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- SASDF97: Complex of Binary toxin receptor (Cqm1) with deuterated BinB comp... -

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Basic information

Entry
Database: SASBDB / ID: SASDF97
SampleComplex of Binary toxin receptor (Cqm1) with deuterated BinB component protein in 100% D2O
  • Binary toxin receptor Cqm1 protein (protein), Cqm1, synthetic construct
  • Deuterated BinB component of mosquito-larvicidal Binary toxin (protein), dBinB, synthetic construct
Biological speciessynthetic construct (others)
Contact author
  • Vinay Kumar (Bhabha Atomic Research Centre, Mumbai 400085, INDIA)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #3082
Type: dummy / Radius of dummy atoms: 1.90 A / Symmetry: P1
Comment: dBinB component is fully 'contrast matched' with 100% D2O,
Chi-square value: 0.318 / P-value: 0.086659
Model #3084
Type: atomic / Chi-square value: 0.348 / P-value: 0.000320

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Sample

SampleName: Complex of Binary toxin receptor (Cqm1) with deuterated BinB component protein in 100% D2O
Specimen concentration: 1 mg/ml / Entity id: 1691 / 1692
BufferName: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O / pH: 7.5 / Comment: SANS experiment were carried out in 100% D2O
Entity #1691Name: Cqm1 / Type: protein / Description: Binary toxin receptor Cqm1 protein / Formula weight: 64.665 / Num. of mol.: 1 / Source: synthetic construct
Sequence: MHHHHHHSSG VDLGTENLYF QSMREPDSKD WYQHATFYQI YPRSFLDSNG DGIGDLAGIT SKMKYLADIG IDATWLSPPF KSPLKDFGYD VSDFYAIQPE YGNLTDFDKL VEESHKNGIK LMLDFIPNHS SDQHEWFVKS VVRDPEYSDF YVWRPPATGG GPPNNWISVF ...Sequence:
MHHHHHHSSG VDLGTENLYF QSMREPDSKD WYQHATFYQI YPRSFLDSNG DGIGDLAGIT SKMKYLADIG IDATWLSPPF KSPLKDFGYD VSDFYAIQPE YGNLTDFDKL VEESHKNGIK LMLDFIPNHS SDQHEWFVKS VVRDPEYSDF YVWRPPATGG GPPNNWISVF GGSAWTYNQA RGEYYLHQFT PQQPDLNYRN PKVLAEMTKM LFFWLDRGVD GFRLDAINHM FEDEQFRDEP LSGWGQPGEY DSLDHIYTKD IPDVYDVVYN WRDQMDKYSA EKGRTIILMT EAYSSIEGTM LYYESADRKR QGAHMPFNFQ LIYDFKKEQN AVGLKSSIDW WMNNMPARHT PSWVAGSHDH SRVASRVGLD RVDQVMTLLH TLPGTSITYY GEEVAMQDFK EAQQFDNRDP NRTPMQWDSS TSAGFSTNTN TWLRVHPDYA RYNVDVMQKN PQSTFHHFQH LTKLRRHRTM QSGEYVHKTV GTKVYALLRE LRGEDSFLTV LNMAGAEDTV DLGDFVNLPQ KMRVEVAQPN SKSKAGNEVD ISKLTLGPYD SVVLRATVSS A
Entity #1692Name: dBinB / Type: protein
Description: Deuterated BinB component of mosquito-larvicidal Binary toxin
Formula weight: 56.4 / Num. of mol.: 1 / Source: synthetic construct
Sequence: MGSSHHHHHH SSGLVPRGSM CDSKDNSGVS EKCGKKFTNY PLNTTPTSLN YNLPEISKKF YNLKNKYSRN GYGLSKTEFP SSIENCPSNE YSIMYDNKDP RFLIRFLLDD GRYIIADRDD GEVFDEAPTY LDNNNHPIIS RHYTGEERQK FEQVGSGDYI TGEQFFQFYT ...Sequence:
MGSSHHHHHH SSGLVPRGSM CDSKDNSGVS EKCGKKFTNY PLNTTPTSLN YNLPEISKKF YNLKNKYSRN GYGLSKTEFP SSIENCPSNE YSIMYDNKDP RFLIRFLLDD GRYIIADRDD GEVFDEAPTY LDNNNHPIIS RHYTGEERQK FEQVGSGDYI TGEQFFQFYT QNKTRVLSNC RALDSRTILL STAKIFPIYP PASETQLTAF VNSSFYAAAI PQLPQTSLLE NIPEPTSLDD SGVLPKDAVR AVKGSALLPC IIVHDPNLNN SDKMKFNTYY LLEYKEYWHQ LWSQIIPAHQ TVKIQERTGI SEVVQNSMIE DLNMYIGADF GMLFYFRSSG FKEQITRGLN RPLSQTTTQL GERVEEMEYY NSNDLDVRYV KYALAREFTL KRVNGEIVKN WVAVDYRLAG IQSYPNAPIT NPLTLTKHTI IRCENSYDGH IFKTPLIFKN GEVIVKTNEE LIPKINQ

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Experimental information

BeamInstrument name: Dhruva Reactor, Bhabha Atomic Research Centre SANS-I facility
City: Mumbai / 国: India / Type of source: neutron source / Wavelength: 0.52 Å / Dist. spec. to detc.: 2 mm
DetectorName: Ingenuously built / Type: 1 m long He3 position sensitive detector / Pixsize x: 12 mm
Scan
Title: Complex of Binary toxin receptor (Cqm1) with deuterated BinB component protein in 100% D2O
Measurement date: Apr 23, 2019 / Storage temperature: 25 °C / Cell temperature: 25 °C / Unit: 1/A /
MinMax
Q0.0162 0.1991
Distance distribution function P(R)
Sofotware P(R): GNOM 4.5a / Number of points: 100 /
MinMax
Q0.01619 0.1991
P(R) point1 100
R0 72.9
Result
Type of curve: single_conc
Comments: This data is for a stable complex of receptor Cqm1 protein and deuterated BinB component of mosquito-larvicidal Binary (BinAB) toxin. Deuterated BinB (dBinB) protein was synthesized using ...Comments: This data is for a stable complex of receptor Cqm1 protein and deuterated BinB component of mosquito-larvicidal Binary (BinAB) toxin. Deuterated BinB (dBinB) protein was synthesized using E. coli based protein expression system grown in M9+ medium in D2O after three step adaption. The extent of deuteration for dBinB was estimated from contrast (p − s)2 values to be ~77%. Theoretical molecular weight of the dBinB protein includes 77% of D atoms. SANS analysis (DAMMIN and CRYSON fit) suggest that deuterated BinB (dBinB), expectedly, is fully matched out against the used solvent (100% D2O) and only monomer of Cqm1 is modelled from SANS experiments
ExperimentalStandard
MW70.6 kDa66.5 kDa

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.04963 0.0022 0.048 0.0013
Radius of gyration, Rg2.18 nm0.121 1.87 nm0.43

MinMax
D-7.29
Guinier point1 29

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