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- SASDF87: Mosqutio-larvicidal Binary (BinAB) toxin Receptor Cqm1 protein in... -

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Basic information

Entry
Database: SASBDB / ID: SASDF87
SampleMosqutio-larvicidal Binary (BinAB) toxin Receptor Cqm1 protein in 100% D2O
  • Binary toxin receptor Cqm1 protein (protein), Cqm1, synthetic construct
Biological speciessynthetic construct (others)
Contact author
  • Vinay Kumar (Bhabha Atomic Research Centre, Mumbai 400085, INDIA)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #3074
Type: dummy / Radius of dummy atoms: 2.30 A / Symmetry: P2 / Chi-square value: 0.300 / P-value: 0.043668
Model #3076
Type: atomic
Comment: X-ray structure has recently been resolved by us. Given model is a dimer of observed tetramer.
Chi-square value: 0.326 / P-value: 0.043668

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Sample

SampleName: Mosqutio-larvicidal Binary (BinAB) toxin Receptor Cqm1 protein in 100% D2O
Specimen concentration: 3.3 mg/ml
BufferName: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O / pH: 7.5
Entity #1689Name: Cqm1 / Type: protein / Description: Binary toxin receptor Cqm1 protein / Formula weight: 64.67 / Num. of mol.: 2 / Source: synthetic construct
Sequence: MHHHHHHSSG VDLGTENLYF QSMREPDSKD WYQHATFYQI YPRSFLDSNG DGIGDLAGIT SKMKYLADIG IDATWLSPPF KSPLKDFGYD VSDFYAIQPE YGNLTDFDKL VEESHKNGIK LMLDFIPNHS SDQHEWFVKS VVRDPEYSDF YVWRPPATGG GPPNNWISVF ...Sequence:
MHHHHHHSSG VDLGTENLYF QSMREPDSKD WYQHATFYQI YPRSFLDSNG DGIGDLAGIT SKMKYLADIG IDATWLSPPF KSPLKDFGYD VSDFYAIQPE YGNLTDFDKL VEESHKNGIK LMLDFIPNHS SDQHEWFVKS VVRDPEYSDF YVWRPPATGG GPPNNWISVF GGSAWTYNQA RGEYYLHQFT PQQPDLNYRN PKVLAEMTKM LFFWLDRGVD GFRLDAINHM FEDEQFRDEP LSGWGQPGEY DSLDHIYTKD IPDVYDVVYN WRDQMDKYSA EKGRTIILMT EAYSSIEGTM LYYESADRKR QGAHMPFNFQ LIYDFKKEQN AVGLKSSIDW WMNNMPARHT PSWVAGSHDH SRVASRVGLD RVDQVMTLLH TLPGTSITYY GEEVAMQDFK EAQQFDNRDP NRTPMQWDSS TSAGFSTNTN TWLRVHPDYA RYNVDVMQKN PQSTFHHFQH LTKLRRHRTM QSGEYVHKTV GTKVYALLRE LRGEDSFLTV LNMAGAEDTV DLGDFVNLPQ KMRVEVAQPN SKSKAGNEVD ISKLTLGPYD SVVLRATVSS A

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Experimental information

BeamInstrument name: Dhruva Reactor, Bhabha Atomic Research Centre SANS-I facility
City: Mumbai / 国: India / Type of source: neutron source / Wavelength: 0.52 Å / Dist. spec. to detc.: 2 mm
DetectorName: Ingenuously built / Type: 1 m long He3 position sensitive detector / Pixsize x: 12 mm
Scan
Title: Mosqutio-larvicidal Binary (BinAB) toxin Receptor Cqm1 protein in 100% D2O
Measurement date: Apr 24, 2019 / Storage temperature: 25 °C / Cell temperature: 25 °C / Unit: 1/A /
MinMax
Q0.0162 0.1991
Distance distribution function P(R)
Sofotware P(R): GNOM 4.5a / Number of points: 100 /
MinMax
Q0.01619 0.1991
P(R) point1 100
R0 96.6
Result
Type of curve: single_conc
Comments: Molecular weight estimated from experimental data using I(0) value from GNOM analysis. Submitted SANS data has been normalized to protein concentration of 1 mg/ml. Protein (3.33 mg/ml) was ...Comments: Molecular weight estimated from experimental data using I(0) value from GNOM analysis. Submitted SANS data has been normalized to protein concentration of 1 mg/ml. Protein (3.33 mg/ml) was used during SANS experiments
ExperimentalStandard
MW120 kDa114 kDa

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.08495 0.001307 0.082 8.2E-5
Radius of gyration, Rg3.08 nm-2.82 nm0.103

MinMax
D-9.66
Guinier point1 17

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