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- SASDEB9: Flagellar brake protein YcgR from Escherichia coli -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDEB9
SampleFlagellar brake protein YcgR from Escherichia coli
  • Flagellar brake protein YcgR (protein), Escherichia coli (strain K12)
Function / homology
Function and homology information


regulation of bacterial-type flagellum-dependent cell motility by regulation of motor speed / negative regulation of bacterial-type flagellum-dependent cell motility / bacterial-type flagellum basal body / cyclic-di-GMP binding / bacterial-type flagellum-dependent cell motility
Similarity search - Function
Type III secretion system flagellar brake protein YcgR / Flagellar regulator YcgR, PilZN domain / Type III secretion system flagellar brake protein YcgR, PilZN domain / PilZ domain / PilZ domain / FMN-binding split barrel
Similarity search - Domain/homology
Flagellar brake protein YcgR
Similarity search - Component
Biological speciesEscherichia coli (strain K12) (bacteria)
Contact author
  • Yanjie Hou (Institute of Biophysics, Chinese Academy of Sciences)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2729
Type: dummy / Radius of dummy atoms: 3.30 A / Chi-square value: 1.032 / P-value: 0.072997
Search similar-shape structures of this assembly by Omokage search (details)
Model #2730
Type: atomic / Chi-square value: 2.56 / P-value: 0.000005
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Flagellar brake protein YcgR from Escherichia coli / Specimen concentration: 2 mg/ml
BufferName: 20 mM HEPES, 150mM NaCl, 10% glycerol, / pH: 7.5
Entity #1417Type: protein / Description: Flagellar brake protein YcgR / Formula weight: 29.376 / Num. of mol.: 1 / Source: Escherichia coli (strain K12) / References: UniProt: P76010
Sequence: MSHYHEQFLK QNPLAVLGVL RDLHKAAIPL RLSWNGGQLI SKLLAITPDK LVLDFGSQAE DNIAVLKAQH ITITAETQGA KVEFTVEQLQ QSEYLQLPAF ITVPPPTLWF VQRRRYFRIS APLHPPYFCQ TKLADNSTLR FRLYDLSLGG MGALLETAKP AELQEGMRFA ...Sequence:
MSHYHEQFLK QNPLAVLGVL RDLHKAAIPL RLSWNGGQLI SKLLAITPDK LVLDFGSQAE DNIAVLKAQH ITITAETQGA KVEFTVEQLQ QSEYLQLPAF ITVPPPTLWF VQRRRYFRIS APLHPPYFCQ TKLADNSTLR FRLYDLSLGG MGALLETAKP AELQEGMRFA QIEVNMGQWG VFHFDAQLIS ISERKVIDGK NETITTPRLS FRFLNVSPTV ERQLQRIIFS LEREAREKAD KVRDKLAAAL EHHHHHH

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Experimental information

BeamInstrument name: Shanghai Synchrotron Radiation Facility (SSRF) BL19U2
City: Shanghai / : China / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.103 Å
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Flagellar brake protein YcgR from Escherichia coli / Measurement date: Jan 4, 2016 / Storage temperature: 4 °C / Cell temperature: 4 °C / Exposure time: 1 sec. / Number of frames: 20 / Unit: 1/A /
MinMax
Q0.0232 0.3341
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 631 /
MinMax
Q0.0232228 0.334132
P(R) point1 631
R0 91
Result
Type of curve: single_conc /
ExperimentalPorod
MW29 kDa27.7 kDa
Volume-44.33 nm3

P(R)GuinierGuinier error
Forward scattering, I02190 2183.73 9.59
Radius of gyration, Rg2.67 nm2.61 nm0.3

MinMax
D-9.1
Guinier point1 54

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