Sequence: MDAATTRVGL TDLTFRLLRE SFADAVSWVA KNLPARPAVP VLSGVLLTGS DNGLTISGFD YEVSAEAQVG AEIVSPGSVL VSGRLLSDIT RALPNKPVDV HVEGNRVALT CGNARFSLPT MPVEDYPTLP TLPEETGLLP AELFAEAISQ VAIAAGRDDT LPMLTGIRVE ...Sequence:
MDAATTRVGL TDLTFRLLRE SFADAVSWVA KNLPARPAVP VLSGVLLTGS DNGLTISGFD YEVSAEAQVG AEIVSPGSVL VSGRLLSDIT RALPNKPVDV HVEGNRVALT CGNARFSLPT MPVEDYPTLP TLPEETGLLP AELFAEAISQ VAIAAGRDDT LPMLTGIRVE ILGETVVLAA TDRFRLAVRE LKWSASSPDI EAAVLVPAKT LAEAAKAGIG GSDVRLSLGT GPGVGKDGLL GISGNGKRST TRLLDAEFPK FRQLLPTEHT AVATMDVAEL IEAIKLVALV ADRGAQVRME FADGSVRLSA GADDVGRAEE DLVVDYAGEP LTIAFNPTYL TDGLSSLRSE RVSFGFTTAG KPALLRPVSG DDRPVAGLNG NGPFPAVSTD YVYLLMPVRL PGHHHHHH
Entity #1673Type: DNA / Description: DNA ligase A nicked DNA substrate / Formula weight: 8.1 / Num. of mol.: 2
Sequence:
GGTAGATCAG TGTCTATGTA TGTCAG

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.0992 Å / Dist. spec. to detc.: 2.9 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
ScanTitle: Probable exodeoxyribonuclease III protein XthA, sliding beta clamp, DNA ligase-A tricomplex in the presence of DNA substrate
Measurement date: May 17, 2017 / Storage temperature: 4 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /
MinMax
Q0.0308 4.9444
ResultType of curve: single_conc
Comments: The presented model shows the spatial alignment of several individually calculated models and does not fit the SAXS data. A corresponding individual model fit (individual model not provided ...Comments: The presented model shows the spatial alignment of several individually calculated models and does not fit the SAXS data. A corresponding individual model fit (individual model not provided for this entry) is shown. The sample is severely aggregated.
ExperimentalPorod
MW214 kDa-
Volume-496 nm3

P(R)GuinierGuinier error
Forward scattering, I0133 136.9 8
Radius of gyration, Rg5.818 nm5.76 nm0.33

MinMax
D-19.13
Guinier point38 43

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