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- SASDEU4: ACA8 complex with Calmodulin (hydrogenated) in stealth nanodisc (... -

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Basic information

Entry
Database: SASBDB / ID: SASDEU4
SampleACA8 complex with Calmodulin (hydrogenated) in stealth nanodisc (SANS, 100% D2O)
  • Membrane scaffold protein 1D1 (deuterated, 75%) (protein), d75-msp1d1
  • 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) (other), d(78,92)-POPC, Escherichia coli. (AL95 strain)
  • Calcium-transporting ATPase 8, plasma membrane-type (protein), ACA8, Arabidopsis thaliana
  • Calmodulin-7 (protein), Cam7, Arabidopsis thaliana
Biological speciesEscherichia coli. (AL95 strain)
Arabidopsis thaliana (thale cress)

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Structure visualization

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Models

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Sample

SampleName: ACA8 complex with Calmodulin (hydrogenated) in stealth nanodisc (SANS, 100% D2O)
Contrast: -3.32 / Specific vol: 0.744 / Specimen concentration: 3 mg/ml / Concentration method: A280 / Entity id: 1017 / 1018 / 1312 / 1314
BufferName: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2 / pH: 7.5
Entity #1017Name: d75-msp1d1 / Type: protein
Description: Membrane scaffold protein 1D1 (deuterated, 75%)
Formula weight: 24.661 / Num. of mol.: 2
Sequence: GHHHHHHHDY DIPTTENLYF QGSTFSKLRE QLGPVTQEFW DNLEKETEGL RQEMSKDLEE VKAKVQPYLD DFQKKWQEEM ELYRQKVEPL RAELQEGARQ KLHELQEKLS PLGEEMRDRA RAHVDALRTH LAPYSDELRQ RLAARLEALK ENGGARLAEY HAKATEHLST ...Sequence:
GHHHHHHHDY DIPTTENLYF QGSTFSKLRE QLGPVTQEFW DNLEKETEGL RQEMSKDLEE VKAKVQPYLD DFQKKWQEEM ELYRQKVEPL RAELQEGARQ KLHELQEKLS PLGEEMRDRA RAHVDALRTH LAPYSDELRQ RLAARLEALK ENGGARLAEY HAKATEHLST LSEKAKPALE DLRQGLLPVL ESFKVSFLSA LEEYTKKLNT Q
Entity #1018Name: d(78,92)-POPC / Type: other
Description: 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)
Formula weight: 0.76 / Source: Escherichia coli. (AL95 strain)
Sequence:
C42H82NO8P
Entity #1312Name: ACA8 / Type: protein
Description: Calcium-transporting ATPase 8, plasma membrane-type
Formula weight: 118.209 / Num. of mol.: 1 / Source: Arabidopsis thaliana
Sequence: HHHHHHHHEN LYFQGSMTSL LKSSPGRRRG GDVESGKSEH ADSDSDTFYI PSKNASIERL QQWRKAALVL NASRRFRYTL DLKKEQETRE MRQKIRSHAH ALLAANRFMD MGRESGVEKT TGPATPAGDF GITPEQLVIM SKDHNSGALE QYGGTQGLAN LLKTNPEKGI ...Sequence:
HHHHHHHHEN LYFQGSMTSL LKSSPGRRRG GDVESGKSEH ADSDSDTFYI PSKNASIERL QQWRKAALVL NASRRFRYTL DLKKEQETRE MRQKIRSHAH ALLAANRFMD MGRESGVEKT TGPATPAGDF GITPEQLVIM SKDHNSGALE QYGGTQGLAN LLKTNPEKGI SGDDDDLLKR KTIYGSNTYP RKKGKGFLRF LWDACHDLTL IILMVAAVAS LALGIKTEGI KEGWYDGGSI AFAVILVIVV TAVSDYKQSL QFQNLNDEKR NIHLEVLRGG RRVEISIYDI VVGDVIPLNI GNQVPADGVL ISGHSLALDE SSMTGESKIV NKDANKDPFL MSGCKVADGN GSMLVTGVGV NTEWGLLMAS ISEDNGEETP LQVRLNGVAT FIGSIGLAVA AAVLVILLTR YFTGHTKDNN GGPQFVKGKT KVGHVIDDVV KVLTVAVTIV VVAVPEGLPL AVTLTLAYSM RKMMADKALV RRLSACETMG SATTICSDKT GTLTLNQMTV VESYAGGKKT DTEQLPATIT SLVVEGISQN TTGSIFVPEG GGDLEYSGSP TEKAILGWGV KLGMNFETAR SQSSILHAFP FNSEKKRGGV AVKTADGEVH VHWKGASEIV LASCRSYIDE DGNVAPMTDD KASFFKNGIN DMAGRTLRCV ALAFRTYEAE KVPTGEELSK WVLPEDDLIL LAIVGIKDPC RPGVKDSVVL CQNAGVKVRM VTGDNVQTAR AIALECGILS SDADLSEPTL IEGKSFREMT DAERDKISDK ISVMGRSSPN DKLLLVQSLR RQGHVVAVTG DGTNDAPALH EADIGLAMGI AGTEVAKESS DIIILDDNFA SVVKVVRWGR SVYANIQKFI QFQLTVNVAA LVINVVAAIS SGDVPLTAVQ LLWVNLIMDT LGALALATEP PTDHLMGRPP VGRKEPLITN IMWRNLLIQA IYQVSVLLTL NFRGISILGL EHEVHEHATR VKNTIIFNAF VLCQAFNEFN ARKPDEKNIF KGVIKNRLFM GIIVITLVLQ VIIVEFLGKF ASTTKLNWKQ WLICVGIGVI SWPLALVGKF IPVPAAPISN KLKVLKFWGK KKNSSGEGSL
Entity #1314Name: Cam7 / Type: protein / Description: Calmodulin-7 / Num. of mol.: 1 / Source: Arabidopsis thaliana

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Experimental information

BeamInstrument name: Heinz Maier-Leibnitz Zentrum SANS-1 / City: Garching / : Germany / Type of source: neutron source / Wavelength: 5 Å / Dist. spec. to detc.: 5.5 mm
DetectorName: MLZ / Type: Array of 128 3-He position sensitive tubes / Pixsize x: 8 mm
ScanTitle: ACA8 + Cam7 in stealth nanodisc (SANS, 100% D2O) / Measurement date: Aug 28, 2017 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 7200 sec. / Number of frames: 1 / Unit: 1/A /
MinMax
Q0.0119 0.2336
ResultType of curve: single_conc
Comments: 'ACA8 protein complex with Calmodulin encapsulated in a partially deuterated
ExperimentalExperimental errorPorodPorod error
MW186 kDa20 186 kDa20
Volume--297 nm330

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.15 0.001 0.15 0.001
Radius of gyration, Rg5.29 nm0.01 4.78 nm0.01

MinMaxError
D-18 0.5
Guinier point4 9 -

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