+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 8jty | ||||||
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タイトル | hOCT1 in complex with nb5660 in inward facing partially open 2 conformation | ||||||
要素 |
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キーワード | MEMBRANE PROTEIN (膜タンパク質) / SLC / transporter (運搬体タンパク質) | ||||||
機能・相同性 | 機能・相同性情報 acetylcholine transmembrane transporter activity / secondary active organic cation transmembrane transporter activity / putrescine transmembrane transporter activity / (R)-carnitine transmembrane transporter activity / acyl carnitine transmembrane transport / pyrimidine nucleoside transmembrane transporter activity / acetylcholine transport / serotonin transport / spermidine transmembrane transporter activity / quaternary ammonium group transmembrane transporter activity ...acetylcholine transmembrane transporter activity / secondary active organic cation transmembrane transporter activity / putrescine transmembrane transporter activity / (R)-carnitine transmembrane transporter activity / acyl carnitine transmembrane transport / pyrimidine nucleoside transmembrane transporter activity / acetylcholine transport / serotonin transport / spermidine transmembrane transporter activity / quaternary ammonium group transmembrane transporter activity / quaternary ammonium group transport / epinephrine transport / thiamine transmembrane transport / purine-containing compound transmembrane transport / spermidine transport / thiamine transport / thiamine transmembrane transporter activity / norepinephrine:sodium symporter activity / organic cation transport / Organic cation transport / organic cation transmembrane transporter activity / putrescine transport / dopamine uptake / metanephric proximal tubule development / dopamine:sodium symporter activity / norepinephrine transport / prostaglandin transport / : / prostaglandin transmembrane transporter activity / neurotransmitter transmembrane transporter activity / Norepinephrine Neurotransmitter Release Cycle / toxin transmembrane transporter activity / serotonin uptake / Abacavir transmembrane transport / Na+/Cl- dependent neurotransmitter transporters / monoamine transmembrane transporter activity / dopamine transport / monoamine transport / organic anion transmembrane transporter activity / establishment or maintenance of transmembrane electrochemical gradient / xenobiotic transport across blood-brain barrier / Neurotransmitter clearance / Ciprofloxacin ADME / 神経伝達物質輸送体 / xenobiotic transport / xenobiotic transmembrane transporter activity / transport across blood-brain barrier / lateral plasma membrane / xenobiotic metabolic process / basal plasma membrane / presynapse / basolateral plasma membrane / apical plasma membrane / 生体膜 / identical protein binding / 細胞膜 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) Lama glama (ラマ) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.26 Å | ||||||
データ登録者 | Zhang, S. / Zhu, A. / Kong, F. / Chen, J. / Lan, B. / He, G. / Gao, K. / Cheng, L. / Yan, C. / Chen, L. / Liu, X. | ||||||
資金援助 | 中国, 1件
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引用 | ジャーナル: Cell Discov / 年: 2024 タイトル: Structural insights into human organic cation transporter 1 transport and inhibition. 著者: Shuhao Zhang / Angqi Zhu / Fang Kong / Jianan Chen / Baoliang Lan / Guodong He / Kaixuan Gao / Lili Cheng / Xiaoou Sun / Chuangye Yan / Ligong Chen / Xiangyu Liu / 要旨: The human organic cation transporter 1 (hOCT1), also known as SLC22A1, is integral to hepatic uptake of structurally diversified endogenous and exogenous organic cations, influencing both metabolism ...The human organic cation transporter 1 (hOCT1), also known as SLC22A1, is integral to hepatic uptake of structurally diversified endogenous and exogenous organic cations, influencing both metabolism and drug pharmacokinetics. hOCT1 has been implicated in the therapeutic dynamics of many drugs, making interactions with hOCT1 a key consideration in novel drug development and drug-drug interactions. Notably, metformin, the frontline medication for type 2 diabetes, is a prominent hOCT1 substrate. Conversely, hOCT1 can be inhibited by agents such as spironolactone, a steroid analog inhibitor of the aldosterone receptor, necessitating a deep understanding of hOCT1-drug interactions in the development of new pharmacological treatments. Despite extensive study, specifics of hOCT1 transport and inhibition mechanisms remain elusive at the molecular level. Here, we present cryo-electron microscopy structures of the hOCT1-metformin complex in three distinct conformational states - outward open, outward occluded, and inward occluded as well as substrate-free hOCT1 in both partially and fully open states. We also present hOCT1 in complex with spironolactone in both outward and inward facing conformations. These structures provide atomic-level insights into the dynamic metformin transfer process via hOCT1 and the mechanism by which spironolactone inhibits it. Additionally, we identify a 'YER' motif critical for the conformational flexibility of hOCT1 and likely other SLC22 family transporters. Our findings significantly advance the understanding of hOCT1 molecular function and offer a foundational framework for the design of new therapeutic agents targeting this transporter. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 8jty.cif.gz | 120.3 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb8jty.ent.gz | 90.3 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 8jty.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/jt/8jty ftp://data.pdbj.org/pub/pdb/validation_reports/jt/8jty | HTTPS FTP |
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-関連構造データ
関連構造データ | 36656MC 8jtsC 8jttC 8jtvC 8jtwC 8jtxC 8jtzC 8ju0C M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
-集合体
登録構造単位 |
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1 |
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-要素
#1: タンパク質 | 分子量: 62569.164 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: SLC22A1, OCT1 発現宿主: Spodoptera frugiperda (ツマジロクサヨトウ) 参照: UniProt: O15245 |
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#2: 抗体 | 分子量: 14421.984 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Lama glama (ラマ) / 発現宿主: Escherichia coli (大腸菌) |
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 | 名称: human organic cation transporter in compelx wtih nb5660 in inward facing partially open conformationOrganic cation transport protein タイプ: COMPLEX / Entity ID: all / 由来: MULTIPLE SOURCES |
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分子量 | 実験値: NO |
由来(天然) | 生物種: Homo sapiens (ヒト) |
由来(組換発現) | 生物種: Spodoptera frugiperda (ツマジロクサヨトウ) |
緩衝液 | pH: 8 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
-電子顕微鏡撮影
顕微鏡 | モデル: FEI TITAN |
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電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELDBright-field microscopy / 最大 デフォーカス(公称値): 1500 nm / 最小 デフォーカス(公称値): 1100 nm |
撮影 | 電子線照射量: 50 e/Å2 フィルム・検出器のモデル: FEI FALCON IV (4k x 4k) |
-解析
CTF補正 | タイプ: NONE | ||||||||||||||||||||||||
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3次元再構成 | 解像度: 3.26 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 132432 / 対称性のタイプ: POINT | ||||||||||||||||||||||||
拘束条件 |
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