SASDAU4: uPAR H47C/N259C (Urokinase plasminogen activator surface receptor...

Basic information

• Entry: Database: SASBDB / ID: SASDAU4

Sample

uPAR H47C/N259C

• Urokinase plasminogen activator surface receptorUrokinase receptor (protein), uPARUrokinase receptor, Homo sapiens

Function / homology

Function:

urokinase plasminogen activator receptor activity / Attachment of GPI anchor to uPAR / positive regulation of homotypic cell-cell adhesion / negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway / urokinase plasminogen activator signaling pathway / regulation of plasminogen activation / regulation of fibrinolysis / protein complex involved in cell-matrix adhesion / regulation of proteolysis / serine-type endopeptidase complex / Dissolution of Fibrin Clot / positive regulation of epidermal growth factor receptor signaling pathway / extrinsic component of membrane / positive regulation of release of cytochrome c from mitochondria / positive regulation of DNA binding / regulation of cell adhesion / specific granule membrane / negative regulation of intrinsic apoptotic signaling pathway / cell projection / chemotaxis / blood coagulation / signaling receptor activity / positive regulation of protein phosphorylation / protein domain specific binding / external side of plasma membrane / endoplasmic reticulum lumen / signaling receptor binding / focal adhesion / Neutrophil degranulation / endoplasmic reticulum membrane / negative regulation of apoptotic process / enzyme binding / cell surface / signal transduction / extracellular region / membrane / plasma membrane

Domain/homology:

CD59 antigen, conserved site / Ly-6 / u-PAR domain signature. / Ly-6 antigen / uPA receptor -like domain / u-PAR/Ly-6 domain / Ly-6 antigen/uPA receptor-like / Snake toxin-like superfamily

Component:

Urokinase plasminogen activator surface receptor

• Biological species: Homo sapiens (human)
• Citation: Journal: J Biol Chem / Year: 2012; Title: A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR).; Authors: Haydyn D T Mertens / Magnus Kjaergaard / Simon Mysling / Henrik Gårdsvoll / Thomas J D Jørgensen / Dmitri I Svergun / Michael Ploug / ; Abstract: The urokinase-type plasminogen activator receptor (uPAR) provides a rendezvous between proteolytic degradation of the extracellular matrix and integrin-mediated adhesion to vitronectin. These processes are, however, tightly linked because the high affinity binding of urokinase regulates the binding of uPAR to matrix-embedded vitronectin. Although crystal structures exist to define the corresponding static bi- and trimolecular receptor complexes, it is evident that the dynamic property of uPAR plays a decisive role in its function. In the present study, we combine small angle x-ray scattering, hydrogen-deuterium exchange, and surface plasmon resonance to develop a structural model describing the allosteric regulation of uPAR. We show that the flexibility of its N-terminal domain provides the key for understanding this allosteric mechanism. Importantly, our model has direct implications for understanding uPAR-assisted cell adhesion and migration as well as for translational research, including targeted intervention therapy and non-invasive tumor imaging in vivo.

Contact author

• Haydyn Mertens (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

Models

• Model #78: ; Type: dummy / Software: dammif / Radius of dummy atoms: 1.90 A / Symmetry: P1 / Chi-square value: 2.217121; Search similar-shape structures of this assembly by Omokage search (details)
• Model #80: ; Type: mix / Software: coral / Radius of dummy atoms: 1.90 A / Symmetry: P1 / Chi-square value: 1.787569; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: uPAR H47C/N259C / Sample MW: 36.98 kDa / Specimen concentration: 1.50-4.40 / Concentration method: A280
• Buffer: Name: Sodium Phosphate / Concentration: 25.00 mM / pH: 7.2 / Composition: Glycerol 5.000 %, NaSO4 50.000 mM

Entity #66

Name: uPARUrokinase receptor / Type: protein
Description: Urokinase plasminogen activator surface receptorUrokinase receptor
Formula weight: 36.98 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q03405
Sequence:

MGHPPLLPLL LLLHTCVPAS WGLRCMQCKT NGDCRVEECA LGQDLCRTTI VRLWEEGEEL ELVEKSCTHS EKTNRTLSYR TGLKITSLTE VVCGLDLCNQ GNSGRAVTYS RSRYLECISC GSSDMSCERG RHQSLQCRSP EEQCLDVVTH WIQEGEEGRP KDDRHLRGCG YLPGCPGSNG FHNNDTFHFL KCCNTTKCNE GPILELENLP QNGRQCYSCK GNSTHGCSSE ETFLIDCRGP MNQCLVATGT HEPKNQSYMV RGCATASMCQ HAHLGDAFSM NHIDVSCCTK SGCNHPDLDV QYRSGAAPQP GPAHLSLTIT LLMTARLWGG TLLWT

Experimental information

• Beam: Instrument name: DORIS III X33 / City: Hamburg / 国: Germany / Shape: 0.6 / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.15 Å / Dist. spec. to detc.: 2.7 mm
• Detector: Name: Pilatus 1M-W / Pixsize x: 0.172 mm

Scan

Title: uPAR H47C/N259C / Measurement date: Jun 10, 2010 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 30 sec. / Number of frames: 4 / Unit: 1/nm /

	Min	Max
Q	0.0675	6.3452

Distance distribution function P(R)

Sofotware P(R): GNOM 4.5a / Number of points: 507 /

	Min	Max
Q	0.1949	2.914
P(R) point	48	554
R	0	7

Result

Type of curve: merged / Standard: BSA /

	Experimental	Standard	Porod
MW	34 kDa	34 kDa	-
Volume	-	-	57 nm3

	Guinier	P(R)
Forward scattering, I0	36.7	-
Radius of gyration, Rg	2.2 nm	2.2 nm

	Min	Max
D	-	7
Guinier point	48	192