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- SASDDB7: Synthetic FRET biosensor Twitch 2B N532F Ca-bound (Synthetic FRET... -

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Entry
Database: SASBDB / ID: SASDDB7
SampleSynthetic FRET biosensor Twitch 2B N532F Ca-bound
  • Synthetic FRET biosensor Twitch2B N532F (protein), TW6, synthetic construct
Biological speciessynthetic construct (others)
Contact author
  • Pablo Trigo Mourino (Max Planck Institute for Biophysical Chemistry)

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Sample

SampleName: Synthetic FRET biosensor Twitch 2B N532F Ca-bound / Specimen concentration: 5 mg/ml
BufferName: 20 mM MOPS 100 mM NaCl 5 mM CaCl2 / pH: 7 / Comment: Buffer A
Entity #1087Name: TW6 / Type: protein / Description: Synthetic FRET biosensor Twitch2B N532F / Formula weight: 62.432 / Num. of mol.: 1 / Source: synthetic construct
Sequence: MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLSWGVQ CFARYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNAIHGN VYITADKQKN GIKANFGLNC ...Sequence:
MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLSWGVQ CFARYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNAIHGN VYITADKQKN GIKANFGLNC NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSKLSK DPNEKRDHMV LLEFVTAARM QVADASEEEL SECFRIFDFD GNGFIDREEF GDIIRLTGEQ LTDEDVDEIF GDSDTDKNGR IDFDEFLKMV ENVQPIYPEL MGGVQLADHY QQNTPIGDGP VLLPDNHYLS YQSKLSKDPN EKRDHMVLLE FVTAAGITLG MDELYKGGTG GSMVSKGEEL FTGVVPILVE LDGDVNGHKF SVRGEGEGDA TNGKLTLKLI CTTGKLPVPW PTLVTTLGYG LMCFARYPDH MKQHDFFKSA MPEGYVQERT IFFKDDGNYK TRAEVKFEGD TLVNRIELKG IDFKEDGNIL GHKLEYNYNS HFVYITADKQ KNGIKANFKI RHNIED

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.0995 Å / Dist. spec. to detc.: 2.867 mm
DetectorName: Pilatus 1M
Scan
Title: Synthetic FRET biosensor Twitch 2B N532F Ca-bound / Measurement date: Jun 30, 2016 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 12 / Unit: 1/nm /
MinMax
Q0.0346 4.9518
ResultExperimental MW: 56.05 kDa / I0 from Guinier: 37.44 / Rg from Guinier: 2.76 nm / Type of curve: single_conc /
MinMax
Guinier point13 62

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