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- SASDCY6: T2 in b-DDM micelle (SEC-SAXS) (Mechanosensitive channel T2, T2 +... -

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Basic information

Entry
Database: SASBDB / ID: SASDCY6
SampleT2 in b-DDM micelle (SEC-SAXS)
  • Mechanosensitive channel T2 (protein), T2, Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
  • n-Dodecyl-β-D-Maltopyranoside (other), b-DDM, Synthetic compound
Function / homologyMechanosensitive ion channel MscS, transmembrane-2 / Mechanosensitive ion channel MscS / Mechanosensitive ion channel, beta-domain / LSM domain superfamily / transmembrane transport / membrane => GO:0016020 / Uncharacterized protein
Function and homology information
Biological speciesThermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) (archaea)
Synthetic compound (others)
CitationDate: 2018 Feb
Title: Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research
Authors: Flayhan A / Mertens H / Ural-Blimke Y / Martinez Molledo M / Svergun D
Contact author
  • Haydyn Mertens (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #1498
Type: dummy / Software: (2.9.0) / Radius of dummy atoms: 3.50 A / Symmetry: P7 / Chi-square value: 3.360
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: T2 in b-DDM micelle (SEC-SAXS) / Entity id: 786 / 789
BufferName: 20 mM Tris 150 mM NaCl 5 % glycerol 0.03 % DDM / pH: 7.5
Entity #786Name: T2 / Type: protein / Description: Mechanosensitive channel T2 / Formula weight: 32.887 / Num. of mol.: 7
Source: Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
References: UniProt: Q97AH5
Sequence: MDRAKGKSIA VYVGSMIVLI IVLLALLYVL SLFHIVPIKF SRILYAVVIG LVIYAIVRIV TKYVERFISL HSEFKHLKYI VFIISLLGYF IISLAVLAAL GIDVSSVILG SAFLSAIIGL AAQSVLANVF GGLFLSIVRP FKLGDHIVVN TWQFPVSFPS YPPKYFSRDF ...Sequence:
MDRAKGKSIA VYVGSMIVLI IVLLALLYVL SLFHIVPIKF SRILYAVVIG LVIYAIVRIV TKYVERFISL HSEFKHLKYI VFIISLLGYF IISLAVLAAL GIDVSSVILG SAFLSAIIGL AAQSVLANVF GGLFLSIVRP FKLGDHIVVN TWQFPVSFPS YPPKYFSRDF IEGSIYRGIV SDISINYTSI ALESGDVVKI PNNIMIQAAI TLRHGSVTVQ ARYEVPKYIN FNDIYNKIVD EVGTLPGARN IGVMIDETTV NTYIILVRGD FQGEDADLVR SQILQKVIGI VEPLKKQ
Entity #789Name: b-DDM / Type: other / Description: n-Dodecyl-β-D-Maltopyranoside / Formula weight: 0.511 / Source: Synthetic compound

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Experimental information

BeamInstrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 2M
Scan
Title: T2_DDM / Measurement date: May 29, 2016 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 3600 / Unit: 1/nm /
MinMax
Q0.0778 4.607
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1000 /
MinMax
Q0.0778114 2.80185
P(R) point1 1000
R0 16.5
Result
Type of curve: single_conc
ExperimentalExperimental errorPorodPorod error
MW355 kDa40 560 kDa60
Volume--896 nm390

P(R)P(R) errorGuinierGuinier error
Forward scattering, I0250 5 251 5
Radius of gyration, Rg5.6 nm0.2 5.6 nm0.1

MinMaxError
D-16.5 0.5
Guinier point1 30 -

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